CA2933343A1 - Compositions and methods for detecting rare sequence variants - Google Patents

Compositions and methods for detecting rare sequence variants Download PDF

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Publication number
CA2933343A1
CA2933343A1 CA2933343A CA2933343A CA2933343A1 CA 2933343 A1 CA2933343 A1 CA 2933343A1 CA 2933343 A CA2933343 A CA 2933343A CA 2933343 A CA2933343 A CA 2933343A CA 2933343 A1 CA2933343 A1 CA 2933343A1
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Prior art keywords
sequence
polynucleotides
primer
amplification
cftr
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CA2933343A
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English (en)
French (fr)
Inventor
Shengrong LIN
Zhaohui Sun
Grace Qizhi ZHAO
Paul Ling-Fung TANG
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Accuragen Holdings Ltd
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Accuragen Inc
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/307—Circular oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
    • C—CHEMISTRY; METALLURGY
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/131—Inverse PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2933343A 2013-12-11 2014-12-11 Compositions and methods for detecting rare sequence variants Pending CA2933343A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361914907P 2013-12-11 2013-12-11
US61/914,907 2013-12-11
US201461987414P 2014-05-01 2014-05-01
US61/987,414 2014-05-01
US201462010975P 2014-06-11 2014-06-11
US62/010,975 2014-06-11
PCT/US2014/069848 WO2015089333A1 (en) 2013-12-11 2014-12-11 Compositions and methods for detecting rare sequence variants

Publications (1)

Publication Number Publication Date
CA2933343A1 true CA2933343A1 (en) 2015-06-18

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2933343A Pending CA2933343A1 (en) 2013-12-11 2014-12-11 Compositions and methods for detecting rare sequence variants

Country Status (12)

Country Link
US (4) US10767222B2 (OSRAM)
EP (2) EP3495506B1 (OSRAM)
JP (1) JP6435334B2 (OSRAM)
KR (3) KR102379877B1 (OSRAM)
CN (2) CN109706222A (OSRAM)
AU (3) AU2014362227B2 (OSRAM)
CA (1) CA2933343A1 (OSRAM)
ES (2) ES2707744T3 (OSRAM)
IL (3) IL300974A (OSRAM)
MX (1) MX376900B (OSRAM)
SG (2) SG11201604737VA (OSRAM)
WO (1) WO2015089333A1 (OSRAM)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017037657A1 (en) * 2015-09-02 2017-03-09 Robert Osborne Method of identifying sequence variants using concatenation
CN110872632A (zh) * 2018-08-30 2020-03-10 深圳华大生命科学研究院 咽颊炎链球菌的特异基因序列、其检测引物及其应用

Families Citing this family (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP1984738A2 (en) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2007133710A2 (en) 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (en) 2007-04-19 2008-10-30 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
WO2012040387A1 (en) 2010-09-24 2012-03-29 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
EP3709018A1 (en) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Microfluidic apparatus for identifying components of a chemical reaction
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US9792405B2 (en) 2013-01-17 2017-10-17 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
US10691775B2 (en) 2013-01-17 2020-06-23 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
US10068054B2 (en) 2013-01-17 2018-09-04 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
US9679104B2 (en) 2013-01-17 2017-06-13 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
