CN1112104C - Vegetable disease preventing and treating prepn. and its usage - Google Patents
Vegetable disease preventing and treating prepn. and its usage Download PDFInfo
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- CN1112104C CN1112104C CN 00129853 CN00129853A CN1112104C CN 1112104 C CN1112104 C CN 1112104C CN 00129853 CN00129853 CN 00129853 CN 00129853 A CN00129853 A CN 00129853A CN 1112104 C CN1112104 C CN 1112104C
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- polygalacturonase
- scab
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- anthracnose
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Abstract
The present invention provides a preparation for inducing and activating self defense systems of cucumbers in a growth period to prevent downy mildew, gray mold, anthracnose, scabs, powdery mildew, corky scabs and angular leaf spots, which is characterized in that the preparation is an aqueous solution containing polygalacturonase and penetrating agents. The dosage of the penetrating agents is from one in hundred thousand to one in two thousand of the total weight of the aqueous solution, the specific activity of the polygalacturonase is 50 unit/milligram protein, and the use concentration of the polygalacturonase is from 40 to 100 microgram protein/milliliter. The present invention also provides a method for using the preparation, which comprises: 60 to 75L is applied to vegetables in each mu once and the preparation is sprayed once every three to five days. The preparation of the present invention has good effect on prevention and treatment.
Description
Technical field
The present invention relates to a kind of preparation and using method thereof of preventing and treating vegetable disease, specifically, thereby the present invention relates to a kind of preparation and using method thereof that vegetables self-defense system prevents and treats disease of inducing and activate.
Technical background
The plant pathology result of study shows that plant itself has disease-resistant " defence " or " immunity " system.Usually, this " defence " or " immunity " system need the experience certain hour could activate after phytopathogen invasion, promptly enable the new pathogen of impedance at this moment and invade, but plant suffers damage, and the fruit underproduction is can hardly be avoided.The biochemistry reaction that takes place behind the instruction plant by the research pathogen, find that the cell wall of plant produces the oligosaccharide of different polymerization degree under the effect of polygalacturonase, and then the synthetic protective plant protecting agent of oligosaccharide inducing plant tissue, finally produce disease resisting effect.Obviously, polygalacturonase is the important component in above-mentioned a series of biochemical reactions.
This invention is according to above-mentioned principle, utilize the polygalacturonase of fermentation method preparation, its preparation method is referring to (Hongxun Zhang, Maojie Xue, Deming Liu and Hongyan Qi, APilot Plant for Production of Pectic Enzyme by Solid State Fermentation, " Better Living Through Innovative Biochemical Engineering " W.K.Teo, M.G.S.Yap and S.K.W.Oh (Eds), 1994, p101-103) spray cucumber and the tomato of having in one-tenth, inducing plant produces protective plant protecting agent, has produced protection effect.This method is domestic does not appear in the newspapers as yet, though foreign literature has report (1.Mary walker-Simmons, Lee Hadwiger andClarence A.Byan, Chitosans and pectic polysaccharides both induce theaccumulation of the antifungal phytoalexin in pea pods and antinutrientproteinase inhibitors in tomato leaves.Biochemical and biophysical researchcommunications, Vo1.110 (1), 1983; 2.Eugene.A.Nothnagel, Michael Mcnell, Peter Albersheim and Anne Dell, Host-pathogen interaction, Plant physiology, Vo1.71,916-926,1993), but mainly aspect theory research, there is no result of practical application.
Polygalacturonase is a kind of low toxin preparation as plant disease resistance inductor, and its disease-resistant principle is to induce the also disease-resistant system of defense of activated plant self.Direct purpose of the present invention provides safety, hypotoxic disease-resistant agricultural chemicals.
Summary of the invention
Thereby an object of the present invention is to provide a kind of cucumber and tomato self-defense system that induces and activate vegetative period and prevent and treat the preparation of downy mildew, gray mold, anthracnose, scab, powdery mildew and scab disease.
Thereby another object of the present invention provides a kind of method of using cucumber that preparation of the present invention induced and activated vegetative period and tomato self-defense system to prevent and treat downy mildew, gray mold, anthracnose, scab, powdery mildew, shot hole and angular leaf spot.
Thereby the invention provides a kind of cucumber and tomato self-defense system that induces and activate vegetative period and prevent and treat the preparation of downy mildew, gray mold, anthracnose, scab, powdery mildew and scab disease, it is characterized in that, said preparation is the aqueous solution that contains polygalacturonase and bleeding agent, the consumption of bleeding agent be this aqueous solution gross weight 100,000/to ten thousand/, described polygalacturonase is 50-60 unit/milligram albumen than vigor, and working concentration is 40-100 micrograms of protein/milliliter.
In above-mentioned preparation of the present invention, described bleeding agent can use any bleeding agent well known in the prior art, as silicone oil.
