CA2911083C - Modified expression of prolyl-4-hydroxylase in physcomitrella patens - Google Patents

Modified expression of prolyl-4-hydroxylase in physcomitrella patens Download PDF

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Publication number
CA2911083C
CA2911083C CA2911083A CA2911083A CA2911083C CA 2911083 C CA2911083 C CA 2911083C CA 2911083 A CA2911083 A CA 2911083A CA 2911083 A CA2911083 A CA 2911083A CA 2911083 C CA2911083 C CA 2911083C
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CA
Canada
Prior art keywords
plant
prolyl
gene
hydroxylase
hydroxylation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2911083A
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English (en)
French (fr)
Other versions
CA2911083A1 (en
Inventor
Ralf Reski
Juliana PARSONS
Friedrich Altmann
Manuela GRAF
Eva Decker
Johannes STADLMANN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitaet fuer Bodenkultur Wien BOKU
Albert Ludwigs Universitaet Freiburg
Original Assignee
Universitaet fuer Bodenkultur Wien BOKU
Albert Ludwigs Universitaet Freiburg
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Filing date
Publication date
Application filed by Universitaet fuer Bodenkultur Wien BOKU, Albert Ludwigs Universitaet Freiburg filed Critical Universitaet fuer Bodenkultur Wien BOKU
Publication of CA2911083A1 publication Critical patent/CA2911083A1/en
Application granted granted Critical
Publication of CA2911083C publication Critical patent/CA2911083C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C12Y114/11002—Procollagen-proline dioxygenase (1.14.11.2), i.e. proline-hydroxylase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Diabetes (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Immunology (AREA)
CA2911083A 2013-05-06 2014-05-05 Modified expression of prolyl-4-hydroxylase in physcomitrella patens Expired - Fee Related CA2911083C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1308120.3 2013-05-06
GBGB1308120.3A GB201308120D0 (en) 2013-05-06 2013-05-06 Recombinant protein and method for its production
PCT/EP2014/059132 WO2014180793A1 (en) 2013-05-06 2014-05-05 Modified expression of prolyl-4-hydroxylase in physcoitrella patens

Publications (2)

Publication Number Publication Date
CA2911083A1 CA2911083A1 (en) 2014-11-13
CA2911083C true CA2911083C (en) 2020-09-15

Family

ID=48627341

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2911083A Expired - Fee Related CA2911083C (en) 2013-05-06 2014-05-05 Modified expression of prolyl-4-hydroxylase in physcomitrella patens

Country Status (16)

Country Link
US (1) US10174334B2 (pt)
EP (1) EP2994479B1 (pt)
JP (1) JP6588892B2 (pt)
KR (1) KR101906442B1 (pt)
AU (1) AU2014264732B2 (pt)
BR (1) BR112015027982A2 (pt)
CA (1) CA2911083C (pt)
DK (1) DK2994479T3 (pt)
ES (1) ES2744993T3 (pt)
GB (1) GB201308120D0 (pt)
HR (1) HRP20191556T1 (pt)
LT (1) LT2994479T (pt)
PL (1) PL2994479T3 (pt)
PT (1) PT2994479T (pt)
SI (1) SI2994479T1 (pt)
WO (1) WO2014180793A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2023513307A (ja) * 2020-02-14 2023-03-30 モダン メドウ，インコーポレイテッド アミノ酸をヒドロキシル化するための単量体タンパク質及び生成物

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001029242A2 (en) * 1999-10-21 2001-04-26 Monsanto Company Post-translational modification of recombinant proteins produced in plants
KR100389141B1 (ko) 2000-05-18 2003-06-25 동아제약 주식회사 인간 에리스로포이에틴을 고효율로 발현하는 재조합세포주 및 이의 제조방법
CN101065491B (zh) 2004-09-29 2012-01-11 胶原植物有限公司 生产胶原的植物及其生成和使用方法
WO2007006570A2 (en) 2005-07-12 2007-01-18 Greenovation Biotech Gmbh Improvements in or relating to protein production

Also Published As

Publication number Publication date
AU2014264732B2 (en) 2017-04-13
ES2744993T3 (es) 2020-02-27
PT2994479T (pt) 2019-09-30
SI2994479T1 (sl) 2019-12-31
WO2014180793A1 (en) 2014-11-13
LT2994479T (lt) 2019-10-25
US10174334B2 (en) 2019-01-08
HRP20191556T1 (hr) 2019-11-29
DK2994479T3 (da) 2019-09-23
JP2016519136A (ja) 2016-06-30
PL2994479T3 (pl) 2020-02-28
GB201308120D0 (en) 2013-06-12
KR20150143593A (ko) 2015-12-23
BR112015027982A2 (pt) 2017-10-17
CA2911083A1 (en) 2014-11-13
AU2014264732A1 (en) 2015-12-03
EP2994479A1 (en) 2016-03-16
KR101906442B1 (ko) 2018-12-07
EP2994479B1 (en) 2019-06-19
US20160208275A1 (en) 2016-07-21
JP6588892B2 (ja) 2019-10-09

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