CN100473725C - 苏云金芽孢杆菌CryET33和CryET34组合物及其使用 - Google Patents
苏云金芽孢杆菌CryET33和CryET34组合物及其使用 Download PDFInfo
- Publication number
- CN100473725C CN100473725C CNB971800022A CN97180002A CN100473725C CN 100473725 C CN100473725 C CN 100473725C CN B971800022 A CNB971800022 A CN B971800022A CN 97180002 A CN97180002 A CN 97180002A CN 100473725 C CN100473725 C CN 100473725C
- Authority
- CN
- China
- Prior art keywords
- seq
- cell
- nucleic acid
- sequence
- bacillus thuringiensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000193388 Bacillus thuringiensis Species 0.000 title claims abstract description 115
- 229940097012 bacillus thuringiensis Drugs 0.000 title claims abstract description 114
- 239000000203 mixture Substances 0.000 title claims description 78
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 325
- 238000000034 method Methods 0.000 claims abstract description 138
- 150000007523 nucleic acids Chemical group 0.000 claims abstract description 61
- 230000000749 insecticidal effect Effects 0.000 claims abstract description 29
- 241000254101 Popillia japonica Species 0.000 claims abstract description 24
- 241000254113 Tribolium castaneum Species 0.000 claims abstract description 21
- 210000004027 cell Anatomy 0.000 claims description 189
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 135
- 241000196324 Embryophyta Species 0.000 claims description 132
- 230000001580 bacterial effect Effects 0.000 claims description 109
- 235000001014 amino acid Nutrition 0.000 claims description 67
- 150000001413 amino acids Chemical class 0.000 claims description 66
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 58
- 229920001184 polypeptide Polymers 0.000 claims description 55
- 108020004707 nucleic acids Proteins 0.000 claims description 53
- 102000039446 nucleic acids Human genes 0.000 claims description 53
- 239000000523 sample Substances 0.000 claims description 47
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 46
- 241000894006 Bacteria Species 0.000 claims description 45
- 239000002773 nucleotide Substances 0.000 claims description 42
- 125000003729 nucleotide group Chemical group 0.000 claims description 42
- 239000003153 chemical reaction reagent Substances 0.000 claims description 39
- 238000009396 hybridization Methods 0.000 claims description 39
- 230000014509 gene expression Effects 0.000 claims description 36
- 230000000295 complement effect Effects 0.000 claims description 29
- 238000002360 preparation method Methods 0.000 claims description 28
- 239000013598 vector Substances 0.000 claims description 22
- 235000013311 vegetables Nutrition 0.000 claims description 19
- 238000012360 testing method Methods 0.000 claims description 18
- 238000001514 detection method Methods 0.000 claims description 17
- 230000000968 intestinal effect Effects 0.000 claims description 17
- 240000008042 Zea mays Species 0.000 claims description 13
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims description 13
- 241000254175 Anthonomus grandis Species 0.000 claims description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 11
- 230000015572 biosynthetic process Effects 0.000 claims description 10
- 230000036039 immunity Effects 0.000 claims description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 241000589516 Pseudomonas Species 0.000 claims description 6
- 239000003053 toxin Substances 0.000 claims description 6
- 241000194107 Bacillus megaterium Species 0.000 claims description 5
- 241000209140 Triticum Species 0.000 claims description 5
- 235000021307 Triticum Nutrition 0.000 claims description 5
- 241000894007 species Species 0.000 claims description 5
- 235000013399 edible fruits Nutrition 0.000 claims description 3
- 230000036737 immune function Effects 0.000 claims description 3
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 2
- 239000012472 biological sample Substances 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 101710151559 Crystal protein Proteins 0.000 abstract description 117
- 230000009261 transgenic effect Effects 0.000 abstract description 51
- 241000238631 Hexapoda Species 0.000 abstract description 26
- 239000013078 crystal Substances 0.000 abstract description 22
- 108700012359 toxins Proteins 0.000 abstract description 13
- 231100000331 toxic Toxicity 0.000 abstract description 4
- 230000002588 toxic effect Effects 0.000 abstract description 4
- 102000004169 proteins and genes Human genes 0.000 description 145
- 235000018102 proteins Nutrition 0.000 description 142
- 108020004414 DNA Proteins 0.000 description 138
- 229940024606 amino acid Drugs 0.000 description 65
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 60
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 42
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 42
- 108091028043 Nucleic acid sequence Proteins 0.000 description 42
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 37
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 37
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 36
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 35
- 239000013612 plasmid Substances 0.000 description 35
- 230000002939 deleterious effect Effects 0.000 description 32
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 30
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 30
- 239000000427 antigen Substances 0.000 description 29
- 108091007433 antigens Proteins 0.000 description 29
- 102000036639 antigens Human genes 0.000 description 29
- 238000005516 engineering process Methods 0.000 description 29
- 108091026890 Coding region Proteins 0.000 description 27
- 239000012634 fragment Substances 0.000 description 25
- 241000193830 Bacillus <bacterium> Species 0.000 description 24
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 23
- 102000004190 Enzymes Human genes 0.000 description 22
- 108090000790 Enzymes Proteins 0.000 description 22
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 22
- 239000002585 base Substances 0.000 description 20
- 238000006243 chemical reaction Methods 0.000 description 20
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 19
- 241001465754 Metazoa Species 0.000 description 19
- 241000254173 Coleoptera Species 0.000 description 16
- 230000000875 corresponding effect Effects 0.000 description 16
- 238000011160 research Methods 0.000 description 16
- 210000001519 tissue Anatomy 0.000 description 16
- 238000012216 screening Methods 0.000 description 15
- 239000000725 suspension Substances 0.000 description 15
- 230000008859 change Effects 0.000 description 13
- 238000004520 electroporation Methods 0.000 description 13
- 239000002245 particle Substances 0.000 description 13
- 231100000419 toxicity Toxicity 0.000 description 13
- 230000001988 toxicity Effects 0.000 description 13
- 241000588724 Escherichia coli Species 0.000 description 12
- 244000061456 Solanum tuberosum Species 0.000 description 12
- 235000002595 Solanum tuberosum Nutrition 0.000 description 12
- 230000000694 effects Effects 0.000 description 12
- 210000004408 hybridoma Anatomy 0.000 description 12
- 239000000126 substance Substances 0.000 description 12
- 230000006870 function Effects 0.000 description 11
- 230000008569 process Effects 0.000 description 11
- 230000008901 benefit Effects 0.000 description 10
- 239000013604 expression vector Substances 0.000 description 10
- 239000000499 gel Substances 0.000 description 10
- 230000008929 regeneration Effects 0.000 description 10
