KR20060107521A - Composition having plant disease resistance inducing activity and process for producing the same - Google Patents

Abstract

A plant disease resistance inducing composition realizing reduction of environmental load and exhibiting excellent control activity, which composition comprises an amphiphile derived from plant raw materials as an active ingredient. There is further provided a process for producing the same.

Description

Composition having plant disease resistance inducing activity and process for producing the same

The present invention relates to a plant disease resistant induction composition having a low environmental load and having an excellent control action, and a method for producing the same.

Chemical pesticides such as fungicides and invasive inhibitors are mainly used in the control of crop diseases.These chemical pesticides include chemicals having a bactericidal activity against plant pathogens or agents that inhibit the infectious function of pathogens. Doing. However, these types of pesticides are considered to have a significant impact on not only target organisms, but there are concerns about safety for users, effects on crop safety due to residues in crops, and environmental loads. Moreover, it is also clear that by continuing to use such chemical pesticides, the generation of resistant bacteria to drugs is promoted.

In addition, soil disinfectants are used for soil diseases caused by soil infectious pathogens, because they do not work as a disinfectant against pathogens that live in the soil. The use of these soil disinfectants causes serious environmental problems than disinfectants, is concerned with the fact that they have a greater effect on the safety of the human body, and the like, and is also known to be related to a decrease in intellect, because it kills useful soil bacteria.

For this reason, it is required to establish a continuous agricultural production technology utilizing natural circulation without excessively depending on chemical pesticides and the like. In order to achieve this objective, the development of pest control agents in harmony with the environment is under consideration. In addition, one of agricultural materials considered to be an environmentally harmonious pest control agent includes a pesticide (resistance-inducing pesticide) of a type which is controlled by increasing resistance to the disease inherent in plants. This resistance-induced pesticide is estimated to have little impact on the environment, including pathogens and other organisms. Examples of resistance-induced pesticides that have been put to practical use include furobenazole, bione, and thiadinil, all of which are registered as pesticides in Japan as rice blast control agents. In addition, since these resistance-inducing pesticides do not directly act against plant pathogens, the emergence of resistant bacteria to these drugs has not been reported so far, and it is thought that there is little possibility of problems with drug resistance in the future. In addition, since the resistance of a plant to a disease is basically common except for some of the diseases, crops in which resistance is induced by resistance inducing agents have a feature of controlling a plurality of diseases.

In addition to the above-described practical resistance-inducing pesticides, substances which induce disease resistance of plants include polysaccharide degradants which induce white alexin (JP-A-5-331016) and derivatives of jasmonic acid (JP-A-11-). 29412), salicylic acid (Delaney TP, Science, 1994, 266, 1247-1250), arachidonic acid (Bostock M. Science, 1981, 212, 349-360), and the like. In addition, as a plant-derived ingredient, a combination of rice bran- derived ingredients with vinegar (Japanese Patent Laid-Open No. 2001-61344) and soy oil (Japanese Patent Laid-Open No. 2002-293709) induce disease resistance. Known. On the other hand, in order to exert a practical control effect against a disease, it is also known that it is not enough to activate a part of the disease resistance reaction by the resistance inducing substance.

In addition, it is known that cerebrosides derived from filamentous fungi exhibit a disease resistance induction effect against rice such as rice blasting (Japanese Patent No. 2846610, W098 / 47364, WO03 / 020032). ). Selebrosides are a kind of spino lipids, compounds in which monosaccharides are glycosidically bonded to the primary alcohols of ceramides. Spinolipids are commonly present as membrane components in many species, but they have been shown to be selbrosides with a 9th-methylated branch, which is characteristic of filamentous fungi. Koga J. et al. J. Biol. Chem., 1998, 48 (27), 31985-31991, Umemura K. et al. Plant Cell Physiol., 2000, 41 (6), 676-683).

As a disease control effect by selbrosides, the rice disease control effect (the Japanese Patent No. 2846610, W098 / 47364) by cerebroside derived from a plant pathogen, and the selbroside derived from filamentous bacteria Disease control against various crops is known (W003 / 020032). These are all active ingredients of cerebrosides with the 9th methyl branch, which is unique to filamentous fungi, including plant pathogens.

On the other hand, it is known that selbroside having a spinocin base moiety is composed of various molecular species in celebromide derived from plants (Kaul, K. et aI., Plant Physiol., 1975, 55, 120-). 129, Ohnishi M. et aI., Biochem.Biophys.Acta, 1983, 752, 416-422, Imai H. et aI., Biosci.Biotechnol.Biochem., 1997,61,351-353, Imai H. et aI., Plant Physiol., 2000, 157, 453-456.

