CA2933343A1 - Compositions and methods for detecting rare sequence variants - Google Patents

Compositions and methods for detecting rare sequence variants Download PDF

Info

Publication number
CA2933343A1
CA2933343A1 CA2933343A CA2933343A CA2933343A1 CA 2933343 A1 CA2933343 A1 CA 2933343A1 CA 2933343 A CA2933343 A CA 2933343A CA 2933343 A CA2933343 A CA 2933343A CA 2933343 A1 CA2933343 A1 CA 2933343A1
Authority
CA
Canada
Prior art keywords
sequence
polynucleotides
primer
amplification
cftr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA2933343A
Other languages
English (en)
French (fr)
Inventor
Shengrong LIN
Zhaohui Sun
Grace Qizhi ZHAO
Paul Ling-Fung TANG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Accuragen Holdings Ltd
Original Assignee
Accuragen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Accuragen Inc filed Critical Accuragen Inc
Publication of CA2933343A1 publication Critical patent/CA2933343A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/307—Circular oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/131—Inverse PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2933343A 2013-12-11 2014-12-11 Compositions and methods for detecting rare sequence variants Pending CA2933343A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361914907P 2013-12-11 2013-12-11
US61/914,907 2013-12-11
US201461987414P 2014-05-01 2014-05-01
US61/987,414 2014-05-01
US201462010975P 2014-06-11 2014-06-11
US62/010,975 2014-06-11
PCT/US2014/069848 WO2015089333A1 (en) 2013-12-11 2014-12-11 Compositions and methods for detecting rare sequence variants

Publications (1)

Publication Number Publication Date
CA2933343A1 true CA2933343A1 (en) 2015-06-18

Family

ID=53371852

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2933343A Pending CA2933343A1 (en) 2013-12-11 2014-12-11 Compositions and methods for detecting rare sequence variants

Country Status (12)

Country Link
US (4) US10767222B2 (OSRAM)
EP (2) EP3495506B1 (OSRAM)
JP (1) JP6435334B2 (OSRAM)
KR (3) KR102640585B1 (OSRAM)
CN (2) CN104946737B (OSRAM)
AU (3) AU2014362227B2 (OSRAM)
CA (1) CA2933343A1 (OSRAM)
ES (2) ES2707744T3 (OSRAM)
IL (3) IL300974A (OSRAM)
MX (1) MX376900B (OSRAM)
SG (2) SG10201807532SA (OSRAM)
WO (1) WO2015089333A1 (OSRAM)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017037657A1 (en) * 2015-09-02 2017-03-09 Robert Osborne Method of identifying sequence variants using concatenation
CN110872632A (zh) * 2018-08-30 2020-03-10 深圳华大生命科学研究院 咽颊炎链球菌的特异基因序列、其检测引物及其应用