US10847251B2 (en) 2013-01-17 2020-11-24 Illumina, Inc. Genomic infrastructure for on-site or cloud-based DNA and RNA processing and analysis
WO2014113736A1 (en) 2013-01-17 2014-07-24 Edico Genome Corp. Bioinformatics systems, apparatuses, and methods executed on an integrated circuit processing platform
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
WO2015089333A1 (en) * 2013-12-11 2015-06-18 Accuragen, Inc. Compositions and methods for detecting rare sequence variants
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US9944977B2 (en) * 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
ES2833299T3 (es) 2014-02-04 2021-06-14 Jumpcode Genomics Inc Fraccionamiento del genoma
PT3152331T (pt) 2014-06-06 2019-11-05 Univ Cornell Método para identificação e enumeração de alterações de sequência de ácidos nucleicos, expressão, cópia ou metilação de adn, utilizando reações de nuclease, ligase, polimerase e sequenciação combinadas
WO2016025818A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
EP3224595A4 (en) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
WO2016053638A1 (en) * 2014-09-30 2016-04-07 Ge Healthcare Bio-Sciences Corp. Method for nucleic acid analysis directly from an unpurified biological sample
EP3329491A2 (en) 2015-03-23 2018-06-06 Edico Genome Corporation Method and system for genomic visualization
IL285795B (en) * 2015-08-12 2022-07-01 Univ Hong Kong Chinese Single-molecule sequencing of plasma dna
EP3347466B9 (en) 2015-09-08 2024-06-26 Cold Spring Harbor Laboratory Genetic copy number determination using high throughput multiplex sequencing of smashed nucleotides
HK1259101A1 (zh) * 2015-10-09 2019-11-22 Accuragen Holdings Limited 用於富集扩增产物的方法及组合物
CA3002560A1 (en) 2015-10-23 2017-04-27 Array Biopharma, Inc. 2-aryl- and 2-heteroaryl-substituted 2-pyridazin-3(2h)-one compounds as inhibitors of fgfr tyrosine kinases
CN108699505A (zh) * 2015-12-03 2018-10-23 安可济控股有限公司 用于形成连接产物的方法和组合物
US20170270245A1 (en) 2016-01-11 2017-09-21 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods for performing secondary and/or tertiary processing
US10068183B1 (en) 2017-02-23 2018-09-04 Edico Genome, Corp. Bioinformatics systems, apparatuses, and methods executed on a quantum processing platform
WO2017136720A1 (en) * 2016-02-05 2017-08-10 Good Start Genetics, Inc. Variant detection of sequencing assays
CN116987777A (zh) 2016-03-25 2023-11-03 凯锐思公司 合成核酸掺入物
WO2017190067A1 (en) * 2016-04-28 2017-11-02 Impact Genomics, Inc. Methods of assessing and monitoring tumor load
US10354747B1 (en) * 2016-05-06 2019-07-16 Verily Life Sciences Llc Deep learning analysis pipeline for next generation sequencing
CA3024630A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
US20190112649A1 (en) 2016-05-31 2019-04-18 Quantapore, Inc. Two-color nanopore sequencing
US11001893B2 (en) 2016-06-10 2021-05-11 Zinfandel Pharmaceuticals, Inc. Methods for detecting structural variants in neurodegenerative disease
WO2017218737A2 (en) * 2016-06-17 2017-12-21 Ludwig Institute For Cancer Research Ltd Methods of small-rna transcriptome sequencing and applications thereof
US20190323073A1 (en) * 2016-06-23 2019-10-24 Accuragen Holdings Limited Cell-free nucleic acid standards and uses thereof
US10144962B2 (en) 2016-06-30 2018-12-04 Grail, Inc. Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library
TWI699435B (zh) * 2016-07-01 2020-07-21 體學生物科技股份有限公司 建構環狀模板和檢測dna分子的方法
JP6876785B2 (ja) * 2016-07-18 2021-05-26 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 単分子配列決定のための一本鎖環状ｄｎａライブラリーを生成するための方法
CN109844132B (zh) * 2016-08-10 2023-11-03 格瑞尔有限责任公司 分析核酸片段的方法