In addition, the physiological function of spino lipids derived from plants has not yet been elucidated, but at low temperature (Uemura, M. et aI., Plant Physiol., 1994, 104, 479-496, Uemura, M). et aI., Plant Physiol., 1995, 109, 15-30) or dry stress (Norverg, N. et aI., Biochim. Biophys. Acta, 1991, 1066, 257-260), fruiting bodies Induction (Kawai, G. et al., J. Biol. Chem., 1986, 261, 779-784), control of the opening and closing of pores (Ng C. et aI., Nature, 2001, 410, 596-599, Coursol S. et aI., Nature, 2003, 423, 651-653). On the other hand, there have been no reports of plant-derived spino lipids associated with disease resistance.

In addition, as consumer demand for food safety increases, demand for organic products grown without using chemically synthesized pesticides is increasing. In addition, conventional resistance-induced pesticides that are considered to be less environmentally friendly are chemically synthesized pesticides and cannot be used for cultivating organic products. On the other hand, according to the guidelines announced by the Ministry of Agriculture, Forestry and Fisheries, natural useful mineral materials, plants, animals, and natural substances extracted, extracted or prepared from them are required to be registered as pesticides under the Pesticide Regulation Act. It can be used as pesticides such as dragons. Therefore, in order to provide agricultural materials that are safe for users and consumers and can be used for organic farming, etc., it is desired to produce a resistance-derived control agent derived from natural products.

The present inventors have found out that the amphipathic substances contained in plants have excellent plant disease resistance inducing activity, and thus have remarkable control against various plant diseases. This invention is based on this knowledge.

Therefore, an object of the present invention is to provide a plant disease resistant induction composition and a method of manufacturing the same.

The plant disease resistant induction composition according to the present invention contains an amphiphilic substance derived from a plant as an active ingredient.

In addition, the method for producing a plant disease resistant inducible composition according to the present invention comprises extracting an amphiphilic substance from a plant raw material with a mixed solvent or an organic solvent of two or more substances selected from the group consisting of water, a surfactant and an organic solvent. Concentrating the extract obtained by extraction, and depositing and removing the physiologically harmful substance of the plant contained in an extract.

The plant disease resistant inducible composition according to the present invention uses a plant or a plant processed product as its raw material, and thus can be usefully used as a safe pesticide for producers and consumers of agricultural products. In addition, the plant disease resistant induction composition according to the present invention is an environmentally harmonized agricultural material that can be used for continuous agricultural production technology or organic cultivation.

Next, the present invention will be described in more detail.

In the present invention, the term "amphiphilic substance" refers to a molecule having both a polar group and a hydrophobic group, which means that the hydrophobic group is collected by hydrophobic bonds at a critical micelle concentration, so that the solubility in water is improved by forming a micelle in which the polar group becomes a surface. It is characterized by one. In addition, in this invention, "simple lipid" means ester of a fatty acid and various alcohols. In addition, in this invention, "composited lipid" means the lipid which contains P (phosphorus), S (sulfur), a nitrogen-containing base, or a sugar in a molecule | numerator. In addition, in this invention, "phosphorus lipid" means the lipid which has a phosphate ester or a phosphonic acid ester in a molecule | numerator. In addition, in this invention, a "sugar lipid" means the lipid which has sugar in a molecule | numerator. In the present invention, "spingo lipid" refers to a lipid containing a long chain base including spinocin, wherein the chain length is preferably 14 to 24 carbon atoms, more preferably. The following is 18. In addition, in this invention, a "glycero lipid" means the lipid containing glycerol.

The disease resistant induction composition according to the present invention is characterized by comprising an amphiphilic substance derived from a plant as an active ingredient. The amphiphilic substance in the present invention may contain a simple lipid or a composite lipid alone or in combination. Thus, according to a preferred aspect of the present invention, the amphipathic material is a simple lipid, a composite lipid or a mixture thereof.

Simple lipids in the present invention are preferably triglycerides, diglycerides, or monoglycerides, and more preferably diacylglycerol.

In addition, the composite lipid in the present invention is preferably phospholipid and / or glycolipid. And the phosphorus lipid is preferably phospholipid or spinophospholipid with glycerol, more preferably phosphatidylcholine. In addition, the sugar lipid is preferably glycerol glycolipid or spin high sugar lipid, more preferably selbroside.

