CN1345540A - Carposome extract used for inducing plant disease resistance - Google Patents
Carposome extract used for inducing plant disease resistance Download PDFInfo
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- CN1345540A CN1345540A CN 01130601 CN01130601A CN1345540A CN 1345540 A CN1345540 A CN 1345540A CN 01130601 CN01130601 CN 01130601 CN 01130601 A CN01130601 A CN 01130601A CN 1345540 A CN1345540 A CN 1345540A
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- fungus sporophore
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Abstract
The invention relates to a method for extracting polysaccharide form agaricus blazei and showing the effect of against vegetable disease. The preparation steps include following: crushing up materials, hot water extracting, decompression condensing, ethanol depositing and drying. the prepared product uses aqua with silicon fluid being as penetrating agent, spraying on plant of cucumber and tomato in growing period to activate defense system of plant oneself, so as to reach the goal. It is effective for following disease categories: downy mildew, anthracnose etc.
Description
Fungi Agaricus blazei Murrill (Agaricus blazei) fruiting body extract is a kind of low toxin preparation as plant disease resistance inductor, and its disease-resistant principle is to induce the also disease-resistant system of defense of activated plant self.Direct purpose of the present invention provides safety, hypotoxic disease-resistant agricultural chemicals.
Fungus sporophore is from commodity, obtain extract through following method: fungus sporophore is through 80 ℃ of dryings, mechanical crushing to 40 order, boiling water refluxing extraction 2 hours, filter and remove residue, supernatant is evaporated to total sugar concentration and is about 30% (w/v), add 95% precipitation with alcohol, filter, precipitation is dry, obtain powder, ethanol distillation reclaims.The goods that obtain with the method can directly use as plant disease resistance inductor.Active ingredient is fungi polysaccharide and oligosaccharides.Said preparation uses formulation to be the aqueous solution, will add solvent, penetrant, wetting agent and stabilizing agent etc. in case of necessity, makes the wettable powder type.
When using the wettable powder type, working concentration calculates with solids content, generally is advisable with 0.1-1g/L, and every mu of vegetables applied once 60-75L sprayed once at interval in 3-5 days, and according to the application target difference, working concentration and sprinkling number of times can suitably be adjusted.
The surfactant that uses as solvent, penetrant and wetting agent has the sulfuric ester, high-grade aliphatic ester, alkyl aryl sulfonate, epoxides of higher alcohol etc.
Fungal extract of the present invention to the oral toxicity of mouse extremely a little less than, and contrast almost indifference, experimental result is as follows:
Tried thing: being tried thing is the fungus sporophore solution of extract, and it is liquid to be light coffee color, the about 1.0g/ml of proportion, and concentration is 1mg/ml.
Animal: healthy Kunming kind small white mouse, body weight 20-23 gram is provided by the department of the Chinese Academy of Sciences of laboratory animal section of Beijing Medical University, the quality certification number: the moving word 01-3049 of doctor.
Through trial test, it is very low to be tried thing toxicity, so according to the dosage of 0.2ml/10g body weight, it is the 20g/kg body weight that poisoning dosage is set.Animal is divided into two groups at random, and 10 every group, male and female half and half are respectively control group and experimental group.After the animal fasting 8 hours, the experimental group animal is once irritated stomach with 0.2ml/10g body weight per os and is tried thing.Control animals is irritated stomach and is given distilled water.Observe toxic reaction symptom and the weight of animals variation after the administration, observed continuously 14 days.
Result of the test: animal does not see the overt toxicity reaction symptom after irritating the stomach contamination.Female contamination treated animal body weight and control group relatively decrease, and buck body weight change difference does not have significance.Do not find animal dead in observation period.Postmortem behind the sacrifice of animal, each internal organs do not see that overt toxicity changes.
Conclusion: fungus sporophore extract its mouse oral is irritated the stomach maximal tolerance dose more than the 20g/kg body weight.The per os acute toxicity belongs to low toxicity.