Thereby the present invention also provides a kind of method of using cucumber that above-mentioned preparation of the present invention induced and activated vegetative period and tomato self-defense system to prevent and treat downy mildew, gray mold, anthracnose, scab, powdery mildew, shot hole and angular leaf spot, comprise the amount that described preparation is risen with every mu of vegetables applied once 60-75, for downy mildew, gray mold, anthracnose, scab, powdery mildew, sprayed once in 2 days at interval; For shot hole, sprayed once in 3-7 days at interval, for angular leaf spot, 7-15 sprays once at interval.
Polygalacturonase obtains (Hongxun Zhang from microbial fermentation, Maojie Xue, Deming Liu and Hongyan Qi, A Pilot Plant for Production of Pectic Enzymeby Solid State Fermentation, " Better Living Through Innovative BiochenicalEngineering " W.K.Teo, M.G.S.Yap and S.K.W.Oh (Eds), 1994, p101-103), the product that obtains with the method can directly use as plant disease resistance inductor.Common described active ingredient polygalacturonase is the solution of solvent with water, will add solvent, penetrant, wetting agent and stabilizing agent etc. in case of necessity, makes the wettable powder type.
According to the application target difference, working concentration and sprinkling number of times can suitably be adjusted.Protein concentration is measured (" biochemical test method and technology ", Zhang Longxiang, Zhang Tingfang and Li Lingyuan chief editor, Higher Education Publishing House's publication, 164 pages, 1987) with the biuret method.Enzyme activity is defined as: under 40 ℃, pH4.2 condition, the enzyme amount that made 1 milligram of pectin come unstuck required fully in 30 minutes is unit of activity (list of references Friedrich, a Jcimerman, A, et al., Applied microbiology andbiotechnology, 31,490 (1989)).The method principle is: pectin substance (main component is a polygalacturonic acid) is the sugar chain fracture under the effect of polygalacturonase, the degree of fracture is directly proportional with enzymic activity, by regularly adding the boundary point (terminal point promptly comes unstuck) that isopropyl alcohol observing response liquid generates precipitation, estimate enzymic activity according to usually time length.
The surfactant that uses as solvent, penetrant and wetting agent has the sulfuric ester, high-grade aliphatic ester, alkyl aryl sulfonate, epoxides of higher alcohol etc.
Disease-resistant derivant agricultural chemicals of the present invention to the oral toxicity of mouse extremely a little less than, and contrast almost indifference, experimental result is as follows:
Tried thing: being tried thing is active ingredient polygalacturonase and this enzyme catabolite oligogalacturonans aqueous solution thereof, and protein concentration is 6mg/ml, the about 1.0g/ml of proportion.
Animal: healthy Kunming kind small white mouse, body weight 20-23 gram; Provide the quality certification number by the department of the Chinese Academy of Sciences of laboratory animal section of Beijing Medical University: the moving word 01-3049 of doctor.
Test method: through trial test, it is very low to be tried thing toxicity, so according to the dosage of 0.2ml/10g body weight, it is the 20g/kg body weight that poisoning dosage is set.Animal is divided into two groups at random, and 10 every group, male and female half and half are respectively control group and experimental group.After the animal fasting 8 hours, the experimental group animal is once irritated stomach with 0.2ml/10g body weight per os and is tried thing.Control animals is irritated stomach and is given distilled water.Observe toxic reaction symptom and the weight of animals variation after the administration, observed continuously 14 days.
Result of the test: animal does not see the overt toxicity reaction symptom after irritating the stomach contamination.Contamination group contamination back different time the weight of animals and control group compare difference, and there are no significant.Do not find animal dead in observation period.Postmortem behind the sacrifice of animal, each internal organs do not see that overt toxicity changes.Conclusion: its mouse oral is irritated the stomach maximal tolerance dose and (be equivalent to about 26 M more than the 20g/kg body weight
2Usage amount of vegetables), the per os acute toxicity belongs to low toxicity.
Embodiment:
1. downy mildew resistance: it is 40 μ g/ml that polygalacturonase (hereinafter to be referred as disease-resistant derivant) is diluted to protein concentration, after treating that cucumber grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval at every turn, with the plant of spraying clear water in contrast, in same time inoculation bacterium of downy mildew of cucumber, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect (" preventive effect " is called for short for control efficiency) increases according to spraying number of times, scope is 47.85-69.78%, and the disease-resistant lasting period is more than 6 days.
2. anti-anthracnose: it is 40 μ g/ml that polygalacturonase (hereinafter to be referred as disease-resistant derivant) is diluted to protein concentration, after treating that cucumber grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval at every turn, with the plant of spraying clear water in contrast, in same time inoculation cucumber anthracnose, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 24.22-69.69%.
3. mildew-resistance: it is 40 μ g/ml that polygalacturonase (hereinafter to be referred as disease-resistant derivant) is diluted to protein concentration, after treating that cucumber grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval at every turn, with the plant of spraying clear water in contrast, in same time inoculation powdery mildew of cucumber bacterium, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 43.63-49.22%.