CN106282161B (zh) * 2016-08-12 2020-10-30 成都诺恩基因科技有限公司 特异捕获并重复复制低频率dna碱基变异的方法及应用
EP3497245A4 (en) 2016-08-15 2020-06-03 Accuragen Holdings Limited COMPOSITIONS AND METHODS FOR DETECTING RARE SEQUENCE VARIANTS
JP6810559B2 (ja) * 2016-09-09 2021-01-06 株式会社日立製作所 環状型一本鎖核酸、およびその調製方法と使用方法
US10683531B2 (en) * 2016-09-15 2020-06-16 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free DNA
CA3037185A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
WO2018077847A1 (en) * 2016-10-31 2018-05-03 F. Hoffmann-La Roche Ag Barcoded circular library construction for identification of chimeric products
JP7079029B2 (ja) * 2017-04-17 2022-06-01 ジーンファースト リミテッド 核酸ライブラリーを調製するための方法、組成物、およびキット
CN110603334B (zh) * 2017-06-20 2024-01-16 深圳华大智造科技股份有限公司 Pcr引物对及其应用
CN111051511A (zh) * 2017-08-04 2020-04-21 十亿至一公司 用于与生物靶相关的表征的靶相关分子
IL319255A (en) * 2017-08-07 2025-04-01 Univ Johns Hopkins Methods and materials for cancer assessment and treatment
US10350307B2 (en) * 2017-09-18 2019-07-16 General Electric Company In vivo RNA or protein expression using double-stranded concatemeric DNA including phosphorothioated nucleotides
WO2019060722A2 (en) 2017-09-22 2019-03-28 X Gen Us Co. METHODS AND COMPOSITIONS FOR USE IN PREPARING POLYNUCLEOTIDES
JP7067896B2 (ja) * 2017-10-27 2022-05-16 シスメックス株式会社 品質評価方法、品質評価装置、プログラム、および記録媒体
WO2019086531A1 (en) * 2017-11-03 2019-05-09 F. Hoffmann-La Roche Ag Linear consensus sequencing
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
WO2019191900A1 (en) 2018-04-03 2019-10-10 Burning Rock Biotech Compositions and methods for preparing nucleic acid libraries
WO2019210049A1 (en) 2018-04-27 2019-10-31 X Gen Us Co. Methods and compositions for preparing polynucleotides
CN112534063A (zh) 2018-05-22 2021-03-19 安序源有限公司 用于核酸测序的方法、系统和组合物
WO2019241290A1 (en) 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products
CN112654713A (zh) * 2018-07-05 2021-04-13 安可济控股有限公司 用于数字聚合酶链反应的组合物和方法
SG11202100564PA (en) * 2018-07-23 2021-02-25 Univ Hong Kong Chinese Cell-free dna damage analysis and its clinical applications
EP3884065B1 (en) 2018-11-21 2024-08-14 Karius, Inc. Direct-to-library methods, systems, and compositions
WO2020131923A1 (en) * 2018-12-17 2020-06-25 uBiome, Inc. Automatic standardization pipeline for data analysis
EP3898626A1 (en) 2018-12-19 2021-10-27 Array Biopharma, Inc. Substituted pyrazolo[1,5-a]pyridine compounds as inhibitors of fgfr tyrosine kinases
WO2020131674A1 (en) 2018-12-19 2020-06-25 Array Biopharma Inc. 7-((3,5-dimethoxyphenyl)amino)quinoxaline derivatives as fgfr inhibitors for treating cancer
EP3927838A4 (en) * 2019-02-22 2022-11-16 AccuraGen Holdings Limited METHODS AND COMPOSITIONS FOR THE EARLY DETECTION OF CANCER
WO2020183280A1 (en) * 2019-03-14 2020-09-17 Genome Research Limited Method for sequencing a direct repeat
JP7675700B2 (ja) * 2019-08-21 2025-05-13 ライフ テクノロジーズ コーポレーション シーケンシングのためのシステムおよび方法
CA3154235A1 (en) * 2019-09-13 2021-03-18 University Health Network Detection of circulating tumor dna using double stranded hybrid capture
US11788137B2 (en) 2019-09-30 2023-10-17 Diagenode S.A. Diagnostic and/or sequencing method and kit
EP3828283A1 (en) * 2019-11-28 2021-06-02 Diagenode S.A. An improved sequencing method and kit
EP4061963A1 (en) * 2019-11-19 2022-09-28 Xbf Llc Method for identifying gene fusions by circle cdna amplification
US20240279735A1 (en) * 2019-12-20 2024-08-22 Korea University Research And Business Foundation Primers for detecting trace amount of rare single- nucleotide variant and method for specifically and sensitively detecting trace amount of rare single-nucleotide variant by using same