Moreover, according to one aspect of the present invention, the composite lipid is spino lipid. Preferred examples of such spinolipids include the following compounds:

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxypalmitriyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxyvaseyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-4-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-4-hydroxy-spinganine,

1- O- β-glucosyl-N-2'-hydroxypalmitriyl-4-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-4-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-4-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxyvaseyl-4-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-4-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-4-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-4-hydroxy-spinganine

1- O- β-glucosyl- N- 2'-hydroxybehenyl-4-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-4-hydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxypalmitorail-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3,4-dihydroxy-spinganine,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitoryl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitoryl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyvaseyl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyarakydyl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-4-hydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitriyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinolenoyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3,4-dihydroxy-4-spingenin,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitoryl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitoryl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyvaseyl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-4-hydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxypalmitorail-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3,4-dihydroxy-8-spingenin,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxypalmitorail-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3-hydroxy-4,8-spinadienine,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylignoceoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxypalmitoryl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyvaseyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-4-hydroxy-4,8-spinadienine,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylignoceoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3,4-dihydroxy-4,8-spinadienine,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxypalmitorail-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3,4-dihydroxy-4,8-spinadienine,

1- O- β-glucosyl- N- 2'-hydroxybaceoyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-3,4-dihydroxy-4,8-spingadiene and

1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3,4-dihydroxy-4,8-spingadiene.

The compounds can be used alone or in combination of two or more compounds in the plant disease resistance inducing composition according to the present invention.

Moreover, according to a preferable aspect of the present invention, the spino lipids are 4,8-spinadienine, and the following compounds are preferable examples of such 4,8-spinadienine.

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxypalmitorail-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3-hydroxy-4,8-spinadienine,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylignoceoyl-3-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl-N-2'-hydroxypalmitoryl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyvaseyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-4-hydroxy-4,8-spinadienine,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylignoceoyl-4-hydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3,4-dihydroxy-4,8-spinadienine,

1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxypalmitorail-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxystearoyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3,4-dihydroxy-4,8-spinadienine,

1- O- β-glucosyl- N- 2'-hydroxybaceoyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3,4-dihydroxy-4,8-spingadiene,

1- O- β-glucosyl- N- 2'-hydroxybehenyl-3,4-dihydroxy-4,8-spingadiene and

1- O- β-glucosyl-N-2'-hydroxylignoceoyl-3,4-dihydroxy-4,8-spingadiene.

The compounds may be used alone or in combination of two or more compounds in the plant disease resistance induction composition according to the present invention.

According to another aspect of the present invention, the amphiphilic substance is at least one selected from the group consisting of selbroside, phosphatidylcholine, sulfatide, spinomierin, phosphatidylserine and diacylglycerol. In another preferred embodiment of the invention, the amphiphilic material is a mixture comprising at least spino lipids, glycero lipids and diacylglycerols. According to another preferred embodiment of the present invention, the amphiphilic substance is a mixture containing at least cerebroside, phosphatidylcholine and diacylglycerol.

In addition, the amphiphilic substance in the present invention is preferably obtained from at least one plant selected from flow crops, grains and legumes. In addition, a plant may be used as it is as a plant raw material of an amphiphilic substance in the present invention, or plant processed products such as by-products in the food manufacturing process may be used. Thus, according to a preferred embodiment of the present invention, the plant material of the amphiphilic substance is at least one selected from the group consisting of corn, rapeseed seeds, sesame seeds, soybeans, rice, wheat, and barley. According to another preferred embodiment of the present invention, the plant material of the amphiphilic substance is at least one selected from the group consisting of cornburan, gluten wheat, rapeseed gourd, sesame gourd, soybean meal, rice bran and bran.

In addition, as a method for producing a plant disease resistance induction composition according to the present invention, when extracting an amphiphilic substance from a plant raw material, a mixed solvent of two or more substances selected from the group consisting of water, a surfactant and an organic solvent as an extraction solvent Or an organic solvent may be used alone. Therefore, according to a preferred embodiment of the present invention, the amphiphilic material is extracted from a plant raw material by a mixed solvent or an organic solvent of two or more substances selected from the group consisting of water, a surfactant and an organic solvent. In this extraction, the plant material is preferably immersed in the mixed solvent or the organic solvent and stirred.

As a mixed solvent used for extraction, the solvent which added the appropriate amount of water to the organic solvent, the solvent which contains the organic solvent and surfactant in a suitable ratio, or the solvent which consists of water and surfactant, etc. are mentioned, for example. In the case of using a surfactant in extraction, a mixed solvent containing a surfactant may be prepared first, followed by dipping and stirring the plant material in the mixed solvent, or after dipping and stirring the plant material in a solvent containing no surfactant. You may add surfactant in this solvent. According to a preferred embodiment of the present invention, the mixed solvent comprises water and a surfactant. The surfactant is added here to preferably less than 1% (w / w), more preferably 0.1 to 0.5% (w / w) relative to water.

