CN100402472C - Biological organic fertilizer, preparing process and use thereof - Google Patents
Biological organic fertilizer, preparing process and use thereof Download PDFInfo
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- CN100402472C CN100402472C CNB2005100891740A CN200510089174A CN100402472C CN 100402472 C CN100402472 C CN 100402472C CN B2005100891740 A CNB2005100891740 A CN B2005100891740A CN 200510089174 A CN200510089174 A CN 200510089174A CN 100402472 C CN100402472 C CN 100402472C
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- microbial inoculum
- organic fertilizer
- fertilizer
- biological organic
- bacillus cereus
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Abstract
The invention discloses a new bio-organic fertilizer and its preparation method. The bio-organic fertilizer composed of (by weight parts) organic substances 50-70. Bacillus cereus 10-30, Saccharomyces cerevisiae 8-12, and Lactobacillus acidophilus 8-12. The method comprises preparing the above three microbial agents, crushing organic fertilizer and mixing it with the microbial agents, drying, and packaging to obtain the final product. The bio-organic fertilizer is rich in a large variety of trace elements crops needed, which can effectively improve soil structure, increase granular structure and soil organic matter content, significantly improve oil fertility, and increase crop yield by 15-20%.
Description
Technical field
The present invention relates to a kind of fertilizer, relate in particular to a kind of biological organic fertilizer, Preparation Method And The Use, belong to fertilizer field.
Background technology
Use chemical fertilizer for a long time merely, easily cause the soil physico-chemical property variation, soil can become harden, permeability variation, crumb structure reduce, pH in soil can peracid or is crossed alkali, finally can cause soil fertility seriously to descend, and is unsuitable for plant growth.Fertilizer have nutritive element comprehensively, can effectively improve advantages such as Soil structure and physico-chemical property, but exist simultaneously nutrient content low, take effect and wait defective slowly.Biological organic fertilizer has overcome the defective of above-mentioned two kinds of fertilizer, has had the advantage of the two concurrently, so biological organic fertilizer has wide application prospect on agroforestry are produced.But the prior biological fertilizer all in various degree exist defectives such as the output that improves crop is remarkable inadequately.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, a kind of fertilizer efficiency height is provided, can significantly improves the biological organic fertilizer of crop yield.
Technical problem to be solved by this invention realizes by following technological approaches;
A kind of biological organic fertilizer, form by the raw material of following weight part:
Organic substance 50-70 part, bacillus cereus microbial inoculum 10-30 part, cereuisiae fermentum bacteria agent 8-12 part and Lactobacterium acidophilum microbial inoculum 8-12 part.
Be preferably: 60 parts of organic substances, 20 parts of bacillus cereus microbial inoculums, 10 parts of cereuisiae fermentum bacteria agents, 10 parts of Lactobacterium acidophilum microbial inoculums.
Described microbial inoculum is preferably the powdery microbial inoculum.
Described bacillus cereus (Bacillus cereus Frankland and Frankland) microbial inoculum, saccharomyces cerevisiae (Saccharomyces sp.) microbial inoculum and Lactobacterium acidophilum microbial inoculum [Lactobacillusacidophilus (Moro) Hansen﹠amp; Mocquot] can prepare by conventional bacterial preparation process;
Effective strain number contained in the every gram fertilizer of biological organic fertilizer of the present invention is more than or equal to 0.20 hundred million, and the assorted bacterium rate of fertilizer of the present invention is less than or equal to 10%.
Described organic substance is selected from any of following any one material or its mixture: cottonseed meal, soybean meal, peanut meal, rapeseed meal, pig manure or chicken manure are preferably cottonseed meal or soybean meal.
The used microbial strains of the present invention (can available from China Committee for Culture Collection of Microorganisms common micro-organisms center) and other raw material all can be bought from market and obtain, and its specification meets national sector standard.
Biological organic fertilizer of the present invention can prepare according to the ordinary method of biological organic fertilizer, and these all are that those skilled in the art is easy to control and realize.
