CA3206327A1 - Method for detecting copy number of specific nucleic acid per single cell - Google Patents
Method for detecting copy number of specific nucleic acid per single cellInfo
- Publication number
- CA3206327A1 CA3206327A1 CA3206327A CA3206327A CA3206327A1 CA 3206327 A1 CA3206327 A1 CA 3206327A1 CA 3206327 A CA3206327 A CA 3206327A CA 3206327 A CA3206327 A CA 3206327A CA 3206327 A1 CA3206327 A1 CA 3206327A1
- Authority
- CA
- Canada
- Prior art keywords
- chromosome
- cell
- cells
- pcr
- droplet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020216149 | 2020-12-25 | ||
| JP2020-216149 | 2020-12-25 | ||
| PCT/JP2021/047619 WO2022138736A1 (ja) | 2020-12-25 | 2021-12-22 | シングルセルごとの特定の核酸のコピー数の検出方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3206327A1 true CA3206327A1 (en) | 2022-06-30 |
Family
ID=82157024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3206327A Pending CA3206327A1 (en) | 2020-12-25 | 2021-12-22 | Method for detecting copy number of specific nucleic acid per single cell |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240043929A1 (https=) |
| EP (1) | EP4269614A4 (https=) |
| JP (1) | JP7649442B2 (https=) |
| KR (1) | KR20230107662A (https=) |
| CN (1) | CN116802314A (https=) |
| CA (1) | CA3206327A1 (https=) |
| TW (1) | TWI906439B (https=) |
| WO (1) | WO2022138736A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116356005B (zh) * | 2023-04-28 | 2023-09-26 | 宁波熙宁检测技术有限公司 | 一种检测car-t细胞拷贝数的组合物及其用途 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8574842B2 (en) * | 2009-12-22 | 2013-11-05 | The Board Of Trustees Of The Leland Stanford Junior University | Direct molecular diagnosis of fetal aneuploidy |
| US20130130930A1 (en) | 2011-11-17 | 2013-05-23 | Bhairavi Parikh | Methods and devices for obtaining and analyzing cells |
| SG11201404899VA (en) * | 2012-02-14 | 2014-10-30 | Univ Cornell | Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions |
| AU2013302867A1 (en) * | 2012-08-13 | 2015-02-26 | The Regents Of The University Of California | Methods and systems for detecting biological components |
| JP7336091B2 (ja) * | 2016-08-02 | 2023-08-31 | 一般社団法人生命科学教育研究所 | 簡便な遺伝子検査法およびコピー数計測法ならびにその支援技術 |
| JP6234542B1 (ja) | 2016-12-27 | 2017-11-22 | 株式会社 TL Genomics | 胎児細胞由来染色体ｄｎａの取得方法 |
| KR20180102242A (ko) | 2017-03-06 | 2018-09-17 | 주식회사 우리은행 | 금융 자동화 기기의 비대면 채널 사용자 인증 방법 및 시스템 |
| US11254975B2 (en) * | 2018-05-24 | 2022-02-22 | National Center For Child Health And Development | Method of amplifying a polynucleotide of interest |
| JP2020000011A (ja) * | 2018-06-25 | 2020-01-09 | 株式会社理研ジェネシス | ゲノムｄｎａ中の標的領域のコピー数検出方法及び試薬キット |
| WO2021200749A1 (ja) * | 2020-04-01 | 2021-10-07 | 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ | 細胞の染色体又はその部分のコピー数を検出する方法 |
-
2021
- 2021-12-22 US US18/258,836 patent/US20240043929A1/en active Pending
- 2021-12-22 KR KR1020237020216A patent/KR20230107662A/ko active Pending
- 2021-12-22 WO PCT/JP2021/047619 patent/WO2022138736A1/ja not_active Ceased
- 2021-12-22 EP EP21910876.8A patent/EP4269614A4/en active Pending
- 2021-12-22 CN CN202180086085.8A patent/CN116802314A/zh active Pending
- 2021-12-22 JP JP2022571563A patent/JP7649442B2/ja active Active
- 2021-12-22 CA CA3206327A patent/CA3206327A1/en active Pending
- 2021-12-23 TW TW110148433A patent/TWI906439B/zh active
Also Published As
| Publication number | Publication date |
|---|---|
| KR20230107662A (ko) | 2023-07-17 |
| JPWO2022138736A1 (https=) | 2022-06-30 |
| TWI906439B (zh) | 2025-12-01 |
| EP4269614A4 (en) | 2025-06-18 |
| JP7649442B2 (ja) | 2025-03-21 |
| WO2022138736A1 (ja) | 2022-06-30 |
| US20240043929A1 (en) | 2024-02-08 |
| EP4269614A1 (en) | 2023-11-01 |
| TW202239967A (zh) | 2022-10-16 |
| CN116802314A (zh) | 2023-09-22 |
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Legal Events
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