Embodiment: 1. downy mildew resistance: fungus sporophore extract (hereinafter to be referred as disease-resistant derivant) is made concentration 0.6mg/ml aqua and is aided with bleeding agent silicone oil, after treating that cucumber seedling grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval, in contrast with the plant of spraying clear water at every turn.In same time inoculation bacterium of downy mildew of cucumber, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 33.4-49.5%, and the disease-resistant lasting period is more than 6 days.2. anti-anthracnose: fungus sporophore extract (hereinafter to be referred as disease-resistant derivant) is made concentration to be the 0.6mg/ml aqua and to be aided with bleeding agent silicone oil, after treating that cucumber seedling grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval, in contrast with the plant of spraying clear water at every turn.In same time inoculation cucumber anthracnose, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 43.6-54.8%.3. mildew-resistance: fungus sporophore extract (hereinafter to be referred as disease-resistant derivant) is made concentration to be the 0.6mg/ml aqua and to be aided with bleeding agent silicone oil, after treating that cucumber seedling grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval, in contrast with the plant of spraying clear water at every turn.In same time inoculation powdery mildew of cucumber bacterium, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 31.8-34.6%.4. scab resistant: fungus sporophore extract (hereinafter to be referred as disease-resistant derivant) is made concentration to be the 0.6mg/ml aqua and to be aided with bleeding agent silicone oil, after treating that cucumber seedling grows a slice true leaf, spray disease-resistant derivant secondary, three times, four times, induced 2 days at interval, in contrast with the plant of spraying clear water at every turn.In same time inoculation dosporium cucumerinumand its, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 56.8-78.4%.5. anti-shot hole: fungus sporophore extract (hereinafter to be referred as disease-resistant derivant) is made concentration to be the 0.6mg/ml aqua and to be aided with bleeding agent silicone oil, after treating that tomato seedling grows five compound leaves, spray disease-resistant derivant 2 times, induced 3 days at interval, in contrast with the plant of spraying clear water.In same time inoculation Streptomyces scabies, when spraying the adjoining tree morbidity of clear water, begin the classification investigation, preventive effect increases and increases according to spraying number of times, and scope is 70.92-74.73%.Bacterial classification explanation: strain name: Agaricus blazei Murrill (Agaricus blazei); China Committee for Culture Collection of Microorganisms registers on the books at the common micro-organisms center and is numbered: CGMCC No.0567.
Claims (6)
1. the extract that the present invention relates to a kind of fungus sporophore is applied to anti-plant disease, and content comprises the preparation of fungus sporophore extract and uses the using method of this kind extract opposing plant disease.
2. the described method for preparing extract from fungus sporophore of the present invention claim 1 comprises: strain name, fungus sporophore pulverizing, hot water lixiviate, concentrating under reduced pressure, precipitation with alcohol and drying, prepared preparation is a pulvis, main active ingredient is fungi polysaccharide and oligosaccharides, and they have induces the also function of the disease-resistant system of defense of activated plant self.
3. the using method of the anti-plant disease of the described fungus sporophore extract of the present invention claim 1 comprises: with the powderous preparations of the described method acquisition of the present invention claim 2, making concentration is millesimal aqua, and auxiliary spray in vegetables with bleeding agent, the applied once amount is 60-75 liter/mu.
4. the described bleeding agent of the present invention claim 1 is a silicone oil, using dosage is ten thousand/.
5. press the fungus sporophore extract of the present invention claim 2 preparation, and use by the described method of the present invention claim 3, anti-phytopathogen kind has Pseudoperonospora cubensis, white powder germ, anthrax bacteria, S.scabies bacterium and black star germ.