US12441707B2 (en) 2019-12-30 2025-10-14 Tyra Biosciences, Inc. Indazole compounds
US20230115945A1 (en) 2019-12-30 2023-04-13 Tyra Biosciences, Inc. Aminopyrimidine compounds
AU2020423361B2 (en) * 2020-01-17 2024-08-29 Mgi Tech Co., Ltd. Method for synchronously sequencing sense strand and antisense strand of DNA
CA3182741A1 (en) * 2020-06-15 2021-12-23 Aziz AL'KHAFAJI Chimeric amplicon array sequencing
WO2022018055A1 (en) * 2020-07-20 2022-01-27 Westfälische Wilhelms-Universität Münster Circulation method to sequence immune repertoires of individual cells
EP4208470A4 (en) * 2020-10-22 2024-10-30 Singular Genomics Systems, Inc. NUCLEIC ACID CIRCULARIZATION AND AMPLIFICATION ON A SURFACE
US12264149B2 (en) 2020-12-30 2025-04-01 Tyra Biosciences, Inc. Indazole compounds as kinase inhibitors
US20230187023A1 (en) * 2021-02-03 2023-06-15 Tohoku University Genetic information analysis system and genetic information analysis method
EP4047100A1 (en) * 2021-02-18 2022-08-24 Miltenyi Biotec B.V. & Co. KG Ngs targeted dna panel using multiplex selective rca
MX2023009954A (es) 2021-02-26 2023-11-09 Tyra Biosciences Inc Compuestos de aminopirimidina y métodos de uso de estos.
WO2022204464A1 (en) * 2021-03-26 2022-09-29 Flagship Pioneering Innovations Vii, Llc Production of circular polyribonucleotides in a eukaryotic system
EP4314277A1 (en) * 2021-03-26 2024-02-07 Flagship Pioneering Innovations VII, LLC Compositions and methods for producing circular polyribonucleotides
WO2022204466A1 (en) * 2021-03-26 2022-09-29 Flagship Pioneering Innovations Vii, Llc Production of circular polyribonucleotides in a prokaryotic system
WO2022212559A1 (en) * 2021-03-31 2022-10-06 Jumpcode Genomics, Inc. Methods for targeted nucleic acid sequencing
CN113549674B (zh) * 2021-04-22 2023-11-03 福建和瑞基因科技有限公司 一种检测样本中目标序列整合和突变的方法及其引物的设计方法和试剂盒
CN118318049A (zh) 2021-09-28 2024-07-09 安序源有限公司 用于处理核酸样品的方法以及核酸样品的组合物
CN118715566A (zh) 2022-03-08 2024-09-27 因美纳有限公司 多通道软件加速基因组读段映射引擎
EP4547670A1 (en) 2022-06-29 2025-05-07 Tyra Biosciences, Inc. Polymorphic compounds and uses thereof
AU2023300362A1 (en) 2022-06-29 2025-01-09 Tyra Biosciences, Inc. Indazole compounds
US20240068010A1 (en) * 2022-08-31 2024-02-29 Genomill Health Oy Highly sensitive methods for accurate parallel quantification of variant nucleic acids
WO2024138112A1 (en) 2022-12-22 2024-06-27 Tyra Biosciences, Inc. Indazole compounds
TW202535368A (zh) 2023-12-15 2025-09-16 美商泰拉生物科學公司 吲唑化合物
WO2025132779A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025222097A1 (en) 2024-04-19 2025-10-23 Tyra Biosciences, Inc. Indazole-based protac degraders and their anticancer activity

Family Cites Families (167)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5527676A (en) 1989-03-29 1996-06-18 The Johns Hopkins University Detection of loss of the wild-type P53 gene and kits therefor
EP0390323B2 (en) 1989-03-29 2012-08-08 Johns Hopkins University Detection of loss of the wild-type p53 gene
US5362623A (en) 1991-06-14 1994-11-08 The John Hopkins University Sequence specific DNA binding by p53
US6677312B1 (en) 1989-03-29 2004-01-13 The Johns Hopkins University Methods for restoring wild-type p53 gene function
US6800617B1 (en) 1989-03-29 2004-10-05 The Johns Hopkins University Methods for restoring wild-type p53 gene function
EP0731175A3 (en) 1989-07-11 2004-05-26 Gen-Probe Incorporated Oligonucleotide probes for HIV nucleic acid
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5527670A (en) 1990-09-12 1996-06-18 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5352775A (en) 1991-01-16 1994-10-04 The Johns Hopkins Univ. APC gene and nucleic acid probes derived therefrom
