CN116802314A - 每个单细胞的特定核酸的拷贝数的检测方法 - Google Patents

每个单细胞的特定核酸的拷贝数的检测方法 Download PDF

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Publication number
CN116802314A
CN116802314A CN202180086085.8A CN202180086085A CN116802314A CN 116802314 A CN116802314 A CN 116802314A CN 202180086085 A CN202180086085 A CN 202180086085A CN 116802314 A CN116802314 A CN 116802314A
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chromosome
cell
pcr
deletion
cells
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Pending
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CN202180086085.8A
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Chinese (zh)
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高桥启太
久保知大
土屋纯一
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TL Genomics Inc
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TL Genomics Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202180086085.8A 2020-12-25 2021-12-22 每个单细胞的特定核酸的拷贝数的检测方法 Pending CN116802314A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020216149 2020-12-25
JP2020-216149 2020-12-25
PCT/JP2021/047619 WO2022138736A1 (ja) 2020-12-25 2021-12-22 シングルセルごとの特定の核酸のコピー数の検出方法

Publications (1)

Publication Number Publication Date
CN116802314A true CN116802314A (zh) 2023-09-22

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CN202180086085.8A Pending CN116802314A (zh) 2020-12-25 2021-12-22 每个单细胞的特定核酸的拷贝数的检测方法

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Country Link
US (1) US20240043929A1 (https=)
EP (1) EP4269614A4 (https=)
JP (1) JP7649442B2 (https=)
KR (1) KR20230107662A (https=)
CN (1) CN116802314A (https=)
CA (1) CA3206327A1 (https=)
TW (1) TWI906439B (https=)
WO (1) WO2022138736A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116356005B (zh) * 2023-04-28 2023-09-26 宁波熙宁检测技术有限公司 一种检测car-t细胞拷贝数的组合物及其用途

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8574842B2 (en) * 2009-12-22 2013-11-05 The Board Of Trustees Of The Leland Stanford Junior University Direct molecular diagnosis of fetal aneuploidy
US20130130930A1 (en) 2011-11-17 2013-05-23 Bhairavi Parikh Methods and devices for obtaining and analyzing cells
SG11201404899VA (en) * 2012-02-14 2014-10-30 Univ Cornell Method for relative quantification of nucleic acid sequence, expression, or copy changes, using combined nuclease, ligation, and polymerase reactions
AU2013302867A1 (en) * 2012-08-13 2015-02-26 The Regents Of The University Of California Methods and systems for detecting biological components
JP7336091B2 (ja) * 2016-08-02 2023-08-31 一般社団法人生命科学教育研究所 簡便な遺伝子検査法およびコピー数計測法ならびにその支援技術
JP6234542B1 (ja) 2016-12-27 2017-11-22 株式会社 TL Genomics 胎児細胞由来染色体ｄｎａの取得方法
KR20180102242A (ko) 2017-03-06 2018-09-17 주식회사 우리은행 금융 자동화 기기의 비대면 채널 사용자 인증 방법 및 시스템
US11254975B2 (en) * 2018-05-24 2022-02-22 National Center For Child Health And Development Method of amplifying a polynucleotide of interest
JP2020000011A (ja) * 2018-06-25 2020-01-09 株式会社理研ジェネシス ゲノムｄｎａ中の標的領域のコピー数検出方法及び試薬キット
WO2021200749A1 (ja) * 2020-04-01 2021-10-07 株式会社 ＴＬ Ｇｅｎｏｍｉｃｓ 細胞の染色体又はその部分のコピー数を検出する方法

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Publication number Publication date
KR20230107662A (ko) 2023-07-17
JPWO2022138736A1 (https=) 2022-06-30
TWI906439B (zh) 2025-12-01
EP4269614A4 (en) 2025-06-18
JP7649442B2 (ja) 2025-03-21
WO2022138736A1 (ja) 2022-06-30
US20240043929A1 (en) 2024-02-08
CA3206327A1 (en) 2022-06-30
EP4269614A1 (en) 2023-11-01
TW202239967A (zh) 2022-10-16

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