CL2020001320A1 - Endonucleasa sexaje y esterilización en insectos. - Google Patents

Endonucleasa sexaje y esterilización en insectos.

Info

Publication number
CL2020001320A1
CL2020001320A1 CL2020001320A CL2020001320A CL2020001320A1 CL 2020001320 A1 CL2020001320 A1 CL 2020001320A1 CL 2020001320 A CL2020001320 A CL 2020001320A CL 2020001320 A CL2020001320 A CL 2020001320A CL 2020001320 A1 CL2020001320 A1 CL 2020001320A1
Authority
CL
Chile
Prior art keywords
insect
male
methods
endonuclease
nucleic acid
Prior art date
Application number
CL2020001320A
Other languages
English (en)
Inventor
Nikolay P Kandul
Omar S Akbari
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of CL2020001320A1 publication Critical patent/CL2020001320A1/es

Links

Classifications

    • A01K67/0339—
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/054—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function
    • A01K2217/058—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function due to expression of inhibitory nucleic acid, e.g. siRNA, antisense
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Los métodos de la técnica de insecto estéril guiado de precisión revelada (pgSIT) incluyen métodos para dirigir el sexado masculino en un insecto genéticamente modificado y métodos para producir una progenie de huevo de insecto macho estéril genéticamente modificado. Estos métodos incluyen la integración de por lo menos una secuencia de ácido nucleico en el genoma de un primer insecto, la cual tiene por lo menos una secuencia de ácido nucleico que tiene por lo menos un primer polinucleótido guía que se dirige a una secuencia genómica femenina esencial que se requiere para la viabilidad específica de la hembra, la introducción de una endonucleasa en un segundo insecto y el cruce genético del primer y el segundo insecto, produciendo así una progenie que expresa la endonucleasa y la secuencia de ácido nucleico por lo menos una. En el caso de la esterilidad masculina, un segundo polinucleótido guía se dirige a una secuencia genómica de esterilidad masculina que se requiere para la esterilidad específica del macho.
CL2020001320A 2017-11-21 2020-05-19 Endonucleasa sexaje y esterilización en insectos. CL2020001320A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201762589405P 2017-11-21 2017-11-21

Publications (1)

Publication Number Publication Date
CL2020001320A1 true CL2020001320A1 (es) 2021-02-12

Family

ID=66630788

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2020001320A CL2020001320A1 (es) 2017-11-21 2020-05-19 Endonucleasa sexaje y esterilización en insectos.

Country Status (11)

Country Link
US (1) US20200367479A1 (es)
EP (1) EP3713406B1 (es)
JP (1) JP2021503893A (es)
KR (2) KR102673530B1 (es)
CN (1) CN111587070A (es)
AU (1) AU2018370818A1 (es)
BR (1) BR112020010130A2 (es)
CA (1) CA3083123A1 (es)
CL (1) CL2020001320A1 (es)
IL (1) IL274580A (es)
WO (1) WO2019103982A2 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201810253D0 (en) * 2018-06-22 2018-08-08 Imperial Innovations Ltd Gene drive
WO2021016600A1 (en) * 2019-07-25 2021-01-28 The Regents Of The University Of California Methods and compositions for sexing and sterilization in drosophila suzukii and aedes aegypti
CN110452896B (zh) * 2019-08-08 2022-08-16 南京农业大学 一种植物抗虫相关蛋白OsPAL6和OsPAL8及其编码基因与应用
KR102365188B1 (ko) * 2019-11-26 2022-02-18 안동대학교 산학협력단 전자빔 조사를 이용한 호박꽃과실파리 불임 수컷의 제조방법 및 이를 이용한 호박꽃과실파리의 방제방법
US20240023528A1 (en) * 2020-05-26 2024-01-25 The Regents Of The University Of California One-locus inducible precision guided sterile insect technique or temperature-inducible precision guided sterile insect technique
EP4221498A4 (en) * 2020-09-29 2024-10-30 Univ Brandeis STERILE ORGANISMS, METHODS OF PREPARATION AND METHODS OF USE THEREOF
CN114317613B (zh) * 2020-09-30 2023-12-22 浙江省农业科学院 利用基因组编辑技术构建鳞翅目昆虫雌性不育品系的方法
CN112852806B (zh) * 2021-01-15 2022-10-14 中国农业科学院深圳农业基因组研究所 一种靶向草地贪夜蛾Doublesex基因雌性特异E3外显子的sgRNA及其应用
KR102285906B1 (ko) 2021-02-18 2021-08-04 주식회사 엔에이피 수생태계 교란어종 관리를 위한 큰입배스 불임 유도용 조성물
WO2023230572A1 (en) * 2022-05-26 2023-11-30 The Regents Of The University Of California Confinable population suppression system
CN114807144B (zh) * 2022-05-30 2023-05-12 河南大学 一种亚洲玉米螟Doublesex基因突变体及其构建方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050120397A1 (en) * 2003-11-24 2005-06-02 Hermann Steller Compounds and methods for regulation of spermatid differentiation
US10752906B2 (en) * 2013-11-05 2020-08-25 President And Fellows Of Harvard College Precise microbiota engineering at the cellular level
CN106133141B (zh) * 2014-01-08 2021-08-20 哈佛学院董事及会员团体 Rna引导的基因驱动
US20150237838A1 (en) * 2014-02-26 2015-08-27 California Institute Of Technology Regulation of insect populations
MX2016017317A (es) * 2014-06-26 2017-08-24 Regeneron Pharma Metodos y composiciones para modificaciones geneticas dirigidas y metodos de uso.
WO2016106236A1 (en) * 2014-12-23 2016-06-30 The Broad Institute Inc. Rna-targeting system
CA2970370A1 (en) * 2014-12-24 2016-06-30 Massachusetts Institute Of Technology Crispr having or associated with destabilization domains