- 238000011069 regeneration method Methods 0.000 description 10
- 230000028070 sporulation Effects 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 9
- 101000878902 Bacillus thuringiensis Pesticidal crystal protein Cry6Aa Proteins 0.000 description 9
- 101000878906 Bacillus thuringiensis Pesticidal crystal protein Cry6Ba Proteins 0.000 description 9
- 239000002253 acid Substances 0.000 description 9
- 239000002671 adjuvant Substances 0.000 description 9
- 230000008827 biological function Effects 0.000 description 9
- 235000013305 food Nutrition 0.000 description 9
- 238000006062 fragmentation reaction Methods 0.000 description 9
- 230000012010 growth Effects 0.000 description 9
- 230000001404 mediated effect Effects 0.000 description 9
- 239000000843 powder Substances 0.000 description 9
- 108091008146 restriction endonucleases Proteins 0.000 description 9
- 230000001131 transforming effect Effects 0.000 description 9
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 8
- 206010035226 Plasma cell myeloma Diseases 0.000 description 8
- 125000000539 amino acid group Chemical group 0.000 description 8
- 230000027455 binding Effects 0.000 description 8
- 238000013016 damping Methods 0.000 description 8
- 230000034994 death Effects 0.000 description 8
- 239000000428 dust Substances 0.000 description 8
- 239000012530 fluid Substances 0.000 description 8
- 238000013467 fragmentation Methods 0.000 description 8
- 210000001161 mammalian embryo Anatomy 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 201000000050 myeloid neoplasm Diseases 0.000 description 8
- 239000007787 solid Substances 0.000 description 8
- 230000009466 transformation Effects 0.000 description 8
- 241000589158 Agrobacterium Species 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- 108020004511 Recombinant DNA Proteins 0.000 description 7
- 230000000890 antigenic effect Effects 0.000 description 7
- 238000013459 approach Methods 0.000 description 7
- 230000002163 immunogen Effects 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 230000035772 mutation Effects 0.000 description 7
- 230000008521 reorganization Effects 0.000 description 7
- 238000000926 separation method Methods 0.000 description 7
- 238000002741 site-directed mutagenesis Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000012546 transfer Methods 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 241000282461 Canis lupus Species 0.000 description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 6
- 239000000020 Nitrocellulose Substances 0.000 description 6
- 101710141454 Nucleoprotein Proteins 0.000 description 6
- 230000003115 biocidal effect Effects 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 6
- 230000009089 cytolysis Effects 0.000 description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 6
- 230000004927 fusion Effects 0.000 description 6
- 239000007924 injection Substances 0.000 description 6
- 229930182817 methionine Natural products 0.000 description 6
- 238000002703 mutagenesis Methods 0.000 description 6
- 231100000350 mutagenesis Toxicity 0.000 description 6
- 229920001220 nitrocellulos Polymers 0.000 description 6
- 239000002953 phosphate buffered saline Substances 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 210000001938 protoplast Anatomy 0.000 description 6
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 5
- 108700003918 Bacillus Thuringiensis insecticidal crystal Proteins 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- 241000701489 Cauliflower mosaic virus Species 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 5
- 206010020649 Hyperkeratosis Diseases 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 5
- 150000007513 acids Chemical class 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 5
- 229940098773 bovine serum albumin Drugs 0.000 description 5
- 239000000969 carrier Substances 0.000 description 5
- 230000002209 hydrophobic effect Effects 0.000 description 5
- 238000002347 injection Methods 0.000 description 5
- 238000005457 optimization Methods 0.000 description 5
- 229940049547 paraxin Drugs 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 239000006152 selective media Substances 0.000 description 5
- 210000000952 spleen Anatomy 0.000 description 5
- 239000000758 substrate Substances 0.000 description 5
- 238000011144 upstream manufacturing Methods 0.000 description 5
- NWXMGUDVXFXRIG-WESIUVDSSA-N (4s,4as,5as,6s,12ar)-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]4(O)C(=O)C3=C(O)C2=C1O NWXMGUDVXFXRIG-WESIUVDSSA-N 0.000 description 4
- 101710106459 29 kDa protein Proteins 0.000 description 4
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 4
- 108091029865 Exogenous DNA Proteins 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 108091034057 RNA (poly(A)) Proteins 0.000 description 4
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 230000002362 anti-crystal effect Effects 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 4
- 210000002421 cell wall Anatomy 0.000 description 4
- 238000012937 correction Methods 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 238000009313 farming Methods 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 230000036571 hydration Effects 0.000 description 4
- 238000006703 hydration reaction Methods 0.000 description 4
- 230000005847 immunogenicity Effects 0.000 description 4
- 235000009973 maize Nutrition 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000013011 mating Effects 0.000 description 4
- 238000007500 overflow downdraw method Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 230000001568 sexual effect Effects 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 238000001262 western blot Methods 0.000 description 4
- AGNGYMCLFWQVGX-AGFFZDDWSA-N (e)-1-[(2s)-2-amino-2-carboxyethoxy]-2-diazonioethenolate Chemical compound OC(=O)[C@@H](N)CO\C([O-])=C\[N+]#N AGNGYMCLFWQVGX-AGFFZDDWSA-N 0.000 description 3
- TVZGACDUOSZQKY-LBPRGKRZSA-N 4-aminofolic acid Chemical compound C1=NC2=NC(N)=NC(N)=C2N=C1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 TVZGACDUOSZQKY-LBPRGKRZSA-N 0.000 description 3
- 239000004475 Arginine Substances 0.000 description 3
- 108090001008 Avidin Proteins 0.000 description 3
- 108020004705 Codon Proteins 0.000 description 3
- 241000255925 Diptera Species 0.000 description 3
- 108091027305 Heteroduplex Proteins 0.000 description 3
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 3
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 3
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 3
- 102000003960 Ligases Human genes 0.000 description 3
- 108090000364 Ligases Proteins 0.000 description 3
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 description 3
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 3
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 3
- 239000004473 Threonine Substances 0.000 description 3
- 240000003834 Triticum spelta Species 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- JUGOREOARAHOCO-UHFFFAOYSA-M acetylcholine chloride Chemical compound [Cl-].CC(=O)OCC[N+](C)(C)C JUGOREOARAHOCO-UHFFFAOYSA-M 0.000 description 3
- 229960003896 aminopterin Drugs 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 3
- 235000003704 aspartic acid Nutrition 0.000 description 3
- 229950011321 azaserine Drugs 0.000 description 3
- 210000003719 b-lymphocyte Anatomy 0.000 description 3