AU1366992A (en) 1991-01-16 1992-08-27 Cancer Institute, The Inherited and somatic mutations of apc gene in colorectal cancer of humans
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5330892A (en) 1991-03-13 1994-07-19 The Johns Hopkins University MCC gene (mutated in colorectal cancer) used for diagnosis of cancer in humans
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5492808A (en) 1993-05-05 1996-02-20 The Johns Hopkins University Means for detecting familial colon cancer (FCC)
US7326778B1 (en) 1993-05-05 2008-02-05 The John Hopkins University Mutator gene and hereditary non-polyposis colorectal cancer
US5591826A (en) 1993-05-05 1997-01-07 The Johns Hopkins University Human MSH2 protein
ATE165118T1 (de) 1993-06-09 1998-05-15 Gamera Bioscience Corp Magnetische zyklusreaktionen
WO1995013375A1 (en) 1993-11-10 1995-05-18 The Johns Hopkins University Tumor suppressor waf1
ATE273387T1 (de) 1993-12-02 2004-08-15 Univ Johns Hopkins Menschliches mutator-gen nmsh2 und seine verbindung zum hereditaren, nichtpolypösen kolorrektalen karzinom
US6482606B1 (en) 1994-01-27 2002-11-19 Human Genome Sciences, Inc. Human DNA mismatch repair polynucleotides
US6380369B1 (en) 1994-01-27 2002-04-30 Human Genome Sciences, Inc. Human DNA mismatch repair proteins
EP0749496A4 (en) 1994-01-27 1999-03-03 Human Genome Sciences Inc HUMAN DNA MALFUNCTION REPAIR PROTEINS
WO1995025177A1 (en) 1994-03-15 1995-09-21 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US6593086B2 (en) 1996-05-20 2003-07-15 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
ATE199572T1 (de) 1995-11-21 2001-03-15 Univ Yale Unimolekulare segmentamplifikation und bestimmung
US6511805B1 (en) 1996-03-15 2003-01-28 The Penn State Research Foundation Methods for detecting papillomavirus DNA in blood plasma and serum
US8048629B2 (en) 1996-03-15 2011-11-01 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
AU2324997A (en) 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
ES2347854T5 (es) 1996-03-26 2014-07-24 Michael S. Kopreski Método que permite el uso de RNA extracelular extraído de plasma o suero para detectar, monitorizar o evaluar un cáncer
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
ATE364718T1 (de) 1997-04-01 2007-07-15 Solexa Ltd Verfahren zur vervielfältigung von nukleinsäure
GB9706654D0 (en) 1997-04-02 1997-05-21 Scient Generics Ltd Disassociation of interacting molecules
WO1998045474A1 (en) 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
WO1999053102A1 (en) 1998-04-16 1999-10-21 Packard Bioscience Company Analysis of polynucleotide sequence
DE69940623D1 (de) 1998-11-09 2009-04-30 Eiken Chemical Prozess zur Synthetisierung von Nukleinsäure
GB9903906D0 (en) 1999-02-19 1999-04-14 Microbiological Res Authority Method and apparatus for nucleic acid strand separation
DE60033647T2 (de) 1999-03-17 2007-11-15 The Board Of Trustees Of The Leland Stanford Junior University, Stanford Detektion von fehlbasenpaarungen
AU4454000A (en) * 1999-04-12 2000-11-14 Genentech Inc. Pcr-based cloning method
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
AU7364700A (en) 1999-09-08 2001-04-10 University Of Victoria Innovation And Development Corporation Use of psychrotrophic bacterium in biotechnology applications
AU783873B2 (en) 1999-09-13 2005-12-15 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
AU783841B2 (en) * 1999-11-26 2005-12-15 454 Life Sciences Corporation Nucleic acid probe arrays
US6653079B2 (en) 2000-03-13 2003-11-25 Freshgene, Inc. Methods for detection of differences in nucleic acids