6. this invention claim 2,3,4,5 integrated applications.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011306017A CN100399906C (en) | 2001-08-15 | 2001-08-15 | Carposome extract used for inducing plant disease resistance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011306017A CN100399906C (en) | 2001-08-15 | 2001-08-15 | Carposome extract used for inducing plant disease resistance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1345540A true CN1345540A (en) | 2002-04-24 |
| CN100399906C CN100399906C (en) | 2008-07-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB011306017A Expired - Fee Related CN100399906C (en) | 2001-08-15 | 2001-08-15 | Carposome extract used for inducing plant disease resistance |
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| CN (1) | CN100399906C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100340186C (en) * | 2004-12-14 | 2007-10-03 | 复旦大学 | Himematsutake water soluble polysaccharide and its preparing process and use |
| CN102657237A (en) * | 2012-04-14 | 2012-09-12 | 西北农林科技大学 | Antiviral agent containing coriolus versicolor polysaccharide and preparation method thereof |
| CN103918728A (en) * | 2014-04-22 | 2014-07-16 | 李英 | Medicament for preventing and curing potato scab |
| CN106852496A (en) * | 2015-12-09 | 2017-06-16 | 黄建钧 | The method of the triterpenes synthesis contained by induction mushroom gill fungus |
| CN109938046A (en) * | 2019-04-18 | 2019-06-28 | 云南农业大学 | Downy mildew of garpe biochemistry Collaborative Control medicament and its application |
| CN109964949A (en) * | 2019-04-12 | 2019-07-05 | 云南农业大学 | The application of pholiota adiosapose polysaccharide extract and Propamocarb in prevention and treatment downy mildew of garpe |
| WO2020128991A1 (en) * | 2018-12-21 | 2020-06-25 | Covertis | Biologically active substance, method for manufacturing same and use thereof as an agent for protecting a biological tissue |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3597566B2 (en) * | 1994-08-02 | 2004-12-08 | 株式会社中埜酢店 | Method for producing branched polysaccharide |
| JPH11263732A (en) * | 1998-03-16 | 1999-09-28 | Ichimaru Pharcos Co Ltd | Skin preparation for external use containing mushroom extracts |
| CN1299588A (en) * | 1999-12-10 | 2001-06-20 | 广东原沣生物工程有限公司 | Application of oligosaccharide in biological pesticide |
-
2001
- 2001-08-15 CN CNB011306017A patent/CN100399906C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100340186C (en) * | 2004-12-14 | 2007-10-03 | 复旦大学 | Himematsutake water soluble polysaccharide and its preparing process and use |
| CN102657237A (en) * | 2012-04-14 | 2012-09-12 | 西北农林科技大学 | Antiviral agent containing coriolus versicolor polysaccharide and preparation method thereof |
| CN102657237B (en) * | 2012-04-14 | 2013-10-09 | 西北农林科技大学 | Antiviral agent containing coriolus versicolor polysaccharide and preparation method thereof |
| CN103918728A (en) * | 2014-04-22 | 2014-07-16 | 李英 | Medicament for preventing and curing potato scab |
| CN103918728B (en) * | 2014-04-22 | 2015-09-09 | 李英 | A kind of medicament preventing and treating deep scab of potato |
| CN106852496A (en) * | 2015-12-09 | 2017-06-16 | 黄建钧 | The method of the triterpenes synthesis contained by induction mushroom gill fungus |
| WO2020128991A1 (en) * | 2018-12-21 | 2020-06-25 | Covertis | Biologically active substance, method for manufacturing same and use thereof as an agent for protecting a biological tissue |
| FR3090275A1 (en) * | 2018-12-21 | 2020-06-26 | Covertis | BIOLOGICALLY ACTIVE SUBSTANCE, MANUFACTURING METHOD THEREOF AND USE THEREOF AS PROTECTIVE AGENT FOR BIOLOGICAL TISSUE |
| CN113490419A (en) * | 2018-12-21 | 2021-10-08 | 利摩日大学 | Bioactive substances, method for producing same, and use thereof as biological tissue protective agents |
| CN113490419B (en) * | 2018-12-21 | 2023-06-23 | 利摩日大学 | Bioactive substance, method for producing same, and use of same as biological tissue protective agent |
| CN109964949A (en) * | 2019-04-12 | 2019-07-05 | 云南农业大学 | The application of pholiota adiosapose polysaccharide extract and Propamocarb in prevention and treatment downy mildew of garpe |
| CN109938046A (en) * | 2019-04-18 | 2019-06-28 | 云南农业大学 | Downy mildew of garpe biochemistry Collaborative Control medicament and its application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100399906C (en) | 2008-07-09 |
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Granted publication date: 20080709 Termination date: 20100815 |