CH669670A5 - - Google Patents
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- Publication number
- CH669670A5 CH669670A5 CH3760/84A CH376084A CH669670A5 CH 669670 A5 CH669670 A5 CH 669670A5 CH 3760/84 A CH3760/84 A CH 3760/84A CH 376084 A CH376084 A CH 376084A CH 669670 A5 CH669670 A5 CH 669670A5
- Authority
- CH
- Switzerland
- Prior art keywords
- cells
- cell
- living
- genetic material
- embryos
- Prior art date
Links
- 210000004027 cell Anatomy 0.000 claims description 70
- 238000000034 method Methods 0.000 claims description 67
- 210000002257 embryonic structure Anatomy 0.000 claims description 64
- 239000003086 colorant Substances 0.000 claims description 43
- 108090000623 proteins and genes Proteins 0.000 claims description 34
- 102000004169 proteins and genes Human genes 0.000 claims description 25
- 235000013601 eggs Nutrition 0.000 claims description 22
- 238000010186 staining Methods 0.000 claims description 20
- 241000283690 Bos taurus Species 0.000 claims description 15
- 239000000975 dye Substances 0.000 claims description 12
- 210000004962 mammalian cell Anatomy 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 11
- 239000007850 fluorescent dye Substances 0.000 claims description 10
- 210000004602 germ cell Anatomy 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 7
- INAAIJLSXJJHOZ-UHFFFAOYSA-N pibenzimol Chemical class C1CN(C)CCN1C1=CC=C(N=C(N2)C=3C=C4NC(=NC4=CC=3)C=3C=CC(O)=CC=3)C2=C1 INAAIJLSXJJHOZ-UHFFFAOYSA-N 0.000 claims description 6
- 230000019100 sperm motility Effects 0.000 claims description 4
- 241000282887 Suidae Species 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 2
- 241000124008 Mammalia Species 0.000 claims 3
- 239000000725 suspension Substances 0.000 claims 1
- 239000002609 medium Substances 0.000 description 31
- 210000004940 nucleus Anatomy 0.000 description 30
- 239000000243 solution Substances 0.000 description 26
- 239000011550 stock solution Substances 0.000 description 24
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 22
- 210000000287 oocyte Anatomy 0.000 description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 14
- 108020004414 DNA Proteins 0.000 description 13
- 210000001161 mammalian embryo Anatomy 0.000 description 13
- 210000004508 polar body Anatomy 0.000 description 13
- 210000003855 cell nucleus Anatomy 0.000 description 11
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 10
- 241000699666 Mus <mouse, genus> Species 0.000 description 10
- 229940098773 bovine serum albumin Drugs 0.000 description 10
- 229910001868 water Inorganic materials 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 238000011161 development Methods 0.000 description 7
- 230000018109 developmental process Effects 0.000 description 7
- 238000011534 incubation Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 7
- 238000011160 research Methods 0.000 description 7
- 239000011780 sodium chloride Substances 0.000 description 7
- 238000012800 visualization Methods 0.000 description 7
- 210000000805 cytoplasm Anatomy 0.000 description 6
- 230000004720 fertilization Effects 0.000 description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 108010077544 Chromatin Proteins 0.000 description 5
- 241000282898 Sus scrofa Species 0.000 description 5
- 210000003483 chromatin Anatomy 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- 210000002308 embryonic cell Anatomy 0.000 description 5
- 238000000386 microscopy Methods 0.000 description 5
- 210000003101 oviduct Anatomy 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 4
- 210000001109 blastomere Anatomy 0.000 description 4
- 238000005352 clarification Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 210000000472 morula Anatomy 0.000 description 4
- 239000011824 nuclear material Substances 0.000 description 4
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 4
- 229940076788 pyruvate Drugs 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 241000894007 species Species 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- 230000035899 viability Effects 0.000 description 4
- 238000010867 Hoechst staining Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000011162 core material Substances 0.000 description 3
- 230000001086 cytosolic effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 229960000448 lactic acid Drugs 0.000 description 3
- 230000035800 maturation Effects 0.000 description 3
- 230000031864 metaphase Effects 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 210000000582 semen Anatomy 0.000 description 3