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US6626906B1 (en) 2000-10-23 2003-09-30 Sdgi Holdings, Inc. Multi-planar adjustable connector
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
JP2004526442A (ja) 2001-02-27 2004-09-02 ビルコ・ビーブイビーエイ エネルギー転移プライマーを用いる環状プローブ増幅（ｃｐａ）
NZ523167A (en) 2001-03-09 2006-08-31 Nugen Technologies Inc Methods, compositions and kits for amplifying RNA sequences of interest which employ an RNA/DNA composite primer
US7122715B2 (en) 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US6815167B2 (en) 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
CA2498764C (en) 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
WO2004044225A2 (en) 2002-11-11 2004-05-27 Affymetrix, Inc. Methods for identifying dna copy number changes
EP1590470A4 (en) 2003-02-03 2006-09-27 Ge Healthcare Bio Sciences CDNA AMPLIFICATION FOR EXPRESSION PROFILING
WO2005059157A2 (en) 2003-12-11 2005-06-30 The Board Of Trustees Of The Leland Stanford Junior University METHODS AND COMPOSITIONS FOR USE IN PREPARING HAIRPIN RNAs
EP2789383B1 (en) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molecular arrays
JP2007526772A (ja) 2004-02-27 2007-09-20 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ インサイチュー配列決定用ポロニー蛍光ビーズ
WO2005095654A1 (en) 2004-03-25 2005-10-13 Biohelix Corporation Helicase-dependent amplification of circular nucleic acids
US8039210B2 (en) 2004-05-14 2011-10-18 The Johns Hopkins University Protein tyrosine phosphatase mutations in cancers
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
EP2319940A1 (en) 2005-01-04 2011-05-11 Hitachi Chemical Company, Ltd. Primer generation rolling circle amplification
EP1869212A4 (en) 2005-02-01 2009-09-23 Wayne John Cancer Inst USE OF ID4 TO DIAGNOSE AND TREAT CANCER
US7824890B2 (en) 2005-02-19 2010-11-02 Avacta Group Plc Isothermal amplification of nucleic acids
DE602005009324D1 (de) 2005-04-06 2008-10-09 Maurice Stroun Methode zur Krebsdiagnose mittels Nachweis von DNA und RNA im Kreislauf
US9139849B2 (en) 2005-04-08 2015-09-22 The United States of America as Represented by the Government of the Department of Health and Human Services Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
JP2008538496A (ja) * 2005-04-12 2008-10-30 ４５４ ライフ サイエンシーズ コーポレイション ウルトラディープ配列決定を用いて配列変異体を決定するための方法
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
CA2611671C (en) 2005-06-15 2013-10-08 Callida Genomics, Inc. Single molecule arrays for genetic and chemical analysis
US8278035B2 (en) 2005-08-19 2012-10-02 Bioventures, Inc. Method and substances for isolating miRNAs
WO2007133831A2 (en) * 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
CA2631248C (en) 2005-11-28 2016-01-12 Pacific Biosciences Of California, Inc. Uniform surfaces for hybrid material substrates and methods for making and using same
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
US20100291548A1 (en) 2006-03-12 2010-11-18 Applera Corporation Methods of Detecting Target Nucleic Acids
EP2021514A2 (en) 2006-05-10 2009-02-11 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US8768629B2 (en) 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
US8679741B2 (en) * 2006-05-31 2014-03-25 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
AU2007203390B2 (en) 2007-04-26 2012-10-04 Yew Jin Fong Improved lace fastener
FR2924440B1 (fr) 2007-12-04 2015-01-09 Pf Medicament Nouveau procede de generation et de criblage d'une banque d'anticorps
JP2012517238A (ja) * 2009-02-11 2012-08-02 カリス エムピーアイ インコーポレイテッド 腫瘍の分子プロファイリング法
DK2396430T3 (da) 2009-02-16 2013-07-15 Epict Technologies Corp Template-uafhængig ligering af enkelt-strenget dna