Preferably, a kind of method for preparing biological organic fertilizer of the present invention, step is as follows:
1) prepares bacillus cereus microbial inoculum, cereuisiae fermentum bacteria agent and Lactobacterium acidophilum microbial inoculum by art methods;
2) take by weighing each raw material by following weight part: 50～70 parts of organic substances, bacillus cereus microbial inoculum 10-30 part, cereuisiae fermentum bacteria agent 8-12 part and Lactobacterium acidophilum microbial inoculum 8-12 part;
3) organic substance is pulverized the back mixes with above-mentioned microbial inoculum, dry, packing promptly.
Biological organic fertilizer of the present invention contains the beneficial microorganism flora, and the environmental compatibility of this flora is strong, has easily given play to the population advantage, and trophic function is strong, and rhizosphere is short living effective, the fertilizer efficiency height; Be rich in organic and inorganic nutrient, volume is little, be convenient to use, and can satisfy large-scale production and service requirements; Biological organic fertilizer of the present invention is rich in physiologically active substance; Safe and harmless.
The fertilizer efficiency of biological organic fertilizer of the present invention is the effect by the beneficial microorganism bacterium, the fertilizer efficiency of efficient organic fertilizer, the collaborative generations such as interaction of microorganism and fertilizer, have the effect of microbial inoculant and fertilizer concurrently, have the two-fold advantage of microorganism fertilizer and fertilizer and overcome their shortcomings separately, being rich in various crop grows necessary a large amount of and micro-, can effectively improve Soil structure, increase soil aggregate and organic content, increase soil fertility obviously, can increase about the output 15-20% of crop, having aborning significantly improves the soil fosters and apply fertilizer and increases production, increase the matter effect.
The using method of biological organic fertilizer of the present invention: can make base manure and topdress to use.Food crop make base manure, the application in two dressings of topdressing, and spread fertilizer over the fields or row replacement.Base manure accounts for full rate of fertilizer application 60-70%, and spread fertilizer over the fields and plough the even sowing of back rake, 3-5 centimetre of row replacement fertilising line-spacing sowing row, topdressing accounts for full rate of fertilizer application 30-40%.Use mu consumption 60-80 kilogram after turning green; Vegetable crop makes base manure with 30-40 kilogram/mu, evenly is spread on the surface, ploughs plough then and turns over to press and sow, and respectively imposes 10-15 kilogram/mu in setting phase and the best fruiting period.
Embodiment
Further describe the present invention by the following examples, it should be understood that these embodiment only are used for the purpose of illustration, never limit the scope of the invention.
[embodiment 1]
Take by weighing each raw material (unit: kg): cottonseed meal 60, bacillus cereus microbial inoculum 20, cereuisiae fermentum bacteria agent 10 and Lactobacterium acidophilum microbial inoculum 10 by following weight;
With cottonseed meal pulverize that the back mixes with above-mentioned three kinds of microbial inoculums, dry, packing promptly.
Bacillus cereus microbial inoculum, cereuisiae fermentum bacteria agent and Lactobacterium acidophilum microbial inoculum prepare the microbial inoculum method according to routine and are prepared from; Bacillus cereus bacterial classification, (beer) yeast bacterial classification and Lactobacterium acidophilum bacterial classification are all available from DSMZ of Institute of Microorganism, Academia Sinica, and its numbering is respectively: CGMCC1.932, CGMCC2.451 and CGMCC1.1854.
The preparation of three kinds of microbial inoculums can be with reference to following method:
One, preparation bacillus cereus microbial inoculum
1, prepares inclined-plane seed bacterial strain: twice of activation (be about to pure strain and be seeded in the dull and stereotyped cultivation of going up).
2, preparation seed bacterial strain in the eggplant bottle: with the bacterial strain of above-mentioned cultivation PDA substratum (potato 200g, glucose 20g, agar-agar (fat) powder 15g, water 1000ml) in 250 milliliters of eggplant bottles, cultivates, culture temperature is 24 ℃, wash with aqua sterilisa during 96 hours well-growns, as the inoculum of seeding tank.