Examples of the surfactant in the mixed solvent include nonionic (also referred to as "non-ionic") surfactants, anionic surfactants, cationic surfactants, and the like. It can mix and use. Examples of the nonionic surfactants include polyoxyethylene nonylphenyl ether, polyoxyethylene alkyl ether, polyoxyethylene sorbitan ester, polyoxyethylene phenyl ether, sorbitan fatty acid ester, polyoxyethylene fatty acid ester, and the like. Examples of the anionic surfactants include alkylbenzene sulfonates, alkyl ether sulfates, sulfonates, alkylnaphthalene sulfonates, and the like, and cationic surfactants include alkylamine salts and quaternary ammonium salts. In the present invention, it is preferable to use a surfactant used as a food additive in terms of safety, specifically, propylene glycol fatty acid ester, chondroitin sulfate, sucrose fatty acid ester, sorbitan fatty acid ester, glycerin fatty acid ester, medium chain fatty acid triglyceride, Sorbitol, mannitol, and organic acid monoglycerides such as succinic acid monoglyceride, citric acid monoglyceride, and the like.

Examples of the organic solvent used for extraction include ethanol, methanol, hexane, petroleum ether, ethyl ether, ethyl acetate, chloroform or acetone. As the solvent having good extraction efficiency, the organic solvent in the present invention is preferably ethyl acetate or chloroform, and ethanol or methanol is preferable when used as an agricultural material for cultivation of organic agricultural products.

In addition to the extraction method using an organic solvent or a surfactant as described above, a method using a supercritical fluid, a method by circulating multistage pressurization (for example, Japanese Patent No. 3212278 or 3212940). ) And the like can be used. In order to improve the yield of the extraction, an operation of re-extracting the active ingredient remaining in the residue, that is, an operation of adding the extraction solvent again to the residue obtained by separating the solid-liquid may be performed.

In addition, the extraction conditions are preferably carried out under suitable conditions depending on the properties of the plant raw material, the type of organic solvent and surfactant used, the temperature of the liquid during extraction is preferably 15 ~ 80 ℃, more preferably 25 ~ 60 ℃.

In the present invention, the plant disease resistant induction composition is preferably obtained by concentrating the extract obtained by the above extraction and precipitating and removing the physiologically harmful substances of the plant. By this concentration, the mixing ratio of the active ingredients in the composition is increased, and the physiologically harmful substances of the plant can be precipitated and removed. Here, the "physiologically inhibiting substances" of plants refers to a substance that inhibits the growth of crops by causing chlorosis on the foliage tissue when processed on crops.

By increasing the content of the amphiphilic substance as an active ingredient in the concentration process, the plant disease resistance inducible composition according to the present invention can exhibit a stronger affinity, thereby exhibiting a stronger electrodeposition action. The concentration of the active ingredient exhibiting sufficiently strong electrodeposition is preferably 0.001 to 0.4% (w / w), more preferably 0.01 to 0.1% (w / w). At such concentrations of the active ingredients, the electrodeposition properties of the electrodeposited leaves at the time of spraying and the absorption at the time of invasion treatment are improved, so that the amphiphilic substance as an active ingredient is improved in the electrodeposition properties and penetration performance of plants. There is a number. In other words, since the plant disease resistance induction composition according to the present invention exhibits an electrodeposition effect, it is not necessary to add an electrodeposition agent during application or only by adding a small amount, and in this respect, it is possible to reduce the load on the environment. Will be. Therefore, according to a preferred embodiment of the present invention, the plant disease resistance induction composition has a content of amphiphilic material of 0.001 ~ 0.4% (w / w).

Concentration may be carried out by conventional concentration methods such as freeze drying, reduced pressure concentration, heat distillation, etc., but concentration at high temperature is not preferable, so it is preferable to carry out under reduced pressure concentration. It concentrates as follows. As a concentration target, the volume ratio is preferably 1/2 to 1/40, more preferably 1/4 to 1/12, so that the volume can be reduced. It is preferable to concentrate to 0.001 to 0.4% (w / w).

The production method according to the present invention preferably further comprises isolating the plant disease resistant inducible composition from the concentrate obtained by concentration. The isolation method is not particularly limited, and a known method can be suitably used, and examples thereof include a method of filtering a concentrated liquid and the like.

The formulation of the plant disease resistant inducible composition according to the present invention may be in any form of agrochemicals such as liquids, powders, granules, emulsions, hydrating agents, emulsions, aerosols, and blowables. The method of application is not particularly limited.

According to a preferred embodiment of the present invention, a plant disease resistant induction composition is used as a plant control agent. According to another embodiment of the present invention, there is provided a method for controlling plant diseases comprising the step of treating a target plant by the plant disease resistant inducible composition according to the present invention.

Means for treating the target plant in the control method may include, but are not limited to, spraying treatment, infiltration treatment, irrigation treatment, watering treatment, and the like. In addition, by combining the above treatment means in accordance with the stage of the crop or the method of cultivating the crop, the effect and duration of the drug can be made more effective. In addition, the treatment concentration and the number of times of treatment of the disease-resistant inducing composition according to the present invention are determined by the type of crop, the degree of growth of the crop, the developmental patterns of plant diseases such as ground diseases and soil diseases, and the onset pressure of the density of pathogens. You can choose the enemy in response.

