KR20230107662A - 싱글 셀마다의 특정 핵산의 카피수의 검출 방법 - Google Patents

싱글 셀마다의 특정 핵산의 카피수의 검출 방법 Download PDF

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KR20230107662A
KR20230107662A KR1020237020216A KR20237020216A KR20230107662A KR 20230107662 A KR20230107662 A KR 20230107662A KR 1020237020216 A KR1020237020216 A KR 1020237020216A KR 20237020216 A KR20237020216 A KR 20237020216A KR 20230107662 A KR20230107662 A KR 20230107662A
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chromosome
cells
deletion
cell
single cell
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게이타 다카하시
도모히로 구보
준이치 츠치야
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티엘 제노믹스 인크.
티엘 제노믹스 인크.
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Wood Science & Technology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020237020216A 2020-12-25 2021-12-22 싱글 셀마다의 특정 핵산의 카피수의 검출 방법 Pending KR20230107662A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020216149 2020-12-25
JPJP-P-2020-216149 2020-12-25
PCT/JP2021/047619 WO2022138736A1 (ja) 2020-12-25 2021-12-22 シングルセルごとの特定の核酸のコピー数の検出方法

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KR20230107662A true KR20230107662A (ko) 2023-07-17

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US (1) US20240043929A1 (https=)
EP (1) EP4269614A4 (https=)
JP (1) JP7649442B2 (https=)
KR (1) KR20230107662A (https=)
CN (1) CN116802314A (https=)
CA (1) CA3206327A1 (https=)
TW (1) TWI906439B (https=)
WO (1) WO2022138736A1 (https=)

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Publication number Priority date Publication date Assignee Title
CN116356005B (zh) * 2023-04-28 2023-09-26 宁波熙宁检测技术有限公司 一种检测car-t细胞拷贝数的组合物及其用途

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014533509A (ja) 2011-11-17 2014-12-15 セルスケープ・コーポレーション 細胞を取得し、解析するための方法、装置及びキット
KR20180102242A (ko) 2017-03-06 2018-09-17 주식회사 우리은행 금융 자동화 기기의 비대면 채널 사용자 인증 방법 및 시스템

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8574842B2 (en) * 2009-12-22 2013-11-05 The Board Of Trustees Of The Leland Stanford Junior University Direct molecular diagnosis of fetal aneuploidy
SG11201404899VA (en) * 2012-02-14 2014-10-30 Univ Cornell Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
AU2013302867A1 (en) * 2012-08-13 2015-02-26 The Regents Of The University Of California Methods and systems for detecting biological components
JP7336091B2 (ja) * 2016-08-02 2023-08-31 一般社団法人生命科学教育研究所 簡便な遺伝子検査法およびコピー数計測法ならびにその支援技術
JP6234542B1 (ja) 2016-12-27 2017-11-22 株式会社 TL Genomics 胎児細胞由来染色体ｄｎａの取得方法
US11254975B2 (en) * 2018-05-24 2022-02-22 National Center For Child Health And Development Method of amplifying a polynucleotide of interest
JP2020000011A (ja) * 2018-06-25 2020-01-09 株式会社理研ジェネシス ゲノムｄｎａ中の標的領域のコピー数検出方法及び試薬キット
WO2021200749A1 (ja) * 2020-04-01 2021-10-07 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ 細胞の染色体又はその部分のコピー数を検出する方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014533509A (ja) 2011-11-17 2014-12-15 セルスケープ・コーポレーション 細胞を取得し、解析するための方法、装置及びキット
KR20180102242A (ko) 2017-03-06 2018-09-17 주식회사 우리은행 금융 자동화 기기의 비대면 채널 사용자 인증 방법 및 시스템

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
(비특허 문헌 1) Laura Miotke, et al., "High Sensitivity Detection and Quantitation of DNA Copy Number and Single Nucleotide Variants with Single Color Droplet Digital PCR", Analytical chemistry, 86, 5, 2618-2624.
(비특허 문헌 2) Dennis J. Eastburn, et al., "Identification and genetic analysis of cancer cells with PCR-activated cell sorting", Nucleic Acids Research, 42, 16, e128.
(비특허 문헌 3) Robert W. Yucha, et al., "High-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay", EBioMedicine, 20, 217-229.
(비특허 문헌 4) Yuka Igarashi, et al., "Single Cell-Based Vector Tracing in Patients with ADA-SCID Treated with Stem Cell Gene Therapy", Molecular Therapy, Methods & Clinical Development, 6, 8-16.
(비특허 문헌 5) Samuel C. Kim, et al., "Single-Cell RT-PCR in Microfluidic Droplets with Integrated Chemical Lysis", Analytical Chemistry, 90, 2, 1273-1279.
(비특허 문헌 6) Taisuke Sato, et al., "Direct Assessment of Single-Cell DNA Using Crudely Purified Live Cells: A Proof of Concept for Noninvasive Prenatal Definitive Diagnosis", The Journal of Molecular Diagnostics, 22, 2, 2, 132-140.
(비특허 문헌 7) Unoh Ki, et al., "A Novel Bioprinting Application for the Production of Reference Material Containing a Defined Copy Number of Target DNA", Ricoh Technical Report, 2020, 44, 14-26. Retrieved from <https://jp.ricoh.com/technology/techreport/44/>
(비특허 문헌 8) Ichiro Hanamura, et al., "Frequent gain of chromosome band 1q21 in plasma-cell dyscrasias detected by fluorescence in situ hybridization: incidence increases from MGUS to relapsed myeloma and is related to prognosis and disease progression following tandem stem-cell transplantation", Blood, 108, 5, 1724-1732.
(비특허 문헌 9) 이시다 다다오, "다발성 골수종: 염색체 이상과 임상병형·예후", [online], 2013-09-21, 임상 혈액, 54, 10, 2, 1856-1866. Retrieved from <https://doi.org/10.11406/rinketsu.54.1856>

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JPWO2022138736A1 (https=) 2022-06-30
TWI906439B (zh) 2025-12-01
EP4269614A4 (en) 2025-06-18
JP7649442B2 (ja) 2025-03-21
WO2022138736A1 (ja) 2022-06-30
US20240043929A1 (en) 2024-02-08
CA3206327A1 (en) 2022-06-30
EP4269614A1 (en) 2023-11-01
TW202239967A (zh) 2022-10-16
CN116802314A (zh) 2023-09-22

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