CN104130050B - Multielement composite microbe fertilizer and its production method - Google Patents
Multielement composite microbe fertilizer and its production method Download PDFInfo
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- CN104130050B CN104130050B CN201410324985.3A CN201410324985A CN104130050B CN 104130050 B CN104130050 B CN 104130050B CN 201410324985 A CN201410324985 A CN 201410324985A CN 104130050 B CN104130050 B CN 104130050B
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- Prior art keywords
- potassium
- powder
- humic acid
- standby
- nitrogen
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 42
- 239000002131 composite material Substances 0.000 title claims abstract description 27
- 238000004519 manufacturing process Methods 0.000 title claims description 8
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical group [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 66
- 239000011591 potassium Substances 0.000 claims abstract description 66
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 66
- 239000000843 powder Substances 0.000 claims abstract description 43
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 42
- 241000894006 Bacteria Species 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 29
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000004021 humic acid Substances 0.000 claims abstract description 26
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000011574 phosphorus Substances 0.000 claims abstract description 23
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 23
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 21
- 235000004443 Ricinus communis Nutrition 0.000 claims abstract description 20
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 9
- 239000000084 colloidal system Substances 0.000 claims abstract description 9
- 241000187809 Frankia Species 0.000 claims abstract description 8
- 238000007605 air drying Methods 0.000 claims abstract description 8
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 7
- 238000010564 aerobic fermentation Methods 0.000 claims abstract description 7
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229910001950 potassium oxide Inorganic materials 0.000 claims abstract description 7
- 230000004913 activation Effects 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 238000000855 fermentation Methods 0.000 claims description 21
- 230000004151 fermentation Effects 0.000 claims description 21
- 239000000470 constituent Substances 0.000 claims description 17
- 239000002609 medium Substances 0.000 claims description 16
- 229910052564 epsomite Inorganic materials 0.000 claims description 15
- 229930006000 Sucrose Natural products 0.000 claims description 14
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 14
- 239000012153 distilled water Substances 0.000 claims description 14
- 239000005720 sucrose Substances 0.000 claims description 14
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 12
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 12
- 229910000162 sodium phosphate Inorganic materials 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 11
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 10
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 10
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- 241000726221 Gemma Species 0.000 claims description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 238000000386 microscopy Methods 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 230000003381 solubilizing effect Effects 0.000 claims description 9
- 241001052560 Thallis Species 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 8
- 229940088594 vitamin Drugs 0.000 claims description 8
- 235000013343 vitamin Nutrition 0.000 claims description 8
- 239000011782 vitamin Substances 0.000 claims description 8
- 229930003231 vitamin Natural products 0.000 claims description 8
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 8
- 239000011573 trace mineral Substances 0.000 claims description 7
- 235000013619 trace mineral Nutrition 0.000 claims description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 6
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 6
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 6
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 6
- 238000010899 nucleation Methods 0.000 claims description 6
- 239000005416 organic matter Substances 0.000 claims description 6
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 claims description 6
- 239000008107 starch Substances 0.000 claims description 6
- 235000019698 starch Nutrition 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 239000007640 basal medium Substances 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- 238000005516 engineering process Methods 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- 239000010452 phosphate Substances 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 3
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 3
- 239000007836 KH2PO4 Substances 0.000 claims description 3
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 3
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 3
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims description 3
- 238000003556 assay Methods 0.000 claims description 3
- 229960002685 biotin Drugs 0.000 claims description 3
- 235000020958 biotin Nutrition 0.000 claims description 3
- 239000011616 biotin Substances 0.000 claims description 3
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 229960002079 calcium pantothenate Drugs 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims description 3
- 239000013530 defoamer Substances 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 3
- 239000006052 feed supplement Substances 0.000 claims description 3
- 239000006260 foam Substances 0.000 claims description 3
- 229960000304 folic acid Drugs 0.000 claims description 3
- 235000019152 folic acid Nutrition 0.000 claims description 3
- 239000011724 folic acid Substances 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 239000011565 manganese chloride Substances 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 229960003512 nicotinic acid Drugs 0.000 claims description 3
- 235000001968 nicotinic acid Nutrition 0.000 claims description 3
- 239000011664 nicotinic acid Substances 0.000 claims description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 3
- 229920000053 polysorbate 80 Polymers 0.000 claims description 3
- 229960002477 riboflavin Drugs 0.000 claims description 3
- 239000002151 riboflavin Substances 0.000 claims description 3
- 235000019192 riboflavin Nutrition 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 239000011684 sodium molybdate Substances 0.000 claims description 3
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 3
- 229940054269 sodium pyruvate Drugs 0.000 claims description 3
- 230000028070 sporulation Effects 0.000 claims description 3
- 229910000391 tricalcium phosphate Inorganic materials 0.000 claims description 3
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 3
- 239000008158 vegetable oil Substances 0.000 claims description 3
- 230000000007 visual effect Effects 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 239000011686 zinc sulphate Substances 0.000 claims description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 2