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 238000004422 calculation algorithm Methods 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 101150086784 cry gene Proteins 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 210000003495 flagella Anatomy 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 229960002989 glutamic acid Drugs 0.000 description 3
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 238000001114 immunoprecipitation Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 229960000310 isoleucine Drugs 0.000 description 3
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 3
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 108020004999 messenger RNA Proteins 0.000 description 3
- 229960000485 methotrexate Drugs 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 239000002853 nucleic acid probe Substances 0.000 description 3
- 102000013415 peroxidase activity proteins Human genes 0.000 description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 description 3
- 108091033319 polynucleotide Proteins 0.000 description 3
- 102000040430 polynucleotide Human genes 0.000 description 3
- 239000002157 polynucleotide Substances 0.000 description 3
- 238000003127 radioimmunoassay Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 208000011580 syndromic disease Diseases 0.000 description 3
- 231100000765 toxin Toxicity 0.000 description 3
- 230000005030 transcription termination Effects 0.000 description 3
- 238000011426 transformation method Methods 0.000 description 3
- 238000009736 wetting Methods 0.000 description 3
- AUHDWARTFSKSAC-HEIFUQTGSA-N (2S,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)-2-(6-oxo-1H-purin-9-yl)oxolane-2-carboxylic acid Chemical group [C@]1([C@H](O)[C@H](O)[C@@H](CO)O1)(N1C=NC=2C(O)=NC=NC12)C(=O)O AUHDWARTFSKSAC-HEIFUQTGSA-N 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- 108010088751 Albumins Proteins 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 244000003416 Asparagus officinalis Species 0.000 description 2
- 235000005340 Asparagus officinalis Nutrition 0.000 description 2
- 241001147758 Bacillus thuringiensis serovar kurstaki Species 0.000 description 2
- 241000178575 Bacillus thuringiensis serovar thompsoni Species 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 description 2
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 108010025815 Kanamycin Kinase Proteins 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- 108090001090 Lectins Proteins 0.000 description 2
- 102000004856 Lectins Human genes 0.000 description 2
- 241000258916 Leptinotarsa decemlineata Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 108091000080 Phosphotransferase Proteins 0.000 description 2
- 241000276498 Pollachius virens Species 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 2
- 241001620634 Roger Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- CWHJIJJSDGEHNS-MYLFLSLOSA-N Senegenin Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(C(O)=O)[C@@H]2CC[C@@]3(C)C(CC[C@]4(CCC(C[C@H]44)(C)C)C(O)=O)=C4[C@@H](CCl)C[C@@H]3[C@]21C CWHJIJJSDGEHNS-MYLFLSLOSA-N 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 239000006035 Tryptophane Substances 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 235000007244 Zea mays Nutrition 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000000735 allogeneic effect Effects 0.000 description 2
- 210000000628 antibody-producing cell Anatomy 0.000 description 2
- 210000001106 artificial yeast chromosome Anatomy 0.000 description 2
- 229960001230 asparagine Drugs 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000001506 calcium phosphate Substances 0.000 description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 description 2
- 235000011010 calcium phosphates Nutrition 0.000 description 2
- 230000010307 cell transformation Effects 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000003593 chromogenic compound Substances 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 101150102059 cry3Aa gene Proteins 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000009795 derivation Methods 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000014103 egg white Nutrition 0.000 description 2
- 210000000969 egg white Anatomy 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 241001233957 eudicotyledons Species 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 244000037666 field crops Species 0.000 description 2
- 238000001502 gel electrophoresis Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 2
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
- 229920001519 homopolymer Polymers 0.000 description 2
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 2
- 238000003119 immunoblot Methods 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 235000013902 inosinic acid Nutrition 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000002523 lectin Substances 0.000 description 2
- 239000008176 lyophilized powder Substances 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 230000009871 nonspecific binding Effects 0.000 description 2
- 239000006916 nutrient agar Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 239000002751 oligonucleotide probe Substances 0.000 description 2
- 230000000590 parasiticidal effect Effects 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 239000001814 pectin Substances 0.000 description 2
- 229920001277 pectin Polymers 0.000 description 2
- 235000010987 pectin Nutrition 0.000 description 2
- 210000005259 peripheral blood Anatomy 0.000 description 2
- 239000011886 peripheral blood Substances 0.000 description 2
- 230000002085 persistent effect Effects 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 102000020233 phosphotransferase Human genes 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 230000007096 poisonous effect Effects 0.000 description 2
- -1 polyoxyethylene Polymers 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 230000006916 protein interaction Effects 0.000 description 2
- 238000012207 quantitative assay Methods 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 102220023258 rs387907548 Human genes 0.000 description 2
- DAJSVUQLFFJUSX-UHFFFAOYSA-M sodium;dodecane-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCS([O-])(=O)=O DAJSVUQLFFJUSX-UHFFFAOYSA-M 0.000 description 2
- 239000008279 sol Substances 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000004094 surface-active agent Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 239000009871 tenuigenin Substances 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- 230000010474 transient expression Effects 0.000 description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
- 229960004799 tryptophan Drugs 0.000 description 2
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 2
- 239000004563 wettable powder Substances 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- YRSDVHGZBOKELJ-DHXGRXBNSA-N (2s,3r,4s,5s,6r)-2-[2-(3,4-dihydroxy-5-methoxyphenyl)-7-hydroxy-3-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromenylium-5-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol;chloride Chemical compound [Cl-].OC1=C(O)C(OC)=CC(C=2C(=CC=3C(O[C@H]4[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O)=CC(O)=CC=3[O+]=2)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)=C1 YRSDVHGZBOKELJ-DHXGRXBNSA-N 0.000 description 1
- LKKMLIBUAXYLOY-UHFFFAOYSA-N 3-Amino-1-methyl-5H-pyrido[4,3-b]indole Chemical compound N1C2=CC=CC=C2C2=C1C=C(N)N=C2C LKKMLIBUAXYLOY-UHFFFAOYSA-N 0.000 description 1