The target crops that can be treated with the plant disease resistant inducible composition of the present invention include all cultivated plants, such as cereals (rice, barley, wheat, corn, etc.), eggplants (tomatoes, eggplants, etc.), gourds and plants. (Cucumbers, melons, pumpkins, etc.), legumes (peas, soybeans, etc.), fluid plants (beet, cabbage, cabbage, etc.), and rose plants (strawberries, apples, pears, etc.). In addition, the target crop is preferably a plant separate from the plant material of the plant disease resistance induction composition according to the present invention. For example, when the target crop to be treated is rice, plant raw materials other than rice are effective for the raw material of the plant resistance inducing composition according to the present invention.

In addition, since the general disease resistance response of plants should be considered to be non-specific to pathogens, the target diseases of the crops include all plant diseases caused by filamentous fungi, such as lettuce root rot, tomato counterfeit disease and wheat red mold. Bottle, melon vine disease, strawberry udon powder, cucumber anthrax, cabbage bottle, spinach crab disease, soybean purpura, eggplant powder, green rust, and rice sticky disease. And the plant resistance inducing composition according to the present invention is effective for any of airborne ground pests and soil infectious soil pests.

The present invention is further explained with the following examples, but the present invention is not limited only to these examples.

Example  1: Disease resistance induction composition water  Produce

Various plant raw materials were pulverized with a power mill (Showa-Kien Co., Ltd., tabletop power mill P-02) as needed, and then made into a pulverized product using a screen eye size of 1.0 mm, and tested according to the following steps. .

(1) extraction

As an extraction method using an organic solvent, 6 liters of 99.5% ethanol was added to 1 kg of plant powder raw material, and extraction was performed while stirring at room temperature. After 18 hours of extraction, ethanol extract and residue were obtained by solid-liquid separation.

In addition, the extraction method using a surfactant was immersed and swelled in 12 liters of water with respect to 1 kg of plant powder raw materials, and then various surfactants were added so as to have final concentrations of 0.1%, 0.3%, 0.5%, and 1.0% according to the test contents. The extraction was carried out for 18 hours while stirring under room temperature. And the surfactant extract was obtained by removing an insoluble matter by centrifugation (2,000xg, 20 minutes).

(2) Concentration and isolation

About the ethanol extract obtained by the said extraction process, it concentrated to about 1/12 of the original volume under reduced pressure using the rotary evaporator (Asahi make, ARE-20V). After concentration, the obtained ethanol extract was cooled to room temperature, and the precipitate formed in the liquid was further removed using No. 2 filter paper (trademark; ADVANTEC, manufactured by Toyo Roshi Kaisha, Ltd.) to obtain a concentrated solution of about 400 mL.

Moreover, about the surfactant extract liquid obtained at the said extraction process, it concentrated and dried under reduced pressure using the rotary evaporator similarly to the above. 40 mL of ethanol was added and dissolved in the obtained dry solid, and the insoluble substance was removed using No. 2 filter paper to obtain a surfactant extract concentrate.

Example  2

In order to investigate the disease control effect of the disease-resistant induction composition according to the present invention was carried out a control test for rice blast. First, seedlings of rice (variety: Akidago-machi) are seeded with 8 eggs per pot, cultivated in artificial weather room, and various stages of 3-4 leaf age of main leaves. Samples Samples (2 mL per pot) were subjected to foliage spraying (wherein 4 ports per 1 sphere). In the positive control, selbroside B solution (Celebroside B concentration: 10 ppm) was similarly sprayed, and in the negative control, water was sprayed. Then, the solution was left to stand for about 1 hour until it was dried on the foliar surface, and then grown again in the growing room. On the other hand, as the cerebroside B was used a standard (標: Selebroside B concentration 2mg / mL solution) separated and purified by Umemura et al. (Plant Cell Physiology, 2000, 41, 676-683).

After 3 days of sample treatment, rice blast bacteria ( Magnaporthe Forced infection by spray inoculation of the conidia suspension of grisea race 007) was performed. After spray inoculation, infection of the bacterium was promoted by standing for 36 hours in a dark, humidified condition. Thereafter, the test plants were transferred to the artificial weather room and grown, and the control value was calculated by measuring the number of pathogenic diseases occurring in the third main leaves of each sphere after 6 days of inoculation. In addition, the control value was computed according to the following formula | equation.

[Equation 1]

Control value = (1-average number of lesions per leaf of each sphere / average number of lesions per leaf of control) x l00

Table 1 shows the control effect of the 100-fold dilution of the ethanol extract concentrate prepared by the method of Example 1 using various plant raw materials. The ethanol extract concentrates of plant materials other than rice bran were recognized for their control effect, but all showed control effect.