- 229920001817 Agar Polymers 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 238000012360 testing method Methods 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 241000170793 Phalaris canariensis Species 0.000 claims 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 1
- 238000002386 leaching Methods 0.000 claims 1
- 229910052603 melanterite Inorganic materials 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 4
- 239000011435 rock Substances 0.000 abstract description 3
- 241001494106 Stenotomus chrysops Species 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 9
- 239000002585 base Substances 0.000 description 6
- 244000005700 microbiome Species 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 241000881860 Paenibacillus mucilaginosus Species 0.000 description 3
- 239000007633 bacillus mucilaginosus Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 3
- 235000010755 mineral Nutrition 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- DLHONNLASJQAHX-UHFFFAOYSA-N aluminum;potassium;oxygen(2-);silicon(4+) Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[Al+3].[Si+4].[Si+4].[Si+4].[K+] DLHONNLASJQAHX-UHFFFAOYSA-N 0.000 description 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 235000019797 dipotassium phosphate Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 235000002867 manganese chloride Nutrition 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 235000015393 sodium molybdate Nutrition 0.000 description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 2
- 235000009529 zinc sulphate Nutrition 0.000 description 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000736262 Microbiota Species 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- RDXARWSSOJYNLI-UHFFFAOYSA-N [P].[K] Chemical compound [P].[K] RDXARWSSOJYNLI-UHFFFAOYSA-N 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 238000004178 biological nitrogen fixation Methods 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 238000005065 mining Methods 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
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- 238000012827 research and development Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 238000009923 sugaring Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
It is potassium decomposing strain using the potassium shale rich in potassium oxide and humic acid as primary raw material, with colloid bacillus cereus SCUP 76 8 that a kind of multielement composite microbe fertilizer, which is, using bacillus megaterium as phosphorus decomposing strain, using frankia fgc6 as fixed nitrogen strain, potassium shale and humic acid are carried out that the multielement composite microbe fertilizer based on potassium is made after fermented and cultured respectively.The plain composite microbe fertilizer of the present invention first prepares potassium shale powder, humic acid powder, castor cake powder, potassium decomposing fermented liquid, phosphorus decomposing fermented liquid, fixed nitrogen fermented liquid respectively, mixed by the mass volume ratio of potassium page rock powder ︰ potassium decomposing fermented liquid ︰ phosphorus decomposing fermented liquids for the ︰ 0.8 of 1 ︰ 1, under certain condition aerobic fermentation natural air drying after 79 days；Mixed by the mass volume ratio of humic acid powder ︰ castor cake powder ︰ fixed nitrogen fermented liquids for the ︰ 1 of 58 ︰ 1, under certain condition aerobic fermentation natural air drying after 10 12 days；It is finished product activation potassium shale powder and nitrogen-fixing bacteria humic acid and castor cake powder 58 63 ︰ 37 42 in mass ratio to be mixed after drying.
Description
Technical field
The present invention relates to a kind of composite fertilizer, more particularly to one kind using potassium feldspar as primary raw material, rich in efficient to potassium feldspar
The multiple element composite microbe fertilizer and its production method of potassium decomposing, Soluble phosphorus and nitrogen-fixing microorganism.
Background technology
Growth and development of plants needs the different element of a variety of quantity, it is especially desirable to substantial amounts of nitrogen, phosphorus, potassium element.To meet
People's demand growing to various plants, traditional organic fertilizer obviously can not meet the needs of plant, therefore, industrial metaplasia
It is considered as to realize this purpose main path always to produce substantial amounts of nitrogen, phosphorus, potassium chemical fertilizer.However, with fertilizer application amount
Continuous increase, its negative effect is increasingly apparent, outstanding behaviours be the hardening soil, soil degradation, soil and underground water it is seriously dirty
Dye, soil texture and microbiota and diversity by destroy, crops quality declines, it is serious when jeopardize food security
Deng.So many limitations have been made in use of the World Food Programme to chemical fertilizer, some developed countries and regions even start to prohibit
Only use chemical fertilizer.
As the development of science and technology and the control increasingly increased of Environmental are paid attention to, China is in novel plant fertilizer
Also substantial amounts of work has been made in research and development.It has been reported that and the various new-type fertilizers that use it is a lot, have and planted with corruption
Organic fertilizer based on acid, have one or more microorganism groups into composite microbe fertilizer, have biology with mineral compound fertilizer etc..This hair
It is bright to belong to one of biology and mineral compound fertilizer.CN100343202C discloses a kind of biological multi element phosphorus potassium ferbilizer, and potassium shale is crushed
Dissolving phosphor and dissolving potassium bacterium after fermentation is added afterwards to be made, is less containing element the defects of the fertilizer, and effective potassium content is also insufficient.Application
People discloses a kind of biological mineral compound fertilizer and its production method (CN101475417A), by 70%-80% potassium shale powder with
20%-30% humic acid is made after the fermentation of potassium decomposing, phosphorus decomposing and ferment bacterium, and due to existing, the ability of potassium solubilizing bacteria is weaker and potassium shale
The problems such as accounting in composite fertilizer is larger, cause effective potassium for being discharged from potassium shale relatively low, the improvement soil of composite fertilizer
Combination property is smaller, and application is subject to certain restrictions.Further to improve the utilization rate of potassium shale, fertilizer efficiency is improved, is improved
The combination property of composite fertilizer, expand application, applicant screens wild type indigenous strain from mining area, and has carried out mutation breeding
Studied with fermentation technique, it was found that the powerful bacterial strain of ability of dissolving potassium, and this application and development is gone out into multielement composite microbe fertilizer.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of multielement composite microbe fertilizer and its production method, and this is more
Elements compounding microorganism fertilizer improves the utilization rate of potassium shale, improves the combination property of composite fertilizer, improves fertilizer efficiency, expands
Application.
