CN100554426C - 用遗传修饰的谷氨酸棒杆菌生产l-赖氨酸 - Google Patents

用遗传修饰的谷氨酸棒杆菌生产l-赖氨酸 Download PDF

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CN100554426C
CN100554426C CNB028154460A CN02815446A CN100554426C CN 100554426 C CN100554426 C CN 100554426C CN B028154460 A CNB028154460 A CN B028154460A CN 02815446 A CN02815446 A CN 02815446A CN 100554426 C CN100554426 C CN 100554426C
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gene
lysc
bar shaped
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nucleotide sequence
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CN1539015A (zh
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布里吉特·巴瑟
卡罗琳·赖内恩
贝蒂娜·默克尔
格奥尔格·蒂尔巴赫
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Evonik Operations GmbH
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Tropical Medicine & Parasitology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CNB028154460A 2001-08-06 2002-07-30 用遗传修饰的谷氨酸棒杆菌生产l-赖氨酸 Expired - Fee Related CN100554426C (zh)

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US30987801P 2001-08-06 2001-08-06
US60/309,878 2001-08-06

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CN1539015A CN1539015A (zh) 2004-10-20
CN100554426C true CN100554426C (zh) 2009-10-28

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EP (1) EP1414970A2 (de)
CN (1) CN100554426C (de)
AU (1) AU2002325923A1 (de)
BR (1) BR0211723A (de)
CA (1) CA2455878A1 (de)
WO (1) WO2003040373A2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111471638A (zh) * 2020-05-22 2020-07-31 江南大学 一株产l-高丝氨酸的谷氨酸棒杆菌突变株的构建与应用