TABLE 1

      Control         Konburan        68         Gluten wheat        55         Fluid foil        73         Sesame        47         Rice bran         8         bran        70         Soybean meal        69         Celebbroside B        82

In addition, using a 0.3% DK ester SS (manufactured by Daiichi Kogyo Pharmaceutical Co., Ltd.) which is a surfactant (nonionic) of a food additive, various plant raw materials were added to a 50-fold dilution of the surfactant extract concentrate prepared by the method of Example 1. Table 2 shows the effects of the control. As in the case of the ethanol extract concentrate, the surfactant extract concentrate made from plants other than rice bran showed a control effect.

TABLE 2

      Control         Konburan        75         Gluten wheat        61         Fluid foil        56         Sesame        35         Rice bran         3         bran        65         Soybean meal        58         Celebbroside B        83

In order to easily compare the extraction efficiency of the active ingredient according to the type and concentration of the surfactant, the white alexin inducing ability of the rice was investigated for each surfactant extract concentrate. Rice white alexin has been reported so far with momilactone, olijalexin, sacranetin and white caric acid, but inducing pest resistance by using momi lactone and white caric acid, which are highly correlated with rice blast resistance. (Koga j, Ann. Phytopathol. Soc. Jpn. (1998) 64,97-101, Umemura K, Biosci. Biotechnol. Biochem. (2003) 67, 899-902). Specifically, DK ester SS and S-215 (nonionic surfactant of food additives, manufactured by Mitsubishi Chemical Foods Co., Ltd.) are used as surfactants, and 50-fold dilutions of the surfactant extract concentrates extracted under various concentration conditions are used. White Alexin induction was measured. The results were as shown in Table 3. Here, this result is shown by the relative value at the time of making activity 100 when processing using celecbroside B (10 ppm).

TABLE 3

 Surfactants           DK ester SS          S-215  Surfactant Concentration (%)   0   0.1  0.3  0.5  1.0   0  0.1  0.3  0.5  1.0  Konburan   2   64  185  213  198   3   51   85  121  143  Gluten wheat   4   46   86  132  135   2   35   74   70   71  Fluid foil   0   26   43   46   47   0   23   13   30   31  Sesame   0   13   38   39   38   0   43   43   43   35  Rice bran   0    2    One    3    2   0    One    One    0    One  bran   0   23   25   23   23   0   33   35   38   43  Soybean meal   2   43   46   59   63   2   43   45   49   53

When the extraction operation was carried out without adding the surfactant, almost no active ingredient showing various disease resistance induction was extracted. On the other hand, by increasing the concentration of the surfactant it was recognized that the trend of increasing the extraction efficiency of the active ingredient. However, when the concentration of the surfactant was 1.0% or more, no further improvement in the extraction efficiency was observed, and phenomena such as an increase in viscosity of the extract during the concentration of the post-process, an increase in time required for concentration, and a decrease in operability were recognized.

In addition, in order to compare the extraction efficiency of each surfactant (0.5% addition), the result at the time of extracting cornburan as a raw material is put together in Table 4. The disease resistance inducible activity value in Table 4 is a value showing white axin induction ability in 50-fold dilutions of each surfactant extract concentrate, and is a relative value when the activity of 10 ppm treatment of selbroside B is 100. .

TABLE 4

Surfactants Used in Extraction Process     Type of surfactant (sales agency) Disease resistance induction activity (relative value)   S-40   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     40   S-215   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)    121   S-220   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)    104   K-220   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     97   K-230   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     86   HC-60   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     70   P-208   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     68   HT-208   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     40   NK-60   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     56   NK-l00C   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     89   TritonX-100   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     87   DK ester SS   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)    113   LWA-1670   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     97   L-1695   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     69   M-1695   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     52   P-1670   Nonionic (Mitsubishi Chemical Foods Co., Ltd.)     44   H-20   Anionic (Ito Oil Co., Ltd.)     67   SUPERIC S R-70   Anionic (Ito Oil Co., Ltd.)     58   BIO-SOFT D40   Anion system (Tomoe Koup Co., Ltd.)     46   SUPERIC # 518   Cationic (Ito Oil Co., Ltd.)     72   AMMONYX 4 Cationic (Tomoe Industrial Co., Ltd.)     36

Example  3: lettuce Root rot  Control test

(1) method

Seedlings of lettuce (variety: Patriot) with three main leaves developed were immersed, irrigated or foliage sprayed using a 100-fold dilution of the ethanol extract concentrate prepared in Example 1. After this treatment, lettuce seedlings were transplanted into contaminated soil (Fusarium oxysporum f. Sp.lactucae race SB1-1 strain adjusted per 1g by the dilution plate method adjusted to the average density of 3 x 10 ⁴ CFU). Inoculation treatment was carried out. As a positive control, celbroside B solution (celebroside B concentration: 10 ppm) was used.