In order to solve the above technical problems, the potassium shale and humic acid rich in potassium oxide that the applicant location abounds with are original
Material, the colloid bacillus cereus using the powerful deposit number of ability of dissolving potassium as CGMCCNo.8481（Bacillus mucilaginosus）HSCUP-76-8 as potassium decomposing strain, using the strong deposit number of dissolving P capacity as CGMCC No.4296's
Bacillus megaterium (Bacillus megaterium) as phosphorus decomposing strain, using deposit number as CCTCC M207197 not
Blue kirschner bacterium (Frankia.sp) fgc6 bacterial strains are as fixed nitrogen strain, after carrying out fermented and cultured to potassium shale and humic acid respectively,
It is made rich in the multielement composite microbe fertilizer based on potassium.
Multielement composite microbe fertilizer of the present invention is to be prepared according to the following steps：
(1) prepare potassium shale powder and choose potassium shale of the potassium oxide content more than 8%, be ground into 150-250 mesh, it is standby；
(2) prepare humic acid powder and take humic acid of the content of organic matter more than 55%, be ground into 100-150 mesh, it is standby；
(3) preparing castor cake powder takes castor cake to be ground into 80-100 mesh, standby；
(4) preparation potassium decomposing fermented liquid, which goes bail for hide, numbers the colloid bacillus cereus for being CGMCCNo.8481（Bacillus mucilaginosus）HSCUP-76-8 carries out high density fermentation preparation as potassium solubilizing bacteria, and concrete technology step is：
Colloid bacillus cereus HSCUP-76-8 is inoculated in slant medium and activated by A, temperature control at 29-33 DEG C,
24-48 hours are cultivated, obtain original bevel seed, then original bevel seed is inoculated in slant medium, the same terms culture,
Inclined-plane seed must be produced；
B is inoculated in inclined-plane seed is produced in fluid nutrient medium, and 20%-45% liquid is loaded in 200-500mL container
Body culture medium, 29-33 DEG C of shaking table culture temperature, rotating speed 160-220r/min, incubation time 8-12 hours, obtain seed liquor；
Seed liquor is inoculated in seed tank culture base by C, coefficient 0.7-0.8,121 DEG C of steam sterilizings 20 minutes, inoculation
Measure 5%-10%, 29-33 DEG C of cultivation temperature, throughput 1.0：0.8-1.0（v/v·min）, dissolved oxygen saturation is controlled with speed of agitator
Spend DO values 10%-30%, pH controls are cultivated 8~12 hours, acquisition seeding tank seed liquor 7.2 ± 0.2；
Seeding tank seed liquor is inoculated in fermentation basal medium by D, coefficient 0.7-0.8,121 DEG C of steam sterilizings
20 minutes, inoculum concentration 5%-10%, control parameter：29-33 DEG C of temperature, pH value 7.0-7.2, throughput 1：0.8-1.2（v/v·
min）, dissolved oxygen saturation degree DO value 20%-30% are controlled with speed of agitator, foam is controlled by defoamer of vegetable oil；It is small at interval of 4
When take a sample, make microscopy observation bacterium number and thalli morphology, chemical examination sugar, ammoniacal nitrogen, according to the in good time feed supplement of assay, control immediately
Sugaring dosage is 2g/L-3g/L, with NH4Cl controls nitrogen source 0.5g/L-0.1g/L, cultivates 12-14 hours, stops mending nitrogen, then
Adjust control parameter：33-37 DEG C of temperature, pH value 7.2-8.9, throughput 1：1.0-0.8（v/v·min）, with speed of agitator
Dissolved oxygen saturation degree DO values 5%~10% are controlled, promotes sporulation, a sample was taken at interval of 4 hours, make microscopy observation thalline immediately
Form, when the thalli morphology more than 98% in the microscopy visual field is gemma, stop fermented and cultured, gemma number reaches 1.8 × 109
cfu/mL-2.0×109Cfu/mL, gemma conversion ratio are standby more than 75%；
The constituent of described slant medium is：Sucrose 5-6g, NaH2PO4 1-1.5g、MgSO4·7H2O 0.3-
0.5g、FeCl3 0.004-0.005g、CaCO3 0-0.1g, agar 20-30g, distilled water 1000mL, pH 7.0-7.4；
The constituent of described fluid nutrient medium is：Sucrose 5-10g, NH4Cl 0.5-1g、NaH2PO4 1-1.5g、