- 229910000162 sodium phosphate Inorganic materials 0.000 description 3
- 238000011146 sterile filtration Methods 0.000 description 3
- 239000006188 syrup Substances 0.000 description 3
- 235000020357 syrup Nutrition 0.000 description 3
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 229930182566 Gentamicin Natural products 0.000 description 2
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
- 108010003272 Hyaluronate lyase Proteins 0.000 description 2
- 102000001974 Hyaluronidases Human genes 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 2
- 239000004218 Orcein Substances 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 2
- 241000283984 Rodentia Species 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 238000007630 basic procedure Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 244000309464 bull Species 0.000 description 2
- 239000001110 calcium chloride Substances 0.000 description 2
- 235000011148 calcium chloride Nutrition 0.000 description 2
- 229910001628 calcium chloride Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 210000001771 cumulus cell Anatomy 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 239000012362 glacial acetic acid Substances 0.000 description 2
- 229960002773 hyaluronidase Drugs 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 229940001447 lactate Drugs 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
- -1 4-ethoxyphenyl Chemical group 0.000 description 1
- QFIIYGZAUXVPSZ-UHFFFAOYSA-N 8-(2,4-dihydroxy-6-methylanilino)-2-(2,4-dihydroxy-6-methylphenyl)imino-7-hydroxy-1,9-dimethyldibenzofuran-3-one Chemical compound CC1=CC(=CC(=C1NC2=C(C3=C(C=C2O)OC4=CC(=O)C(=NC5=C(C=C(C=C5C)O)O)C(=C43)C)C)O)O QFIIYGZAUXVPSZ-UHFFFAOYSA-N 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000004904 UV filter Substances 0.000 description 1
- PQLVXDKIJBQVDF-UHFFFAOYSA-N acetic acid;hydrate Chemical compound O.CC(O)=O PQLVXDKIJBQVDF-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001556 benzimidazoles Chemical class 0.000 description 1
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 210000003679 cervix uteri Anatomy 0.000 description 1
- SMNPLAKEGAEPJD-UHFFFAOYSA-N chembl34922 Chemical compound Cl.Cl.Cl.C1CN(C)CCN1C1=CC=C(NC(=N2)C=3C=C4N=C(NC4=CC=3)C=3C=CC(O)=CC=3)C2=C1 SMNPLAKEGAEPJD-UHFFFAOYSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 210000004670 early embryonic cell Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 210000000918 epididymis Anatomy 0.000 description 1
- 201000010063 epididymitis Diseases 0.000 description 1
- 230000012173 estrus Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 231100000722 genetic damage Toxicity 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000012248 genetic selection Methods 0.000 description 1
- 210000003783 haploid cell Anatomy 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 230000000394 mitotic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 235000019248 orcein Nutrition 0.000 description 1
- 230000016087 ovulation Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- 229940054269 sodium pyruvate Drugs 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US52062983A | 1983-08-05 | 1983-08-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH669670A5 true CH669670A5 (https=) | 1989-03-31 |
Family
ID=24073420
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH3760/84A CH669670A5 (https=) | 1983-08-05 | 1984-08-03 |
Country Status (10)
| Country | Link |
|---|---|
| JP (2) | JPH07104341B2 (https=) |
| AU (1) | AU581409B2 (https=) |
| CA (1) | CA1271716A (https=) |
| CH (1) | CH669670A5 (https=) |
| DE (1) | DE3427553C2 (https=) |
| FR (1) | FR2550218B1 (https=) |
| GB (1) | GB2144542B (https=) |
| MX (1) | MX161889A (https=) |
| NL (1) | NL8401137A (https=) |
| NZ (1) | NZ207393A (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10305768A1 (de) * | 2003-02-11 | 2004-08-19 | Justus-Liebig-Universität Giessen | Verfahren zum Färben von lipophilen Zellbestandteilen einer biologischen Probe |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3636991A1 (de) * | 1986-03-03 | 1987-09-24 | Transgene Gmbh | Verfahren zur uebertragung organischer und/oder anorganischer substanzen auf ei- und/oder somazellen von tieren sowie entsprechende zusammensetzungen |
| GB2214518A (en) * | 1988-01-28 | 1989-09-06 | Innofinance Altalanos Innovaci | Process and equipment for the rapid determination of the spermium cell count and/or living spermium count |