After 21 days after transplantation, the onset of the internal symptom was investigated and the control effect of each test solution was examined. Here, the incidence index is 0 when there is no outbreak, 1 when less than 1/3 of the flow pipe discolors, and 2 when 1/3 to 2/3 of the flow pipe discolors. The case where 2/3 or more discolored was made into 3. And the control value was computed by the formula shown below.

[Equation 2]

(Average onset of control-average onset of test) / (average onset of control) x l00

(2) results

The results were as shown in Table 5. The control effect was recognized even when the ethanol extract concentrate derived from any plant material was used. In addition, the control effect was recognized in any of the immersion treatment, irrigation treatment and spraying treatment, and the tendency of immersion treatment to exhibit the highest control value was recognized.

TABLE 5

 Immersion treatment  Irrigation   Spray treatment     Konburan    84    57    55     Gluten wheat    79    75    28     Fluid foil    45    34    12     Sesame    34    23    35     Rice bran    62    55    32     bran    56    45    30     Soybean meal    78    45    23    Celebbroside B    98    86    72

Example  4: tomato counterfeit ( 萎凋 Bottle Control Test

(1) method

Seeds of tomato (variety: Fonteroza), which had four main leaves, were immersed for 36 hours using 50-fold or 100-fold dilutions of the ethanol extract concentrate. Here, the ethanol extract concentrate was prepared by the method of Example 1. Thereafter, implanted in the contaminated soil for seedlings of the tomato (tomato forged germs (Fusarium oxyporum f. Sp. Lycopersici ) race J-1 Note bacteria density will adjusted to 3 x 10 ⁴ CFU / g) was the inoculation treatment . The selbroside B solution (Celebroside B concentration: 5 ppm or 10 ppm) was used as a positive control.

(2) results

Outbreak investigation similar to Example 3 was performed 24 days after transplantation, and the control effect of each test liquid was investigated. The results were as shown in Table 6. The control effect was also recognized in all plant-derived ethanol extract concentrates.

TABLE 6

 Ethanol Extract Concentrate   Dilution factor   50-fold dilution   100-fold dilution   Konburan     75     63   Gluten wheat     62     57   Fluid foil     52     49   Sesame     24     25   Rice bran     61     48   bran     46     37   Soybean meal     57     55   Celebbroside B     68 (100ppm)     61 (50 ppm)

Example  5: wheat red mold control test

(1) method

The control test was performed using wheat grown in the Meiji Seika Co., Ltd. (Kayama 800, Odawara City) farm (No. 61). One test zone was 10 m ² , and in the spraying treatment, the test solution was used at a rate of 150 L / 10a per test zone. The spraying treatment was performed three times in total for each test zone, which was a sowing machine, a water extraction machine, and a hard water period. In addition, straw was placed on the furrows to promote spontaneous development as an infection treatment, and sprinkling by sprinkler was performed to become a humid condition. A 50-fold dilution of the ethanol extract concentrate derived from plant raw materials was used as the test solution, and a 1000-fold dilution of trifumin hydrate (manufactured by Soda Co., Ltd.) was used as a positive control.

(2) results

The results were as shown in Table 7. The control value was calculated based on the incidence of the earliest red fungal disease. The control effect was recognized except the ethanol extract concentrate which used wheat as the raw material as the test plant.

TABLE 7

   Control   Konburan    49   Gluten wheat    36   Fluid foil    56   Sesame    42   Rice bran    56   bran     4   Soybean meal    48   Celebrity B    64   Torifin    78

Claims (25)