MgSO4·7H2O 0.5-1g、FeCl3 0.005g、CaCO3 0.1-0.3g, distilled water 1000mL, pH 7.0-7.4；
The constituent of described seed tank culture base is：Sucrose 2-5g, starch 3-10g, (NH4)2SO4 1-2g/
NH4Cl 0.5-1g, yeast extract 1-2g, NaH2PO4 1-1.5g、MgSO4·7H2O 0.5-1g、FeCl30.005g, distilled water
1000mL, pH 7.0-7.4；
The constituent of described fermentation basal medium is：Sucrose 5g, (NH4)2SO4 1-2g/ NH4Cl 0.5-
1g, yeast extract 1-2g, NaH2PO4 1-2g、MgSO4·7H2O 0.5-1g、FeCl30.005g, distilled water 1000mL, pH
7.0-7.4；Wherein described sucrose can with corn flour, starch any one or more equivalent replace；
(5) preparation phosphorus decomposing fermented liquid, which goes bail for hide, numbers the bacillus megaterium (Bacillus for being CGMCC No.4296
Megaterium high density fermentation preparation) is carried out as phosphate solubilizing bacteria, the constituent of phosphorus decomposing bacterium culture medium is：Glucose 8-
10g, (NH4)2SO40.5-0.8g, NaCl 0.3-0.5g, MgSO4·7H2O 0.3-0.5g, KCl 0.3-0.5g, FeSO4·
7H2O 0.03-0.05g, MnSO4·4H2O 0.03-0.05g, Ca3(PO4)25-6g, distilled water 1000mL, pH7.0-7.2；Hair
32-48 hours ferment time reach 4.5 × 109More than cfu/mL, stop fermented and cultured, it is standby；
(6) prepare fixed nitrogen fermented liquid go bail for hide numbering be CCTCC M207197 frankia
(Frankia.sp) for fgc6 bacterial strains as nitrogen-fixing bacteria, fermentation prepares the constituent of the culture medium of high density bacterium solution by gross mass point
Number 100% is counted, glucose 0.03%-0.08%, Tween 80 0.003%-0.008%, Sodium Pyruvate 0.03%-0.08%,
(NH4) 2SO40.05%-0.1%, KH2PO4 0.095%, K2HPO4 0.0435%, MgSO47H2O 0.0096%,
CaCl2 0.001%, FeSO4 0.00056%, NaEDTA 0.00075%, trace element water-soluble liquid 0.1%, vitamin is water-soluble
Liquid 0.1%, surplus are water；Trace element water-soluble liquid composition therein is H3BO3 0.286% based on total mass fraction 100%,
MnCl2 0.181%, ZnSO4 0.022%, CuSO4 0.008%, Na2MoO4 0.0025%, CoCl2 0.0025% and remaining
Measure as water；The vitamin aqueous solution is formed based on total mass fraction 100%, is vitamin V B1 0.001%, nicotinic acid 0.005%,
Biotin 0.0015%, folic acid 0.001%, calcium pantothenate 0.001%, riboflavin 0.001% and surplus are water；First 28-30
After being cultivated 10-15 days at DEG C, then culture 72-96 hours obtain the hair that dry cell weight is more than 300 μ g/mL at 28-30 DEG C
Zymotic fluid, it is standby；
(7) it is the ︰ 0.8 of 1 ︰ 1 by the mass volume ratio of potassium page rock powder ︰ potassium decomposing fermented liquid ︰ phosphorus decomposing fermented liquids, (1) is made
Phosphorus decomposing bacterium solution prepared by potassium decomposing bacterium solution prepared by standby potassium shale powder, (4), (5) is mixed evenly, in 34-36 DEG C, humidity
Under the conditions of 65%-75%, aerobic fermentation 7-9 days, then natural air drying, standby；
(8) it is the ︰ 1 of 5-8 ︰ 1 by the mass volume ratio of humic acid powder ︰ castor cake powder ︰ fixed nitrogen fermented liquids, is prepared by (2)
Nitrogen-fixing bacteria prepared by humic acid powder, the castor cake powder of (3) preparation, (7) are mixed evenly, in 28-30 DEG C, humidity 65%-75%
Under the conditions of, aerobic fermentation 10-12 days, then natural air drying, standby；
(9) nitrogen-fixing bacteria humic acid and castor cake powder prepared by the activation potassium shale powder prepared (7) and (8) is in mass ratio
58-63 ︰ 37-42 are well mixed, and are dried below 35 DEG C to moisture and are less than or equal to 8%, as finished product.
After testing, the multielement composite microbe fertilizer produced according to the method for the present invention contains following component：Organic matter
10%-12%, potassium oxide 5%-7%, sulphur 0.7%-0.8%, calcium 5%-8%, magnesium 0.4%-0.5%, boron 0.1%-0.3%, iron 1.5%-3%, copper
0.2%-0.3%, zinc 0.1%-0.3%, manganese 0.2%-0.4%, molybdenum 0.3%-0.4 $, potassium solubilizing bacteria 1.4-1.7%, phosphate solubilizing bacteria 1.2%-1.4%,
Nitrogen-fixing bacteria 1.5%-1.9%, water 7%-8%, surplus are silica.
Humic acid and castor cake powder of the multi-elements composite fertilizer of the present invention from the potassium shale of rich potassium and rich in organic matter
, will using biotechnology respectively in the presence of potassium decomposing fermented liquid, phosphorus decomposing fermented liquid and fixed nitrogen fermented liquid as raw material
The potassium of potassium shale and soil nonabsorable, phosphorus etc. discharge member and usually directly absorbed for crop.The multielement of the present invention is answered
The a large amount of various microorganisms contained in Hefei promote field biological circle soil, plant, animal and the mass exchange of microorganism with it is good
Property circulation, the effect of reaching improved soil, optimize the environment.
The multi-elements composite fertilizer of the present invention has the characteristics of more nutrition, plyability, contained big, middle and trace element with it is micro-
The nutrient that biological nitrogen fixation, potassium decomposing, phosphate solubilizing bacteria are discharged disclosure satisfy that nutritional need of the crop in each growth period, reduce disease pest
Harmful generation, improve the quality and yield of agricultural product.The multi-elements composite fertilizer of the present invention does base manure one-time use, subtracts
Peasant's operating cost is lacked.The multi-elements composite fertilizer of the present invention is green, energy-saving low-carbon, is released in process of production using biology
Technique is put, does not have " three wastes " discharge, fermentation process is carried out under the conditions of normal temperature with thermophilic, without the acid group in chemical fertilizer, alkali
The harmful components such as base, can effectively improved soil and environment.The bar of organic food can be reached using the multi-elements composite fertilizer of the present invention
Part requirement.Show through large area agricultural experiment statistics, increase production using the multi-elements composite fertilizer field crop of the present invention 15%
More than, gourd, fruit and vegetable volume increase more than 30%.
Embodiment
The multielement composite microbe fertilizer of the present invention is with the potassium shale rich in potassium oxide, humic acid and castor rich in organic matter
Numb cake is raw material, the colloid bacillus cereus using deposit number as CGMCCNo.8481（Bacillus mucilaginosus）
Bacillus megaterium (Bacilluss of the HSCUP-76-8 as potassium decomposing strain, using deposit number as CGMCC No.4296
Megaterium) the frankia (Frankia.sp) as phosphorus decomposing strain, using deposit number as CCTCC M207197
Fgc6 bacterial strains after carrying out fermented and cultured to potassium shale and humic acid respectively, are made rich in more based on potassium as fixed nitrogen strain
Elements compounding microorganism fertilizer.
Multielement composite microbe fertilizer of the present invention is to be prepared according to the following steps：
(1) prepare potassium shale powder and choose potassium shale of the potassium oxide content more than 8%, be ground into 150-250 mesh, it is standby；
(2) prepare humic acid powder and take humic acid of the content of organic matter more than 55%, be ground into 100-150 mesh, it is standby；
(3) preparing castor cake powder takes castor cake to be ground into 80-100 mesh, standby；
(4) preparation potassium decomposing fermented liquid, which goes bail for hide, numbers the colloid bacillus cereus HSCUP-76- for being CGMCCNo.8481
8 are used as potassium solubilizing bacteria to carry out high density fermentation preparation, and concrete technology step is：
Colloid bacillus cereus HSCUP-76-8 is inoculated in slant medium by A, and (constituent of slant medium is：Sugarcane
Sugared 5-6g, NaH2PO4 1-1.5g、MgSO4·7H2O 0.3-0.5g、FeCl3 0.004-0.005g、CaCO3 0-0.1g, fine jade
Fat 20-30g, distilled water 1000mL, pH 7.0-7.4) activated, temperature control cultivates 24-48 hours at 29-33 DEG C,
Original bevel seed is obtained, then original bevel seed is inoculated in slant medium, the same terms culture, inclined-plane kind must be produced
Son；
B will produce inclined-plane seed and be inoculated in fluid nutrient medium (constituent of fluid nutrient medium be：Sucrose 5-10g,
NH4Cl 0.5-1g、NaH2PO4 1-1.5g、MgSO4·7H2O 0.5-1g、FeCl3 0.005g、CaCO3 0.1-0.3g, distillation
In water 1000mL, pH 7.0-7.4,20%-45% fluid nutrient medium, shaking table culture temperature are loaded in 200-500mL container
Degree 29-33 DEG C, rotating speed 160-220r/min, incubation time 8-12 hours, obtain seed liquor；
Seed liquor is inoculated in seed tank culture base by C, and (constituent of seed tank culture base is：Sucrose 2-5g, starch
3-10g、(NH4)2SO4 1-2g/ NH4Cl 0.5-1g, yeast extract 1-2g, NaH2PO4 1-1.5g、MgSO4·7H2O 0.5-
