CN100525800C - Composition of Chinese traditional medicine for treating yellow / white flux of baby pig, and colibacillosis of birds - Google Patents
Composition of Chinese traditional medicine for treating yellow / white flux of baby pig, and colibacillosis of birds Download PDFInfo
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Abstract
A composite Chinese veterinary medicine for preventing and treating the dysentery of piglet and the colibacillosis of fowls is prepared from 8 Chinese-medicinal materials including scutellaria root, cherokee rose-hip, sanguisorba root, lucid ligustrum fruit, etc through extracting in water, decocting twice, adsorbing and drying.
Description
Technical field
The present invention relates to natural medicine field for animals, be specifically related to a kind of natural drug compound, it can be used as feed additive and promotes piglet growth, and yellow and white dysentery of piglet and avian colibacillosis are had the better prevention effect.
Background technology
China delivered 5.86 hundred million of barren sows in 2003 for sale, accounted for the world and delivered 47.1% of pig for sale, and 4,607 ten thousand tons of Carnis Sus domestica output account for 46.79% of world's Carnis Sus domestica output, and Carnis Sus domestica 35.38kg exceeds more than 1 times of world's per capita share (15.82kg) per capita.2004, China's livestock on hand pig number reached 4.82 hundred million.National poultry livestock on hand was 53.5 hundred million in 2005, delivered 96.7 hundred million for sale, all occupied first place in the world, and increased by 15.5% and 19.4% respectively than 2000.
But along with developing rapidly of aquaculture, breed scientific management, diseases prevention and treatment do not catch up with the development of aquaculture, have brought serious strike to aquaculture.Disease popularity is frequent day by day, and often chronic illness does not eliminate, and new disease increases again, and is hard to guard against, brought serious strike to aquaculture.If be used for the treatment of the drug main antibiotic of fowl poultry intestinal tract disease at present, as gentamycin etc.
China abuses one of more serious country of antibiotics in the world, and the human medicine still is that animal-use drug all exists the abuse problem.The bacterial drug resistance problem that causes thus is very outstanding.The clinical more isolating antibacterials of China have been soared to first place, the world to the drug resistance total quantity (for example intestinal is for the drug resistance of quinolone antibiotic) of some drugs.For a long time, the Western medicine veterinary drug is propagated its belief on a large scale in plant with its curative effect height, instant effect, and is in fashion for a time.Yet the drug resistance that the thing followed is serious day by day, curative effect reduction, drug residue and pollution problem but make and culture rather headache of boundary.The ban that the various limit drug of authoritative institution is residual, and the enhancing of ordinary consumer environmental consciousness and health perception, making has the meat product market of drug residue obviously to be obstructed, and the economic interests of plant are on the hazard.As far back as 1998, European Commission EC just issued at the ban in European Union's scope of 4 kinds of antibiotic growth promoter-bacitracin zincs, spiramycin, Virginia mycin and tylosin phosphate.Afterwards, European Union has issued ban to more antibiotic in succession.Several years ago, U.S. food and drug administration (FDA) warning U.S. feed manufacturer stops using unauthorized antibiotic in fish meal.Ban use of antibiotic scope to enlarge in the U.S. feed at present, relate to main edible animal pig, poultry and cattle.To careful use veterinary drug problem, China has also issued a series of regulations, but in actual production illegal use and the abuse of antibiotics problem very serious, people and animals' medicine is used with also very general.
The abuse of antibiotic and chemical drugs causes environmental pollution, and drug residue has influence on the food-safety problem of consumer, serious all human beings'health that jeopardize.Economic interests are ordered about, some phenomenons coastal and the super scope overdose Drug abuse additive of animal husbandry developed regions existence, and the residual human body food poisoning that causes of forbidden drug happens occasionally, and remains incessant after repeated prohibition.The focus that the topic that Drug abuse additive high residue causes the human body drug resistance to shift has become industry scientific and technical personnel to pay close attention to and study also causes the extensive concern of society and people's huge fear, and the prices fluctuate much to make livestock products.On the consumption market, carnivorous drug residue has seriously suppressed the steady growth of carnivorous consumption to a certain extent at home.In recent years, China's livestock products outlet seriously is obstructed, and European Union, the U.S., Japan, Korea S flash the redlight to China's outlet meat product again and again, and residual the exceeding standard of the carnivorous Chinese medicine that the veterinary drug improper use causes is one of the main reasons.A lot of plants have paid heavy cost for this reason.
