AU2017246663B2 - Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor - Google Patents

Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor Download PDF

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Publication number
AU2017246663B2
AU2017246663B2 AU2017246663A AU2017246663A AU2017246663B2 AU 2017246663 B2 AU2017246663 B2 AU 2017246663B2 AU 2017246663 A AU2017246663 A AU 2017246663A AU 2017246663 A AU2017246663 A AU 2017246663A AU 2017246663 B2 AU2017246663 B2 AU 2017246663B2
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sequence
primer
target
dna
mutant
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AU2017246663A1 (en
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Fred Russell Kramer
Salvatore A.E. Marras
Sanjay Tyagi
Diana VARGAS-GOLD
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Rutgers State University of New Jersey
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Rutgers State University of New Jersey
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2017246663A 2016-04-07 2017-04-05 Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor Active AU2017246663B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662319332P 2016-04-07 2016-04-07
US62/319,332 2016-04-07
PCT/US2017/026088 WO2017176852A1 (en) 2016-04-07 2017-04-05 Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor

Publications (2)

Publication Number Publication Date
AU2017246663A1 AU2017246663A1 (en) 2018-11-22
AU2017246663B2 true AU2017246663B2 (en) 2022-09-01

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AU2017246663A Active AU2017246663B2 (en) 2016-04-07 2017-04-05 Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor

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US (1) US11542547B2 (enExample)
EP (1) EP3440226B1 (enExample)
JP (1) JP6933858B2 (enExample)
CN (1) CN109312398B (enExample)
AU (1) AU2017246663B2 (enExample)
CA (1) CA3020165A1 (enExample)
NZ (1) NZ748011A (enExample)
SG (1) SG11201808740TA (enExample)
WO (1) WO2017176852A1 (enExample)
ZA (1) ZA201807405B (enExample)

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US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
CA2824387C (en) 2011-02-09 2019-09-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
EP4428863A3 (en) 2015-05-11 2024-12-11 Natera, Inc. Methods and compositions for determining ploidy
DK3443119T3 (da) 2016-04-15 2022-05-23 Natera Inc Fremgangsmåde til bestemmelse af lunge cancer
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
CA3088891A1 (en) * 2018-01-12 2019-07-18 Natera, Inc. Novel primers and uses thereof
JP7573443B2 (ja) 2018-04-14 2024-10-25 ナテラ， インコーポレイテッド 循環腫瘍ｄｎａの個別化された検出を用いる癌検出およびモニタリングの方法
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CA3292164A1 (en) * 2018-09-25 2025-11-29 Co Diagnostics Inc Allele-specific design of cooperative primers for improved nucleic acid variant genotyping
CN110964814B (zh) * 2018-09-30 2024-05-03 迈克生物股份有限公司 用于核酸序列变异检测的引物、组合物及方法
CN111349691B (zh) * 2018-12-21 2023-10-20 迈克生物股份有限公司 Egfr基因缺失突变检测用组合物、试剂盒及检测方法
CN110541033B (zh) * 2019-09-27 2023-11-14 迈克生物股份有限公司 Egfr基因突变检测用组合物及检测方法
CN110592215A (zh) * 2019-09-27 2019-12-20 迈克生物股份有限公司 检测核酸序列的组合物及检测方法
IL291819A (en) 2019-10-02 2022-06-01 Univ Rutgers Assay methods and kits for detecting rare sequence variants
CN112824535B (zh) * 2019-11-21 2023-07-04 迈克生物股份有限公司 基因突变多重检测用引物组合物及其试剂盒
CN116121358B (zh) * 2022-11-11 2024-07-23 重庆浦济生命科技有限公司 用于精神药物基因检测的组合物、试剂盒及使用方法
EP4488384A1 (en) 2023-07-05 2025-01-08 Infiniplex Ltd. Primers for selective amplification of rare target sequences

Citations (1)

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WO2014124290A1 (en) * 2013-02-07 2014-08-14 Rutgers, The State University Of New Jersey Highly selective nucleic acid amplification primers

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US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
US20060141518A1 (en) * 2004-03-24 2006-06-29 Lao Kai Q Detection of gene expression
KR101433208B1 (ko) * 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 ｐｃｒ의 다중 분석을 위한 시스템과 방법
WO2015038634A2 (en) * 2013-09-13 2015-03-19 Rutgers, The State University Of New Jersey Multiplex diagnostic assay for lyme disease and other tick-borne diseases
GB201409786D0 (en) * 2014-06-02 2014-07-16 Primer Design Ltd Nucleic acid amplification system
WO2016014921A1 (en) * 2014-07-24 2016-01-28 Brandies University Linear-expo-linear pcr (lel-pcr)

Patent Citations (1)

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WO2014124290A1 (en) * 2013-02-07 2014-08-14 Rutgers, The State University Of New Jersey Highly selective nucleic acid amplification primers

Also Published As

Publication number Publication date
EP3440226A1 (en) 2019-02-13
EP3440226B1 (en) 2021-02-17
JP2019510501A (ja) 2019-04-18
NZ748011A (en) 2025-05-02
CN109312398B (zh) 2022-05-31
CN109312398A (zh) 2019-02-05
ZA201807405B (en) 2021-04-28
SG11201808740TA (en) 2018-11-29
AU2017246663A1 (en) 2018-11-22
WO2017176852A1 (en) 2017-10-12
US11542547B2 (en) 2023-01-03
EP3440226A4 (en) 2019-11-13
JP6933858B2 (ja) 2021-09-08
US20190225999A1 (en) 2019-07-25
CA3020165A1 (en) 2017-10-12

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