| NZ239893A (en) * | 1990-09-25 | 1993-11-25 | Hoechst Japan | A method for introducing a foreign dna into a cell |
| EP2264427B1 (en) | 1997-01-31 | 2017-05-03 | Xy, Llc | Optical apparatus with focussing reflector for converging radiation onto a flow of particles, and related method of analysis |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| DE19829495A1 (de) * | 1998-07-02 | 2000-01-05 | Jacques Paysan | Reagenzien und deren Anwendung zur Untersuchung von Wechselwirkungen zwischen zellulären Molekülen und deren Lokalisation in Zellen |
| WO2001032630A1 (fr) * | 1999-11-05 | 2001-05-10 | Aventis Pharma S.A. | Derives d'oligobenzimidazoles et leur utilisation comme agents de transfection d'adn |
| FR2800736B1 (fr) * | 1999-11-05 | 2002-08-23 | Aventis Pharma Sa | Derives d'oligobenzimidazoles, les compositions les contenant et leurs utilisations |
| US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| WO2002043486A1 (en) | 2000-11-29 | 2002-06-06 | Xy, Inc. | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
| JP4595067B2 (ja) | 2002-08-01 | 2010-12-08 | エックスワイ，エルエルシー | 低圧精子細胞分離システム |
| US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
| CA2534394C (en) | 2002-08-15 | 2013-01-08 | Xy, Inc. | High resolution flow cytometer |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| BRPI0408857B1 (pt) | 2003-03-28 | 2018-09-11 | Inguran Llc | aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo |
| ES2541121T3 (es) | 2003-05-15 | 2015-07-16 | Xy, Llc | Clasificación eficiente de células haploides por sistemas de citometría de flujo |
| DK2801363T3 (en) | 2004-03-29 | 2018-05-28 | Inguran Llc | PROCEDURE FOR STORING SORTED SPERMATOZOES |
| CA2574499C (en) | 2004-07-22 | 2016-11-29 | Monsanto Technology Llc | Process for enriching a population of sperm cells |
| CN102944456A (zh) * | 2012-11-07 | 2013-02-27 | 西北农林科技大学 | 一种观察早期胚胎体内发育时空分布的组织切片的制备方法及应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4094745A (en) * | 1973-06-22 | 1978-06-13 | John Scholefield | Method of staining microscopic organisms |
| US3872312A (en) * | 1973-07-02 | 1975-03-18 | Block Engineering | Method and apparatus for detecting and classifying nucleic acid particles |
| JPS5262080A (en) * | 1975-11-15 | 1977-05-23 | Particle Tech Inc | Method of and apparatus for classifying organism cell |
| DE2651060C3 (de) * | 1976-11-09 | 1980-06-26 | Boehringer Mannheim Gmbh, 6800 Mannheim | Differential-diagnostische Spermauntersuchung |
| CA1155041A (en) * | 1980-04-21 | 1983-10-11 | Michael E. Jolley | Fluorescent nucleic acid stains |
| CA1197186A (en) * | 1982-03-19 | 1985-11-26 | Robert A. Hoffman | Method of enumerating serologically selected cell populations |
-
1984
- 1984-03-06 NZ NZ207393A patent/NZ207393A/en unknown
- 1984-04-10 NL NL8401137A patent/NL8401137A/nl not_active Application Discontinuation
- 1984-07-13 CA CA000458836A patent/CA1271716A/en not_active Expired
- 1984-07-23 JP JP59151469A patent/JPH07104341B2/ja not_active Expired - Lifetime
- 1984-07-25 AU AU31159/84A patent/AU581409B2/en not_active Ceased
- 1984-07-26 DE DE3427553A patent/DE3427553C2/de not_active Expired - Fee Related
- 1984-07-27 GB GB08419143A patent/GB2144542B/en not_active Expired
- 1984-08-02 MX MX202236A patent/MX161889A/es unknown
- 1984-08-03 CH CH3760/84A patent/CH669670A5/de not_active IP Right Cessation
- 1984-08-03 FR FR8412355A patent/FR2550218B1/fr not_active Expired
-
1994
- 1994-02-28 JP JP6052567A patent/JPH0751059A/ja active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10305768A1 (de) * | 2003-02-11 | 2004-08-19 | Justus-Liebig-Universität Giessen | Verfahren zum Färben von lipophilen Zellbestandteilen einer biologischen Probe |
Also Published As
| Publication number | Publication date |
|---|---|
| GB8419143D0 (en) | 1984-08-30 |
| NZ207393A (en) | 1987-03-31 |
| JPH0751059A (ja) | 1995-02-28 |
| FR2550218A1 (fr) | 1985-02-08 |
| GB2144542A (en) | 1985-03-06 |
| GB2144542B (en) | 1987-05-07 |
| JPH07104341B2 (ja) | 1995-11-13 |
| DE3427553C2 (de) | 1994-04-14 |
| AU3115984A (en) | 1985-02-07 |
| MX161889A (es) | 1991-02-26 |
| FR2550218B1 (fr) | 1989-01-20 |
| JPS6044869A (ja) | 1985-03-11 |
| NL8401137A (nl) | 1985-03-01 |
| DE3427553A1 (de) | 1985-02-14 |
| AU581409B2 (en) | 1989-02-23 |
| CA1271716A (en) | 1990-07-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUE | Assignment |
Owner name: W. R. GRACE & CO.-CONN. |
|
| PL | Patent ceased |