3, press 2/3 of seeding tank volume and throw substratum, substratum consists of Semen Maydis powder 3%, soybean cake powder 3%, bran powder 3%, K
2HPO
42.15%, KH
2PO
40.8%, MgSO
40.075%, peanut oil 0.3%.Initial pH7.5.To producing a jar inoculation, begin fermentation from seeding tank.
Fermentation condition: culture temperature is 28 ℃, and ventilation is 1: 0.5, and tank pressure is 0.05mpa/cm
2, stirring velocity is 220rpm, inoculates after 20 hours and takes a sample once every 4 hours, observe the thalli growth situation, whether pollution arranged, wait to find spore generate reach more than 90 after, liquid fermenting is finished, in zymocyte liquid, add sorbent material and weighting agent, get the bacillus cereus microbial inoculum.
Two, the preparation method of cereuisiae fermentum bacteria agent and Lactobacillus acidophilus's microbial inoculum.
The preparation method of cereuisiae fermentum bacteria agent is the same; In the preparation process of Lactobacterium acidophilum microbial inoculum, the preparation method of substratum is the same, and different is in the culturing process of fermentor tank, adopts anaerobism to cultivate, and turns off air compressor, and other preparation process is the same.
[embodiment 2]
Take by weighing each raw material (unit: kg): soybean meal 50, bacillus cereus microbial inoculum 30, saccharomyces cerevisiae powdery microbial inoculum 12 and Lactobacterium acidophilum powdery microbial inoculum 8 by following weight.(preparation of bacillus cereus microbial inoculum, cereuisiae fermentum bacteria agent and lactobacillus microbial inoculum is with embodiment 1)
With soybean meal pulverize that the back mixes with above-mentioned microbial inoculum, dry, packing promptly.
[embodiment 3]
Take by weighing each raw material (unit: kg): peanut meal 70, bacillus cereus microbial inoculum 14, cereuisiae fermentum bacteria agent 8 and Lactobacterium acidophilum microbial inoculum 8 by following weight.(preparation of bacillus cereus microbial inoculum, cereuisiae fermentum bacteria agent and Lactobacterium acidophilum microbial inoculum is with embodiment 1)
With peanut meal pulverize that the back mixes with above-mentioned three kinds of microbial inoculums, dry, packing promptly.
[embodiment 4]
Take by weighing each raw material (unit: kg): rapeseed meal 30, peanut meal 30, bacillus cereus microbial inoculum 16, cereuisiae fermentum bacteria agent 12 and Lactobacterium acidophilum microbial inoculum 12 by following weight; (preparation of bacillus cereus microbial inoculum, cereuisiae fermentum bacteria agent and Lactobacterium acidophilum microbial inoculum is with embodiment 1).With rapeseed meal and peanut meal pulverize that the back mixes with above-mentioned microbial inoculum, dry, packing promptly.
The effect test of [test example 1] biological organic fertilizer of the present invention on Beijing early soybean is produced
One, test materials
1, for examination fertilizer: the biological organic fertilizer that the embodiment of the invention 1 is prepared.
2, trial crops: soybean.
Two, test site and time
Test site: experiment base, clay fertilizer institute of Chinese Academy of Agricultural Sciences Changping
Test period: year September in April, 2004-2004.
Three, test method
Experimental field be moisture soil, test preceding 4 years even ground (only plant crop, do not use any fertilizer) through this, soil fertility is lower.2 processing are established in test, are respectively and execute biological organic fertilizer of the present invention and do not apply fertilizer (contrast), three repetitions, totally 6 sub-districts, randomized block design.Sub-district area 2 * 2=4 square metre.
Biological organic fertilizer of the present invention is by 25 kilograms of mu amounts of application, 0.165 kilogram of promptly every sub-district.Fertilizer is handled and is disposable bottom application.Promptly earlier each is handled fertilizer and evenly be spread on each sub-district, turn over afterwards and bury.Sowing on April 20, every sub-district plantation 6 row soybean, final singling in tri-leaf period, 20 centimetres of spacing in the rows.September 19 gathered in the crops.