A plant disease resistant induction composition comprising an amphiphilic substance derived from a plant as an active ingredient. The plant disease resistance inducing composition according to claim 1, wherein the plant is at least one selected from the group consisting of flow crops, grains and legumes. The method of claim 1, wherein the plant material of the amphipathic material is at least one plant selected from the group consisting of corn, fluid seeds, sesame, soybeans, rice, wheat or barley. The plant disease resistance inducing composition according to claim 1, wherein the plant material of the amphiphilic substance is at least one selected from cornburan, gluten wheat, fluid foil, sesame jelly, soybean meal, rice bran and bran. The plant disease resistance inducing composition according to any one of claims 1 to 4, wherein the amphiphilic substance is simple lipid, complex lipid or a mixture thereof. 6. The plant disease resistance inducing composition according to claim 5, wherein the complex lipid is phosphorus lipid and / or sugar lipid, spino lipid, spinosaccharide lipid, selbroside, glycerosaccharide lipid, or phosphatidylcholine. The method of claim 5, wherein the composite lipid 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxypalmitriyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxyvaseyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-4-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-4-hydroxy-spinganine, 1- O- β-glucosyl-N-2'-hydroxypalmitriyl-4-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-4-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-4-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxyvaseyl-4-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-4-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-4-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-4-hydroxy-spinganine 1- O- β-glucosyl- N- 2'-hydroxybehenyl-4-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-4-hydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxypalmitorail-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3,4-dihydroxy-spinganine, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitoryl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitoryl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyvaseyl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyarakydyl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-4-hydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitriyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinolenoyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3,4-dihydroxy-4-spingenin, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitoryl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitoryl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyvaseyl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-4-hydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxypalmitorail-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3,4-dihydroxy-8-spingenin, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxypalmitorail-3-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-3-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxyvaceoyl-3-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3-hydroxy-4,8-spinadienine, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-3-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxylignoceoyl-3-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-4-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-4-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxypalmitoryl-4-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-4-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-4-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxyvaseyl-4-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-4-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-4-hydroxy-4,8-spinadienine, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-4-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-4-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxylignoceoyl-4-hydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxymyristoyl-3,4-dihydroxy-4,8-spinadienine, 1- O- β-glucosyl- N- 2'-hydroxypalmitoyl-3,4-dihydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxypalmitorail-3,4-dihydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxystearoyl-3,4-dihydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxyoleoyl-3,4-dihydroxy-4,8-spinadienine, 1- O- β-glucosyl- N- 2'-hydroxybaceoyl-3,4-dihydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxylinoreoyl-3,4-dihydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxylinorenoyl-3,4-dihydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxyarachidyl-3,4-dihydroxy-4,8-spingadiene, 1- O- β-glucosyl- N- 2'-hydroxybehenyl-3,4-dihydroxy-4,8-spingadiene and 1- O- β-glucosyl- N- 2'-hydroxylignoseroyl-3,4-dihydroxy-4,8-spingadiene, characterized in that at least one member selected from the group consisting of Plant disease resistant induction composition. The plant disease resistance inducing composition according to claim 5, wherein the simple lipid is diacylglycerol. The method according to any one of claims 1 to 4, wherein the amphiphilic substance is at least one member selected from the group consisting of celecbroside, phosphatidylcholine, sulfatide, spinomierin, phosphatidylserine and diacylglycerol. Plant disease resistant induction composition. The plant disease resistance inducing composition according to any one of claims 1 to 4, wherein the amphipathic substance is a mixture containing at least spino lipids, glycero lipids and diacylglycerols. 5. The plant disease resistance inducing composition according to claim 1, wherein the amphiphilic substance is a mixture containing at least celebroside, phosphatidylcholine and diacylglycerol. 6. The method according to any one of claims 1 to 11, characterized in that the amphiphilic material is extracted from a plant material by a mixed solvent or an organic solvent of two or more substances selected from the group consisting of water, a surfactant and an organic solvent. Plant disease resistant induction composition. 13. The plant disease resistance inducing composition according to claim 12, wherein the amphipathic substance is obtained by concentrating an extract obtained by the extraction and precipitating and removing the physiologically inhibiting substance of the plant. 13. The plant disease resistance inducing composition according to claim 12, wherein the mixed solvent comprises water and a surfactant. 13. The plant disease resistance inducing composition according to claim 12, wherein the surfactant is selected from nonionic surfactants, anionic surfactants, cationic surfactants or mixtures thereof. The method of claim 12, wherein the surfactant is selected from the group consisting of propylene glycol fatty acid esters, chondroitin sulfate, sucrose fatty acid esters, sorbitan fatty acid esters, glycerin fatty acid esters, medium chain fatty acid tricyclides, sorbitol, mannitol, and organic acid monoglycerides. Plant disease resistance induction composition, characterized in that at least one selected. 13. The plant disease resistance inducing composition according to claim 12, wherein the organic solvent is selected from methanol, ethanol or a mixture thereof. 13. The plant disease resistant inducible composition according to claim 12, wherein the extraction is performed at 15 to 80 ° C. The plant disease resistance induction composition according to claim 13, wherein the concentration is performed at 80 ° C or lower. 20. The plant disease resistant induction composition according to any one of claims 1 to 19, wherein the content of the amphipathic substance is 0.001 to 0.4% (w / w). The plant disease resistance inducing composition according to any one of claims 1 to 20, which is used as a plant control agent. A plant disease control method comprising the step of treating a target plant with the plant disease resistant inducible composition according to any one of claims 1 to 21. Amphipathic substances from plant raw materials are extracted with a mixed solvent or organic solvent of two or more substances selected from the group consisting of water, surfactants and organic solvents, The method for producing a plant disease resistance induction composition according to any one of claims 1 to 21, wherein the extract obtained by the extraction is concentrated to precipitate out the physiologically harmful substances contained in the extract. 24. The method of claim 23, further comprising isolating said composition from the concentrate obtained by said concentration. 25. The method of claim 23 or 24, wherein the content of the amphipathic substance is concentrated to 0.001 to 0.4% (w / w) based on the composition.