Families Citing this family (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP1984738A2 (en) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2047910B1 (en) 2006-05-11 2012-01-11 Raindance Technologies, Inc. Microfluidic device and method
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP2318552B1 (en) 2008-09-05 2016-11-23 TOMA Biosciences, Inc. Methods for stratifying and annotating cancer drug treatment options
EP3415235B1 (en) 2009-03-23 2025-11-12 Bio-Rad Laboratories, Inc. Manipulation of microfluidic droplets
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
JP5986572B2 (ja) 2010-09-24 2016-09-06 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 固定化プライマーを使用した標的ｄｎａの直接的な捕捉、増幅、および配列決定
EP2622103B2 (en) 2010-09-30 2022-11-16 Bio-Rad Laboratories, Inc. Sandwich assays in droplets
EP2673614B1 (en) 2011-02-11 2018-08-01 Raindance Technologies, Inc. Method for forming mixed droplets
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US9556470B2 (en) 2011-06-02 2017-01-31 Raindance Technologies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US10691775B2 (en) 2013-01-17 2020-06-23 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
US9679104B2 (en) 2013-01-17 2017-06-13 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
GB2523495A (en) 2013-01-17 2015-08-26 Edico Genome Corp Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
US10068054B2 (en) 2013-01-17 2018-09-04 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
US10847251B2 (en) 2013-01-17 2020-11-24 Illumina, Inc. Genomic infrastructure for on-site or cloud-based DNA and RNA processing and analysis
US9792405B2 (en) 2013-01-17 2017-10-17 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
CN104946737B (zh) 2013-12-11 2019-02-22 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US9944977B2 (en) * 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
EP3795697B1 (en) 2014-02-04 2024-09-18 Jumpcode Genomics, Inc. Genome fractioning
ES2935285T3 (es) 2014-06-06 2023-03-03 Univ Cornell Método para identificación y enumeración de cambios en la secuencia de ácidos nucleicos, expresión, copia, o metilación de ADN, usando reacciones combinadas de nucleasa, ligasa, polimerasa y secuenciación
US20160048608A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
EP3224595A4 (en) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
US11987836B2 (en) * 2014-09-30 2024-05-21 Global Life Sciences Solutions Usa Llc Method for nucleic acid analysis directly from an unpurified biological sample
HK1255431A1 (zh) 2015-03-23 2019-08-16 Edico Genome Corporation 用於基因组可视化的方法和系统
TWI728994B (zh) * 2015-08-12 2021-06-01 香港中文大學 血漿ｄｎａ之單分子定序
CN108603190B (zh) 2015-09-08 2023-05-23 美国冷泉港实验室 使用经破碎的核苷酸的高通量多重测序确定基因拷贝数
AU2016334233B2 (en) 2015-10-09 2023-01-05 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3365335B1 (en) 2015-10-23 2024-02-14 Array Biopharma, Inc. 2-aryl- and 2-heteroaryl-substituted 2-pyridazin-3(2h)-one compounds as inhibitors of fgfr tyrosine kinases
EP3383994A4 (en) * 2015-12-03 2019-08-28 Accuragen Holdings Limited METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS
US20170270245A1 (en) 2016-01-11 2017-09-21 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods for performing secondary and/or tertiary processing
US10068183B1 (en) 2017-02-23 2018-09-04 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods executed on a quantum processing platform
WO2017136720A1 (en) * 2016-02-05 2017-08-10 Good Start Genetics, Inc. Variant detection of sequencing assays
US9976181B2 (en) 2016-03-25 2018-05-22 Karius, Inc. Synthetic nucleic acid spike-ins
WO2017190067A1 (en) 2016-04-28 2017-11-02 Impact Genomics, Inc. Methods of assessing and monitoring tumor load