Summary of the invention
The invention discloses a kind of natural drug composition, it can be used for preventing and treating fowl poultry intestinal tract disease, can also promote the growth of fowl poultry simultaneously.
Natural drug composition of the present invention is made up of eight flavor Chinese medicines: Radix Scutellariae, Herba Acalyphae, Fructus Rosae Laevigatae, Herba Euphorbiae Humifusae, Herba Erodii, Radix Sanguisorbae, Radix Scrophulariae and Fructus Ligustri Lucidi.Eight flavor Chinese medicines preferred ratios of weight and number are: 1-5 parts of 2-6 parts of Radix Scutellariaes, 1-5 parts of Herba Acalyphaes, 1-5 parts of Fructus Rosae Laevigatae, 1-5 parts of Herba Euphorbiae Humifusaes, 1-5 parts of Herba Erodiis, 1-5 parts of Radix Sanguisorbaes, 1-5 parts of Radix Scrophulariaes and Fructus Ligustri Lucidi.
Above-mentioned natural drug composition can be directly used in feedstuff and always feed, and preferred manufacturing procedure is: with medicinal hot water extraction, concentrated getting final product in eight flavors.Also can be promptly with the compositions after concentrating and carrier such as white carbon black mixing after drying.In order to prevent that eight flavor Chinese medicines between the extract chemical reaction can take place when all mixing decocting in water, cause the product active constituent content to reduce, therefore the present invention preferably is divided into two components with eight flavor Chinese medicines and gets indescribably, one group is Radix Scutellariae, Herba Acalyphae, Fructus Rosae Laevigatae and Herba Euphorbiae Humifusae, another group is Herba Erodii, Radix Sanguisorbae, Radix Scrophulariae and Fructus Ligustri Lucidi, uses hot water extraction respectively for two groups, concentrates the back remix, so separately extraction can obtain the highest effective ingredient of concentration, the performance maximum effect.Before extracting, preferably soak, its objective is to make medical material organize moistening infiltration, in order to the dissolving and the leaching of effective ingredient.Decocting time was selected greater than 0.5 hour, generally about 1～3 hour, in order to improve the extract productive rate, can select secondary to decoct, and secondary decocts more can fully extract middle pharmaceutically active ingredient.
Natural drug composition of the present invention is efficient, the stable prophyiaxis and promoting growth additive that extracts from natural Chinese medicinal herb, contains active ingredients such as multiple alkaloid, triterpenoid compound, glycoside, flavone, has antibiotic, antiviral, enhancing human body immunity function.Show good diseases prevention and growth promotion effect in the test of piglet, this effect has also obtained checking in the test of broiler and laying hen.Be part test and result below:
One, natural drug composition of the present invention is to the influence of suckling piglet fertility performance
1 materials and methods
1.1 natural drug composition of the present invention (hereinafter to be referred as the joy treasured), enteric Kitasamycin 500 granular preparations, kangdisu sulfas 100, olaquindox, 4% premix material composition see Table 2; Piglet is Du * length * Dasanyuan hybridized pig.
1.2 experimental animal and EXPERIMENTAL DESIGN: selecting with 9 of the sows of kind, according to close principle of parity, expected date of confinement, is a repetition with every nest piglet, and every group of 3 repetitions are divided into A, B, three groups of C.With the A group is matched group, adds Kitasamycin, kangdisu sulfas and olaquindox; B, C group is added 350ppm, 500ppm (concentration of medicine in feedstuff) joy treasured (seeing Table 1) respectively for test group, and test pig adopts identical daily ration to handle.
Table 1 test is handled
| Test number | A | B | C |
| Test is handled | Kitasamycin 40ppm+ kangdisu sulfas 50ppm+ olaquindox 50ppm | The precious 350ppm of joy | The precious 500ppm of joy |
| Test sow number | 3 | 3 | 3 |
| Test piglet number | 28 | 26 | 26 |
The feed joy treasured of basal diet+various dose of basal diet+antibiotic 1.3 test diet: A group is fed, B, C.The experimental basis daily ration is formed and nutrition sees Table 2
Table 2 experimental basis daily ration
1.4 feeding and management:
1.4.1 artificial the throwing raised powder, free choice feeding is freely drunk water; The wean of piglet 25 ages in days; To 38 age in days off-tests;
1.4.2 the personal management, each group is carried out under identical feeding and management and environmental condition;
1.4.3 observe and record: every day the viewing test pig search for food, diarrhoea and active situation, and carry out record.When on-test, wean and end, respectively test pig is carried out full group and weigh.
