CH670655A5 - - Google Patents
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- Publication number
- CH670655A5 CH670655A5 CH316/87A CH31687A CH670655A5 CH 670655 A5 CH670655 A5 CH 670655A5 CH 316/87 A CH316/87 A CH 316/87A CH 31687 A CH31687 A CH 31687A CH 670655 A5 CH670655 A5 CH 670655A5
- Authority
- CH
- Switzerland
- Prior art keywords
- cell
- plants
- transforming factor
- carried out
- microinjection
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 claims description 37
- 238000000034 method Methods 0.000 claims description 24
- 210000001938 protoplast Anatomy 0.000 claims description 19
- 230000001131 transforming effect Effects 0.000 claims description 15
- 238000000520 microinjection Methods 0.000 claims description 10
- 108020004414 DNA Proteins 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 230000005684 electric field Effects 0.000 claims description 4
- 230000003362 replicative effect Effects 0.000 claims description 4
- 210000003463 organelle Anatomy 0.000 claims description 3
- 239000006152 selective media Substances 0.000 claims description 3
- 102000053602 DNA Human genes 0.000 claims description 2
- 230000008929 regeneration Effects 0.000 claims description 2
- 238000011069 regeneration method Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 description 21
- 239000002609 medium Substances 0.000 description 16
- 235000015097 nutrients Nutrition 0.000 description 12
- 230000009466 transformation Effects 0.000 description 9
- 239000013612 plasmid Substances 0.000 description 8
- 244000061176 Nicotiana tabacum Species 0.000 description 7
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 7
- 206010020649 Hyperkeratosis Diseases 0.000 description 6
- 230000003115 biocidal effect Effects 0.000 description 5
- OOYGSFOGFJDDHP-KMCOLRRFSA-N kanamycin A sulfate Chemical compound OS(O)(=O)=O.O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N OOYGSFOGFJDDHP-KMCOLRRFSA-N 0.000 description 5
- 229960002064 kanamycin sulfate Drugs 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 210000003763 chloroplast Anatomy 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 3
- 239000002502 liposome Substances 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 238000010187 selection method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 description 1
- WEEMDRWIKYCTQM-UHFFFAOYSA-N 2,6-dimethoxybenzenecarbothioamide Chemical compound COC1=CC=CC(OC)=C1C(N)=S WEEMDRWIKYCTQM-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000208113 Nicotiana debneyi Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000007596 consolidation process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 229960002385 streptomycin sulfate Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3702659 | 1987-01-29 | ||
| GB8702006A GB2200367B (en) | 1987-01-29 | 1987-01-29 | Method of preparing genetically transformed cellular plant matter by micro- injection. |
| DE19873738874 DE3738874A1 (de) | 1987-01-29 | 1987-11-16 | Verfahren zur herstellung von genetisch transformierten pflanzenobjekten |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH670655A5 true CH670655A5 (enrdf_load_stackoverflow) | 1989-06-30 |
Family
ID=39357430
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH316/87A CH670655A5 (enrdf_load_stackoverflow) | 1987-01-29 | 1987-01-29 |
Country Status (3)
| Country | Link |
|---|---|
| CH (1) | CH670655A5 (enrdf_load_stackoverflow) |
| DE (1) | DE3738874A1 (enrdf_load_stackoverflow) |
| GB (1) | GB2200367B (enrdf_load_stackoverflow) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI890917A7 (fi) * | 1988-03-02 | 1989-09-03 | Schweizerische Eidgenossenschaft | Menetelmä transgeenisten kasvien valmistamiseksi |
| CA2017544A1 (en) * | 1989-05-29 | 1990-11-29 | Gunther Neuhaus | Process for the production of transgenic plants |
| US5550318A (en) * | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6803499B1 (en) | 1989-08-09 | 2004-10-12 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US7705215B1 (en) | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| ATE160174T1 (de) * | 1989-08-09 | 1997-11-15 | Dekalb Genetics Corp | Methoden und zusammensetzungen für die herstellung von stabil transformierten, fruchtbaren mais pflanzen und zellen dafür |
| US5484956A (en) * | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
| JP3209744B2 (ja) * | 1990-01-22 | 2001-09-17 | デカルブ・ジェネティクス・コーポレーション | 結実能力のある遺伝子変換コーン |
| US6946587B1 (en) | 1990-01-22 | 2005-09-20 | Dekalb Genetics Corporation | Method for preparing fertile transgenic corn plants |
| US6777589B1 (en) | 1990-01-22 | 2004-08-17 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US6329574B1 (en) | 1990-01-22 | 2001-12-11 | Dekalb Genetics Corporation | High lysine fertile transgenic corn plants |
| DE4013099A1 (de) * | 1990-04-25 | 1991-10-31 | Hoechst Ag | Verfahren zur transformation somatischer embryonen von nutzpflanzen |
| US6395966B1 (en) | 1990-08-09 | 2002-05-28 | Dekalb Genetics Corp. | Fertile transgenic maize plants containing a gene encoding the pat protein |
| ES2260886T3 (es) * | 1990-11-23 | 2006-11-01 | Bayer Bioscience N.V. | Procedimiento para transformar plantas monocotiledoneas. |
| AU2515592A (en) * | 1991-08-23 | 1993-03-16 | University Of Florida | A novel method for the production of transgenic plants |
| EP0586355A3 (en) * | 1992-08-19 | 1994-07-13 | Monsanto Co | Method for transforming monocotyledonous plants |
| US5780709A (en) * | 1993-08-25 | 1998-07-14 | Dekalb Genetics Corporation | Transgenic maize with increased mannitol content |
| US6118047A (en) | 1993-08-25 | 2000-09-12 | Dekalb Genetic Corporation | Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction |
| US6326527B1 (en) | 1993-08-25 | 2001-12-04 | Dekalb Genetics Corporation | Method for altering the nutritional content of plant seed |
| WO1999036563A1 (en) * | 1998-01-14 | 1999-07-22 | Emed Corporation | Electrically mediated cellular expression |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH666850A5 (de) * | 1983-06-30 | 1988-08-31 | Inst Biokhim I Fiziol Mikroorg | Verfahren zur durchfuehrung von mikrooperationen an zellen und einrichtung zu dessen verwirklichung. |
| CA1280081C (en) * | 1984-09-24 | 1991-02-12 | Calgene, Inc. | Plant cell microinjection technique |
-
1987
- 1987-01-29 GB GB8702006A patent/GB2200367B/en not_active Expired - Fee Related
- 1987-01-29 CH CH316/87A patent/CH670655A5/de not_active IP Right Cessation
- 1987-11-16 DE DE19873738874 patent/DE3738874A1/de active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| GB2200367A (en) | 1988-08-03 |
| DE3738874C2 (enrdf_load_stackoverflow) | 1991-06-27 |
| DE3738874A1 (de) | 1988-11-17 |
| GB2200367B (en) | 1990-08-08 |
| GB8702006D0 (en) | 1987-03-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PL | Patent ceased | ||
| PL | Patent ceased |