Also Published As

Publication number Publication date
CN111587070A (zh) 2020-08-25
KR20200088446A (ko) 2020-07-22
CA3083123A1 (en) 2019-05-31
EP3713406A2 (en) 2020-09-30
WO2019103982A3 (en) 2020-03-26
IL274580A (en) 2020-06-30
KR102673530B1 (ko) 2024-06-07
JP2021503893A (ja) 2021-02-15
BR112020010130A2 (pt) 2020-11-10
KR20240096693A (ko) 2024-06-26
EP3713406B1 (en) 2024-08-21
US20200367479A1 (en) 2020-11-26
AU2018370818A1 (en) 2020-07-02
EP3713406C0 (en) 2024-08-21
WO2019103982A2 (en) 2019-05-31
EP3713406A4 (en) 2021-07-28

Similar Documents

Publication Publication Date Title
CL2020001320A1 (es) Endonucleasa sexaje y esterilización en insectos.
AR100216A1 (es) Edición de genes multiplexados
AR103927A1 (es) Método para mejorar la habilidad de resistir a adn de virus que infectan plantas
BR112016020287A2 (pt) células resistentes a vírus e usos das mesmas
MX2019006509A (es) Sistema y aparato del mismo para destruir patogenos asociados al calzado.
GB2544001A (en) Methods for increasing Cas9-mediated engineering efficiency
CL2018000750A1 (es) Método de redireccionamiento de linfocitos t para el tratamiento de la infección por vih
AR098295A1 (es) Composiciones y métodos para controlar infestaciones de plagas y parásitos de los artrópodos
CL2019001742A1 (es) Evento de élite ee-gm5 y métodos y kits para identificar dicho evento en muestras biológicas.
CL2020003057A1 (es) Plantas que contienen el evento elite ee-gm4, y métodos y kits para identificar dicho evento en muestras biológicas, y tratamiento de estas
AR103226A1 (es) Inductor de haploides
AR115975A1 (es) Animales transgénicos y embriones transgénicos productores de una nucleasa de diseño mediante ingeniería
AR108600A1 (es) Evento de élite de maíz mzhg0jg
BR112018070578A2 (pt) composições, sistemas, kits, e métodos para tratar uma infecção
CO2018008408A2 (es) Sistema para liberar ácaros beneficiosos y usos de los mismos.
CO2021002691A2 (es) Composiciones y métodos para editar el gen hidroxiácido oxidasa 1 (hao1) para tratar la hiperoxaluria primaria tipo 1 (ph1)
AR099007A1 (es) Gen restaurador rf3 de tipo s de la esterilidad masculina ciplasmática del maíz (cms), marcadores moleculares y sus usos
BR112019003049A2 (pt) sistema de fixação óssea
CL2019001739A1 (es) Evento de élite ee-gm4 y métodos y kits para identificar dicho evento en muestras biológicas.
CL2017000891A1 (es) Métodos de cultivo semicontinuo
CL2020003058A1 (es) Plantas que contienen el evento elite ee-gm5 y métodos y kits para identificar dicho evento en muestras biológicas, y tratamiento de estas
CL2017002789A1 (es) Composiciones y métodos para controlar infestasiones de plagas y parásitos de los artrópodos
CL2020000738A1 (es) Instalación de producción multi trófica desacoplada, con unidad de bio reactor.
AR103609A1 (es) Transparencia para tarjeta de medicación
CL2017003309A1 (es) Métodos de purificación y/o inactivación viral