CA2917430C - Methods and compositions for detecting bacterial contamination - Google Patents
Methods and compositions for detecting bacterial contamination Download PDFInfo
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- CA2917430C CA2917430C CA2917430A CA2917430A CA2917430C CA 2917430 C CA2917430 C CA 2917430C CA 2917430 A CA2917430 A CA 2917430A CA 2917430 A CA2917430 A CA 2917430A CA 2917430 C CA2917430 C CA 2917430C
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| EP3543362B1 (en) * | 2013-10-07 | 2021-09-08 | Mitsui Chemicals, Inc. | Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species |
| US9953415B2 (en) * | 2014-12-03 | 2018-04-24 | Tata Consultancy Services Limited | System and method for quantification of Escherichia coli bacteria in water |
| EP3334834B1 (en) * | 2015-08-12 | 2020-05-13 | Circulogene Theranostics, LLC | Method of preparing cell free nucleic acid molecules by in situ amplification |
| CN105368961A (zh) * | 2015-12-15 | 2016-03-02 | 天津脉络生物科技有限公司 | 一种沙雷菌定量检测荧光pcr试剂盒及其应用 |
| CN105734164B (zh) * | 2016-05-06 | 2019-05-17 | 苏州大学 | 一种检测细菌性脑膜炎病原体的多重pcr试剂盒 |
| CN111073987B (zh) * | 2016-08-30 | 2022-09-20 | 上海市生物医药技术研究院 | 小肠结肠炎耶尔森氏菌的快速恒温检测方法、引物组及试剂盒 |
| US20190062809A1 (en) * | 2017-08-24 | 2019-02-28 | Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) | Electrochemical detection of bacterial and/or fungal infections |
| WO2019090306A1 (en) * | 2017-11-06 | 2019-05-09 | The Regents Of The University Of Michigan | Compositions and methods for detection of microorganisms |
| WO2019094973A1 (en) * | 2017-11-13 | 2019-05-16 | Life Technologies Corporation | Compositions, methods and kits for urinary tract microorganism detection |
| CN112105747A (zh) * | 2018-01-30 | 2020-12-18 | 拜奥法尔防护有限责任公司 | 用于验证核酸扩增测定的方法和系统 |
| CN108148834B (zh) * | 2018-03-14 | 2018-11-13 | 北京理工大学 | 用于检测空间环境中的真菌的引物组合、试剂盒及方法 |
| WO2019200134A2 (en) * | 2018-04-13 | 2019-10-17 | National Agricultural Genotyping Center | Universal lactic acid bacteria quantification kits, methods, compositions and apparatuses therefor |
| CN113260710A (zh) * | 2018-09-24 | 2021-08-13 | 第二基因组股份有限公司 | 用于通过多个定制掺合混合物验证微生物组序列处理和差异丰度分析的组合物、系统、设备和方法 |
| CN109337967A (zh) * | 2018-09-27 | 2019-02-15 | 华中科技大学鄂州工业技术研究院 | 一种实验室的微生物污染鉴别方法 |
| FR3097560B1 (fr) * | 2019-06-24 | 2021-06-25 | Oreal | Méthode de diagnostic d’une exposition chronique à la pollution |
| CN111209895B (zh) * | 2020-02-14 | 2022-03-25 | 李淑媛 | 静脉药物配制样品的细菌污染检测系统、方法及存储介质 |
| CN112575097B (zh) * | 2020-10-12 | 2022-05-10 | 南开大学 | 一种用于检测蜡样芽孢杆菌的液相芯片及应用 |
| WO2022081815A1 (en) * | 2020-10-14 | 2022-04-21 | Pickens Peter Vincent | Optimized detection of blood-borne microbes |
| JP7553323B2 (ja) | 2020-10-30 | 2024-09-18 | 株式会社Kri | スタフィロコッカス細菌の同定方法 |
| CN112226528B (zh) * | 2020-12-14 | 2021-03-16 | 臻和(北京)生物科技有限公司 | 一种用于生物组织样本检测细菌污染的质检方法 |
| JP2024535736A (ja) * | 2021-09-03 | 2024-10-02 | マイクロノーマ，インク． | がん関連微生物バイオマーカーを特定する方法 |
| CN114480596A (zh) * | 2022-02-11 | 2022-05-13 | 宁波市中心血站 | 一种微流控血液及血制品细菌污染检测方法 |
Family Cites Families (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5322770A (en) | 1989-12-22 | 1994-06-21 | Hoffman-Laroche Inc. | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| US5310652A (en) | 1986-08-22 | 1994-05-10 | Hoffman-La Roche Inc. | Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| FI911152A7 (fi) | 1989-07-11 | 1991-03-07 | Gen Probe Inc | Nukleiinihapposekvenssin amplifikaatiomenetelmät |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5270184A (en) | 1991-11-19 | 1993-12-14 | Becton, Dickinson And Company | Nucleic acid target generation |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| DE69435060T2 (de) | 1993-11-12 | 2009-01-02 | Phri Properties, Inc. | Hybridisierungsprobe zur nukleinsäuredetektion, universelle stammstrukturen, methoden und reagentiensätze |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| CA2150986C (en) | 1994-06-17 | 1999-11-02 | Mary Kathryn Meyer | Oligonucleotide primers and probes for detection of bacteria |
