CA2919833A1 - Quantitative dna-based imaging and super-resolution imaging - Google Patents

Quantitative dna-based imaging and super-resolution imaging Download PDF

Info

Publication number
CA2919833A1
CA2919833A1 CA2919833A CA2919833A CA2919833A1 CA 2919833 A1 CA2919833 A1 CA 2919833A1 CA 2919833 A CA2919833 A CA 2919833A CA 2919833 A CA2919833 A CA 2919833A CA 2919833 A1 CA2919833 A1 CA 2919833A1
Authority
CA
Canada
Prior art keywords
strand
drift
protein
labeled imager
docking
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2919833A
Other languages
English (en)
French (fr)
Inventor
Ralf Jungmann
Peng Yin
Mingjie DAI
Maier AVENDANO
Johannes B. Woehrstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52432405&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2919833(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Harvard University filed Critical Harvard University
Publication of CA2919833A1 publication Critical patent/CA2919833A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/20—Analysis of motion
    • G06T7/246—Analysis of motion using feature-based methods, e.g. the tracking of corners or segments
    • G06T7/248—Analysis of motion using feature-based methods, e.g. the tracking of corners or segments involving reference images or patches
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10—Signal processing, e.g. from mass spectrometry [MS] or from PCR
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B45/00—ICT specially adapted for bioinformatics-related data visualisation, e.g. displaying of maps or networks
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/36—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Actinomyces; from Streptomyces (G)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medical Informatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Data Mining & Analysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Software Systems (AREA)
  • Artificial Intelligence (AREA)
  • Databases & Information Systems (AREA)
  • Evolutionary Computation (AREA)
  • Bioethics (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
CA2919833A 2013-07-30 2014-07-30 Quantitative dna-based imaging and super-resolution imaging Abandoned CA2919833A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361859891P 2013-07-30 2013-07-30
US61/859,891 2013-07-30
US201361884126P 2013-09-29 2013-09-29
US61/884,126 2013-09-29
US201461934759P 2014-02-01 2014-02-01
US61/934,759 2014-02-01
PCT/US2014/048977 WO2015017586A1 (en) 2013-07-30 2014-07-30 Quantitative dna-based imaging and super-resolution imaging

Publications (1)

Publication Number Publication Date
CA2919833A1 true CA2919833A1 (en) 2015-02-05

Family

ID=52432405

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2919833A Abandoned CA2919833A1 (en) 2013-07-30 2014-07-30 Quantitative dna-based imaging and super-resolution imaging

Country Status (9)

Country Link
US (2) US20160161472A1 (cg-RX-API-DMAC7.html)
EP (2) EP3027773B1 (cg-RX-API-DMAC7.html)
JP (1) JP6475718B2 (cg-RX-API-DMAC7.html)
KR (1) KR20160039229A (cg-RX-API-DMAC7.html)
CN (1) CN105531377A (cg-RX-API-DMAC7.html)
AU (1) AU2014296253A1 (cg-RX-API-DMAC7.html)
CA (1) CA2919833A1 (cg-RX-API-DMAC7.html)
HK (1) HK1221747A1 (cg-RX-API-DMAC7.html)
WO (1) WO2015017586A1 (cg-RX-API-DMAC7.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109891218A (zh) * 2016-06-02 2019-06-14 乌尔蒂维尤股份有限公司 促进基于抗体的交换成像的组合物和方法