- LPXQRXLUHJKZIE-UHFFFAOYSA-N 8-azaguanine Chemical compound NC1=NC(O)=C2NN=NC2=N1 LPXQRXLUHJKZIE-UHFFFAOYSA-N 0.000 description 1
- 229960005508 8-azaguanine Drugs 0.000 description 1
- 241000902874 Agelastica alni Species 0.000 description 1
- 101710186708 Agglutinin Proteins 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- 101710187578 Alcohol dehydrogenase 1 Proteins 0.000 description 1
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 description 1
- KHOITXIGCFIULA-UHFFFAOYSA-N Alophen Chemical compound C1=CC(OC(=O)C)=CC=C1C(C=1N=CC=CC=1)C1=CC=C(OC(C)=O)C=C1 KHOITXIGCFIULA-UHFFFAOYSA-N 0.000 description 1
- 241000254177 Anthonomus Species 0.000 description 1
- 241001566735 Archon Species 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 241000209763 Avena sativa Species 0.000 description 1
- 235000007558 Avena sp Nutrition 0.000 description 1
- 238000011725 BALB/c mouse Methods 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 101000941928 Bacillus thuringiensis Pesticidal crystal protein Cry2Ac Proteins 0.000 description 1
- 241000193367 Bacillus thuringiensis serovar san diego Species 0.000 description 1
- 101100061321 Bacillus thuringiensis subsp. japonensis cry8Ca gene Proteins 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- 241000219495 Betulaceae Species 0.000 description 1
- 241000908115 Bolivar Species 0.000 description 1
- 241000193417 Brevibacillus laterosporus Species 0.000 description 1
- 210000003771 C cell Anatomy 0.000 description 1
- 101100015199 Caenorhabditis elegans gly-11 gene Proteins 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 206010057248 Cell death Diseases 0.000 description 1
- 108010004539 Chalcone isomerase Proteins 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 241000254171 Curculionidae Species 0.000 description 1
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 102100033072 DNA replication ATP-dependent helicase DNA2 Human genes 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 1
- 108010047524 Deoxyribonuclease HindIII Proteins 0.000 description 1
- 241000489973 Diabrotica undecimpunctata Species 0.000 description 1
- 241000489976 Diabrotica undecimpunctata howardi Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 240000004859 Gamochaeta purpurea Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 1
- 101000927313 Homo sapiens DNA replication ATP-dependent helicase DNA2 Proteins 0.000 description 1
- 101710146024 Horcolin Proteins 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 241001135569 Human adenovirus 5 Species 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- GRSZFWQUAKGDAV-UHFFFAOYSA-N Inosinic acid Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(NC=NC2=O)=C2N=C1 GRSZFWQUAKGDAV-UHFFFAOYSA-N 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
- 240000007049 Juglans regia Species 0.000 description 1
- 235000009496 Juglans regia Nutrition 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 1
- 101710189395 Lectin Proteins 0.000 description 1
- 241000314941 Leptinotarsa texana Species 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- VPRLICVDSGMIKO-UHFFFAOYSA-N Mannopine Natural products NC(=O)CCC(C(O)=O)NCC(O)C(O)C(O)C(O)CO VPRLICVDSGMIKO-UHFFFAOYSA-N 0.000 description 1
- 101710179758 Mannose-specific lectin Proteins 0.000 description 1
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 description 1
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 101710164418 Movement protein TGB2 Proteins 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 241000711408 Murine respirovirus Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 108010064696 N,O-diacetylmuramidase Proteins 0.000 description 1
- 101100285000 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) his-3 gene Proteins 0.000 description 1
- 101100426589 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) trp-3 gene Proteins 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- ALKWJXWZUTYERW-UHFFFAOYSA-L O.O.O.O.[Mn](=O)(Cl)Cl Chemical class O.O.O.O.[Mn](=O)(Cl)Cl ALKWJXWZUTYERW-UHFFFAOYSA-L 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 101710091688 Patatin Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- KLRABYJGMPNMSA-ZUUMSKAQSA-O Petunin Natural products OC1=C(O)C(OC)=CC(C=2C(=CC=3C(O[C@H]4[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O)=CC(O)=CC=3[O+]=2)O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)=C1 KLRABYJGMPNMSA-ZUUMSKAQSA-O 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 241000254103 Popillia Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 102100021225 Serine hydroxymethyltransferase, cytosolic Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 101710156615 Sucrose synthase 1 Proteins 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 241000254105 Tenebrio Species 0.000 description 1
- 241000218636 Thuja Species 0.000 description 1
- 102400000336 Thyrotropin-releasing hormone Human genes 0.000 description 1
- 241000173347 Tonsilla Species 0.000 description 1
- 241000254086 Tribolium <beetle> Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 229930003756 Vitamin B7 Natural products 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 241001143315 Xanthogaleruca luteola Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- ZKHQWZAMYRWXGA-KNYAHOBESA-N [[(2r,3s,4r,5r)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] dihydroxyphosphoryl hydrogen phosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)O[32P](O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KNYAHOBESA-N 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 101150063416 add gene Proteins 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 230000002788 anti-peptide Effects 0.000 description 1
- 101150036080 at gene Proteins 0.000 description 1
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 1
- 102000055574 bcl-2 Homologous Antagonist-Killer Human genes 0.000 description 1
- 108700039689 bcl-2 Homologous Antagonist-Killer Proteins 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical group C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- NDTSRXAMMQDVSW-UHFFFAOYSA-N benzthiazide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(S(N2)(=O)=O)=C1N=C2CSCC1=CC=CC=C1 NDTSRXAMMQDVSW-UHFFFAOYSA-N 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000003181 biological factor Substances 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 238000000071 blow moulding Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 102220369447 c.1352G>A Human genes 0.000 description 1
- 102220369445 c.668T>C Human genes 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 239000013592 cell lysate Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000003283 colorimetric indicator Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 101150085721 cry2Aa gene Proteins 0.000 description 1
- 230000024835 cytogamy Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 230000009365 direct transmission Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000010410 dusting Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 239000004495 emulsifiable concentrate Substances 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000008175 fetal development Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 108010074605 gamma-Globulins Proteins 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 108060003552 hemocyanin Proteins 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 231100000652 hormesis Toxicity 0.000 description 1