2 test results and analysis
2.1 test pig body weight and equal daily gain situation see Table 3
Table 3 each test group body weight and weightening finish
| Group | The A group | The B group | The C group |
| 7 age in days counterpoises (kg/ head) | 2.80 | 2.73 | 2.89 |
| 25 age in days weanling weights (kg/ head) | 7.35 | 7.28 | 7.11 |
| 38 age in days body weight (kg/ head) | 11.83 | 12.17 | 12.23 |
| 7～25 ages in days weightening finishes (kg/ head) | 4.55 | 4.55 | 4.22 |
| 25～38 ages in days weightening finishes (kg/ head) | 4.48 | 4.89 | 5.12 |
At 7～25 ages in days, three groups of average daily gains are 253g, 253g, 234g, and C group may be relevant with the milk water quality of sow a little less than A, B group because this in stage piglet based on sucking; 25～38 ages in days, three groups of average daily gains are respectively 320g, 349g, 366g, and the precious group of joy daily gain is better than the antibiotic group, improves 9.06% and 14.38% than antibiotic group respectively; As if gaining effect is proportional with the precious additive capacity of joy, shows that the joy treasured has special advantages aspect the prevention suckling piglet ablactation stress.
2.2 influence to pig starter feed efficient
By the equal feed consumption of visible 7～25 age in days piglet heads of table 4 is 292.31g～321.15g, and material anharmonic ratio 0.065～0.071: 1 does not have significant difference between each group, and piglet is main relevant with the sucking stage therewith.25～38 ages in days, C group feed consumption rate are apparently higher than A, B group, and the B group is a little more than the A group, and the precious group material of joy anharmonic ratio obviously is better than antibiotic group (A group), reduces by 7.43%, 8.78% than antibiotic group (A group) respectively; Above results suggest joy treasured has appetite stimulator, improves feed intake, improves the effect of piglet fertility performance.
Table 4 is respectively organized food consumption and conversion ratio unit: the g/ head
| Group | The A group | The B group | The C group |
| 7～25 age in days feed consumption rates | 296.43 | 321.15 | 292.31 |
| 7～25 age in days material anharmonic ratioes | 0.065∶1 | 0.071∶1 | 0.069∶1 |
| 25 ages in days～38 age in days feed consumption rates | 6630.47 | 6699.32 | 6912.24 |
| 25 ages in days～38 age in days material anharmonic ratioes | 1.48∶1 | 1.37∶1 | 1.35∶1 |
2.3 duration of test piglets diarrhoea situation sees Table 5
Table 5 a piglets diarrhoea number of days and diarrhea rate
| Group | A | B | C |
| The piglet number | 28 | 26 | 26 |
| 7～25 an ages in days diarrhoea number | 14 | 15 | 5 |
| ∑ 7～25 ages in days are raised a number of days | 504 | 468 | 468 |
| 7～25 age in days diarrhea rates | 2.78％ | 3.21％ | 1.07％ |
| 25～38 an ages in days diarrhoea number of days | 4 | 3 | 1 |
| ∑ 25～38 ages in days are raised a number of days | 392 | 364 | 364 |
| 25～38 age in days diarrhea rates (%) | 1.02％ | 0.82％ | 0.27％ |
| ∑ is tested a full phase diarrhoea number of days | 18 | 18 | 6 |
| ∑ is tested the full phase and is raised a number of days | 896 | 832 | 832 |
| Full phase diarrhea rate (%) | 2.01％ | 2.16％ | 0.72％ |
This test data shows that the joy treasured can replace antibiotic aspect the prevention suckling piglet diarrhoea fully, and each group wean back diarrhea rate is all lower, and in 25～38 age in days weaning periods, the effect of the precious control of joy piglet diarrhea is better than antibiotic especially.
3 results and discussion
Chinese herbal medicine is run concurrently and is supported and medicinal double attribute, except certain nutrient value, mainly contains antibacterial activity or other biological activity, and Chinese herbal feed additive research has obtained application result preferably in China.The joy treasured is multiple Chinese herbal medicine extract, this test shows, add Chinese herbal medicine extract-joy treasured in the piglet diet and can prevent suckling piglet diarrhoea preferably, promote the suckling piglet growth, improve feed efficiency, during weaned piglet, its weightening finish is better than antibiotic with anti-diarrhoea effect, is first-selected at present antibiotic succedaneum especially.
