CA2926124C - Methods for forming lipid bilayers on biochips - Google Patents
Methods for forming lipid bilayers on biochips Download PDFInfo
- Publication number
- CA2926124C CA2926124C CA2926124A CA2926124A CA2926124C CA 2926124 C CA2926124 C CA 2926124C CA 2926124 A CA2926124 A CA 2926124A CA 2926124 A CA2926124 A CA 2926124A CA 2926124 C CA2926124 C CA 2926124C
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- Prior art keywords
- lipid
- bubble
- chip
- bilayer
- nanopore
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Nanotechnology (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Hematology (AREA)
- Sustainable Development (AREA)
- Dispersion Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361894661P | 2013-10-23 | 2013-10-23 | |
| US61/894,661 | 2013-10-23 | ||
| PCT/US2014/061853 WO2015061510A1 (en) | 2013-10-23 | 2014-10-23 | Methods for forming lipid bilayers on biochips |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2926124A1 CA2926124A1 (en) | 2015-04-30 |
| CA2926124C true CA2926124C (en) | 2024-04-09 |
Family
ID=52993530
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2926124A Active CA2926124C (en) | 2013-10-23 | 2014-10-23 | Methods for forming lipid bilayers on biochips |
| CA2926140A Active CA2926140C (en) | 2013-10-23 | 2014-10-23 | Process for biosensor well formation |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2926140A Active CA2926140C (en) | 2013-10-23 | 2014-10-23 | Process for biosensor well formation |
Country Status (7)
| Country | Link |
|---|---|
| US (7) | US9567630B2 (https=) |
| EP (3) | EP3060646B1 (https=) |
| JP (2) | JP6514692B2 (https=) |
| CN (4) | CN105637081B (https=) |
| CA (2) | CA2926124C (https=) |
| ES (1) | ES2779073T3 (https=) |
| WO (2) | WO2015061511A1 (https=) |
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| US10908143B2 (en) * | 2013-11-27 | 2021-02-02 | Hitachi, Ltd. | Current measuring device, current measuring method, and current measuring kit |
| WO2016004029A1 (en) * | 2014-06-30 | 2016-01-07 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | Systems and methods of preparing stabilized lipid assemblies |
| CN104792845A (zh) * | 2014-08-07 | 2015-07-22 | 中国科学院微电子研究所 | 传感装置 |
| CA2966246C (en) | 2014-10-31 | 2020-07-14 | Genia Technologies, Inc. | Alpha-hemolysin variants with altered characteristics |
| USD815752S1 (en) * | 2014-11-28 | 2018-04-17 | Randox Laboratories Ltd. | Biochip well |
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| DE102015106032B4 (de) | 2015-04-20 | 2018-10-31 | Gabriel Krajewski | Vorrichtung und Verfahren zur manuellen Handhabung einer Palette |
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