- 230000007236 host immunity Effects 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 210000004754 hybrid cell Anatomy 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 239000004245 inosinic acid Substances 0.000 description 1
- 229940028843 inosinic acid Drugs 0.000 description 1
- 230000016507 interphase Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 238000007912 intraperitoneal administration Methods 0.000 description 1
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 description 1
- 229910000360 iron(III) sulfate Inorganic materials 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- XUWPJKDMEZSVTP-LTYMHZPRSA-N kalafungina Chemical compound O=C1C2=C(O)C=CC=C2C(=O)C2=C1[C@@H](C)O[C@H]1[C@@H]2OC(=O)C1 XUWPJKDMEZSVTP-LTYMHZPRSA-N 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 210000003712 lysosome Anatomy 0.000 description 1
- 230000001868 lysosomic effect Effects 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- VPRLICVDSGMIKO-SZWOQXJISA-N mannopine Chemical compound NC(=O)CC[C@@H](C(O)=O)NC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO VPRLICVDSGMIKO-SZWOQXJISA-N 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- 210000000865 mononuclear phagocyte system Anatomy 0.000 description 1
- 239000002088 nanocapsule Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 230000004145 nucleotide salvage Effects 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000014571 nuts Nutrition 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000013618 particulate matter Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 210000004976 peripheral blood cell Anatomy 0.000 description 1
- 210000003200 peritoneal cavity Anatomy 0.000 description 1
- 230000000361 pesticidal effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 210000003720 plasmablast Anatomy 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 229920002796 poly[α-(4-aminobutyl)-L-glycolic acid) Polymers 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000012716 precipitator Substances 0.000 description 1
- 229940052586 pro 12 Drugs 0.000 description 1
- 230000001141 propulsive effect Effects 0.000 description 1
- 238000001814 protein method Methods 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000000518 rheometry Methods 0.000 description 1
- 102220023257 rs387907546 Human genes 0.000 description 1
- 102220023256 rs387907547 Human genes 0.000 description 1
- 238000009991 scouring Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 230000035040 seed growth Effects 0.000 description 1
- 230000010153 self-pollination Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- HBMJWWWQQXIZIP-UHFFFAOYSA-N silicon carbide Chemical compound [Si+]#[C-] HBMJWWWQQXIZIP-UHFFFAOYSA-N 0.000 description 1
- 229910010271 silicon carbide Inorganic materials 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000012868 site-directed mutagenesis technique Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000003393 splenic effect Effects 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000003656 tris buffered saline Substances 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 238000001429 visible spectrum Methods 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
- 235000011912 vitamin B7 Nutrition 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 230000007279 water homeostasis Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
- C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)
- G01N2333/325—Bacillus thuringiensis crystal protein (delta-endotoxin)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/82—Proteins from microorganisms
- Y10S530/825—Bacteria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (41)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/718,905 US6063756A (en) | 1996-09-24 | 1996-09-24 | Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor |
US08/718,905 | 1996-09-24 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1241213A CN1241213A (zh) | 2000-01-12 |
CN100473725C true CN100473725C (zh) | 2009-04-01 |
Family
ID=24888036
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB971800022A Expired - Lifetime CN100473725C (zh) | 1996-09-24 | 1997-09-24 | 苏云金芽孢杆菌CryET33和CryET34组合物及其使用 |
Country Status (19)
Country | Link |
---|---|
US (8) | US6063756A (zh) |
EP (1) | EP1015592B1 (zh) |
JP (1) | JP2001523944A (zh) |
KR (1) | KR20000048593A (zh) |
CN (1) | CN100473725C (zh) |
AR (2) | AR010993A1 (zh) |
AT (1) | ATE324447T1 (zh) |
AU (1) | AU741704B2 (zh) |
BR (1) | BR9713219B1 (zh) |
CA (1) | CA2267665C (zh) |
CO (1) | CO4650229A1 (zh) |
DE (1) | DE69735776T2 (zh) |
IL (1) | IL129160A0 (zh) |
MX (1) | MXPA99002819A (zh) |
PL (1) | PL189474B1 (zh) |
TR (1) | TR199900650T2 (zh) |
UA (1) | UA75317C2 (zh) |
UY (1) | UY24725A1 (zh) |
WO (1) | WO1998013498A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107074974A (zh) * | 2014-10-16 | 2017-08-18 | 孟山都技术有限公司 | 对鳞翅目害虫具有毒性或抑制性的新型嵌合杀昆虫蛋白质 |
Families Citing this family (124)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6063756A (en) * | 1996-09-24 | 2000-05-16 | Monsanto Company | Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor |
US6365129B1 (en) | 1999-08-04 | 2002-04-02 | Tosk, Inc. | Invivo high throughput toxicology screening method |
DK1206567T3 (da) * | 1999-08-04 | 2009-05-11 | Tosk Inc | In vivo höj-kapacitet toksikologisk screeningsfremgangsmåde |
AU2001290919A1 (en) | 2000-09-12 | 2002-03-26 | Monsanto Technology Llc | Insect inhibitory bacillus thuringiensis proteins, fusions, and methods of use therefor |
US9816104B2 (en) * | 2000-10-06 | 2017-11-14 | Monsanto Technology Llc | Compositions and methods for deploying a transgenic refuge as a seed blend |
EP1551966B1 (en) | 2002-07-18 | 2013-06-05 | Monsanto Technology LLC | Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing |
US20050033137A1 (en) * | 2002-10-25 | 2005-02-10 | The Regents Of The University Of Michigan | Ablation catheters and methods for their use |
BRPI0408896A (pt) | 2003-03-28 | 2006-04-25 | Monsanto Technology Llc | promotores de planta para uso no desenvolvimento precoce de sementes |
WO2005050216A1 (en) | 2003-07-25 | 2005-06-02 | The University Of Georgia Research Foundation, Inc. | Identification of toxin-binding protein involved in resistance to cry1 toxins, and related screening methods |
EP2343364B1 (en) | 2003-08-25 | 2016-10-05 | Monsanto Technology LLC | Tubulin regulatory elements for use in plants |
EP1708561B1 (en) | 2003-12-16 | 2010-11-10 | Monsanto Technology LLC | Secreted insecticidal protein and gene compositions from bacillus thuringiensis and uses therefor |
WO2005069986A2 (en) | 2004-01-20 | 2005-08-04 | Monsanto Technology Llc | Chimeric promoters for use in plants |
WO2006023869A2 (en) | 2004-08-24 | 2006-03-02 | Monsanto Technology Llc | Adenylate translocator protein gene non-coding regulatory elements for use in plants |
AU2005285014A1 (en) | 2004-09-14 | 2006-03-23 | Monsanto Technology Llc | Promoter molecules for use in plants |
WO2006089950A2 (en) | 2005-02-26 | 2006-08-31 | Basf Plant Science Gmbh | Expression cassettes for seed-preferential expression in plants |
EP1874938B1 (en) | 2005-04-19 | 2012-04-04 | BASF Plant Science GmbH | Starchy-endosperm and/or germinating embryo-specific expression in mono-cotyledonous plants |
US7790873B2 (en) | 2005-05-10 | 2010-09-07 | Basf Plant Science Gmbh | Expression cassettes for seed-preferential expression in plants |
US8993846B2 (en) | 2005-09-06 | 2015-03-31 | Monsanto Technology Llc | Vectors and methods for improved plant transformation efficiency |
EP2843053A1 (en) | 2006-02-17 | 2015-03-04 | Monsanto Technology LLC | Chimeric regulatory sequences comprising introns for plant gene expression |
EP2455392A3 (en) | 2006-06-15 | 2012-08-22 | Athenix Corporation | A family of pesticidal proteins and methods for their use |
WO2008099013A1 (en) | 2007-02-16 | 2008-08-21 | Basf Plant Science Gmbh | Nucleic acid sequences for regulation of embryo-specific expression in monocotyledonous plants |
US7838729B2 (en) | 2007-02-26 | 2010-11-23 | Monsanto Technology Llc | Chloroplast transit peptides for efficient targeting of DMO and uses thereof |
US10036036B1 (en) * | 2007-03-15 | 2018-07-31 | Monsanto Technology Llc | Compositions and methods for deploying a transgenic refuge as a seed blend |
MX2010005352A (es) * | 2007-11-15 | 2010-07-02 | Monsanto Technology Llc | Plantas y semillas de soya correspondientes al evento transgenico mon87701 y metodos de deteccion del mismo. |
CA3086659A1 (en) | 2007-12-26 | 2009-07-09 | Xencor, Inc. | Fc variants with altered binding to fcrn |
SI2262896T1 (sl) | 2008-04-07 | 2014-05-30 | Monsanto Technology Llc | Rastlinski regulatorni elementi in njihove uporabe |
WO2010099431A2 (en) | 2009-02-27 | 2010-09-02 | Monsanto Technology Llc | Hydroponic apparatus and methods of use |
US9980423B2 (en) | 2009-05-03 | 2018-05-29 | Monsanto Technology Llc | Systems and processes for combining different types of seeds |
CA2766858A1 (en) | 2009-07-10 | 2011-01-13 | Basf Plant Science Company Gmbh | Expression cassettes for endosperm-specific expression in plants |
US8937214B2 (en) | 2009-10-23 | 2015-01-20 | Monsanto Technology Llc | Methods and compositions for expression of transgenes in plants |
EP2507375A4 (en) | 2009-12-03 | 2013-04-24 | Basf Plant Science Co Gmbh | EXPRESSION CASSETTES FOR EMBRYOSPECIFIC EXPRESSION IN PLANTS |
CA2786697C (en) | 2010-01-14 | 2018-10-30 | Stanislaw Flasinski | Plant regulatory elements and uses thereof |
DK2611925T3 (en) | 2010-08-30 | 2018-02-26 | Dow Agrosciences Llc | AMPLIFIER ELEMENTS OF BACCILE-SUGAR PIPE VIRUS (SCBV) AND ITS USE IN FUNCTIONAL RESEARCH IN PLANTS |
CA2825951C (en) * | 2011-02-11 | 2019-08-20 | Monsanto Technology Llc | Pesticidal nucleic acids and proteins and uses thereof |
AU2012237662B2 (en) | 2011-03-25 | 2017-06-15 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
EA031839B1 (ru) | 2011-05-13 | 2019-02-28 | Монсанто Текнолоджи Ллс | Регуляторные элементы растений и их применение |
CN103125516B (zh) * | 2011-11-24 | 2014-12-17 | 华中农业大学 | 对南方根结线虫具有杀虫增效作用的蛋白组合物Cry6Aa/Cry55Aa及应用 |
KR20140130506A (ko) | 2012-02-29 | 2014-11-10 | 다우 아그로사이언시즈 엘엘씨 | 사탕수수 바실루스형 바이러스 (scbv) 인핸서 및 식물 기능성 유전체학에서의 이의 용도 |
KR20220047395A (ko) | 2012-03-09 | 2022-04-15 | 베스타론 코포레이션 | 독성 펩타이드 제조, 식물에서의 펩타이드 발현 및 시스테인 농후 펩타이드의 조합 |
US11692016B2 (en) | 2012-03-09 | 2023-07-04 | Vestaron Corporation | High gene expression yeast strain |
US9663793B2 (en) | 2012-04-20 | 2017-05-30 | Monsanto Technology, Llc | Plant regulatory elements and uses thereof |
US20150211019A1 (en) | 2012-08-13 | 2015-07-30 | University Of Georgia Research Foundation, Inc. | Compositions and Methods for Increasing Pest Resistance in Plants |
CA2887575A1 (en) | 2012-10-15 | 2014-04-24 | Pioneer Hi-Bred International, Inc. | Methods and compositions to enhance activity of cry endotoxins |
WO2014071182A1 (en) | 2012-11-01 | 2014-05-08 | Massachusetts Institute Of Technology | Directed evolution of synthetic gene cluster |
CN104995301B (zh) | 2012-12-19 | 2018-07-20 | 孟山都技术公司 | 植物调控元件及其用途 |
WO2014153254A2 (en) | 2013-03-14 | 2014-09-25 | Pioneer Hi-Bred International Inc. | Compositions and methods to control insect pests |
EP3613279A1 (en) | 2013-03-14 | 2020-02-26 | Monsanto Technology LLC | Plant regulatory elements and uses thereof |
KR102270069B1 (ko) | 2013-03-14 | 2021-06-28 | 몬산토 테크놀로지 엘엘씨 | 식물 조절 성분 및 이의 용도 |
US10023877B2 (en) | 2013-03-15 | 2018-07-17 | Pioneer Hi-Bred International, Inc. | PHI-4 polypeptides and methods for their use |
US9365863B2 (en) | 2013-05-08 | 2016-06-14 | Monsanto Technology Llc | Compositions and methods for deploying a transgenic refuge seed blend |
US9974302B2 (en) * | 2013-08-09 | 2018-05-22 | Monsanto Technology Llc | Pesticidal toxin active against coleopteran and/or hemipteran insects |
MX359026B (es) | 2013-08-16 | 2018-09-12 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos de uso. |
MX359027B (es) | 2013-09-13 | 2018-09-12 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
CN103570811B (zh) * | 2013-11-19 | 2015-06-03 | 中国农业科学院植物保护研究所 | 苏云金芽孢杆菌基因cry1Ah3及其应用 |
CN106232620B (zh) | 2014-02-07 | 2022-05-13 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
MX2016010187A (es) | 2014-02-07 | 2017-07-11 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
WO2016000237A1 (en) | 2014-07-03 | 2016-01-07 | Pioneer Overseas Corporation | Plants having enhanced tolerance to insect pests and related constructs and methods involving insect tolerance genes |
US20170247719A1 (en) | 2014-09-17 | 2017-08-31 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
CN113372421A (zh) | 2014-10-16 | 2021-09-10 | 先锋国际良种公司 | 杀昆虫蛋白及其使用方法 |
WO2016106066A2 (en) | 2014-12-22 | 2016-06-30 | AgBiome, Inc. | Pesticidal genes and methods of use |
EA038923B1 (ru) | 2015-03-11 | 2021-11-10 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидная днк-конструкция и способы её применения |
WO2016154631A1 (en) | 2015-03-26 | 2016-09-29 | The Texas A&M University System | Conversion of lignin into bioplastics and lipid fuels |
WO2016168289A1 (en) | 2015-04-17 | 2016-10-20 | AgBiome, Inc. | Pesticidal genes and methods of use |
MA41951A (fr) | 2015-04-22 | 2018-02-28 | Agbiome Inc | Gènes insecticides et procédés d'utilisation |
RU2017144238A (ru) | 2015-05-19 | 2019-06-19 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
CN108271390A (zh) | 2015-06-03 | 2018-07-10 | 农业生物群落股份有限公司 | 杀虫基因和使用方法 |
EP3310803A1 (en) | 2015-06-16 | 2018-04-25 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
AU2016283985B2 (en) | 2015-06-22 | 2021-01-21 | AgBiome, Inc. | Pesticidal genes and methods of use |
WO2017011602A1 (en) | 2015-07-13 | 2017-01-19 | Pivot Bio, Inc. | Methods and compositions for improving plant traits |
EP3943602A1 (en) | 2015-08-06 | 2022-01-26 | Pioneer Hi-Bred International, Inc. | Plant derived insecticidal proteins and methods for their use |
BR112018004108A2 (pt) | 2015-08-28 | 2018-12-11 | Du Pont | ochrobactrum haywardense h1 isolada, ochrobactrum haywardense h1, método para produzir uma célula vegetal transformada, kit e vetor |
WO2017062412A1 (en) | 2015-10-05 | 2017-04-13 | Massachusetts Institute Of Technology | Nitrogen fixation using refactored nif clusters |
EP3390431A1 (en) | 2015-12-18 | 2018-10-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
RU2021110857A (ru) | 2015-12-22 | 2021-04-26 | Агбайоми, Инк. | Пестицидные гены и способы использования |
US10443066B2 (en) | 2016-03-11 | 2019-10-15 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
US11781151B2 (en) | 2016-04-14 | 2023-10-10 | Pioneer Hi-Bred International, Inc. | Insecticidal CRY1B variants having improved activity spectrum and uses thereof |
WO2017184673A1 (en) | 2016-04-19 | 2017-10-26 | Pioneer Hi-Bred International, Inc. | Insecticidal combinations of polypeptides having improved activity spectrum and uses thereof |