1g、FeCl30.005g, distilled water 1000mL, pH 7.0-7.4, coefficient 0.7-0.8,121 DEG C of steam sterilizings 20 minutes,
Inoculum concentration 5%-10%, 29-33 DEG C of cultivation temperature, throughput 1.0：0.8-1.0（v/v·min）, dissolved oxygen is controlled with speed of agitator
Saturation degree DO values 10%-30%, pH control are cultivated 8~12 hours, obtain seeding tank seed liquor 7.2 ± 0.2；
D by seeding tank seed liquor be inoculated in fermentation basal medium (fermentation basal medium constituent be：Sucrose
5g、 (NH4)2SO4 1-2g/ NH4Cl 0.5-1g, yeast extract 1-2g, NaH2PO4 1-2g、MgSO4·7H2O 0.5-1g、
FeCl30.005g, distilled water 1000mL, pH 7.0-7.4；Wherein described sucrose can use corn flour, starch it is any one
The equivalent of kind or more replaces, coefficient 0.7-0.8,121 DEG C of steam sterilizings 20 minutes, inoculum concentration 5%-10%, control parameter：
29-33 DEG C of temperature, pH value 7.0-7.2, throughput 1：0.8-1.2（v/v·min）, dissolved oxygen saturation degree is controlled with speed of agitator
DO value 20%-30%, foam is controlled by defoamer of vegetable oil；Took a sample at interval of 4 hours, make immediately microscopy observation bacterium number and
Thalli morphology, chemical examination sugar, ammoniacal nitrogen, according to the in good time feed supplement of assay, it is 2g/L-3g/L, with NH to control sugared agent amount4Cl is controlled
Nitrogen source 0.5g/L-0.1g/L, 12-14 hours are cultivated, stop mending nitrogen, then adjust control parameter：33-37 DEG C of temperature, pH value
7.2-8.9, throughput 1：1.0-0.8（v/v·min）, with speed of agitator control dissolved oxygen saturation degree DO values 5%~10%, promote
Sporulation, a sample was taken at interval of 4 hours, make microscopy observation thalli morphology immediately, when the thalli morphology 98% in the microscopy visual field
When being gemma above, stop fermented and cultured, gemma number reaches 1.8 × 109 cfu/mL-2.0×109Cfu/mL, gemma conversion ratio
It is standby more than 75%；
(5) preparation phosphorus decomposing fermented liquid, which goes bail for hide, numbers the bacillus megaterium for being CGMCC No.4296 as phosphorus decomposing
Bacterium carries out high density fermentation preparation, and the constituent of phosphorus decomposing bacterium culture medium is：Glucose 8-10g, (NH4)2SO40.5-0.8g,
NaCl 0.3-0.5g, MgSO47H2O 0.3-0.5g, KCl 0.3-0.5g, FeSO47H2O 0.03-0.05g, MnSO4·
4H2O 0.03-0.05g, Ca3(PO4)25-6g, distilled water 1000mL, pH7.0-7.2；Fermentation time 32-48 hours reach 4.5
×109More than cfu/mL, stop fermented and cultured, it is standby；
(6) prepare fixed nitrogen fermented liquid go bail for hide numbering be CCTCC M207197 frankia fgc6 bacterial strains make
For nitrogen-fixing bacteria, fermentation prepares the constituent of the culture medium of high density bacterium solution based on total mass fraction 100%, glucose
0.03%-0.08%, Tween 80 0.003%-0.008%, Sodium Pyruvate 0.03%-0.08%, (NH4) 2SO40.05%-
0.1%, KH2PO4 0.095%, K2HPO4 0.0435%, MgSO47H2O 0.0096%, CaCl2 0.001%, FeSO4
0.00056%, NaEDTA 0.00075%, trace element water-soluble liquid 0.1%, the vitamin aqueous solution 0.1%, surplus are water；Its
In trace element water-soluble liquid form based on total mass fraction 100%, be H3BO3 0.286%, MnCl2 0.181%, ZnSO4
0.022%, CuSO4 0.008%, Na2MoO4 0.0025%, CoCl2 0.0025% and surplus are water；The vitamin aqueous solution
Composition is vitamin V B1 0.001% based on total mass fraction 100%, nicotinic acid 0.005%, biotin 0.0015%, folic acid
0.001%, calcium pantothenate 0.001%, riboflavin 0.001% and surplus are water；After being cultivated 10-15 days at first 28-30 DEG C, then
Culture 72-96 hours obtain the zymotic fluid that dry cell weight is more than 300 μ g/mL at 28-30 DEG C, standby；
(7) it is the ︰ 0.8 of 1 ︰ 1 by the mass volume ratio of potassium page rock powder ︰ potassium decomposing fermented liquid ︰ phosphorus decomposing fermented liquids, (1) is made
Phosphorus decomposing bacterium solution prepared by potassium decomposing bacterium solution prepared by standby potassium shale powder, (4), (5) is mixed evenly, in 34-36 DEG C, humidity
Under the conditions of 65%-75%, aerobic fermentation 7-9 days, then natural air drying, standby；
(8) it is the ︰ 1 of 5-8 ︰ 1 by the mass volume ratio of humic acid powder ︰ castor cake powder ︰ fixed nitrogen fermented liquids, is prepared by (2)
Nitrogen-fixing bacteria prepared by humic acid powder, the castor cake powder of (3) preparation, (7) are mixed evenly, in 28-30 DEG C, humidity 65%-75%
Under the conditions of, aerobic fermentation 10-12 days, then natural air drying, standby；
(9) nitrogen-fixing bacteria humic acid and castor cake powder prepared by the activation potassium shale powder prepared (7) and (8) is in mass ratio
58-63 ︰ 37-42 are well mixed, and are dried below 35 DEG C to moisture and are less than or equal to 8%, as finished product.
