SG10201807532SA - Compositions and methods for detecting rare sequence variants - Google Patents

Compositions and methods for detecting rare sequence variants

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Publication number
SG10201807532SA
SG10201807532SA SG10201807532SA SG10201807532SA SG10201807532SA SG 10201807532S A SG10201807532S A SG 10201807532SA SG 10201807532S A SG10201807532S A SG 10201807532SA SG 10201807532S A SG10201807532S A SG 10201807532SA SG 10201807532S A SG10201807532S A SG 10201807532SA
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Singapore
Prior art keywords
methods
compositions
sequence
sequence variants
detecting rare
Prior art date
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SG10201807532SA
Inventor
Shengrong Lin
Zhaohui Sun
Grace Qizhi Zhao
Paul Ling-Fung Tang
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Accuragen Holdings Ltd
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Application filed by Accuragen Holdings Ltd filed Critical Accuragen Holdings Ltd
Publication of SG10201807532SA publication Critical patent/SG10201807532SA/en

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/307—Circular oligonucleotides
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
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    • C12Q2531/00—Reactions of nucleic acids characterised by
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Abstract

Compositions and methods for detecting rare sequence variants In some aspects, the present disclosure provides methods for identifying sequence variants in a nucleic acid sample. In some embodiments, a method comprises identifying sequence differences between sequencing reads and a reference sequence, and calling a sequence difference that occurs in at least two different circular polynucleotides, such as two circular polynucleotides having different junctions, as the sequence variant. In some aspects, the present disclosure provides compositions and systems useful in the described methods. Fig. 7
SG10201807532SA 2013-12-11 2014-12-11 Compositions and methods for detecting rare sequence variants SG10201807532SA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361914907P 2013-12-11 2013-12-11
US201461987414P 2014-05-01 2014-05-01
US201462010975P 2014-06-11 2014-06-11

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SG10201807532SA true SG10201807532SA (en) 2018-09-27

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SG11201604737VA SG11201604737VA (en) 2013-12-11 2014-12-11 Compositions and methods for detecting rare sequence variants

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US (3) US10767222B2 (en)
EP (2) EP3495506B1 (en)
JP (1) JP6435334B2 (en)
KR (3) KR20240025725A (en)
CN (2) CN104946737B (en)
AU (3) AU2014362227B2 (en)
CA (1) CA2933343A1 (en)
ES (2) ES2960338T3 (en)
HK (1) HK1214843A1 (en)
IL (3) IL300974A (en)
MX (1) MX2016007605A (en)
SG (2) SG10201807532SA (en)
WO (1) WO2015089333A1 (en)

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