CA3018384A1 (en) | 2016-05-04 | 2017-11-09 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CA3024452A1 (en) | 2016-05-24 | 2017-11-30 | Monsanto Technology Llc | Plant regulatory elements and uses thereof |
EP3472323A1 (en) | 2016-06-16 | 2019-04-24 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
CN109642237B (zh) | 2016-06-24 | 2022-09-30 | 先锋国际良种公司 | 植物调节元件及其使用方法 |
WO2018005589A1 (en) | 2016-06-28 | 2018-01-04 | Cellectis | Altering expression of gene products in plants through targeted insertion of nucleic acid sequences |
CA3026113A1 (en) | 2016-07-01 | 2018-01-04 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
WO2018013333A1 (en) | 2016-07-12 | 2018-01-18 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
CN110214189A (zh) | 2016-09-06 | 2019-09-06 | 农业生物群落股份有限公司 | 杀虫基因和使用方法 |
MX2019004648A (es) | 2016-10-21 | 2019-05-23 | Vestaron Corp | Peptidos escindibles y proteinas insecticidas y nematicidas que los comprenden. |
EP4050021A1 (en) | 2016-11-01 | 2022-08-31 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CA3044404A1 (en) | 2016-12-14 | 2018-06-21 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CA3046226A1 (en) | 2016-12-22 | 2018-06-28 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
UY37566A (es) | 2017-01-12 | 2018-07-31 | Pivot Bio Inc | Métodos y composiciones para mejorar características en plantas |
WO2018140214A1 (en) | 2017-01-24 | 2018-08-02 | Pioneer Hi-Bred International, Inc. | Nematicidal protein from pseudomonas |
WO2018140859A2 (en) | 2017-01-30 | 2018-08-02 | AgBiome, Inc. | Pesticidal genes and methods of use |
WO2018148001A1 (en) | 2017-02-08 | 2018-08-16 | Pioneer Hi-Bred International Inc | Insecticidal combinations of plant derived insecticidal proteins and methods for their use |
BR112019021380A2 (pt) | 2017-04-11 | 2020-05-05 | Agbiome Inc | genes pesticidas e métodos de uso |
EA201992824A1 (ru) | 2017-05-26 | 2020-05-06 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные полипептиды, обладающие улучшенным спектром активности, и пути их применения |
BR112020002279A2 (pt) | 2017-08-03 | 2020-07-28 | AgBiome, Inc. | genes pesticidas e métodos de uso |
US20200165626A1 (en) | 2017-10-13 | 2020-05-28 | Pioneer Hi-Bred International, Inc. | Virus-induced gene silencing technology for insect control in maize |
KR20200088342A (ko) | 2017-10-25 | 2020-07-22 | 피벗 바이오, 인크. | 질소를 고정하는 유전자조작 미생물을 개선하는 방법 및 조성물 |
EP3728606A1 (en) | 2017-12-22 | 2020-10-28 | Agbiome, Inc. | Pesticidal genes and methods of use |
EP3737747A1 (en) | 2018-01-12 | 2020-11-18 | The Texas A&M University System | Increasing plant bioproduct yield |
WO2019165245A1 (en) | 2018-02-22 | 2019-08-29 | Zymergen Inc. | Method for creating a genomic library enriched for bacillus and identification of novel cry toxins |
US20210002657A1 (en) | 2018-03-02 | 2021-01-07 | Pioneer Hi-Bred International, Inc. | Plant health assay |
CA3088011A1 (en) | 2018-03-02 | 2019-09-06 | Zymergen Inc. | Insecticidal protein discovery platform and insecticidal proteins discovered therefrom |
CA3097621A1 (en) | 2018-04-20 | 2019-10-24 | AgBiome, Inc. | Pesticidal proteins and methods of use |
WO2019226508A1 (en) | 2018-05-22 | 2019-11-28 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
MX2020013875A (es) | 2018-06-27 | 2021-08-11 | Pivot Bio Inc | Composiciones agricolas que comprenden microbios remodelados de fijacion de nitrogeno. |
MX2021002290A (es) | 2018-08-29 | 2021-04-28 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para su uso. |
WO2021076346A1 (en) | 2019-10-18 | 2021-04-22 | Pioneer Hi-Bred International, Inc. | Maize event dp-202216-6 and dp-023211-2 stack |
TW202142114A (zh) | 2020-02-04 | 2021-11-16 | 美商陶氏農業科學公司 | 具有殺有害生物效用之組成物及與其相關之方法 |
CA3186978A1 (en) | 2020-07-14 | 2022-01-20 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CA3189603A1 (en) | 2020-08-10 | 2022-01-17 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
US11780890B2 (en) | 2020-11-24 | 2023-10-10 | AgBiome, Inc. | Pesticidal genes and methods of use |
AR125217A1 (es) | 2021-03-26 | 2023-06-28 | Flagship Pioneering Innovations Vii Llc | Producción de polirribonucleótidos circulares en un sistema procariota |
EP4314289A1 (en) | 2021-03-26 | 2024-02-07 | Flagship Pioneering Innovations VII, LLC | Production of circular polyribonucleotides in a prokaryotic system |
TW202300650A (zh) | 2021-03-26 | 2023-01-01 | 美商旗艦先鋒創新有限責任(Vii)公司 | 真核系統中環狀多核糖核苷酸的產生 |
CA3214227A1 (en) | 2021-05-06 | 2022-11-10 | Rebekah Deter Kelly | Pesticidal genes and methods of use |
AU2022375817A1 (en) | 2021-11-01 | 2024-05-09 | Flagship Pioneering Innovations Vii, Llc | Polynucleotides for modifying organisms |
WO2023107943A1 (en) | 2021-12-07 | 2023-06-15 | AgBiome, Inc. | Pesticidal genes and methods of use |
WO2023141540A2 (en) | 2022-01-20 | 2023-07-27 | Flagship Pioneering Innovations Vii, Llc | Polynucleotides for modifying organisms |
CN114672444B (zh) * | 2022-05-13 | 2023-03-10 | 福州大学 | 一株苏云金芽孢杆菌及其在不饱和烯烃加氢还原中的应用 |
TW202345696A (zh) | 2022-05-18 | 2023-12-01 | 美商科迪華農業科技有限責任公司 | 具有殺有害生物效用之組成物及與其相關的方法 |
CN115029369B (zh) * | 2022-06-01 | 2023-04-21 | 中国林业科学研究院森林生态环境与自然保护研究所 | 一种防治松材线虫病的苏云金芽孢杆菌工程菌制备方法与应用 |
WO2024044596A1 (en) | 2022-08-23 | 2024-02-29 | AgBiome, Inc. | Pesticidal genes and methods of use |
US20240093220A1 (en) | 2022-09-09 | 2024-03-21 | Friedrich Alexander Universität Erlangen-Nürnberg | Plant regulatory elements and uses thereof |
Family Cites Families (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3346138A1 (de) * | 1983-12-21 | 1985-07-11 | Boehringer Mannheim Gmbh, 6800 Mannheim | Bacillus thuringiensis var. tenebrionis sowie ein insektizid wirkendes, hieraus erhaeltliches praeparat bzw. toxin sowie deren verwendung zur bekaempfung von coleoptera |
US4771131A (en) * | 1985-08-16 | 1988-09-13 | Mycogen Corporation | Cloning and expression of Bacillus thuringiensis toxin gene encoding a protein toxic to beetles of the order Coleoptera |
GB8526774D0 (en) * | 1985-10-30 | 1985-12-04 | Sandoz Ltd | Bacillus thuringiensis hybrids |
US5338544A (en) * | 1987-04-16 | 1994-08-16 | Ecogen Inc. | CryIIB protein, insecticidal compositions and methods of use thereof |
US5024837A (en) * | 1987-05-06 | 1991-06-18 | Donovan William P | Coleopteran active microorganisms, related insecticide compositions and methods for their production and use |
US4910016A (en) * | 1987-08-03 | 1990-03-20 | Mycogen Corporation | Novel Bacillus thuringiensis isolate |
NZ226442A (en) * | 1987-10-13 | 1991-08-27 | Lubrizol Genetics Inc | Anti-coleopteran toxin and gene |
GB8800652D0 (en) * | 1988-01-13 | 1988-02-10 | Ici Plc | Bacterial strain |
US4999192A (en) * | 1988-02-12 | 1991-03-12 | Mycogen Corporation | Novel coleopteran-active bacillus thuringiensis isolate |
IL89307A0 (en) * | 1988-02-19 | 1989-09-10 | Ecogen Inc | Bacillus thuringiensis var.israelensis cryd toxin gene,protein and related insecticide compositions |
US4966765A (en) * | 1988-02-23 | 1990-10-30 | Mycogen Corporation | Novel coleopteran-active Bacillus thuringiensis isolate |
US4996155A (en) * | 1988-03-04 | 1991-02-26 | Mycogen Corporation | Bacillus thuringiensis gene encoding a coleopteran-active toxin |
US5071654A (en) * | 1988-09-01 | 1991-12-10 | Ecogen Inc. | Ion channel properties of delta endotoxins |
EP0382990A1 (en) * | 1989-02-15 | 1990-08-22 | Plant Genetic Systems, N.V. | Strains of bacillus thuringiensis |