US10354747B1 (en) * 2016-05-06 2019-07-16 Verily Life Sciences Llc Deep learning analysis pipeline for next generation sequencing
EP3458586B1 (en) 2016-05-16 2022-12-28 Accuragen Holdings Limited Method of improved sequencing by strand identification
WO2017209891A1 (en) 2016-05-31 2017-12-07 Quantapore, Inc. Two-color nanopore sequencing
AU2017277929A1 (en) * 2016-06-10 2018-12-13 Zinfandel Pharmaceuticals, Inc. Methods for detecting structural variants in neurodegenerative disease
US11441169B2 (en) 2016-06-17 2022-09-13 Ludwig Institute For Cancer Research Ltd Methods of small-RNA transcriptome sequencing and applications thereof
EP3475449B1 (en) * 2016-06-23 2022-08-17 Accuragen Holdings Limited Uses of a cell-free nucleic acid standards
AU2017290237B2 (en) 2016-06-30 2020-10-22 GRAIL, Inc Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library
CN107557355A (zh) * 2016-07-01 2018-01-09 Pgi股份有限公司 建构环状模板和检测dna分子的方法
ES2875318T3 (es) 2016-07-18 2021-11-10 Hoffmann La Roche Procedimiento para generar colecciones de ADN circular monocatenario para secuenciación de molécula única
US10626443B2 (en) * 2016-08-10 2020-04-21 Grail, Inc. Methods of analyzing nucleic acid fragments
CN106282161B (zh) * 2016-08-12 2020-10-30 成都诺恩基因科技有限公司 特异捕获并重复复制低频率dna碱基变异的方法及应用
WO2018035170A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
JP6810559B2 (ja) * 2016-09-09 2021-01-06 株式会社日立製作所 環状型一本鎖核酸、およびその調製方法と使用方法
CN110023504B (zh) * 2016-09-15 2023-05-09 阿谢尔德克斯有限责任公司 用于分析无细胞dna的核酸样品制备方法
AU2017328950B2 (en) 2016-09-15 2023-09-14 Archerdx, Llc Methods of nucleic acid sample preparation
WO2018077847A1 (en) * 2016-10-31 2018-05-03 F. Hoffmann-La Roche Ag Barcoded circular library construction for identification of chimeric products
EP3612642B1 (en) * 2017-04-17 2025-07-09 GeneFirst Limited Methods and kits for preparing nucleic acid libraries
EP3643787A4 (en) * 2017-06-20 2021-01-06 MGI Tech Co., Ltd. PCR PRIMER PAIR AND USE OF IT
EP3662480A4 (en) * 2017-08-04 2021-05-19 BillionToOne, Inc. MOLECULES ASSOCIATED WITH A TARGET FOR A CHARACTERIZATION ASSOCIATED WITH BIOLOGICAL TARGETS
AU2018342007B2 (en) * 2017-08-07 2025-07-17 The Johns Hopkins University Methods and materials for assessing and treating cancer
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
WO2019060722A2 (en) 2017-09-22 2019-03-28 X Gen Us Co. METHODS AND COMPOSITIONS FOR USE IN PREPARING POLYNUCLEOTIDES
JP7067896B2 (ja) * 2017-10-27 2022-05-16 シスメックス株式会社 品質評価方法、品質評価装置、プログラム、および記録媒体
WO2019086531A1 (en) * 2017-11-03 2019-05-09 F. Hoffmann-La Roche Ag Linear consensus sequencing
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
WO2019191900A1 (en) 2018-04-03 2019-10-10 Burning Rock Biotech Compositions and methods for preparing nucleic acid libraries
CN113383083B (zh) 2018-04-27 2024-12-10 赛纳生物科技(北京)有限公司 用于制备多核苷酸的方法和组合物
CN112534063A (zh) 2018-05-22 2021-03-19 安序源有限公司 用于核酸测序的方法、系统和组合物
WO2019241290A1 (en) * 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products
CN112654713A (zh) * 2018-07-05 2021-04-13 安可济控股有限公司 用于数字聚合酶链反应的组合物和方法
SG11202100564PA (en) * 2018-07-23 2021-02-25 Univ Hong Kong Chinese Cell-free dna damage analysis and its clinical applications
CA3118990A1 (en) 2018-11-21 2020-05-28 Karius, Inc. Direct-to-library methods, systems, and compositions
WO2020131923A1 (en) * 2018-12-17 2020-06-25 uBiome, Inc. Automatic standardization pipeline for data analysis
EP3898626A1 (en) 2018-12-19 2021-10-27 Array Biopharma, Inc. Substituted pyrazolo[1,5-a]pyridine compounds as inhibitors of fgfr tyrosine kinases
EP3898615A1 (en) 2018-12-19 2021-10-27 Array Biopharma, Inc. 7-((3,5-dimethoxyphenyl)amino)quinoxaline derivatives as fgfr inhibitors for treating cancer
CN113728116A (zh) * 2019-02-22 2021-11-30 安可济控股有限公司 用于早期癌症检测的方法和组合物
