CA3020165A1 - Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor - Google Patents
Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor Download PDFInfo
- Publication number
- CA3020165A1 CA3020165A1 CA3020165A CA3020165A CA3020165A1 CA 3020165 A1 CA3020165 A1 CA 3020165A1 CA 3020165 A CA3020165 A CA 3020165A CA 3020165 A CA3020165 A CA 3020165A CA 3020165 A1 CA3020165 A1 CA 3020165A1
- Authority
- CA
- Canada
- Prior art keywords
- sequence
- primer
- wild
- mutant
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662319332P | 2016-04-07 | 2016-04-07 | |
| US62/319,332 | 2016-04-07 | ||
| PCT/US2017/026088 WO2017176852A1 (en) | 2016-04-07 | 2017-04-05 | Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3020165A1 true CA3020165A1 (en) | 2017-10-12 |
Family
ID=60000654
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3020165A Pending CA3020165A1 (en) | 2016-04-07 | 2017-04-05 | Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US11542547B2 (enExample) |
| EP (1) | EP3440226B1 (enExample) |
| JP (1) | JP6933858B2 (enExample) |
| CN (1) | CN109312398B (enExample) |
| AU (1) | AU2017246663B2 (enExample) |
| CA (1) | CA3020165A1 (enExample) |
| NZ (1) | NZ748011A (enExample) |
| SG (1) | SG11201808740TA (enExample) |
| WO (1) | WO2017176852A1 (enExample) |
| ZA (1) | ZA201807405B (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12221653B2 (en) | 2010-05-18 | 2025-02-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| CA2824387C (en) | 2011-02-09 | 2019-09-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| US20180173846A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| EP4428863A3 (en) | 2015-05-11 | 2024-12-11 | Natera, Inc. | Methods and compositions for determining ploidy |
| DK3443119T3 (da) | 2016-04-15 | 2022-05-23 | Natera Inc | Fremgangsmåde til bestemmelse af lunge cancer |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US12084720B2 (en) | 2017-12-14 | 2024-09-10 | Natera, Inc. | Assessing graft suitability for transplantation |
| CA3088891A1 (en) * | 2018-01-12 | 2019-07-18 | Natera, Inc. | Novel primers and uses thereof |
| JP7573443B2 (ja) | 2018-04-14 | 2024-10-25 | ナテラ， インコーポレイテッド | 循環腫瘍ｄｎａの個別化された検出を用いる癌検出およびモニタリングの方法 |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| CA3292164A1 (en) * | 2018-09-25 | 2025-11-29 | Co Diagnostics Inc | Allele-specific design of cooperative primers for improved nucleic acid variant genotyping |
| CN110964814B (zh) * | 2018-09-30 | 2024-05-03 | 迈克生物股份有限公司 | 用于核酸序列变异检测的引物、组合物及方法 |
| CN111349691B (zh) * | 2018-12-21 | 2023-10-20 | 迈克生物股份有限公司 | Egfr基因缺失突变检测用组合物、试剂盒及检测方法 |
| CN110541033B (zh) * | 2019-09-27 | 2023-11-14 | 迈克生物股份有限公司 | Egfr基因突变检测用组合物及检测方法 |
| CN110592215A (zh) * | 2019-09-27 | 2019-12-20 | 迈克生物股份有限公司 | 检测核酸序列的组合物及检测方法 |
| IL291819A (en) | 2019-10-02 | 2022-06-01 | Univ Rutgers | Assay methods and kits for detecting rare sequence variants |
| CN112824535B (zh) * | 2019-11-21 | 2023-07-04 | 迈克生物股份有限公司 | 基因突变多重检测用引物组合物及其试剂盒 |
| CN116121358B (zh) * | 2022-11-11 | 2024-07-23 | 重庆浦济生命科技有限公司 | 用于精神药物基因检测的组合物、试剂盒及使用方法 |
| EP4488384A1 (en) | 2023-07-05 | 2025-01-08 | Infiniplex Ltd. | Primers for selective amplification of rare target sequences |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7198897B2 (en) | 2001-12-19 | 2007-04-03 | Brandeis University | Late-PCR |
| US20060141518A1 (en) * | 2004-03-24 | 2006-06-29 | Lao Kai Q | Detection of gene expression |
| KR101433208B1 (ko) * | 2006-12-13 | 2014-08-22 | 루미넥스 코포레이션 | 실시간으로 ｐｃｒ의 다중 분석을 위한 시스템과 방법 |
| SG11201506146TA (en) * | 2013-02-07 | 2015-09-29 | Univ Rutgers | Highly selective nucleic acid amplification primers |
| WO2015038634A2 (en) * | 2013-09-13 | 2015-03-19 | Rutgers, The State University Of New Jersey | Multiplex diagnostic assay for lyme disease and other tick-borne diseases |
| GB201409786D0 (en) * | 2014-06-02 | 2014-07-16 | Primer Design Ltd | Nucleic acid amplification system |
| WO2016014921A1 (en) * | 2014-07-24 | 2016-01-28 | Brandies University | Linear-expo-linear pcr (lel-pcr) |
-
2017
- 2017-04-05 NZ NZ748011A patent/NZ748011A/en unknown
- 2017-04-05 EP EP17779722.2A patent/EP3440226B1/en active Active
- 2017-04-05 JP JP2018552172A patent/JP6933858B2/ja active Active
- 2017-04-05 AU AU2017246663A patent/AU2017246663B2/en active Active
- 2017-04-05 WO PCT/US2017/026088 patent/WO2017176852A1/en not_active Ceased
- 2017-04-05 CN CN201780035794.7A patent/CN109312398B/zh active Active
- 2017-04-05 CA CA3020165A patent/CA3020165A1/en active Pending
- 2017-04-05 US US16/091,824 patent/US11542547B2/en active Active
- 2017-04-05 SG SG11201808740TA patent/SG11201808740TA/en unknown
-
2018
- 2018-11-05 ZA ZA2018/07405A patent/ZA201807405B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP3440226A1 (en) | 2019-02-13 |
| EP3440226B1 (en) | 2021-02-17 |
| JP2019510501A (ja) | 2019-04-18 |
| NZ748011A (en) | 2025-05-02 |
| CN109312398B (zh) | 2022-05-31 |
| CN109312398A (zh) | 2019-02-05 |
| AU2017246663B2 (en) | 2022-09-01 |
| ZA201807405B (en) | 2021-04-28 |
| SG11201808740TA (en) | 2018-11-29 |
| AU2017246663A1 (en) | 2018-11-22 |
| WO2017176852A1 (en) | 2017-10-12 |
| US11542547B2 (en) | 2023-01-03 |
| EP3440226A4 (en) | 2019-11-13 |
| JP6933858B2 (ja) | 2021-09-08 |
| US20190225999A1 (en) | 2019-07-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220307 |
|
| EEER | Examination request |
Effective date: 20220307 |
|
| EEER | Examination request |
Effective date: 20220307 |
|
| EEER | Examination request |
Effective date: 20220307 |
|
| EEER | Examination request |
Effective date: 20220307 |