Carry out field investigation and indoor species test during results, respectively handle effective strain number, effective pod number, single-strain grain number and thousand seed weight, and meter produces.
Four, result and analysis
Table 1 is the soybean yields constituent element and the yield result of biological organic fertilizer of the present invention.By table 1 as seen: compare with the contrast of not applying fertilizer, the effective pod number of the soybean per plant of biological organic fertilizer treatment group of the present invention, single-strain grain number, thousand seed weight, that the equal yield line constituent element is counted in the effective strain in sub-district is all higher.Wherein, the effective pod number of individual plant improves 72%, and single-strain grain number improves 88.85%, and thousand seed weight improves 8.99%.Compare with the contrast of not applying fertilizer, use and show significant effect of increasing production (table 2, table 3), its amplification is 107.62%.
Table 1 biological organic fertilizer of the present invention is to the influence of soybean yields constituent element
Table 2 biological organic fertilizer of the present invention is to the influence of soybean yields
Table 3 output analysis of variance table
Test-results shows that biological organic fertilizer of the present invention can significantly improve the output of crop, and effect of increasing production is obvious.
The effect test of [test example 2] biological organic fertilizer of the present invention on the Shandong early soybean is produced
One, test materials
1, for examination fertilizer: the biological organic fertilizer that the embodiment of the invention 1 is prepared.
2, trial crops: soybean.
Two, test site and time
Test site: experiment base, Laiyang, Chinese Academy of Agricultural Sciences Shandong.
Test period: year September in April, 2004-2004.
Three, test method
Experimental field be fine sandy loam, the soil fertility below average.2 processing are established in test, are respectively and execute biological organic fertilizer of the present invention and do not apply fertilizer (contrast), three repetitions, totally 6 sub-districts, randomized block design.Sub-district area 20 * 10=200 square metre.Biological organic fertilizer of the present invention is by 25 kilograms of mu amounts of application.Fertilizer is handled and is disposable bottom application.Promptly earlier each is handled fertilizer and evenly be spread on each sub-district, turn over afterwards and bury.Sowing on April 25, every sub-district plantation 6 row soybean, final singling in tri-leaf period, 20 centimetres of spacing in the rows.September 29 gathered in the crops.Carry out field investigation and indoor species test during results, respectively handle effective strain number, effective pod number, single-strain grain number and thousand seed weight, and meter produces.
Four, test results and analysis
Table 4 is soybean yields constituent element and yield result of biological organic fertilizer of the present invention.By table 4 as seen: compare with the contrast of not applying fertilizer, the effective pod number of the soybean per plant of biological organic fertilizer treatment group of the present invention, single-strain grain number, thousand seed weight equal yield line constituent element are all higher.Wherein, the effective pod number of individual plant improves 69.3%, and single-strain grain number improves 26.5%, and thousand seed weight improves 28.9%.Compare with the contrast of not applying fertilizer, use and show significant effect of increasing production (table 5, table 6), its amplification is 64.8%.
Table 4 biological organic fertilizer of the present invention is to the influence of soybean yields constituent element
Table 5 biological organic fertilizer of the present invention is to the influence of soybean yields
Table 6 output analysis of variance table
Test-results shows that biological organic fertilizer of the present invention can significantly improve the output of crop, and effect of increasing production is obvious.
Claims (5)
1. biological organic fertilizer, form by the raw material of following weight part:
Organic substance 50-70 part, bacillus cereus (Bacillus cereus Frankland andFrankland) microbial inoculum 10-30 part, saccharomyces cerevisiae (Saccharomyces sp.) microbial inoculum 8-12 part and Lactobacterium acidophilum [Lactobacillus acidophilus (Moro) Hansen﹠amp; Mocquot] microbial inoculum 8-12 part; Described organic substance is selected from any of following any one material or its mixture: cottonseed meal, soybean meal, peanut meal, rapeseed meal, chicken manure or pig manure.