US11512346B2 (en) * 2019-03-14 2022-11-29 Genome Research Limited Method for sequencing a direct repeat
EP4017620B1 (en) * 2019-08-21 2024-05-01 Life Technologies Corporation System and method for sequencing
CA3154235A1 (en) * 2019-09-13 2021-03-18 University Health Network Detection of circulating tumor dna using double stranded hybrid capture
EP3798319A1 (en) 2019-09-30 2021-03-31 Diagenode S.A. An improved diagnostic and/or sequencing method and kit
EP3828283A1 (en) * 2019-11-28 2021-06-02 Diagenode S.A. An improved sequencing method and kit
CA3158739A1 (en) * 2019-11-19 2021-05-27 Xiao Bing Wang Method for identifying gene fusions by circle cdna amplification
US20240279735A1 (en) * 2019-12-20 2024-08-22 Korea University Research And Business Foundation Primers for detecting trace amount of rare single- nucleotide variant and method for specifically and sensitively detecting trace amount of rare single-nucleotide variant by using same
US20230115945A1 (en) 2019-12-30 2023-04-13 Tyra Biosciences, Inc. Aminopyrimidine compounds
US12441707B2 (en) 2019-12-30 2025-10-14 Tyra Biosciences, Inc. Indazole compounds
AU2020423361B2 (en) * 2020-01-17 2024-08-29 Mgi Tech Co., Ltd. Method for synchronously sequencing sense strand and antisense strand of DNA
CA3182741A1 (en) * 2020-06-15 2021-12-23 Aziz AL'KHAFAJI Chimeric amplicon array sequencing
WO2022018055A1 (en) * 2020-07-20 2022-01-27 Westfälische Wilhelms-Universität Münster Circulation method to sequence immune repertoires of individual cells
WO2022087485A1 (en) * 2020-10-22 2022-04-28 Singular Genomics Systems, Inc. Nucleic acid circularization and amplification on a surface
MX2023007793A (es) 2020-12-30 2023-09-22 Tyra Biosciences Inc Compuestos de indazol como inhibidores de cinasas.
CN115956129A (zh) * 2021-02-03 2023-04-11 国立大学法人东北大学 遗传信息分析系统和遗传信息分析方法
EP4047100A1 (en) * 2021-02-18 2022-08-24 Miltenyi Biotec B.V. & Co. KG Ngs targeted dna panel using multiplex selective rca
MX2023009954A (es) 2021-02-26 2023-11-09 Tyra Biosciences Inc Compuestos de aminopirimidina y métodos de uso de estos.
US20250188505A1 (en) * 2021-03-26 2025-06-12 Flagship Pioneering Innovations Vii, Llc Production of circular polyribonucleotides in a prokaryotic system
EP4314281A1 (en) * 2021-03-26 2024-02-07 Flagship Pioneering Innovations VII, LLC Production of circular polyribonucleotides in a eukaryotic system
AR125217A1 (es) * 2021-03-26 2023-06-28 Flagship Pioneering Innovations Vii Llc Producción de polirribonucleótidos circulares en un sistema procariota
EP4314325A4 (en) * 2021-03-31 2025-03-05 Jumpcode Genomics, Inc. Methods for targeted nucleic acid sequencing
CN113549674B (zh) * 2021-04-22 2023-11-03 福建和瑞基因科技有限公司 一种检测样本中目标序列整合和突变的方法及其引物的设计方法和试剂盒
WO2023055752A2 (en) 2021-09-28 2023-04-06 Axbio Inc. Methods for processing a nucleic acid sample and compositions thereof
US12431218B2 (en) 2022-03-08 2025-09-30 Illumina, Inc. Multi-pass software-accelerated genomic read mapping engine
WO2024006897A1 (en) 2022-06-29 2024-01-04 Tyra Biosciences, Inc. Indazole compounds
EP4547670A1 (en) 2022-06-29 2025-05-07 Tyra Biosciences, Inc. Polymorphic compounds and uses thereof
US20240068010A1 (en) * 2022-08-31 2024-02-29 Genomill Health Oy Highly sensitive methods for accurate parallel quantification of variant nucleic acids
WO2024138112A1 (en) 2022-12-22 2024-06-27 Tyra Biosciences, Inc. Indazole compounds
WO2025129014A1 (en) 2023-12-15 2025-06-19 Tyra Biosciences, Inc. Indazole compounds for the treatment of cancer
WO2025132779A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025222097A1 (en) 2024-04-19 2025-10-23 Tyra Biosciences, Inc. Indazole-based protac degraders and their anticancer activity