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP4219750A3 (en) 2013-03-11 2023-12-13 Meso Scale Technologies, LLC. Improved methods for conducting multiplexed assays
KR20160039229A (ko) 2013-07-30 2016-04-08 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 정량적 dna-기반 영상화 및 초해상도 영상화
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
ES2968004T3 (es) 2014-07-30 2024-05-06 Harvard College Construcción de bibliotecas de sondas
CN107924473B (zh) * 2015-08-07 2021-11-23 哈佛学院院长及董事 蛋白质-蛋白质相互作用的超分辨成像
EP3347400A1 (en) 2015-09-10 2018-07-18 King Abdullah University Of Science And Technology Conjugated polymer nanoparticles, methods of using, and methods of making
WO2017049136A1 (en) * 2015-09-18 2017-03-23 President And Fellows Of Harvard College Nucleic acid frameworks for structural determination
WO2017087823A1 (en) 2015-11-18 2017-05-26 Mir Kalim U Super-resolution sequencing
DE102015016353A1 (de) 2015-12-16 2017-06-22 Horst Wochnowski PALM-Verfahren mit mehreren sich nur marginal voneinander unterscheidenden Fluorophoren
EP3414327B1 (en) * 2016-02-10 2020-09-30 The Regents of The University of Michigan Detection of nucleic acids
US20190120767A1 (en) * 2016-04-26 2019-04-25 Ultivue, Inc. Super-Resolution Fluorescence Microscopy Method Using Improved Drift Compensation Markers
EP3448886A4 (en) * 2016-04-26 2019-12-11 Ultivue, Inc. SUPER-HIGH-RESOLUTION IMMUNOFLUORESCENCE WITH BINDING-LIMITED PREVIEW
WO2017200870A1 (en) * 2016-05-15 2017-11-23 Ultivue, Inc. Multiplexed imaging using strand displacement
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CN109789228B (zh) 2016-07-27 2022-10-21 斯坦福大学托管董事会 高度复用荧光成像
EP3290983A1 (de) * 2016-09-05 2018-03-07 Abberior Instruments GmbH Verfahren zum justieren eines laserscanning-fluoreszenzmikroskops und laserscanning-fluoreszenzmikroskop mit einer automatischen justiervorrichtung
EP4339956A3 (en) * 2016-11-08 2024-06-19 President and Fellows of Harvard College Multiplexed imaging using merfish, expansion microscopy, and related technologies
US20180164308A1 (en) 2016-12-09 2018-06-14 Ultivue, Inc. Methods for multiplex imaging using labeled nucleic acid imaging agents
CN118086461A (zh) 2016-12-16 2024-05-28 艾瑞特姆有限责任公司 使用连接扩增的分子检测
WO2018140661A1 (en) * 2017-01-26 2018-08-02 Azure Biosystems, Inc. Devices and methods for imaging biomolecules
DE102017104736B9 (de) * 2017-03-07 2020-06-25 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Verfahren und Vorrichtung zum räumlichen Messen nanoskaliger Strukturen
JP7145517B2 (ja) * 2017-03-08 2022-10-03 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン 分析物の検出
WO2018170518A1 (en) * 2017-03-17 2018-09-20 Apton Biosystems, Inc. Sequencing and high resolution imaging
CN117551741A (zh) 2017-03-31 2024-02-13 乌尔蒂维尤股份有限公司 Dna-抗原交换和扩增
US11788123B2 (en) 2017-05-26 2023-10-17 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
AU2018281745B2 (en) 2017-06-05 2022-05-19 Becton, Dickinson And Company Sample indexing for single cells
US11331019B2 (en) 2017-08-07 2022-05-17 The Research Foundation For The State University Of New York Nanoparticle sensor having a nanofibrous membrane scaffold
CN107894411B (zh) * 2017-10-27 2020-06-02 上海纳米技术及应用国家工程研究中心有限公司 荧光精确定量dna折纸结构完整度的方法
GB2569283B (en) * 2017-12-05 2021-10-13 Continental Automotive Romania Srl Method and device for adaptively enhancing an output of a detector