2. according to the biological organic fertilizer of claim 1, it is characterized in that the weight part of each raw material is: 60 parts of organic substances, 20 parts of bacillus cereus microbial inoculums, 10 parts of cereuisiae fermentum bacteria agents, 10 parts of Lactobacterium acidophilum microbial inoculums.
3. according to the biological organic fertilizer of claim 1, it is characterized in that described microbial inoculum is the powdery microbial inoculum.
4. method for preparing the biological organic fertilizer of claim 1, step is as follows:
1) prepares bacillus cereus microbial inoculum, cereuisiae fermentum bacteria agent and Lactobacterium acidophilum microbial inoculum by art methods;
2) take by weighing each raw material by following weight part: 50～70 parts of organic substances, bacillus cereus microbial inoculum 10-30 part, cereuisiae fermentum bacteria agent 8-12 part and Lactobacterium acidophilum microbial inoculum 8-12 part;
3) organic substance is pulverized the back mixes with above-mentioned microbial inoculum, dry, packing promptly.
5. the purposes of the described biological organic fertilizer of claim 1 in agroforestry are produced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100891740A CN100402472C (en) | 2005-08-05 | 2005-08-05 | Biological organic fertilizer, preparing process and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100891740A CN100402472C (en) | 2005-08-05 | 2005-08-05 | Biological organic fertilizer, preparing process and use thereof |
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| Publication Number | Publication Date |
|---|---|
| CN1907917A CN1907917A (en) | 2007-02-07 |
| CN100402472C true CN100402472C (en) | 2008-07-16 |
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| CNB2005100891740A Expired - Fee Related CN100402472C (en) | 2005-08-05 | 2005-08-05 | Biological organic fertilizer, preparing process and use thereof |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102115347A (en) * | 2010-12-12 | 2011-07-06 | 夏学德 | Mixture fertilizer and preparation method thereof |
| CN103274838B (en) * | 2013-06-09 | 2014-06-18 | 苏州仁成生物科技有限公司 | Multi-element compound fertilizer of leontopodium smithianum and preparation method thereof |
| CN105940807B (en) * | 2016-05-12 | 2017-12-22 | 巢湖市鑫宇良种农民专业合作社 | A kind of method using Agricultural residues improved soil |
| CN106748407A (en) * | 2016-11-16 | 2017-05-31 | 江苏益客食品有限公司 | Biological organic fertilizer and its preparation method and application |
| CN106699252A (en) * | 2016-11-16 | 2017-05-24 | 江苏益客食品有限公司 | Biological organic fertilizer and preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61270290A (en) * | 1985-05-22 | 1986-11-29 | 株式会社 ア−ゼロンジヤパン | Chitin-containing substance-fermented organic fertilizer |
| JP2001199782A (en) * | 2000-01-12 | 2001-07-24 | Marutomo:Kk | Method for producing fertilizer and compost having crumb structure |
| CN1407086A (en) * | 2001-08-30 | 2003-04-02 | 武军勇 | Composite cellulose decomposition bacteria and preparation and use thereof |
| CN1605579A (en) * | 2004-11-11 | 2005-04-13 | 天津大学 | Rapid microbial zymophyte agent and zymolysis process for chicken manure |
-
2005
- 2005-08-05 CN CNB2005100891740A patent/CN100402472C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61270290A (en) * | 1985-05-22 | 1986-11-29 | 株式会社 ア−ゼロンジヤパン | Chitin-containing substance-fermented organic fertilizer |
| JP2001199782A (en) * | 2000-01-12 | 2001-07-24 | Marutomo:Kk | Method for producing fertilizer and compost having crumb structure |
| CN1407086A (en) * | 2001-08-30 | 2003-04-02 | 武军勇 | Composite cellulose decomposition bacteria and preparation and use thereof |
| CN1605579A (en) * | 2004-11-11 | 2005-04-13 | 天津大学 | Rapid microbial zymophyte agent and zymolysis process for chicken manure |
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