Two, natural drug composition of the present invention is to the fertility performance influence of laying hen
1 test material and method
1.1 experimental animal and EXPERIMENTAL DESIGN:
Select 800 plumage body weight close 49 the week age Luo Man laying hen, be divided into 4 groups at random, establish 4 repetitions for every group, each repeats 50 plumages.Testing the full phase amounts to 6 weeks (comprising 7 days phases of raising in advance).Test grouping situation sees Table 6 wherein, and matched group is blank group, and test I, II, III group are the precious gradient group of joy.
Table 6 test grouping situation
| Group | Matched group | Test I group | Test II group | Test III group |
| Handle | Blank | The precious 100ppm of joy | The precious 150ppm of joy | The precious 200ppm of joy |
| Repeat number * chicken number | 4×50 | 4×50 | 4×50 | 4×50 |
1.2 feeding and management:
Just the examination phase begins fertility performance and health status zero difference between preceding group.Powder free choice feeding, artificial feeding, nipple-shape automatic drinking bowl drinking-water.Semi open model is hen house fixedly, and three layers of full ladder of biserial are raised in cages, 4 chickens of every cage.Every day, egg was chosen in 2 craft.Feeding and management maintenance and jumpbogroup are produced in full accord.
1.3 test daily ration prescription:
The experimental basis daily ration: the material type is a powdery, and its feed formula sees Table 7
Table 7 daily ration prescription is formed
1.4 observation index and date processing:
Write down amount of livestock on hand every day, the sum of laying eggs, total egg size, food consumption and the dead number of washing in a pan.Calculate laying rate, egg size, the price of deed, death rate.Data are carried out single factor analysis.
2. interpretation of result and discussion
Laying rate is of paramount importance index during laying hen produces all the time.The result shows the joy treasured that adds various dose, all can improve the laying rate (see figure 1) in various degree, the test group laying rate is higher than matched group all the time in the experimental period in 5 weeks, the precious effect of 150ppm and 200ppm joy is better than 100ppm, and it is close with the 200ppm effect to add 150ppm.
Result of the test shows that the joy treasured improves the price of deed to improving laying rate of laying hen, and all there is good effect aspects such as reduction death rate.
Three, natural drug composition of the present invention is to the influence of meat chicken production performance
1 materials and methods
1.1 test chicken group: select young 600 of healthy active, normotrophic AA meat, be divided into three groups at random, establish four repetitions for every group, 50 of every repetitions.If test I group (the precious group of joy), test II group (antibiotic group: colistine sulfate and bacitracin zinc), matched group.
1.2 test daily ration: the experimental basis daily ration is preparation voluntarily, adds " joy treasured " 200ppm in the test I group basal diet, adds colistine sulfate 20ppm, bacitracin zinc 50ppm, the control group fed basal diet in the test II group basal diet.Basal diet and trophic level see Table 8.
Table 8 basal diet
1.3 feeding and management: raise on test broiler plane, the secondary that feeds intake every day, and free choice feeding is freely drunk water, and the immunity epidemic prevention is undertaken by normal procedure, and each group is raised term harmonization.Write down feed consumption rate, the death toll of test chicken every day, be that unit carries out empty stomach and weighs morning with the repeating groups in on-test, when finishing.
1.4 date processing and analysis
Data are carried out the SPSS11.5 statistical analysis after Excel 2000 handles, data are average ± standard error (x ± SE).
2 test results and analysis
2.1 the joy treasured is to the influence of meat chicken production performance
Duration of test, each organizes the chicken physical health, the no abnormal situation of appetite.Broiler zero difference between the body weight group when 0 age in days as shown in Table 9.During off-test (21 age in days), the precious group of joy, antibiotic group, matched group body weight are respectively 668.3g, 661g, 629.9g, significant difference (P＜0.05).The precious group of joy and antibiotic group weightening finish comparison according to component you can well imagine high by 6.56%, 5.28%, the precious group of joy gaining effect is better than the antibiotic group, but group difference not significantly (P〉0.05).
Table 9 joy treasured is to the influence of 0～21 age in days broiler chicken fertility performance
Annotate: different letter representation significant differences (P＜0.05) are arranged in the same column data.