DK3498865T3 (da) * 2017-12-14 2020-12-21 Univ Muenchen Ludwig Maximilians Påvisning og kvantificering af enkeltmolekyler ved hjælp af DNA-nanoteknologi i microwells
CN107934531B (zh) * 2017-12-25 2024-04-16 福建上源生物科学技术有限公司 一种自动线虫挑选装置
KR20190108023A (ko) * 2018-03-13 2019-09-23 서울대학교산학협력단 초고감도 바이오마커 다중 검출 방법
CN118864552A (zh) * 2018-04-10 2024-10-29 深圳华大智造科技股份有限公司 荧光图像配准方法、基因测序系统与基因测序仪
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
CN109030434B (zh) * 2018-06-20 2021-08-27 天津大学 一种基于Android平台的真菌毒素含量检测方法
US20210223254A1 (en) * 2018-07-09 2021-07-22 Ultivue, Inc. Methods for Multicolor Multiplex Imaging
EP4242324B1 (en) * 2018-08-17 2025-09-24 Becton, Dickinson and Company Aptamer barcoding
US20230176064A1 (en) * 2018-08-28 2023-06-08 Jl Medilabs, Inc. Methods and kits for detecting target substances
CN109255427A (zh) * 2018-08-30 2019-01-22 无锡城市职业技术学院 一种基于量子粒子群优化算法的分子对接计算方法
CN109371031A (zh) * 2018-11-23 2019-02-22 北京化工大学 一种特异性结合牛血清白蛋白核酸适配体的筛选方法
EP3894583A4 (en) 2018-12-13 2022-11-09 President and Fellows of Harvard College AMPLIFICATION METHODS AND SYSTEMS FOR MERFISH AND OTHER APPLICATIONS
EP3670666A1 (en) 2018-12-20 2020-06-24 Freie Universität Berlin Method for optical imaging a target molecule in a sample, a protein-oligonuleotide conjugate for use in the method and a kit for carrying out the method
CN110364224B (zh) * 2018-12-29 2021-06-08 上海北昂医药科技股份有限公司 一种染色体分裂相定位排序方法
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
WO2020190061A1 (ko) * 2019-03-19 2020-09-24 한국생명공학연구원 전사 시스템 기반 고감도 센서
WO2020223368A1 (en) 2019-04-29 2020-11-05 Nautilus Biotechnology, Inc. Methods and systems for integrated on-chip single-molecule detection
CN110838134B (zh) * 2019-10-10 2020-09-29 北京海益同展信息科技有限公司 目标对象的统计方法、装置、计算机设备和存储介质
CN110746286B (zh) * 2019-11-04 2023-04-07 北京维德维康生物技术有限公司 一种丁香酚半抗原、人工抗原及其制备方法与应用
CN115335520A (zh) 2020-01-29 2022-11-11 贝克顿迪金森公司 用于通过测序对单细胞进行空间映射的条形码化的孔
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN111830014B (zh) * 2020-08-04 2023-06-30 济南大学 一种基于聚苯胺吸附双链dna的传感器检测dna的方法
AU2021378290B2 (en) * 2020-11-11 2025-10-30 Nautilus Subsidiary, Inc. Affinity reagents having enhanced binding and detection characteristics
DE102020131047B4 (de) * 2020-11-24 2024-08-22 Abberior Instruments Gmbh Verfahren und Vorrichtung zur Aufnahme nanoskopischer Bilder mehrfach gefärbter Proben
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
KR102573998B1 (ko) 2020-12-30 2023-09-04 주식회사 스타노스 위양성 신호가 제거된 항원 검출방법 및 키트
US20220252514A1 (en) * 2021-02-10 2022-08-11 Star Voltaic, LLC Fluorescent solid-state materials for optical calibration and methods thereof
CN113004885B (zh) * 2021-03-08 2024-06-11 光华临港工程应用技术研发(上海)有限公司 一种用于膨胀超分辨成像的dna纳米结构染料及其应用
EP4092414B1 (en) * 2021-05-19 2025-01-29 Leica Microsystems CMS GmbH Connector, marker and method for analysing biological samples
CN113281320B (zh) * 2021-06-07 2022-04-29 江南大学 一种基于荧光铜纳米粒子检测黄曲霉毒素b1的方法
EP4662234A2 (en) * 2023-02-08 2025-12-17 Toreador Therapeutics, Inc. Super-resolution microscopy (srm) multiplexing with cleavable moieties
GB202304755D0 (en) * 2023-03-30 2023-05-17 Univ Ramot Method of imaging biomolecules
CN116165187B (zh) * 2023-04-23 2023-08-29 深圳大学 一种基于基准标记物的漂移矫正与筛选方法及成像方法
CN116794290B (zh) * 2023-07-03 2025-04-08 上海市第十人民医院 一种基于可逆dna自组装的组织切片靶标蛋白多重分析方法
EP4488682A1 (en) * 2023-07-06 2025-01-08 Leica Microsystems CMS GmbH Method and system for analysing a biological sample
CN119595679B (zh) * 2024-12-16 2025-10-17 四川大学 一种植鞣皮革文物的鉴定方法