Claims (2)
- A kind of 1. multielement composite microbe fertilizer, it is characterized in that by being prepared according to the following steps：(1) potassium shale of the potassium oxide content more than 8% is chosen, 150-250 mesh is ground into, takes 58%- by mass percentage 63% potassium shale, it is standby；(2) humic acid of the content of organic matter more than 55% is taken, 100-150 mesh is ground into, takes 33%-36% by mass percentage Humic acid powder, it is standby；(3) take castor cake to be ground into 80-100 mesh, take 4%-7% castor cake powder by mass percentage, it is standby；(4) the colloid bacillus cereus HSCUP-76-8 that deposit number is CGMCCNo.8481 is taken to carry out high density hair as potassium solubilizing bacteria Prepared by ferment, concrete technology step is：A. colloid bacillus cereus HSCUP-76-8 is inoculated in into slant medium to be activated, temperature Control cultivates 24-48 hours, obtains original bevel seed, then original bevel seed is inoculated in into inclined-plane culture at 29-33 DEG C Base, the same terms culture, inclined-plane seed must be produced；The constituent of described slant medium is：Sucrose 5-6g, NaH2PO4 1-1.5g、MgSO4·7H2O 0.3-0.5g、FeCl3 0.004-0.005g、CaCO30-0.1g, agar 20-30g, distilled water 1000mL, pH 7.0-7.4；B. production inclined-plane seed is inoculated in fluid nutrient medium, loaded in 200-500mL container 20%-45% fluid nutrient medium, 29-33 DEG C of shaking table culture temperature, rotating speed 160-220r/min, incubation time 8-12 hours, Obtain seed liquor；Described fluid nutrient medium：Sucrose 5-10g, NH4Cl 0.5-1g、NaH2PO4 1-1.5g、MgSO4·7H2O 0.5-1g、FeCl3 0.005g、CaCO30.1-0.3g, distilled water 1000mL, pH 7.0-7.4；C. seed liquor is inoculated in kind Sub- tank culture medium, coefficient 0.7-0.8,121 DEG C of steam sterilizings 20 minutes, inoculum concentration 5%-10%, cultivation temperature 29-33 DEG C, throughput 1.0：0.8-1.0v/vmin, existed with speed of agitator control dissolved oxygen saturation degree DO values 10%-30%, pH control 7.2 ± 0.2, cultivate 8~12 hours, obtain seeding tank seed liquor；The constituent of described seed tank culture base is：Sucrose 2- 5g, starch 3-10g, (NH4)2SO4 1-2g、NH4Cl 0.5-1g, yeast extract 1-2g, NaH2PO4 1-1.5g、MgSO4·7H2O 0.5-1g、FeCl30.005g, distilled water 1000mL, pH 7.0-7.4；D. seeding tank seed liquor is inoculated in the training of fermentation basis Support in base, coefficient 0.7-0.8,121 DEG C of steam sterilizings 20 minutes, inoculum concentration 5%-10%, control parameter：Temperature 29-33 DEG C, pH value 7.0-7.2, throughput 1：0.8-1.2v/vmin, dissolved oxygen saturation degree DO value 20%-30% are controlled with speed of agitator, Foam is controlled by defoamer of vegetable oil；A sample was taken at interval of 4 hours, makees microscopy observation bacterium number and thalli morphology immediately, changes Test sugar, ammoniacal nitrogen；According to the in good time feed supplement of assay, it is 2g/L-3g/L, with NH to control sugared agent amount4Cl control nitrogen sources 0.5g/L- 0.1g/L, 12-14 hours are cultivated, stop mending nitrogen, then adjust control parameter：33-37 DEG C of temperature, pH value 7.2-8.9, throughput For 1：1.0-0.8v/vmin, with speed of agitator control dissolved oxygen saturation degree DO values 5%~10%, promote sporulation, at interval of A sample is taken within 4 hours, makees microscopy observation thalli morphology immediately, when the thalli morphology more than 98% in the microscopy visual field is gemma, Stop fermented and cultured, gemma number reaches 1.8 × 109cfu/mL-2.0×109Cfu/mL, gemma conversion ratio are standby more than 75% With；The constituent of described fermentation basal medium is：Sucrose 5g, (NH4)2SO4 1-2g、NH4Cl 0.5-1g, yeast leaching Powder 1-2g, NaH2PO4 1-2g、MgSO4·7H2O 0.5-1g、FeCl30.005g, distilled water 1000mL, pH7.0-7.4；(5) bacillus megaterium that deposit number is CGMCC No.4296 is taken to carry out high density fermentation preparation, solution as phosphate solubilizing bacteria The constituent of phosphorus bacterium culture medium is：Glucose 8-10g, (NH4)2SO40.5-0.8g, NaCl 0.3-0.5g, MgSO4·7H2O 0.3-0.5g, KCl 0.3-0.5g, FeSO4·7H2O 0.03-0.05g, MnSO4·4H2O 0.03-0.05g, Ca3(PO4)2 5-6g, distilled water 