GB8910624D0 (en) * | 1989-05-09 | 1989-06-21 | Ici Plc | Bacterial strains |
SK281286B6 (sk) * | 1989-11-17 | 2001-02-12 | Novo Nordisk A/S | Mutant mikroorganizmu bacillus thuringiensis deponovaný ako subsp. tenebrionis dsm 5480, spôsob jeho prípravy a pesticídny prostriedok, ktorý ho obsahuje |
US5173409A (en) * | 1989-12-08 | 1992-12-22 | Ecogen Inc. | Recovery of bt endotoxin protein from lysed cell mixtures |
US5187091A (en) * | 1990-03-20 | 1993-02-16 | Ecogen Inc. | Bacillus thuringiensis cryiiic gene encoding toxic to coleopteran insects |
US5143905A (en) * | 1990-05-03 | 1992-09-01 | The Regents Of The University Of California | Method and means for extending the host range of insecticidal proteins |
US5384253A (en) * | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
US5436002A (en) * | 1991-01-29 | 1995-07-25 | Mycogen Corporation | Bacillus thuringiensisisolate PS201T6 toxin |
AU649785B2 (en) * | 1991-01-31 | 1994-06-02 | Monsanto Technology Llc | Bacillus thuringiensis cryIIIC(b) toxin gene and protein toxic to coleopteran insects |
US5264364A (en) * | 1991-01-31 | 1993-11-23 | Ecogen Inc. | Bacillus thuringiensis cryIIIc(B) toxin gene and protein toxic to coleopteran insects |
AT397346B (de) | 1991-02-01 | 1994-03-25 | Gabriel Franz | Anordnung zur abschirmung von bzw. zum schutz vor erdstrahlen |
US5262324A (en) * | 1991-11-06 | 1993-11-16 | Mycogen Corporation | Coleopteran-active Bacillus thuringiensis isolates and genes encoding coleopteran-active toxins |
US5308760A (en) * | 1992-01-10 | 1994-05-03 | Washington Research Foundation | Crystal proteins of Bacillus thuringiensis, genes encoding them, and host expressing them |
JP3230840B2 (ja) * | 1992-06-10 | 2001-11-19 | 株式会社日立製作所 | 腐食環境センサー及び腐食環境制御装置 |
US5879676A (en) * | 1992-12-15 | 1999-03-09 | Abbott Laboratories | Bacillus thuringiensis strains active against lepidopteran and coleopteran pests |
EP1227154A3 (en) * | 1992-12-15 | 2003-05-14 | Abbott Laboratories | Bacillus thuringiensis strains active against lepidopteran and coleopteran pests |
WO1994016079A2 (en) * | 1992-12-31 | 1994-07-21 | Mycogen Corporation | Novel bacillus thuringiensis toxins active against corn rootworm larvae |
US5356623A (en) * | 1993-03-17 | 1994-10-18 | Ecogen Inc. | Bacillus thuringiensis cryET1 toxin gene and protein toxic to lepidopteran insects |
US5441884A (en) * | 1993-07-08 | 1995-08-15 | Ecogen Inc. | Bacillus thuringiensis transposon TN5401 |
GB9318207D0 (en) * | 1993-09-02 | 1993-10-20 | Sandoz Ltd | Improvements in or relating to organic compounds |
US5593881A (en) * | 1994-05-06 | 1997-01-14 | Mycogen Corporation | Bacillus thuringiensis delta-endotoxin |
US5527883A (en) * | 1994-05-06 | 1996-06-18 | Mycogen Corporation | Delta-endotoxin expression in pseudomonas fluorescens |
GB9605222D0 (en) * | 1996-03-12 | 1996-05-15 | Secr Defence | Clostridium perfringens epsilon toxin vaccines |
US6063756A (en) * | 1996-09-24 | 2000-05-16 | Monsanto Company | Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor |
US6093695A (en) * | 1996-09-26 | 2000-07-25 | Monsanto Company | Bacillus thuringiensis CryET29 compositions toxic to coleopteran insects and ctenocephalides SPP |
US6551962B1 (en) * | 2000-10-06 | 2003-04-22 | Monsanto Technology Llc | Method for deploying a transgenic refuge |
-
1996
- 1996-09-24 US US08/718,905 patent/US6063756A/en not_active Expired - Lifetime
-
1997
- 1997-09-24 DE DE69735776T patent/DE69735776T2/de not_active Expired - Lifetime
- 1997-09-24 BR BRPI9713219-5A patent/BR9713219B1/pt not_active IP Right Cessation
- 1997-09-24 UA UA99042264A patent/UA75317C2/uk unknown
- 1997-09-24 AR ARP970104383A patent/AR010993A1/es active IP Right Grant
- 1997-09-24 MX MXPA99002819A patent/MXPA99002819A/es active IP Right Grant
- 1997-09-24 TR TR1999/00650T patent/TR199900650T2/xx unknown
- 1997-09-24 EP EP97910734A patent/EP1015592B1/en not_active Expired - Lifetime
- 1997-09-24 KR KR1019990702527A patent/KR20000048593A/ko not_active Application Discontinuation
- 1997-09-24 UY UY24725A patent/UY24725A1/es not_active Application Discontinuation
- 1997-09-24 AU AU48033/97A patent/AU741704B2/en not_active Ceased
- 1997-09-24 CN CNB971800022A patent/CN100473725C/zh not_active Expired - Lifetime
- 1997-09-24 WO PCT/US1997/017600 patent/WO1998013498A1/en active IP Right Grant
- 1997-09-24 CA CA002267665A patent/CA2267665C/en not_active Expired - Lifetime
- 1997-09-24 JP JP51600998A patent/JP2001523944A/ja active Pending
- 1997-09-24 IL IL12916097A patent/IL129160A0/xx unknown
- 1997-09-24 US US09/147,992 patent/US6326351B1/en not_active Expired - Lifetime
- 1997-09-24 AT AT97910734T patent/ATE324447T1/de active
- 1997-09-24 PL PL97332414A patent/PL189474B1/pl unknown
- 1997-09-24 CO CO97055726A patent/CO4650229A1/es unknown
-
2000
- 2000-04-14 US US09/550,497 patent/US6248536B1/en not_active Expired - Lifetime
- 2000-04-14 US US09/549,839 patent/US6399330B1/en not_active Expired - Lifetime
-
2001
- 2001-09-10 US US09/949,972 patent/US6949626B2/en not_active Expired - Lifetime
-
2002
- 2002-06-04 US US10/162,464 patent/US7385107B2/en not_active Expired - Fee Related
-
2005
- 2005-09-20 US US11/231,104 patent/US7504229B2/en not_active Expired - Fee Related
-
2006
- 2006-06-30 AR ARP060102842A patent/AR054156A2/es not_active Application Discontinuation
-
2008
- 2008-06-09 US US12/135,475 patent/US20080274502A1/en not_active Abandoned
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107074974A (zh) * | 2014-10-16 | 2017-08-18 | 孟山都技术有限公司 | 对鳞翅目害虫具有毒性或抑制性的新型嵌合杀昆虫蛋白质 |
CN107074974B (zh) * | 2014-10-16 | 2022-01-11 | 孟山都技术有限公司 | 对鳞翅目害虫具有毒性或抑制性的嵌合杀昆虫蛋白质 |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN100473725C (zh) | 苏云金芽孢杆菌CryET33和CryET34组合物及其使用 | |
RU2382822C2 (ru) | ИНСЕКТИЦИДНЫЕ БЕЛКИ, ВЫДЕЛЕННЫЕ ИЗ ВИДОВ БАКТЕРИЙ Bacillus, И ИХ ПРИМЕНЕНИЕ | |
US7070982B2 (en) | Polynucleotide compositions encoding broad spectrum delta-endotoxins | |
US7618942B2 (en) | Method of using broad-spectrum delta-endotoxins | |
JP4389075B2 (ja) | 広域スペクトルのデルタ−エンドトキシン | |
US7655838B2 (en) | Insect inhibitory Bacillus thuringiensis proteins, fusions, and methods of use therefor | |
KR20000048680A (ko) | 갑충류 곤충과 크테노세팔라이드 종에 독성인 바실러스 투린지엔시스 씨알와이이티29 조성물들 | |
CN1360632A (zh) | 鞘翅目毒性多肽组合物和抗虫转基因植物 | |
CN101133079A (zh) | 来自苏云金芽孢杆菌的分泌的杀虫蛋白质和基因组合物及其用途 | |
US20230183300A1 (en) | MODIFIED Cry1Ca TOXINS USEFUL FOR CONTROL OF INSECT PESTS | |
US5665595A (en) | Immunoglobulins against insect tissue | |
US11761015B2 (en) | Binary insecticidal Cry toxins | |
WO2002014517A1 (en) | BROAD-SPECTRUM δ-ENDOTOXINS |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C53 | Correction of patent of invention or patent application | ||
COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: ECOGEN, INC. TO: MONSANTO CO. |
|
CP03 | Change of name, title or address |
Address after: American Missouri Applicant after: Monsanto, Co. Address before: Pennsylvania Applicant before: Ecogen, Inc. |
|
ASS | Succession or assignment of patent right |
Owner name: MONSANTO TECHNOLOGY LLC Free format text: FORMER OWNER: PHARMACIA CORP. Effective date: 20020306 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20020306 Address after: American Missouri Applicant after: Monsanto Technology Co., Ltd. Address before: American Missouri Applicant before: Pharmacia Corp. |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CX01 | Expiry of patent term |
Granted publication date: 20090401 |
|
CX01 | Expiry of patent term |