Family Cites Families (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
US5681943A (en) 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
US7569341B2 (en) * 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
DE4421891C2 (de) 1994-06-23 1996-05-15 Helmut Prof Dr Med Feucht Verfahren zum Nachweis von komplementaktivierenden IgG-Antikörpern gegen HLA-DR-Antigene
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
DE19709348C2 (de) 1996-05-29 1999-07-01 Schubert Walter Dr Md Automatisches Multi-Epitop-Ligand-Kartierungsverfahren
US6146828A (en) 1996-08-14 2000-11-14 Exact Laboratories, Inc. Methods for detecting differences in RNA expression levels and uses therefor
US6117631A (en) * 1996-10-29 2000-09-12 Polyprobe, Inc. Detection of antigens via oligonucleotide antibody conjugates
GB9622665D0 (en) * 1996-10-31 1997-01-08 Zeneca Ltd Methods
DE19717904A1 (de) 1997-04-23 1998-10-29 Diagnostikforschung Inst Säurelabile und enzymatisch spaltbare Farbstoffkonstrukte zur Diagnostik mit Nahinfrarotlicht und zur Therapie
US7101672B2 (en) 1998-05-05 2006-09-05 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US5888778A (en) 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
US20100081134A1 (en) 1997-07-21 2010-04-01 Mirkin Chad A Bio-barcode based detection of target analytes
US6083486A (en) 1998-05-14 2000-07-04 The General Hospital Corporation Intramolecularly-quenched near infrared fluorescent probes
US7399844B2 (en) * 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
AU4976699A (en) 1998-07-10 2000-02-01 Chromagen, Inc. Novel fluorogenic substrates for hydrolytic enzymes
US6573043B1 (en) 1998-10-07 2003-06-03 Genentech, Inc. Tissue analysis and kits therefor
US6468785B1 (en) 1999-02-19 2002-10-22 New Mexico State University Technology Transfer Corporation Doped conducting polymers applications and methods
AU3567900A (en) 1999-03-30 2000-10-16 Solexa Ltd. Polynucleotide sequencing
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
DE10014685B4 (de) 2000-03-24 2004-07-01 Schubert, Walter, Dr. Verfahren zur Identifizierung von zellspezifischen Zielstrukturen
US20020142310A1 (en) 2000-04-07 2002-10-03 Leif Andersson Variants of the human AMP-activated protein kinase gamma 3 subunit
AU2001259586A1 (en) 2000-05-05 2001-11-20 Agilix Corporation Highly multiplexed reporter carrier systems
US6689338B2 (en) * 2000-06-01 2004-02-10 The Board Of Regents For Oklahoma State University Bioconjugates of nanoparticles as radiopharmaceuticals
US6511809B2 (en) * 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US6451588B1 (en) 2000-06-30 2002-09-17 Pe Corporation (Ny) Multipartite high-affinity nucleic acid probes
WO2002023156A1 (en) 2000-09-15 2002-03-21 Biogenex Laboratories Enhancement of in situ hybridization
EP1356104A2 (en) 2000-10-06 2003-10-29 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
US20030044353A1 (en) 2001-01-05 2003-03-06 Ralph Weissleder Activatable imaging probes
US20050230255A1 (en) 2001-03-30 2005-10-20 Sumner Lloyd W Silver destaining method
US7219016B2 (en) 2001-04-20 2007-05-15 Yale University Systems and methods for automated analysis of cells and tissues
US20020173053A1 (en) 2001-04-27 2002-11-21 Bassam Damaj Multiple simultaneous antigen detection by immunohistochemistry
US7473767B2 (en) * 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US20040241688A1 (en) 2001-07-19 2004-12-02 Cuneyt Bukusoglu Human tissue specific drug screening procedure
JP2005504281A (ja) 2001-09-24 2005-02-10 ワン ラムダ 診断用プローブ検出システム
US8323903B2 (en) 2001-10-12 2012-12-04 Life Technologies Corporation Antibody complexes and methods for immunolabeling
WO2003061711A2 (en) 2002-01-16 2003-07-31 Visen Medical, Inc. Chromophore probes for optical imaging
US20040018495A1 (en) 2002-07-24 2004-01-29 Xing-Xiang Li Microparticle based signal amplification for the detection of analytes
US7122319B2 (en) 2002-12-18 2006-10-17 Monogram Biosciences, Inc Multiplexed immunohistochemical assays using molecular tags
US7932060B2 (en) 2003-04-18 2011-04-26 Becton, Dickinson And Company Immuno-amplification
WO2005017485A2 (en) 2003-05-22 2005-02-24 Agdia, Inc. Multiplex enzyme-linked immunosorbent assay for detecting multiple analytes
US7279280B2 (en) 2003-09-25 2007-10-09 Mgp Biotech, Inc. Apparatus and method for detecting genetic mutations and single nucleotide polymorphisms
US20050095595A1 (en) 2003-10-29 2005-05-05 Pittaro Richard J. Methods and systems for improving of polymer analysis
EP1692266A4 (en) * 2003-11-06 2009-11-18 Univ Nevada Reno IMPROVED METHODS FOR DETECTING AND MEASURING SPECIFIC NUCLEIC ACID SEQUENCES
US20050287578A1 (en) 2004-06-28 2005-12-29 Exagen Diagnostics, Inc. Methods for RNA fluorescence in situ hybridization
US8153363B2 (en) 2005-01-13 2012-04-10 Progenika Biopharma S.A. Methods and products for in vitro genotyping
US7727721B2 (en) * 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US20060204999A1 (en) 2005-03-14 2006-09-14 Stephen Macevicz Detecting molecular complexes
US7795009B2 (en) 2005-06-15 2010-09-14 Saint Louis University Three-component biosensors for detecting macromolecules and other analytes
