CN100399906C

CN100399906C - Carposome extract used for inducing plant disease resistance

Info

Publication number: CN100399906C
Application number: CNB011306017A
Authority: CN
Inventors: 齐鸿雁; 白志辉; 张洪勋; 李宝聚
Original assignee: Research Center for Eco Environmental Sciences of CAS
Current assignee: Research Center for Eco Environmental Sciences of CAS
Priority date: 2001-08-15
Filing date: 2001-08-15
Publication date: 2008-07-09
Anticipated expiration: 2021-08-15
Also published as: CN1345540A

Abstract

The present invention relates to a method for extracting polysaccharide from agaricus blazei as a fungus, and the effect of the polysaccharide of resisting plant diseases is tested. A preparation method mainly comprises the steps of raw material pulverization, extraction by hot water, pressure reduction concentration, precipitation by ethanol, drying, etc. The use formulation of prepared polysaccharide is an aqueous agent which is aided with silicone oil as a penetrating agent to be sprayed on a cucumber plant and a tomato plant during a growth period so as to induce and activate the disease resistance and defense system of the plants, so that the aim of preventing and curing diseases are achieved. The present invention is used for resisting diseases of downy mildew, anthracnose, powdery mildew, scab and sphaceloma arallae.

Description

A kind of fungus sporophore extract is used for the inducing plant disease resistance

Fungi Agaricus blazei Murrill (Agaricus blazei) fruiting body extract is a kind of low toxin preparation as plant disease resistance inductor, and its disease-resistant principle is to induce the also disease-resistant system of defense of activated plant self.Direct purpose of the present invention provides safety, hypotoxic disease-resistant agricultural chemicals.

Fungus sporophore is from commodity, obtain extract through following method: fungus sporophore is through 80 ℃ of dryings, mechanical crushing to 40 order, boiling water refluxing extraction 2 hours, filter and remove residue, supernatant is evaporated to total sugar concentration and is about 30% (w/v), add 95% precipitation with alcohol, filter, precipitation is dry, obtain powder, ethanol distillation reclaims.The goods that obtain with the method can directly use as plant disease resistance inductor.Active ingredient is fungi polysaccharide and oligosaccharides.Said preparation uses formulation to be the aqueous solution, will add solvent, penetrant, wetting agent and stabilizing agent etc. in case of necessity, makes the wettable powder type.

When using the wettable powder type, working concentration calculates with solids content, generally is advisable with 0.1-1g/L, and every mu of vegetables applied once 60-75L sprayed once at interval in 3-5 days, and according to the application target difference, working concentration and sprinkling number of times can suitably be adjusted.

The surfactant that uses as solvent, penetrant and wetting agent has the sulfuric ester, high-grade aliphatic ester, alkyl aryl sulfonate, epoxides of higher alcohol etc.

Fungal extract of the present invention to the oral toxicity of mouse extremely a little less than, and contrast almost indifference, experimental result is as follows:

Tried thing: being tried thing is the fungus sporophore solution of extract, and it is liquid to be light coffee color, the about 1.0g/ml of proportion, and concentration is 1mg/ml.

Animal: healthy Kunming kind small white mouse, body weight 20-23 gram is provided by the department of the Chinese Academy of Sciences of laboratory animal section of Beijing Medical University, the quality certification number: the moving word 01-3049 of doctor.

Through trial test, it is very low to be tried thing toxicity, so according to the dosage of 0.2ml/10g body weight, it is the 20g/kg body weight that poisoning dosage is set.Animal is divided into two groups at random, and 10 every group, male and female half and half are respectively control group and experimental group.After the animal fasting 8 hours, the experimental group animal is once irritated stomach with 0.2ml/10g body weight per os and is tried thing.Control animals is irritated stomach and is given distilled water.Observe toxic reaction symptom and the weight of animals variation after the administration, observed continuously 14 days.

Result of the test: animal does not see the overt toxicity reaction symptom after irritating the stomach contamination.Female contamination treated animal body weight and control group relatively decrease, and buck body weight change difference does not have significance.Do not find animal dead in observation period.Postmortem behind the sacrifice of animal, each internal organs do not see that overt toxicity changes.

Conclusion: fungus sporophore extract its mouse oral is irritated the stomach maximal tolerance dose more than the 20g/kg body weight.The per os acute toxicity belongs to low toxicity.

Embodiment:

1. downy mildew resistance: fungus sporophore extract (hereinafter to be referred as disease-resistant derivant) is made concentration 0.6mg/ml aqua and is aided with bleeding agent silicone oil, after treating that cucumber seedling grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval, in contrast with the plant of spraying clear water at every turn.In same time inoculation bacterium of downy mildew of cucumber, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 33.4-49.5%, and the disease-resistant lasting period is more than 6 days.

2. anti-anthracnose: fungus sporophore extract (hereinafter to be referred as disease-resistant derivant) is made concentration to be the 0.6mg/ml aqua and to be aided with bleeding agent silicone oil, after treating that cucumber seedling grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval, in contrast with the plant of spraying clear water at every turn.In same time inoculation cucumber anthracnose, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 43.6-54.8%.

3. mildew-resistance: fungus sporophore extract (hereinafter to be referred as disease-resistant derivant) is made concentration to be the 0.6mg/ml aqua and to be aided with bleeding agent silicone oil, after treating that cucumber seedling grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval, in contrast with the plant of spraying clear water at every turn.In same time inoculation powdery mildew of cucumber bacterium, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 31.8-34.6%.

4. scab resistant: fungus sporophore extract (hereinafter to be referred as disease-resistant derivant) is made concentration to be the 0.6mg/ml aqua and to be aided with bleeding agent silicone oil, after treating that cucumber seedling grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval, in contrast with the plant of spraying clear water at every turn.In same time inoculation dosporium cucumerinumand its, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 56.8-78.4%.

5. anti-shot hole: fungus sporophore extract (hereinafter to be referred as disease-resistant derivant) is made concentration to be the 0.6mg/ml aqua and to be aided with bleeding agent silicone oil, after treating that tomato seedling grows five compound leaves, spray disease-resistant derivant 2 times, induced 3 days at interval, in contrast with the plant of spraying clear water.In same time inoculation Streptomyces scabies, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 70.92-74.73%.

The bacterial classification explanation:

Strain name: Agaricus blazei Murrill (Agaricus blazei); China Committee for Culture Collection of Microorganisms registers on the books at the common micro-organisms center and is numbered: CGMCC No.0567.

Claims

1. one kind with the prepared plant disease resistance inductor of the method that comprises the steps, it is characterized in that the step of described method comprises:

1) fungi Agaricus blazei Murrill (Agaricus blazei) fruit body is also pulverized 80 ℃ of dryings;

2) the boiling water refluxing extraction is 2 hours;

3) filter cleaner, supernatant are evaporated to total sugar concentration and are about 30% according to percent weight in volume;

4) 95% precipitation with alcohol;

5) filter and will precipitate drying.

2. the described plant disease resistance inductor of claim 1 is characterized in that described derivant is a pulvis.

3. the described plant disease resistance inductor of claim 1, the formulation that it is characterized in that described derivant is an aqua.

4. the purposes of each described plant disease resistance inductor of claim 1-3 in inducing plant antimycotic and bacteriosis.

5. the described purposes of claim 4, wherein said mycosis is a Pseudoperonospora cubensis, white powder germ, anthrax bacteria, the microbial phytopathy of scab.

6. the described purposes of claim 4, wherein said bacteriosis is the caused phytopathy of Streptomyces scabies.

7. the described purposes of claim 4, wherein said plant is cucumber or tomato.