| US5641658A (en) | 1994-08-03 | 1997-06-24 | Mosaic Technologies, Inc. | Method for performing amplification of nucleic acid with two primers bound to a single solid support |
| US5705628A (en) | 1994-09-20 | 1998-01-06 | Whitehead Institute For Biomedical Research | DNA purification and isolation using magnetic particles |
| US5710029A (en) | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
| US5705365A (en) | 1995-06-07 | 1998-01-06 | Gen-Probe Incorporated | Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| EP0892808B1 (en) | 1996-04-12 | 2008-05-14 | PHRI Properties, Inc. | Detection probes, kits and assays |
| US20100267012A1 (en) * | 1997-11-04 | 2010-10-21 | Bergeron Michel G | Highly conserved genes and their use to generate probes and primers for detection of microorganisms |
| WO1998044151A1 (en) | 1997-04-01 | 1998-10-08 | Glaxo Group Limited | Method of nucleic acid amplification |
| AU9220498A (en) | 1997-09-04 | 1999-03-22 | Bayer Corporation | Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US6410278B1 (en) | 1998-11-09 | 2002-06-25 | Eiken Kagaku Kabushiki Kaisha | Process for synthesizing nucleic acid |
| ATE262595T1 (de) | 1999-09-13 | 2004-04-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen |
| EP2322667A3 (en) * | 1999-09-28 | 2011-10-26 | Geneohm Sciences Canada, Inc. | Highly conserved gene and its use to generate species-specific, genus-specific, family-specific, group-specific and universal nucleic acid probes for microorganisms. |
| US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
| AUPQ909000A0 (en) | 2000-07-28 | 2000-08-24 | University Of Sydney, The | A method of detecting microorganisms |
| US7001724B1 (en) | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
| JP4632788B2 (ja) | 2002-09-20 | 2011-02-16 | ニュー・イングランド・バイオラブズ・インコーポレイティッド | 核酸のヘリカーゼ依存性増幅 |
| AU2003288018A1 (en) | 2002-11-20 | 2004-06-15 | Pharmacia Italia S.P.A. | Method for the detection of microorganisms in pharmaceutical products |
| CA2508720A1 (en) * | 2002-12-03 | 2004-06-17 | Dartmouth College | Method to measure a t cell response and its uses to qualify antigen-presenting cells |
| US20060046246A1 (en) * | 2003-04-24 | 2006-03-02 | Qiandong Zeng | Genus, group, species and/or strain specific 16S rDNA sequences |
| CA2562221A1 (en) * | 2005-10-21 | 2007-04-21 | Canadian Blood Services | A method for detection of staphylococcus epidermidis |
| WO2008129428A2 (en) | 2007-04-19 | 2008-10-30 | Molecular Detection Inc. | Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria |
| US8877060B2 (en) | 2010-11-23 | 2014-11-04 | Biovec Transfusion, Llc | Methods for removing pathogens from a platelet preparation |
| BR112012009023A2 (pt) * | 2009-10-22 | 2019-09-24 | Biotools Biotechnologial & Medical Laboratories S A | método pra detecção. diferenciação e identificação de eubactérias específica de táxons em uma amostra biológica por meio de técnicas de análise de sequenciamento e kit |
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