2.2 the joy treasured is to the influence of broiler fodder conversion ratio and survival rate
Result of the test (table 10) shows that the precious group material of joy anharmonic ratio is 1.41: 1, and antibiotic group material anharmonic ratio is 1.40: 1, and matched group material anharmonic ratio is 1.49: 1, and two groups have descended 5.37%, 6.04% than matched group respectively.The joy treasured reaches the effect similar to the antibiotic group.The precious composition of joy motility rate is 97.5%, and antibiotic is formed motility rate 98%, matched group survival rate 93.5%, and joy treasured, antibiotic all can obviously improve survival rate.Find out that thus the joy treasured has good disease-resistant and prophylaxis effect, and can substitute antibiotic application in broiler is produced fully.
Table 10 joy treasured is to the influence of 0～21 age in days broiler chicken feed conversion rate and survival rate
Description of drawings
Fig. 1 is the influence of natural drug composition of the present invention to laying rate
The specific embodiment
Embodiment 1
1 part of 1 part of Herba Euphorbiae Humifusae of 1 portion of Fructus Rosae Laevigatae of 2 parts of Herba Acalyphaes of Radix Scutellariae
1 part of 1 part of Fructus Ligustri Lucidi of 1 portion of Radix Scrophulariae of 1 portion of Radix Sanguisorbae of Herba Erodii
Preparation method:
Pretreatment with Chinese medicine: carry out pretreatment to extracting used Chinese crude drug, promptly clean, clean and drain, and Chinese crude drug is divided into two groups by 1# (Radix Scutellariae, Herba Acalyphae, Fructus Rosae Laevigatae, Herba Euphorbiae Humifusae) and 2# (Herba Erodii, Radix Sanguisorbae, Radix Scrophulariae, Fructus Ligustri Lucidi).
One, extraction concentrates:
1, Chinese medicine extraction liquid 1# extracts and concentrates:
(1) checks at first whether the extraction pot valve closes (residual cake valve, bleeder valve), then with Radix Scutellariae, Herba Acalyphae, Fructus Rosae Laevigatae, Herba Euphorbiae Humifusae feeds intake, by material, water added behind the water merceration 3 hours than 1: 5, open Steam Heating, to decoct 2 hours so that 100-110 ℃ temperature is lasting again behind the water boil in the extraction pot, steam is closed vexed 4 hours (during the medicinal liquid in the extraction pot was once carried out periodicity in per 30 minutes with feed pump force outer circulation), then with extracting solution suction fluid reservoir, then again by material, water added water to carry out two and fries in shallow oil than 1: 1, decocted 1 hour, and emitted medicinal residues after waiting to extract medicinal liquid.
(2) close the concentration tank bleeder valve, open recirculated cooling water, the preceding inlet valve of pump again, open inlet valve and feed pump then, the medicinal liquid of fluid reservoir is got in the concentration tank, level control is under manhole about 10cm, close feed pump and inlet valve then, open steam, open vacuum pump, carry out concentrating under reduced pressure (observe feed liquid at set intervals,, must progressively feed in raw material) then if reduce, elder generation's steam off, vacuum pump during discharging, then open air valve to normal pressure, open the material-compound tank inlet valve again, open the material-compound tank feed pump at last and begin discharge.
2, Chinese medicine extraction liquid 2# extracts and concentrates:
Press Chinese medicine extraction liquid 2# prescription neck material, operate by the extraction concentration process of above-mentioned Chinese medicine extraction liquid 1#.
Two, composite:
Treat that Chinese medicine extraction liquid 1# and Chinese medicine extraction liquid 2# squeeze in the material-compound tank, open stirring motor and stir, and whether will open steam insulation or heating, wait to emit after stirring and contain all turning barrels according to temperature decision in season.
Three, absorption oven dry:
White carbon is added trough type mixing machine, open trough type mixing machine, with watering pot composite Chinese medicine extraction liquid is sprayed into while stirring, treat the absorption baking oven of packing into after good, oven temperature is no more than 90 ℃, and moisture content of finished products is controlled at below 5% after the baking.
Four, the packing of sieving:
Semi-finished product after the oven dry are sieved in shaking screen, then compound evenly after, the vanning warehouse-in.