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DE10359594A1 (de) 2003-12-18 2005-07-28 Basf Ag PEF-TU-Expressionseinheiten
DE102004035067A1 (de) * 2004-07-20 2006-02-16 Basf Ag P1-35-Expressionseinheiten
DE102005013676A1 (de) 2005-03-24 2006-09-28 Degussa Ag Allele des zwf-Gens aus coryneformen Bakterien
DE102005023829A1 (de) 2005-05-24 2006-11-30 Degussa Ag Allele des opcA-Gens aus coryneformen Bakterien
DE102005047596A1 (de) 2005-10-05 2007-04-12 Degussa Ag Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien
DE102006026328A1 (de) * 2006-06-02 2008-01-03 Evonik Degussa Gmbh Verfahren zur Herstellung eines L-Lysin enthaltenden Futtermitteladditivs
KR100830289B1 (ko) * 2007-01-18 2008-05-16 씨제이제일제당 (주) Ｌ－아르기닌 생산 변이주 및 이의 제조방법
DE102007005072A1 (de) 2007-02-01 2008-08-07 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von Cadaverin
JP2011067095A (ja) 2008-01-10 2011-04-07 Ajinomoto Co Inc 発酵法による目的物質の製造法
KR100987281B1 (ko) * 2008-01-31 2010-10-12 씨제이제일제당 (주) 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법
DE102008001874A1 (de) 2008-05-20 2009-11-26 Evonik Degussa Gmbh Verfahren zur Herstellung von L-Aminosäuren
DE102009030342A1 (de) 2009-06-25 2010-12-30 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von organisch chemischen Verbindungen
DE102010003419B4 (de) 2010-03-30 2019-09-12 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von L-Ornithin
DE102010025124A1 (de) 2010-06-25 2011-12-29 Forschungszentrum Jülich GmbH Verfahren zur Herstellung von D-Aminosäuren, Mikroorganismus, sowie Vektor
EP2479279A1 (de) 2011-01-20 2012-07-25 Evonik Degussa GmbH Verfahren zur fermentativen Herstellung schwefelhaltiger Aminosäuren
CN103492553B (zh) 2011-02-22 2018-06-12 巴斯夫欧洲公司 用于生产尸胺的方法和重组微生物
DE102011006716A1 (de) 2011-04-04 2012-10-04 Evonik Degussa Gmbh Mikroorganismus und Verfahren zur fermentativen Herstellung einer organisch-chemischen Verbindung
JP2014131487A (ja) 2011-04-18 2014-07-17 Ajinomoto Co Inc Ｌ−システインの製造法
DE102011118019A1 (de) 2011-06-28 2013-01-03 Evonik Degussa Gmbh Varianten des Promotors des für die Glyzerinaldehyd-3-phosphat-Dehydrogenase kodierenden gap-Gens
CN103946372B (zh) 2011-11-02 2016-06-08 味之素株式会社 用于分泌产生蛋白质的方法
CN103930557A (zh) 2011-11-11 2014-07-16 味之素株式会社 利用发酵法制造目标物质的方法
EP2628792A1 (de) 2012-02-17 2013-08-21 Evonik Industries AG Zelle mit verringerter ppGppase-Aktivität
EP2631298A1 (de) 2012-02-22 2013-08-28 Evonik Industries AG Biotechnologisches Verfahren zur Herstellung von Butanol und Buttersäure
ES2666072T3 (es) 2012-04-27 2018-04-30 Evonik Technochemie Gmbh Alfa-isopropilmalato sintasas resistentes a la retroacción
EP2674489A1 (de) 2012-06-15 2013-12-18 Evonik Industries AG Biotechnologische Herstellung von 2-hydroxyisobuttersäure
EP2700715B1 (de) 2012-08-20 2018-07-25 Evonik Degussa GmbH Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung von verbesserten Stämmen der Familie Enterobacteriaceae
DE102012016716A1 (de) 2012-08-22 2014-02-27 Forschungszentrum Jülich GmbH Verfahren zur Herstellung von Vektoren enthaltend ein für in seiner feedback-Inhibierung gemindertes oder ausgeschaltetes Enzym kodierendes Gen und deren Verwendung für die Herstellung von Aminosäuren und Nukleotiden
EP2762571A1 (de) 2013-01-30 2014-08-06 Evonik Industries AG Mikroorganismus und Verfahren zur fermentativen Herstellung von Aminosäuren
EP2811028B1 (de) 2013-06-03 2017-02-01 Evonik Degussa GmbH Verfahren zur Herstellung von L-Valin unter Verwendung rekombinanter Corynebakterien enthaltend das durch Propionat induzierbare ilvBN-Operon
CN103374540B (zh) * 2013-08-13 2015-04-22 廊坊梅花生物技术开发有限公司 谷氨酸棒杆菌及其应用
DE102014208199A1 (de) 2014-04-30 2015-11-05 Evonik Degussa Gmbh Verfahren zur Produktion von L-Aminosäuren unter Verwendung eines alkaliphilen Bakteriums
EP2940039A1 (de) 2014-04-30 2015-11-04 Evonik Degussa GmbH Verfahren zur Produktion von L-Aminosäuren in Corynebakterien unter Verwendung eines Glycin spaltenden Systems
EP2940144A1 (de) 2014-04-30 2015-11-04 Evonik Degussa GmbH Verfahren zur Produktion von L-Lysin unter Verwendung eines alkaliphilen Bakteriums
EP2940143B1 (de) 2014-04-30 2020-02-05 Evonik Operations GmbH Verfahren zur Produktion von L-Aminosäuren unter Verwendung eines alkaliphilen Bakteriums
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
CA3007635A1 (en) 2015-12-07 2017-06-15 Zymergen Inc. Promoters from corynebacterium glutamicum
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
KR101756338B1 (ko) * 2016-01-15 2017-07-10 고려대학교 산학협력단 L-시스테인 생산용 변이미생물 및 이를 이용한 l-시스테인의 제조방법
CN105838740B (zh) * 2016-05-03 2020-11-06 湖北大学 一种茶树内生草螺菌制备纳米红色元素硒的方法
KR102345898B1 (ko) 2016-06-30 2022-01-03 지머젠 인코포레이티드 글루코오스 투과 효소 라이브러리를 생성하는 방법 및 이의 용도
WO2018005655A2 (en) 2016-06-30 2018-01-04 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
RU2760290C1 (ru) * 2016-07-08 2021-11-23 Метаболик Эксплорер Способ ферментативной продукции целевых молекул микроорганизмами, включающими гены, кодирующие фосфотрансферазную систему (фтс) сахаров
CN106635944A (zh) * 2016-12-29 2017-05-10 廊坊梅花生物技术开发有限公司 一种谷氨酸棒杆菌及其构建方法与应用
KR20200026881A (ko) 2017-06-07 2020-03-11 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터 및 보조 유전자 발현을 조절하는 데 이의 용도
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111471638A (zh) * 2020-05-22 2020-07-31 江南大学 一株产l-高丝氨酸的谷氨酸棒杆菌突变株的构建与应用
CN111471638B (zh) * 2020-05-22 2021-11-23 江南大学 一株产l-高丝氨酸的谷氨酸棒杆菌突变株的构建与应用

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CA2455878A1 (en) 2003-05-15
WO2003040373A3 (en) 2003-12-18
WO2003040373A2 (en) 2003-05-15
CN1539015A (zh) 2004-10-20
AU2002325923A1 (en) 2003-05-19
BR0211723A (pt) 2004-09-21
EP1414970A2 (de) 2004-05-06

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