CA3055900A1 - Codon optimization and ribosome profiling for increasing transgene expression in chloroplasts of higher plants - Google Patents

Codon optimization and ribosome profiling for increasing transgene expression in chloroplasts of higher plants

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Publication number
CA3055900A1
CA3055900A1 CA3055900A CA3055900A CA3055900A1 CA 3055900 A1 CA3055900 A1 CA 3055900A1 CA 3055900 A CA3055900 A CA 3055900A CA 3055900 A CA3055900 A CA 3055900A CA 3055900 A1 CA3055900 A1 CA 3055900A1
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Canada
Prior art keywords
codon
protein
plant
optimized
ctb
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3055900A
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English (en)
French (fr)
Inventor
Henry Daniell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pennsylvania Penn
Original Assignee
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pennsylvania Penn filed Critical University of Pennsylvania Penn
Publication of CA3055900A1 publication Critical patent/CA3055900A1/en
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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CA3055900A 2016-03-20 2017-03-20 Codon optimization and ribosome profiling for increasing transgene expression in chloroplasts of higher plants Pending CA3055900A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662310788P 2016-03-20 2016-03-20
US62/310,788 2016-03-20
PCT/US2017/023263 WO2017165320A1 (en) 2016-03-20 2017-03-20 Codon optimization and ribosome profiling for increasing transgene expression in chloroplasts of higher plants

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CA3055900A1 true CA3055900A1 (en) 2017-09-28

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CA3055900A Pending CA3055900A1 (en) 2016-03-20 2017-03-20 Codon optimization and ribosome profiling for increasing transgene expression in chloroplasts of higher plants

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US (1) US11332754B2 (enExample)
EP (1) EP3432902B8 (enExample)
JP (1) JP7145083B2 (enExample)
CN (1) CN110603046B (enExample)
CA (1) CA3055900A1 (enExample)
WO (1) WO2017165320A1 (enExample)

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WO2021003952A1 (zh) * 2019-07-08 2021-01-14 清华大学 极小量细胞核糖体保护的rna片段测序的方法

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AU2017264967B2 (en) * 2016-05-12 2024-07-25 The Trustees Of The University Of Pennsylvania Compositions and methods for inhibiting biofilm deposition and production
CN112912505A (zh) * 2018-08-22 2021-06-04 宾夕法尼亚大学托管会 在莴苣叶绿体中生产无抗生素生物药物的组合物和方法
CN111635935A (zh) * 2020-06-10 2020-09-08 华中农业大学 一种低成本、高通量间接量化蛋白表达水平的方法及其应用
EP4625419A1 (en) * 2022-11-24 2025-10-01 Nanjing GenScript Biotech Co., Ltd. Codon optimization
CN117511978B (zh) * 2024-01-08 2024-03-22 深圳粒影生物科技有限公司 一种提高外源蛋白表达量的方法

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JP7145083B2 (ja) 2022-09-30
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US20190194679A1 (en) 2019-06-27
EP3432902A1 (en) 2019-01-30
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