Embodiment 2
5 parts of 5 parts of Herba Euphorbiae Humifusaes of 5 portions of Fructus Rosae Laevigatae of 6 parts of Herba Acalyphaes of Radix Scutellariae
5 parts of 5 parts of Fructus Ligustri Lucidi of 5 portions of Radix Scrophulariaes of 5 portions of Radix Sanguisorbaes of Herba Erodii
Preparation method is with embodiment 1.
Embodiment 3
3 parts of 3 parts of Herba Euphorbiae Humifusaes of 3 portions of Fructus Rosae Laevigatae of 4 parts of Herba Acalyphaes of Radix Scutellariae
3 parts of 3 parts of Fructus Ligustri Lucidi of 3 portions of Radix Scrophulariaes of 3 portions of Radix Sanguisorbaes of Herba Erodii
Preparation method is with embodiment 1.
Embodiment 4
2 parts of 2 parts of Herba Euphorbiae Humifusaes of 2 portions of Fructus Rosae Laevigatae of 3 parts of Herba Acalyphaes of Radix Scutellariae
2 parts of 2 parts of Fructus Ligustri Lucidi of 2 portions of Radix Scrophulariaes of 2 portions of Radix Sanguisorbaes of Herba Erodii
Preparation method is with embodiment 1.
Embodiment 5
4 parts of 4 parts of Herba Euphorbiae Humifusaes of 4 portions of Fructus Rosae Laevigatae of 5 parts of Herba Acalyphaes of Radix Scutellariae
4 parts of 4.5 parts of Fructus Ligustri Lucidi of 4 portions of Radix Scrophulariaes of 4 portions of Radix Sanguisorbaes of Herba Erodii
Preparation method is with embodiment 1.
Claims (5)
1, a kind of Chinese medicine composition of preventing and treating fowl poultry digestive tract disease is characterized in that being formed by the feedstock production of following weight ratio:
1-5 parts of 1-5 parts of 2-6 parts of Radix Scutellariaes, 1-5 parts of Herba Acalyphaes, 1-5 parts of Fructus Rosae Laevigatae, 1-5 parts of Herba Euphorbiae Humifusaes, 1-5 parts of Herba Erodiis, 1-5 parts of Radix Sanguisorbaes, Radix Scrophulariae and Fructus Ligustri Lucidi.
2, the preparation method of the Chinese medicine composition of claim 1 comprises: will in medicinal hot water extraction, concentrate, be drying to obtain.
3, the preparation method of claim 2, wherein be one group and mix the back hot water extraction Radix Scutellariae, Herba Acalyphae, Fructus Rosae Laevigatae and Herba Euphorbiae Humifusae, Herba Erodii, Radix Sanguisorbae, Radix Scrophulariae and Fructus Ligustri Lucidi are one group mix the back hot water extraction that extracting solution concentrates respectively, dry back remix.
4, the Chinese medicine composition of claim 1 is used to prepare the purposes of the medicine for the treatment of pig HUANGBAI(sic) dysentery.
5, the Chinese medicine composition of claim 1 is as the purposes of feed additive.
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102657296B (en) * | 2012-05-25 | 2013-09-18 | 江西赣达牧业有限公司 | Traditional Chinese medicine health-care type feed for suckling pigs |
| CN104082529B (en) * | 2014-05-28 | 2016-05-11 | 张绪伟 | For preventing and treating the feed of piglet yellow-white dysentery |
| CN104543576B (en) * | 2015-01-16 | 2018-02-09 | 湖南沙博安五季丰生物科技有限公司 | Prevent and treat composite vegetables extractive feed addictive and its preparation method application of grice diarrhoea |
| CN106267153A (en) * | 2016-08-22 | 2017-01-04 | 广西大学 | A kind of poultry compound medicine containing Fructus Rosae Laevigatae and colistin |
| CN106344507A (en) * | 2016-11-17 | 2017-01-25 | 无锡奔牛生物科技有限公司 | Preparation method of radix scutellariae toxin-resolving powder oral liquid |
| CN109349425A (en) * | 2018-10-27 | 2019-02-19 | 江苏波杜农牧股份有限公司 | A kind of Chinese herbal feed additive and preparation method thereof |
| CN111011628A (en) * | 2019-12-19 | 2020-04-17 | 何先清 | Plant extract and preparation method and application thereof |
| CN111358850B (en) * | 2020-04-26 | 2022-04-19 | 河南牧翔动物药业有限公司 | Traditional Chinese medicine composition for preventing and treating chicken colibacillosis and preparation method thereof |
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