US8956857B2 (en) 2005-06-06 2015-02-17 Mediomics, Llc Three-component biosensors for detecting macromolecules and other analytes
US20070048759A1 (en) 2005-06-10 2007-03-01 Dan Luo Detection of target molecules with labeled nucleic acid detection molecules
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
US7494776B2 (en) 2005-07-07 2009-02-24 Beckman Coulter, Inc. Labeled complementary oligonucleotides to detect oligonucleotide-linked ligands
US20070259345A1 (en) 2006-05-03 2007-11-08 Agilent Technologies, Inc. Target determination using compound probes
US20080004185A1 (en) * 2006-06-12 2008-01-03 Antara Biosciences Inc. Three dimensional array
US7838302B2 (en) * 2006-08-07 2010-11-23 President And Fellows Of Harvard College Sub-diffraction limit image resolution and other imaging techniques
US7741045B2 (en) 2006-11-16 2010-06-22 General Electric Company Sequential analysis of biological samples
US20080242560A1 (en) 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
WO2008144562A1 (en) 2007-05-16 2008-11-27 California Institute Of Technology A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
EP2216338B1 (en) 2007-11-19 2013-03-20 Japan Advanced Institute of Science and Technology Light-responsive artificial nucleotide having photo-crosslinking ability
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
EP2350657A4 (en) 2008-11-21 2013-06-12 Univ Saint Louis BIOSENSOR FOR DETECTING MULTIPLE EPITOPES ON ONE TARGET
DE102008062372B3 (de) * 2008-12-17 2010-06-17 Medizinische Hochschule Hannover Nachweiskonjugat und Verfahren zur Analyse
US8785615B2 (en) * 2009-03-25 2014-07-22 Georgia Tech Research Corporation Single molecule sensitive probes for detecting RNA
US9677125B2 (en) 2009-10-21 2017-06-13 General Electric Company Detection of plurality of targets in biological samples
WO2011056864A1 (en) 2009-11-05 2011-05-12 Anaptysbio, Inc. Methods of generating improved antigen-binding agents using chain shuffling and optionally somatic hypermutation
WO2012003330A2 (en) 2010-06-30 2012-01-05 Stratos Genomics, Inc Multiplexed identification of nucleic acid sequences
US20120004132A1 (en) 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
EP3644058A1 (en) 2010-10-14 2020-04-29 Meso Scale Technologies, LLC Reagent storage in an assay device
WO2012058638A2 (en) * 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
EP2643480A4 (en) * 2010-11-22 2014-05-14 Solulink Inc METHOD AND / OR USE OF OLIGONUCLEOTIDE CONJUGATES FOR ASSAYS AND PROOF
US20120178081A1 (en) 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
US8946389B2 (en) * 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
JP5989109B2 (ja) * 2011-07-13 2016-09-07 ライカ バイオシステムズ イメージング インコーポレイテッド 蛍光顕微鏡システムの標準化
EP2737356A1 (en) * 2011-07-25 2014-06-04 Mad City Labs, Inc. Active-feedback positional drift correction in a microscope image using a fiduciary element held on a nanopositioning stage
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
US10150988B2 (en) 2012-08-13 2018-12-11 William Marsh Rice University Multiplexed in situ molecular analyses and programmable molecular probes for regulated single amplification
US10584381B2 (en) * 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014074597A1 (en) 2012-11-06 2014-05-15 President And Fellows Of Harvard College Compositions and methods relating to complex nucleic acid nanostructures
US20160369321A1 (en) 2012-11-14 2016-12-22 Olink Ab RCA Reporter Probes and Their Use in Detecting Nucleic Acid Molecules
EP4219750A3 (en) 2013-03-11 2023-12-13 Meso Scale Technologies, LLC. Improved methods for conducting multiplexed assays
US8933261B2 (en) * 2013-03-14 2015-01-13 Andrei POLUKHTIN Benzophenone-based chromophoric crosslinkers and reagents for incorporation of biotin or other haptens into macromolecules
WO2014145620A2 (en) 2013-03-15 2014-09-18 The Broad Institute, Inc. Novel hybridization probes and uses thereof
KR20160039229A (ko) 2013-07-30 2016-04-08 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 정량적 dna-기반 영상화 및 초해상도 영상화
WO2015070037A2 (en) 2013-11-08 2015-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
WO2015089506A2 (en) 2013-12-15 2015-06-18 President And Fellows Of Harvard College Methods and compositions relating to optical super-resolution patterning
US20170015698A1 (en) 2014-03-08 2017-01-19 President And Fellows Of Harvard College Nucleic acid polyhedra from self-assembled vertex-containing fixed-angle nucleic acid structures
BR112016018314A2 (pt) 2014-03-11 2017-10-17 Harvard College formação de imagem de alto desempenho e altamente multiplexada com sondas programáveis de ácido nucleico
US20160033411A1 (en) 2014-07-29 2016-02-04 President And Fellows Of Harvard College Methods and compositions relating to storm-based patterning
EP3218511B1 (en) 2014-11-11 2020-04-22 Illumina Cambridge Limited Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
US10006917B2 (en) 2014-12-15 2018-06-26 President And Fellows Of Harvard College Methods and compositions relating to super-resolution imaging and modification
CN107924473B (zh) 2015-08-07 2021-11-23 哈佛学院院长及董事 蛋白质-蛋白质相互作用的超分辨成像
US20230176064A1 (en) 2018-08-28 2023-06-08 Jl Medilabs, Inc. Methods and kits for detecting target substances