1000mL, pH7.0-7.2；Fermentation time 32-48 hours reach 4.5 × 109More than cfu/mL, stop fermentation Culture, it is standby；(6) potassium shale powder is pressed：Potassium decomposing bacterium solution：The mass volume ratio of phosphorus decomposing bacterium solution is 1：1：0.8, by (1) prepare potassium shale powder, (4) potassium decomposing bacterium solution, the phosphorus decomposing bacterium solution of (5) preparation prepared is mixed evenly, in 34-36 DEG C, humidity 65%-75% conditions Under, aerobic fermentation 7-9 days, then natural air drying, standby；(7) the frankia fgc6 bacterial strains that deposit number is CCTCC M207197 are taken to prepare high density as nitrogen-fixing bacteria, fermentation The constituent of the culture medium of bacterium solution is based on total mass fraction 100%, glucose 0.03%-0.08%, Tween 80 0.003%- 0.008%, Sodium Pyruvate 0.03%-0.08%, (NH4)2SO40.05%-0.1%, KH2PO40.095%, K2HPO4 0.0435%, MgSO4·7H2O 0.0096%, CaCl20.001%, FeSO40.00056%, NaEDTA 0.00075%, Trace element water-soluble liquid 0.1%, the vitamin aqueous solution 0.1%, surplus are water；Trace element water-soluble liquid composition therein presses total matter Amount fraction 100% is counted, and is H3BO30.286%, MnCl20.181%, ZnSO40.022%, CuSO40.008%, Na2MoO4 0.0025%, CoCl20.0025% and surplus be water；The vitamin aqueous solution is formed based on total mass fraction 100%, is given birth to for dimension Plain VB10.001%, nicotinic acid 0.005%, biotin 0.0015%, folic acid 0.001%, calcium pantothenate 0.001%, riboflavin 0.001% and surplus be water；After being cultivated 10-15 days at first 28-30 DEG C, then culture 72-96 hours obtain thalline at 28-30 DEG C Dry weight is more than 300 μ g/mL zymotic fluid, standby；(8) castor cake powder is added by humic acid powder：The mass volume ratio of nitrogen-fixing bacteria is 1：1, humic acid powder, (3) system prepared by (2) Nitrogen-fixing bacteria prepared by standby castor cake powder, (7) are mixed evenly, under the conditions of 28-30 DEG C, humidity 65%-75%, aerobic hair Ferment 10-12 days, then natural air drying, standby；(9) nitrogen-fixing bacteria humic acid and castor cake powder prepared by the activation potassium shale powder prepared (6) and (8) is well mixed, 35 Dried below DEG C to moisture and be less than or equal to 8%, as finished product.
- 2. a kind of multielement composite microbe fertilizer as claimed in claim 1, it is characterised in that in (4), described sucrose is beautiful Any one of ground rice, starch or more equivalent replace.
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| CN104446694A (en) * | 2014-11-25 | 2015-03-25 | 张吉科 | Frankia super bacterial manure fermenting technology |
| CN106748257A (en) * | 2017-01-17 | 2017-05-31 | 山东象力丰生物科技有限公司 | A kind of nitrogen-fixing microorganism Chemical Mixed Fertilizer and preparation method |
| CN106699474A (en) * | 2017-02-23 | 2017-05-24 | 胡世洋 | Novel bio-organic fertilizer and preparation method thereof |
| CN107400640A (en) * | 2017-07-11 | 2017-11-28 | 北京航天恒丰科技股份有限公司 | A kind of soil conditioner containing potassium solubilizing bacteria and preparation method thereof |
| JP7011238B2 (en) * | 2017-09-29 | 2022-02-10 | 日清ファルマ株式会社 | Highly efficient production method of alkylresorcinol using microorganisms |
| CN108409417A (en) * | 2018-04-16 | 2018-08-17 | 中科国思生物科技研究（广州）有限公司 | A kind of microbial manure prepared by frankia |
| CN110981591A (en) * | 2019-12-20 | 2020-04-10 | 海南科大林业有限公司 | Special growth-promoting fertilizer for oil tea, and preparation and application thereof |
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| CN102826895B (en) * | 2012-08-01 | 2014-04-23 | 邓振山 | Multi-element composite microorganism bacterial manure and preparation method thereof |
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| CN1580007A (en) * | 2004-05-14 | 2005-02-16 | 张自恒 | Multi-element organic nutrient fertilizer and its preparing method |
| CN101229986A (en) * | 2007-12-29 | 2008-07-30 | 中国林业科学研究院热带林业研究所 | Microbial fertilizer specially for beef-wood |
| CN101475417A (en) * | 2009-01-20 | 2009-07-08 | 牧耀贵 | Biological mineral compound fertilizer and production method thereof |
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