US8986958B2 (en) 2009-03-30 2015-03-24 Life Technologies Corporation Methods for generating target specific probes for solution based capture
WO2010115100A1 (en) 2009-04-03 2010-10-07 L&C Diagment, Inc. Multiplex nucleic acid detection methods and systems
US8962253B2 (en) * 2009-04-13 2015-02-24 Somagenics Inc. Methods and compositions for detection of small RNAs
GB0910302D0 (en) 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
US20110237444A1 (en) 2009-11-20 2011-09-29 Life Technologies Corporation Methods of mapping genomic methylation patterns
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8801578B2 (en) 2009-12-21 2014-08-12 Core Industries, Llc Instructional displays and methods for exercise machine
RU2518090C2 (ru) * 2009-12-22 2014-06-10 Дзе Проктер Энд Гэмбл Компани Жидкий состав для чистки и/или глубокой очистки
US8889350B2 (en) 2010-03-26 2014-11-18 Swift Biosciences, Inc. Methods and compositions for isolating polynucleotides
CN101935697B (zh) * 2010-04-16 2015-11-25 中生方政生物技术有限公司 用于核酸序列检测的方法和试剂盒
WO2011161549A2 (en) 2010-06-24 2011-12-29 Population Genetics Technologies Ltd. Methods and compositions for polynucleotide library production, immortalization and region of interest extraction
US20120046877A1 (en) 2010-07-06 2012-02-23 Life Technologies Corporation Systems and methods to detect copy number variation
US9518295B2 (en) 2010-08-11 2016-12-13 Institute Of Psychology, Chinese Academy Of Sciences High-throughput sequencing method for methylated DNA and use thereof
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
CN101985654A (zh) 2010-10-21 2011-03-16 徐州医学院 检测多样本多位点snp的检测方法
US9260753B2 (en) * 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
WO2013003585A2 (en) 2011-06-29 2013-01-03 The Johns Hopkins University Enrichment of nucleic acids by complimentary capture
EP2740804A4 (en) 2011-08-05 2015-06-24 Toshiba Kk PROCESS FOR NUCLEIC ACID ANALYSIS
US9586987B2 (en) 2011-09-08 2017-03-07 Kabushiki Kaisha Dnaform Primer set for isothermal amplication of a target nucleic acid sequence
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
WO2013059740A1 (en) 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
WO2013074632A1 (en) 2011-11-14 2013-05-23 Rose Floyd D Mismatch nucleotide purification and identification
US20130217023A1 (en) * 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
DK2828218T3 (da) 2012-03-20 2020-11-02 Univ Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
CA2872141C (en) 2012-05-31 2016-01-19 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
US10538807B2 (en) 2012-06-01 2020-01-21 Fred Hutchinson Cancer Research Center Compositions and methods for detecting rare nucleic acid molecule mutations
CN104350161A (zh) 2012-06-14 2015-02-11 弗莱德哈钦森癌症研究中心 用于核酸分子中的敏感突变检测的组合物和方法
CN107739754A (zh) 2012-07-11 2018-02-27 北京贝瑞和康生物技术有限公司 片断dna检测方法、片断dna检测试剂盒及其应用
EP2875173B1 (en) 2012-07-17 2017-06-28 Counsyl, Inc. System and methods for detecting genetic variation
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
CA2878246C (en) 2012-07-20 2022-01-11 Verinata Health, Inc. Detecting and classifying copy number variation in a cancer genome
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20140066317A1 (en) 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
AU2013337915A1 (en) 2012-10-31 2015-05-21 Becton, Dickinson And Company Selective amplification and real-time PCR detection of rare mutations
EP3939614A1 (en) 2013-01-18 2022-01-19 Foundation Medicine, Inc. Methods of treating cholangiocarcinoma