4. scab resistant: it is 40 μ g/ml that polygalacturonase (hereinafter to be referred as disease-resistant derivant) is diluted to protein concentration, after treating that cucumber grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval at every turn, with the plant of spraying clear water in contrast, in same time inoculation dosporium cucumerinumand its, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 20.96-36.85%.
5. botrytis resistant: it is 40 μ g/ml that polygalacturonase (hereinafter to be referred as disease-resistant derivant) is diluted to protein concentration, after treating that tomato grows six true leaves, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval at every turn, with the plant of spraying clear water in contrast, in same time inoculating tomato ash arrhizus bacteria, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 37.26-68.73%.
6. anti-shot hole: it is 40 μ g/ml that polygalacturonase (hereinafter to be referred as disease-resistant derivant) is diluted to protein concentration, after treating that tomato grows six true leaves, difference interval 3, sprayed disease-resistant derivant in 5,7 days, in contrast with the plant of spraying clear water, in same time inoculating tomato Streptomyces scabies, begin the classification investigation when spraying the adjoining tree morbidity of clear water, preventive effect increases and reduces according to spraying interval, and scope is 51.44-70.89%.
7. anti-angular leaf spot: it is 40 μ g/ml that polygalacturonase (hereinafter to be referred as disease-resistant derivant) is diluted to protein concentration, after treating the long field planting of cucumber, sprayed disease-resistant derivant respectively at interval in 7,15 days, with the plant of spraying clear water in contrast, in same time inoculation angular leaf spot of cucumber bacterium, in the investigation of the Sheng phase as a result state of an illness, preventive effect is respectively 66.79 and 48.70%.
Claims (3)
1. thereby a cucumber and a tomato self-defense system that induces and activate vegetative period prevents and treats the preparation of downy mildew, gray mold, anthracnose, scab, powdery mildew and scab disease, it is characterized in that, said preparation is the aqueous solution that contains polygalacturonase and bleeding agent, the consumption of bleeding agent be this aqueous solution gross weight 100,000/to ten thousand/, described polygalacturonase is 50-60 unit/milligram albumen than vigor, and working concentration is 40-100 micrograms of protein/milliliter.
2. according to the described preparation of claim 1, wherein, described bleeding agent is a silicone oil.
3. thereby method of using cucumber that the described preparation of claim 1 induced and activated vegetative period and tomato self-defense system to prevent and treat downy mildew, gray mold, anthracnose, scab, powdery mildew, shot hole and angular leaf spot, comprise the amount that described preparation is risen with every mu of vegetables applied once 60-75, for downy mildew, gray mold, anthracnose, scab, powdery mildew, sprayed once in 2 days at interval; For shot hole, sprayed once in 3-7 days at interval, for angular leaf spot, 7-15 sprays once at interval.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00129853 CN1112104C (en) | 2000-10-20 | 2000-10-20 | Vegetable disease preventing and treating prepn. and its usage |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00129853 CN1112104C (en) | 2000-10-20 | 2000-10-20 | Vegetable disease preventing and treating prepn. and its usage |
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| Publication Number | Publication Date |
|---|---|
| CN1349741A CN1349741A (en) | 2002-05-22 |
| CN1112104C true CN1112104C (en) | 2003-06-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 00129853 Expired - Fee Related CN1112104C (en) | 2000-10-20 | 2000-10-20 | Vegetable disease preventing and treating prepn. and its usage |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585247A (en) * | 2015-02-13 | 2015-05-06 | 陆青 | Wettable powder for preventing and controlling Chinese cabbage soft rot |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283538B (en) * | 2013-05-30 | 2014-07-23 | 尉晨 | Protected-field overwintering tomato disease control method |
| CN105123722A (en) * | 2015-08-03 | 2015-12-09 | 佛山市顺德区宝铜金属科技有限公司 | Composition for seed treatment |
| CN105767004A (en) * | 2016-04-08 | 2016-07-20 | 广西南宁益土生物科技有限责任公司 | Peanut ralstonia solanacearum inhibitor prepared from lactose |
| CN105766914A (en) * | 2016-04-08 | 2016-07-20 | 广西南宁益土生物科技有限责任公司 | Method for preventing and treating peanut bacterial wilt with lactose high-temperature processing product |
| CN105766913A (en) * | 2016-04-08 | 2016-07-20 | 广西南宁益土生物科技有限责任公司 | Application of lactose high-temperature treatment product to peanut ralstonia solancearum inhibition |
-
2000
- 2000-10-20 CN CN 00129853 patent/CN1112104C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585247A (en) * | 2015-02-13 | 2015-05-06 | 陆青 | Wettable powder for preventing and controlling Chinese cabbage soft rot |
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| Publication number | Publication date |
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| CN1349741A (en) | 2002-05-22 |
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