Family Cites Families (167)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US6677312B1 (en) 1989-03-29 2004-01-13 The Johns Hopkins University Methods for restoring wild-type p53 gene function
US5362623A (en) 1991-06-14 1994-11-08 The John Hopkins University Sequence specific DNA binding by p53
DE69032864T3 (de) 1989-03-29 2013-01-31 The Johns Hopkins University Nachweis des Ausfalls des Wildtyps des p53-Gens
US6800617B1 (en) 1989-03-29 2004-10-05 The Johns Hopkins University Methods for restoring wild-type p53 gene function
US5527676A (en) 1989-03-29 1996-06-18 The Johns Hopkins University Detection of loss of the wild-type P53 gene and kits therefor
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
DE69028257T2 (de) 1989-07-11 1997-01-09 Gen Probe Inc Verfahren zur Vervielfältigung von Nukleinsäuresequenzen
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5527670A (en) 1990-09-12 1996-06-18 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
CA2100472C (en) 1991-01-16 2009-04-07 Kenneth W. Kinzler Inherited and somatic mutations of apc gene in colerectal cancer of humans
US5352775A (en) 1991-01-16 1994-10-04 The Johns Hopkins Univ. APC gene and nucleic acid probes derived therefrom
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5330892A (en) 1991-03-13 1994-07-19 The Johns Hopkins University MCC gene (mutated in colorectal cancer) used for diagnosis of cancer in humans
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US7326778B1 (en) 1993-05-05 2008-02-05 The John Hopkins University Mutator gene and hereditary non-polyposis colorectal cancer
US5492808A (en) 1993-05-05 1996-02-20 The Johns Hopkins University Means for detecting familial colon cancer (FCC)
US5591826A (en) 1993-05-05 1997-01-07 The Johns Hopkins University Human MSH2 protein
EP0702728B1 (en) 1993-06-09 1998-04-15 Gamera Bioscience Corporation Magnetic cycle reaction
WO1995013375A1 (en) 1993-11-10 1995-05-18 The Johns Hopkins University Tumor suppressor waf1
WO1995015381A2 (en) 1993-12-02 1995-06-08 The Johns Hopkins University HUMAN MUTATOR GENE hMSH2 AND HEREDITARY NON POLYPOSIS COLORECTAL CANCER
US6482606B1 (en) 1994-01-27 2002-11-19 Human Genome Sciences, Inc. Human DNA mismatch repair polynucleotides
US6380369B1 (en) 1994-01-27 2002-04-30 Human Genome Sciences, Inc. Human DNA mismatch repair proteins
EP0749496A4 (en) 1994-01-27 1999-03-03 Human Genome Sciences Inc HUMAN DNA MALFUNCTION REPAIR PROTEINS
JPH09510107A (ja) 1994-03-15 1997-10-14 サイエンティフィック ジェネリクス リミテッド 二本鎖核酸の電気化学的変性
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6593086B2 (en) 1996-05-20 2003-07-15 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
DE69612013T2 (de) 1995-11-21 2001-08-02 Yale University, New Haven Unimolekulare segmentamplifikation und bestimmung
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6156504A (en) 1996-03-15 2000-12-05 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
US8048629B2 (en) 1996-03-15 2011-11-01 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
US6511805B1 (en) 1996-03-15 2003-01-28 The Penn State Research Foundation Methods for detecting papillomavirus DNA in blood plasma and serum
PT938320E (pt) 1996-03-26 2010-09-22 Michael S Kopreski Método que permite a utilização de arn extracelular extraído de plasma ou de soro para detectar, monitorizar ou avaliar o cancro
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
GB9706654D0 (en) 1997-04-02 1997-05-21 Scient Generics Ltd Disassociation of interacting molecules
EP0917590A1 (en) 1997-04-04 1999-05-26 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
WO1999053102A1 (en) 1998-04-16 1999-10-21 Packard Bioscience Company Analysis of polynucleotide sequence
DE69940623D1 (de) 1998-11-09 2009-04-30 Eiken Chemical Prozess zur Synthetisierung von Nukleinsäure
GB9903906D0 (en) 1999-02-19 1999-04-14 Microbiological Res Authority Method and apparatus for nucleic acid strand separation
WO2000055369A1 (en) 1999-03-17 2000-09-21 The Board Of Trustees Of The Leland Stanford Junior University Mismatch repair detection
JP2002541799A (ja) * 1999-04-12 2002-12-10 ジェネンテック・インコーポレーテッド Ｐｃｒによるクローニング方法
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
WO2001018230A1 (en) 1999-09-08 2001-03-15 University Of Victoria Innovation & Development Corporation Use of psychrotrophic bacterium in biotechnology applications
NZ517121A (en) 1999-09-13 2004-05-28 Nugen Technologies Inc Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
AU783841B2 (en) * 1999-11-26 2005-12-15 454 Life Sciences Corporation Nucleic acid probe arrays
US6653079B2 (en) 2000-03-13 2003-11-25 Freshgene, Inc. Methods for detection of differences in nucleic acids
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US6626906B1 (en) 2000-10-23 2003-09-30 Sdgi Holdings, Inc. Multi-planar adjustable connector
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
US20040067511A1 (en) 2001-02-27 2004-04-08 Thomas David C. Circular probe amplification (cpa) using energy-transfer primers
BR0205268A (pt) 2001-03-09 2004-11-30 Nugen Technologies Inc Processos e composições para a mplificação de sequências de rna
US7122715B2 (en) 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US6815167B2 (en) 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
WO2004027025A2 (en) 2002-09-20 2004-04-01 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
CA2505472A1 (en) 2002-11-11 2004-05-27 Affymetrix, Inc. Methods for identifying dna copy number changes
US7309571B2 (en) 2003-02-03 2007-12-18 Ge Healthcare Bio-Sciences Corp. Amplification of self-ligated, circularized cDNA for expression profiling
WO2005059157A2 (en) 2003-12-11 2005-06-30 The Board Of Trustees Of The Leland Stanford Junior University METHODS AND COMPOSITIONS FOR USE IN PREPARING HAIRPIN RNAs
JP2007525571A (ja) 2004-01-07 2007-09-06 ソレクサ リミテッド 修飾分子アレイ
JP2007526772A (ja) 2004-02-27 2007-09-20 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ インサイチュー配列決定用ポロニー蛍光ビーズ
WO2005095654A1 (en) 2004-03-25 2005-10-13 Biohelix Corporation Helicase-dependent amplification of circular nucleic acids
WO2005113824A2 (en) 2004-05-14 2005-12-01 Zhenghe Wang Protein tyrosine phosphatase mutations in cancers
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US8916351B2 (en) 2005-01-04 2014-12-23 Hitachi Chemical Co., Ltd. Primer generation rolling circle amplification
WO2006084051A2 (en) 2005-02-01 2006-08-10 John Wayne Cancer Institute Use of id4 for diagnosis and treatment of cancer
WO2006087574A2 (en) 2005-02-19 2006-08-24 Geneform Technologies Limited Isothermal nucleic acid amplification
EP1712639B1 (en) 2005-04-06 2008-08-27 Maurice Stroun Method for the diagnosis of cancer by detecting circulating DNA and RNA
US9139849B2 (en) 2005-04-08 2015-09-22 The United States of America as Represented by the Government of the Department of Health and Human Services Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
CA2604095A1 (en) * 2005-04-12 2006-10-19 454 Life Sciences Corporation Methods for determining sequence variants using ultra-deep sequencing
US20060228721A1 (en) * 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
JP5331476B2 (ja) 2005-06-15 2013-10-30 カリダ・ジェノミックス・インコーポレイテッド 遺伝子解析および化学解析用の単分子アレイ
WO2007024653A2 (en) 2005-08-19 2007-03-01 Bioventures, Inc. Method and substances for isolating micro rnas
AU2007249635B2 (en) * 2005-10-07 2012-05-31 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
AU2006320739B2 (en) 2005-11-28 2012-03-29 Pacific Biosciences Of California, Inc. Uniform surfaces for hybrid material substrates and methods for making and using same
US7537897B2 (en) 2006-01-23 2009-05-26 Population Genetics Technologies, Ltd. Molecular counting
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
WO2007117832A2 (en) 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
WO2007133703A2 (en) 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US8768629B2 (en) * 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
EP3617321B1 (en) 2006-05-31 2024-10-23 Sequenom, Inc. Kit for the extraction and amplification of nucleic acid from a sample
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
AU2007203390B2 (en) 2007-04-26 2012-10-04 Yew Jin Fong Improved lace fastener
FR2924440B1 (fr) 2007-12-04 2015-01-09 Pf Medicament Nouveau procede de generation et de criblage d'une banque d'anticorps
ES2805347T3 (es) * 2009-02-11 2021-02-11 Caris Mpi Inc Perfilado molecular de tumores
CA2751470C (en) 2009-02-16 2016-07-26 Epicentre Technologies Corporation Template-independent ligation of single-stranded dna
WO2010117817A2 (en) 2009-03-30 2010-10-14 Life Technologies Corporation Methods for generating target specific probes for solution based capture
WO2010115100A1 (en) * 2009-04-03 2010-10-07 L&C Diagment, Inc. Multiplex nucleic acid detection methods and systems
US8962253B2 (en) * 2009-04-13 2015-02-24 Somagenics Inc. Methods and compositions for detection of small RNAs
GB0910302D0 (en) 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
US20110237444A1 (en) 2009-11-20 2011-09-29 Life Technologies Corporation Methods of mapping genomic methylation patterns
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8801578B2 (en) 2009-12-21 2014-08-12 Core Industries, Llc Instructional displays and methods for exercise machine
MX2012007305A (es) * 2009-12-22 2012-07-04 Procter & Gamble Composicion liquida de limpieza y/o lavado.
WO2011120046A2 (en) 2010-03-26 2011-09-29 Swift Biosciences, Inc. Methods and compositions for isolating polynucleotides
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
EP2585593B1 (en) 2010-06-24 2017-03-01 Population Genetics Technologies Ltd. Methods for polynucleotide library production, immortalization and region of interest extraction
WO2012006291A2 (en) 2010-07-06 2012-01-12 Life Technologies Corporation Systems and methods to detect copy number variation
CN102971434B (zh) 2010-08-11 2014-04-09 中国科学院心理研究所 一种甲基化dna的高通量测序方法及其应用
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
CN101985654A (zh) * 2010-10-21 2011-03-16 徐州医学院 检测多样本多位点snp的检测方法
US9260753B2 (en) * 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
CN110016499B (zh) 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序系统
US9670527B2 (en) 2011-06-29 2017-06-06 The Johns Hopkins University Enrichment of nucleic acids by complimentary capture
EP2740804A4 (en) 2011-08-05 2015-06-24 Toshiba Kk PROCESS FOR NUCLEIC ACID ANALYSIS
US9586987B2 (en) 2011-09-08 2017-03-07 Kabushiki Kaisha Dnaform Primer set for isothermal amplication of a target nucleic acid sequence
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
WO2013059740A1 (en) 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
WO2013074632A1 (en) 2011-11-14 2013-05-23 Rose Floyd D Mismatch nucleotide purification and identification
US20130217023A1 (en) * 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
PT2828218T (pt) 2012-03-20 2020-11-11 Univ Washington Through Its Center For Commercialization Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
EP2855707B1 (en) 2012-05-31 2017-07-12 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
US10538807B2 (en) 2012-06-01 2020-01-21 Fred Hutchinson Cancer Research Center Compositions and methods for detecting rare nucleic acid molecule mutations
CA2875666A1 (en) 2012-06-14 2013-12-19 Fred Hutchinson Cancer Research Center Compositions and methods for sensitive mutation detection in nucleic acid molecules
US9540687B2 (en) 2012-07-11 2017-01-10 Berry Genomics Co., Ltd. DNA fragment detection method, DNA fragment detection kit and the use thereof
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
AU2013292610B2 (en) 2012-07-17 2018-05-17 Myriad Women's Health, Inc. System and methods for detecting genetic variation
EP2877594B1 (en) 2012-07-20 2019-12-04 Verinata Health, Inc. Detecting and classifying copy number variation in a fetal genome
US20140066317A1 (en) 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP2914753A4 (en) 2012-10-31 2016-06-08 Becton Dickinson Co SELECTIVE AMPLIFICATION AND REAL-TIME PCR DETECTION OF RARE MUTATIONS
US10980804B2 (en) 2013-01-18 2021-04-20 Foundation Medicine, Inc. Methods of treating cholangiocarcinoma
EP3550033B1 (en) 2013-02-20 2021-10-20 Emory University Compositions for sequencing nucleic acids in mixtures
EP2971097B1 (en) 2013-03-15 2018-08-01 Verinata Health, Inc Generating cell-free dna libraries directly from blood
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
CN105518151B (zh) 2013-03-15 2021-05-25 莱兰斯坦福初级大学评议会 循环核酸肿瘤标志物的鉴别和用途
US10081825B2 (en) 2013-03-15 2018-09-25 Aegea Biotechnologies, Inc. Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
US20150033372A1 (en) 2013-05-01 2015-01-29 Kymab Limited Human VpreB & Chimaeric Surrogate Light Chains in Transgenic Non-Human Vertebrates
US9644232B2 (en) 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
WO2015048535A1 (en) 2013-09-27 2015-04-02 Natera, Inc. Prenatal diagnostic resting standards
EP3760733A1 (en) 2013-10-17 2021-01-06 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
KR20240038168A (ko) 2013-11-07 2024-03-22 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 인간 마이크로바이옴 및 그의 성분의 분석을 위한 무세포 핵산
GB201321123D0 (en) 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CN104946737B (zh) 2013-12-11 2019-02-22 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CN111534580B (zh) 2013-12-28 2024-06-04 夸登特健康公司 用于检测遗传变异的方法和系统
CN104745679B (zh) 2013-12-31 2018-06-15 杭州贝瑞和康基因诊断技术有限公司 一种无创检测egfr基因突变的方法及试剂盒
EP3099796B1 (en) 2014-01-31 2018-08-29 Swift Biosciences, Inc. Improved methods for processing dna substrates
ES2935285T3 (es) 2014-06-06 2023-03-03 Univ Cornell Método para identificación y enumeración de cambios en la secuencia de ácidos nucleicos, expresión, copia, o metilación de ADN, usando reacciones combinadas de nucleasa, ligasa, polimerasa y secuenciación
CA3172086A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
US10364458B2 (en) 2014-07-16 2019-07-30 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
US11987836B2 (en) 2014-09-30 2024-05-21 Global Life Sciences Solutions Usa Llc Method for nucleic acid analysis directly from an unpurified biological sample
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
AU2016334233B2 (en) 2015-10-09 2023-01-05 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3383994A4 (en) 2015-12-03 2019-08-28 Accuragen Holdings Limited METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS
EP3458586B1 (en) 2016-05-16 2022-12-28 Accuragen Holdings Limited Method of improved sequencing by strand identification
US10370701B2 (en) 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
EP3475449B1 (en) 2016-06-23 2022-08-17 Accuragen Holdings Limited Uses of a cell-free nucleic acid standards
WO2018035170A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017037657A1 (en) * 2015-09-02 2017-03-09 Robert Osborne Method of identifying sequence variants using concatenation
US10519488B2 (en) 2015-09-02 2019-12-31 Oxford Nanopore Technologies, Ltd. Method of identifying sequence variants using concatenation
US11111524B2 (en) 2015-09-02 2021-09-07 Oxford Nanopore Technologies, Ltd. Method of identifying sequence variants using concatenation
US12104202B2 (en) 2015-09-02 2024-10-01 Oxford Nanopore Technologies Plc Method of identifying sequence variants using concatenation
CN110872632A (zh) * 2018-08-30 2020-03-10 深圳华大生命科学研究院 咽颊炎链球菌的特异基因序列、其检测引物及其应用

Also Published As

Publication number Publication date
SG11201604737VA (en) 2016-07-28
WO2015089333A1 (en) 2015-06-18
KR102379877B1 (ko) 2022-03-30
MX2016007605A (es) 2017-01-13
EP3080298A4 (en) 2017-07-05
US20210054449A1 (en) 2021-02-25
JP6435334B2 (ja) 2018-12-05
AU2024201195A1 (en) 2024-03-14
EP3495506A1 (en) 2019-06-12
KR102640585B1 (ko) 2024-02-23
HK1214843A1 (zh) 2016-08-05
JP2017510244A (ja) 2017-04-13
ES2707744T3 (es) 2019-04-04
US20160304954A1 (en) 2016-10-20
US12234512B2 (en) 2025-02-25
CN104946737B (zh) 2019-02-22
IL274464A (en) 2020-06-30
IL300974A (en) 2023-04-01
IL274464B1 (en) 2023-04-01
SG10201807532SA (en) 2018-09-27
KR20220064973A (ko) 2022-05-19
AU2021206868A1 (en) 2021-08-12
IL274464B2 (en) 2023-08-01
US11597973B2 (en) 2023-03-07
ES2960338T3 (es) 2024-03-04
IL246021B (en) 2020-06-30
US20250290136A1 (en) 2025-09-18
CN109706222A (zh) 2019-05-03
US20230416819A1 (en) 2023-12-28
MX376900B (es) 2025-03-04
AU2014362227A1 (en) 2016-06-23
IL246021A0 (en) 2016-07-31
US10767222B2 (en) 2020-09-08
AU2014362227B2 (en) 2021-05-13
KR20160106596A (ko) 2016-09-12
EP3080298A1 (en) 2016-10-19
CN104946737A (zh) 2015-09-30
EP3080298B1 (en) 2018-10-31
AU2021206868B2 (en) 2023-12-21
EP3495506B1 (en) 2023-07-12
KR20240025725A (ko) 2024-02-27

Similar Documents

Publication Publication Date Title
US12234512B2 (en) Compositions and methods for detecting rare sequence variants
US20230357836A1 (en) Compositions and methods for detecting rare sequence variants
US11859246B2 (en) Methods and compositions for enrichment of amplification products
US11286519B2 (en) Methods and compositions for enrichment of amplification products
US20210301328A1 (en) Compositions and methods for digital polymerase chain reaction
US20250154602A1 (en) Compositions and methods for detecting rare sequence variants
HK40002630A (en) Methods for detecting rare sequence variants
HK40002630B (en) Methods for detecting rare sequence variants
HK1238681B (en) Methods for detecting rare sequence variants
HK1238681A1 (en) Methods for detecting rare sequence variants

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20191209

EEER Examination request

Effective date: 20191209

EEER Examination request

Effective date: 20191209

EEER Examination request

Effective date: 20191209

EEER Examination request

Effective date: 20191209

EEER Examination request

Effective date: 20191209

EEER Examination request

Effective date: 20191209

EEER Examination request

Effective date: 20191209