EP3988671A1 (en) 2013-02-20 2022-04-27 Emory University Compositions for sequencing nucleic acids in mixtures
AU2014233373B2 (en) 2013-03-15 2019-10-24 Verinata Health, Inc. Generating cell-free DNA libraries directly from blood
WO2014145128A2 (en) 2013-03-15 2014-09-18 Arnold Lyle J Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
EP3421613B1 (en) 2013-03-15 2020-08-19 The Board of Trustees of the Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
US20150033372A1 (en) 2013-05-01 2015-01-29 Kymab Limited Human VpreB & Chimaeric Surrogate Light Chains in Transgenic Non-Human Vertebrates
US9644232B2 (en) 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9499870B2 (en) 2013-09-27 2016-11-22 Natera, Inc. Cell free DNA diagnostic testing standards
US10941397B2 (en) 2013-10-17 2021-03-09 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
EP4130350A1 (en) 2013-11-07 2023-02-08 The Board of Trustees of the Leland Stanford Junior University Cell-free nucleic acids for the analysis of the human microbiome and components thereof
GB201321123D0 (en) 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
WO2015089333A1 (en) 2013-12-11 2015-06-18 Accuragen, Inc. Compositions and methods for detecting rare sequence variants
SG10201804519RA (en) 2013-12-28 2018-07-30 Guardant Health Inc Methods and systems for detecting genetic variants
CN104745679B (zh) 2013-12-31 2018-06-15 杭州贝瑞和康基因诊断技术有限公司 一种无创检测egfr基因突变的方法及试剂盒
KR102482592B1 (ko) 2014-01-31 2022-12-30 인티그레이티드 디엔에이 테크놀로지스 아이엔씨. Dna 기질을 처리하는 개선 방법
PT3152331T (pt) 2014-06-06 2019-11-05 Univ Cornell Método para identificação e enumeração de alterações de sequência de ácidos nucleicos, expressão, cópia ou metilação de adn, utilizando reações de nuclease, ligase, polimerase e sequenciação combinadas
CA3172086A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
EP3169793B1 (en) 2014-07-16 2022-07-06 Tangen Biosciences Inc. Isothermal methods for amplifying nucleic acid samples
WO2016053638A1 (en) 2014-09-30 2016-04-07 Ge Healthcare Bio-Sciences Corp. Method for nucleic acid analysis directly from an unpurified biological sample
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
HK1259101A1 (zh) 2015-10-09 2019-11-22 Accuragen Holdings Limited 用於富集扩增产物的方法及组合物
CN108699505A (zh) 2015-12-03 2018-10-23 安可济控股有限公司 用于形成连接产物的方法和组合物
CA3024630A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
US10370701B2 (en) 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
US20190323073A1 (en) 2016-06-23 2019-10-24 Accuragen Holdings Limited Cell-free nucleic acid standards and uses thereof
EP3497245A4 (en) 2016-08-15 2020-06-03 Accuragen Holdings Limited COMPOSITIONS AND METHODS FOR DETECTING RARE SEQUENCE VARIANTS
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017037657A1 (en) * 2015-09-02 2017-03-09 Robert Osborne Method of identifying sequence variants using concatenation
US10519488B2 (en) 2015-09-02 2019-12-31 Oxford Nanopore Technologies, Ltd. Method of identifying sequence variants using concatenation
US11111524B2 (en) 2015-09-02 2021-09-07 Oxford Nanopore Technologies, Ltd. Method of identifying sequence variants using concatenation
US12104202B2 (en) 2015-09-02 2024-10-01 Oxford Nanopore Technologies Plc Method of identifying sequence variants using concatenation
CN110872632A (zh) * 2018-08-30 2020-03-10 深圳华大生命科学研究院 咽颊炎链球菌的特异基因序列、其检测引物及其应用

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US10767222B2 (en) 2020-09-08
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ES2707744T3 (es) 2019-04-04
CN104946737A (zh) 2015-09-30
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