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109891218A (zh) * 2016-06-02 2019-06-14 乌尔蒂维尤股份有限公司 促进基于抗体的交换成像的组合物和方法

Also Published As

Publication number Publication date
EP3696277B2 (en) 2025-09-03
US20200064340A1 (en) 2020-02-27
AU2014296253A1 (en) 2016-02-04
US11536715B2 (en) 2022-12-27
KR20160039229A (ko) 2016-04-08
EP3696277A2 (en) 2020-08-19
EP3696277B1 (en) 2023-01-25
EP3027773B1 (en) 2020-03-25
EP3027773A4 (en) 2017-03-01
JP2016532689A (ja) 2016-10-20
CN105531377A (zh) 2016-04-27
EP3027773A1 (en) 2016-06-08
HK1221747A1 (zh) 2017-06-09
JP6475718B2 (ja) 2019-02-27
WO2015017586A1 (en) 2015-02-05
US20160161472A1 (en) 2016-06-09
EP3696277A3 (en) 2020-10-21

Similar Documents

Publication Publication Date Title
US11536715B2 (en) Quantitative DNA-based imaging and super-resolution imaging
JP7520172B2 (ja) プログラム可能な核酸プローブを用いた高スループット及び高度多重化イメージング
HK1227106A1 (en) High-throughput and highly multiplexed imaging with programmable nucleic acid probes
HK1227106B (zh) 利用可编程核酸探针的高通量且高度多路复用的成像

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20190716

FZDE Discontinued

Effective date: 20211109