CN109312398B - 能够检测密切相关的等位基因的多重核酸测定方法及其试剂 - Google Patents

能够检测密切相关的等位基因的多重核酸测定方法及其试剂 Download PDF

Info

Publication number
CN109312398B
CN109312398B CN201780035794.7A CN201780035794A CN109312398B CN 109312398 B CN109312398 B CN 109312398B CN 201780035794 A CN201780035794 A CN 201780035794A CN 109312398 B CN109312398 B CN 109312398B
Authority
CN
China
Prior art keywords
sequence
target
primer
dna
sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201780035794.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN109312398A (zh
Inventor
塞尔瓦托·A·E·马拉斯
D·瓦尔加斯-戈尔德
S·泰尔吉
F·R·克雷默
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers State University of New Jersey
Original Assignee
Rutgers State University of New Jersey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rutgers State University of New Jersey filed Critical Rutgers State University of New Jersey
Publication of CN109312398A publication Critical patent/CN109312398A/zh
Application granted granted Critical
Publication of CN109312398B publication Critical patent/CN109312398B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201780035794.7A 2016-04-07 2017-04-05 能够检测密切相关的等位基因的多重核酸测定方法及其试剂 Active CN109312398B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662319332P 2016-04-07 2016-04-07
US62/319,332 2016-04-07
PCT/US2017/026088 WO2017176852A1 (en) 2016-04-07 2017-04-05 Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor

Publications (2)

Publication Number Publication Date
CN109312398A CN109312398A (zh) 2019-02-05
CN109312398B true CN109312398B (zh) 2022-05-31

Family

ID=60000654

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780035794.7A Active CN109312398B (zh) 2016-04-07 2017-04-05 能够检测密切相关的等位基因的多重核酸测定方法及其试剂

Country Status (9)

Country Link
US (1) US11542547B2 (enExample)
EP (1) EP3440226B1 (enExample)
JP (1) JP6933858B2 (enExample)
CN (1) CN109312398B (enExample)
AU (1) AU2017246663B2 (enExample)
NZ (1) NZ748011A (enExample)
SG (1) SG11201808740TA (enExample)
WO (1) WO2017176852A1 (enExample)
ZA (1) ZA201807405B (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
DK3294906T3 (en) 2015-05-11 2024-08-05 Natera Inc Methods for determining ploidy
CN109477138A (zh) 2016-04-15 2019-03-15 纳特拉公司 肺癌检测方法
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
EP4245863A3 (en) * 2018-01-12 2024-01-24 Natera, Inc. Novel primers and uses thereof
CN112236535A (zh) 2018-04-14 2021-01-15 纳特拉公司 用于借助于循环肿瘤dna的个人化检测的癌症检测和监测的方法
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CA3292164A1 (en) * 2018-09-25 2025-11-29 Co Diagnostics Inc Allele-specific design of cooperative primers for improved nucleic acid variant genotyping
CN110964814B (zh) * 2018-09-30 2024-05-03 迈克生物股份有限公司 用于核酸序列变异检测的引物、组合物及方法
CN111349691B (zh) * 2018-12-21 2023-10-20 迈克生物股份有限公司 Egfr基因缺失突变检测用组合物、试剂盒及检测方法
CN110592215A (zh) * 2019-09-27 2019-12-20 迈克生物股份有限公司 检测核酸序列的组合物及检测方法
CN110541033B (zh) * 2019-09-27 2023-11-14 迈克生物股份有限公司 Egfr基因突变检测用组合物及检测方法
IL291819A (en) * 2019-10-02 2022-06-01 Univ Rutgers Assay methods and kits for detecting rare sequence variants
CN112824535B (zh) * 2019-11-21 2023-07-04 迈克生物股份有限公司 基因突变多重检测用引物组合物及其试剂盒
CN116121358B (zh) * 2022-11-11 2024-07-23 重庆浦济生命科技有限公司 用于精神药物基因检测的组合物、试剂盒及使用方法
EP4488384A1 (en) 2023-07-05 2025-01-08 Infiniplex Ltd. Primers for selective amplification of rare target sequences

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105102467A (zh) * 2013-02-07 2015-11-25 新泽西鲁特格斯州立大学 高度选择性核酸扩增引物
WO2015185902A1 (en) * 2014-06-02 2015-12-10 Primer Design Ltd Nucleic acid amplification system

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
US20060141518A1 (en) * 2004-03-24 2006-06-29 Lao Kai Q Detection of gene expression
CN102851369B (zh) 2006-12-13 2015-01-21 卢米耐克斯公司 用于实时pcr多重分析的系统和方法
CA2960776C (en) 2013-09-13 2021-11-16 Rutgers, The State University Of New Jersey Multiplex diagnostic assay for lyme disease and other tick-borne diseases
WO2016014921A1 (en) * 2014-07-24 2016-01-28 Brandies University Linear-expo-linear pcr (lel-pcr)

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105102467A (zh) * 2013-02-07 2015-11-25 新泽西鲁特格斯州立大学 高度选择性核酸扩增引物
WO2015185902A1 (en) * 2014-06-02 2015-12-10 Primer Design Ltd Nucleic acid amplification system

Also Published As

Publication number Publication date
ZA201807405B (en) 2021-04-28
EP3440226A4 (en) 2019-11-13
EP3440226A1 (en) 2019-02-13
AU2017246663B2 (en) 2022-09-01
SG11201808740TA (en) 2018-11-29
US11542547B2 (en) 2023-01-03
JP2019510501A (ja) 2019-04-18
JP6933858B2 (ja) 2021-09-08
NZ748011A (en) 2025-05-02
CA3020165A1 (en) 2017-10-12
CN109312398A (zh) 2019-02-05
WO2017176852A1 (en) 2017-10-12
AU2017246663A1 (en) 2018-11-22
US20190225999A1 (en) 2019-07-25
EP3440226B1 (en) 2021-02-17

Similar Documents

Publication Publication Date Title
CN109312398B (zh) 能够检测密切相关的等位基因的多重核酸测定方法及其试剂
CN110195097B (zh) 高度选择性核酸扩增引物
JP7744025B2 (ja) 希少配列変異体を検出するためのアッセイ方法およびキット
US20160040242A1 (en) Methods and Kits for Detecting Nucleic Acid Mutants in Wild-Type Populations
KR102377229B1 (ko) 표적 핵산 및 변이체의 검출
EP3167060B1 (en) Dna amplification technology
CA2865163A1 (en) Multimodal pcr target detection
EP3055430B1 (en) Method for the detection of target nucleic acid sequences
KR20160106041A (ko) Ｎｒａｓ 및 ｂｒａｆ 핵산의 멀티플렉스 분석을 위한 조성물 및 방법
CA3020165C (en) Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor
CA3150825C (en) Assay methods and kits for detecting rare sequence variants
WO2025198958A1 (en) Use of variant-signaling primers for the detection of genetic rearrangements and modifications
US20080233578A1 (en) Method for detecting mutation of nucleic acid using single-stranded dna-binding protein
WO2025008372A1 (en) Primers for selective amplification of rare target sequences
EP2208797A2 (en) Methods, compositions and kits for use in polynucleotide amplification
US20060199188A1 (en) Methods, compositions and kits for use in polynucleotide amplification

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant