CA2905842C - Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor - Google Patents

Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor Download PDF

Info

Publication number
CA2905842C
CA2905842C CA2905842A CA2905842A CA2905842C CA 2905842 C CA2905842 C CA 2905842C CA 2905842 A CA2905842 A CA 2905842A CA 2905842 A CA2905842 A CA 2905842A CA 2905842 C CA2905842 C CA 2905842C
Authority
CA
Canada
Prior art keywords
cells
combination
inhibitor
stem cells
use according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2905842A
Other languages
English (en)
French (fr)
Other versions
CA2905842A1 (en
Inventor
Jeffrey Michael Karp
Xiaolei YIN
Marc David Succi
Robert Samuel Langer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brigham and Womens Hospital Inc
Massachusetts Institute of Technology
Original Assignee
Brigham and Womens Hospital Inc
Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brigham and Womens Hospital Inc, Massachusetts Institute of Technology filed Critical Brigham and Womens Hospital Inc
Priority to CA3184040A priority Critical patent/CA3184040A1/en
Publication of CA2905842A1 publication Critical patent/CA2905842A1/en
Application granted granted Critical
Publication of CA2905842C publication Critical patent/CA2905842C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C12N5/068—Stem cells; Progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/065—Modulators of histone acetylation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/42—Notch; Delta; Jagged; Serrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA2905842A 2013-03-14 2014-03-11 Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor Active CA2905842C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3184040A CA3184040A1 (en) 2013-03-14 2014-03-11 Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361783245P 2013-03-14 2013-03-14
US61/783,245 2013-03-14
PCT/US2014/023197 WO2014159356A1 (en) 2013-03-14 2014-03-11 Compositions and methods for epithelial stem cell expansion and culture

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3184040A Division CA3184040A1 (en) 2013-03-14 2014-03-11 Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor

Publications (2)

Publication Number Publication Date
CA2905842A1 CA2905842A1 (en) 2014-10-02
CA2905842C true CA2905842C (en) 2023-02-21

Family

ID=51625153

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2905842A Active CA2905842C (en) 2013-03-14 2014-03-11 Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor
CA3184040A Pending CA3184040A1 (en) 2013-03-14 2014-03-11 Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3184040A Pending CA3184040A1 (en) 2013-03-14 2014-03-11 Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor

Country Status (23)

Country Link
US (4) US10041046B2 (enExample)
EP (3) EP3702443B1 (enExample)
JP (3) JP6469643B2 (enExample)
KR (1) KR102168088B1 (enExample)
CN (2) CN105358677B (enExample)
AU (1) AU2014240603B2 (enExample)
BR (1) BR112015023261A2 (enExample)
CA (2) CA2905842C (enExample)
CY (1) CY1122934T1 (enExample)
DK (2) DK3702443T3 (enExample)
ES (2) ES2908026T3 (enExample)
HR (1) HRP20200692T1 (enExample)
HU (2) HUE049377T2 (enExample)
IL (1) IL241572B (enExample)
LT (1) LT2970890T (enExample)
NZ (1) NZ713219A (enExample)
PL (2) PL3702443T3 (enExample)
PT (2) PT3702443T (enExample)
RS (1) RS60371B1 (enExample)
SI (1) SI2970890T1 (enExample)
SM (1) SMT202000223T1 (enExample)
WO (1) WO2014159356A1 (enExample)
ZA (1) ZA201904258B (enExample)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2669693C (en) 2006-11-15 2018-06-12 Massachusetts Eye & Ear Infirmary Generation of inner ear cells
EP3061808B1 (en) 2009-02-03 2020-08-12 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
WO2011140441A2 (en) 2010-05-06 2011-11-10 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
AU2013312358B2 (en) 2012-09-07 2018-08-09 Massachusetts Eye & Ear Infirmary Treating hearing loss
JP6495174B2 (ja) 2012-12-06 2019-04-03 ステムバイオス テクノロジーズ，インコーポレイテッド Ｌｇｒ５＋体性幹細胞
CA2905842C (en) 2013-03-14 2023-02-21 The Brigham And Women's Hospital, Inc. Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor
US10597633B2 (en) 2014-05-16 2020-03-24 Koninklijke Nederlandse Akademie Van Wetenschappen Culture method for organoids
EP3149156B1 (en) 2014-05-28 2021-02-17 Children's Hospital Medical Center Methods and systems for converting precursor cells into gastric tissues through directed differentiation
US20170314027A1 (en) 2014-08-06 2017-11-02 Massachusetts Eye And Ear Infirmary Increasing atoh1 life to drive sensorineural hair cell differentiantion
WO2016037016A1 (en) 2014-09-03 2016-03-10 The Brigham And Women's Hospital, Inc. Compositions, systems, and methods for generating inner ear hair cells for treatment of hearing loss
US11584916B2 (en) 2014-10-17 2023-02-21 Children's Hospital Medical Center Method of making in vivo human small intestine organoids from pluripotent stem cells
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
US10926001B2 (en) * 2014-12-02 2021-02-23 Polarityte, Inc. Methods related to minimally polarized functional units
EP3328400A4 (en) * 2015-07-28 2019-03-27 Stembios Technologies, Inc. COMPOSITION AND METHOD FOR INHIBITING HISTONATE ACETYLASE
KR101756337B1 (ko) 2015-08-12 2017-07-10 고려대학교 산학협력단 Cxcr2 억제를 통한 인간 전분화능 줄기세포의 내배엽 및 중배엽 분화 방법
US11185536B2 (en) 2015-12-04 2021-11-30 Massachusetts Eye And Ear Infirmary Treatment of hearing loss by inhibition of casein kinase 1
US11021687B2 (en) 2016-01-08 2021-06-01 The Brigham And Women's Hospital, Inc. Production of differentiated enteroendocrine cells and insulin producing cells
US20190018000A1 (en) 2016-01-12 2019-01-17 Cedars-Sinai Medical Center A method of non destructive monitoring of biological processes in microfluidic tissue culture systems
CN109219439B (zh) 2016-01-29 2022-09-20 马萨诸塞眼科耳科诊所 内耳支持细胞的扩增和分化及其使用方法
EP3411470A4 (en) 2016-02-01 2019-10-09 Emulate, Inc. SYSTEMS AND METHOD FOR BREEDING DARM CELLS IN MICROFLUIDIC DEVICES
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
US10016507B2 (en) 2016-03-02 2018-07-10 Frequency Therapeutics, Inc. Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using GSK3 inhibitors: III
AU2017227846A1 (en) * 2016-03-02 2018-08-30 Frequency Therapeutics, Inc. Methods for controlled proliferation of vestibular stem cells / generating inner ear hair cells using WNT and TGF-beta inhibition
US10201540B2 (en) 2016-03-02 2019-02-12 Frequency Therapeutics, Inc. Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using GSK3 inhibitors: I
US9968615B2 (en) 2016-03-02 2018-05-15 Frequency Therapeutics, Inc. Methods for controlled proliferation of stem cells / generating inner ear hair cells using 3-(pyridin-2-yl)-1H-indol-2-ol based compounds
US9913848B2 (en) 2016-03-02 2018-03-13 Frequency Therapeutics, Inc. Methods for controlled proliferation of stem cells / generating inner ear hair cells using 1,2,3,4-tetrahydro-[1,4]diazepino[6,7, 1-hi]indolyl based compounds
US10213511B2 (en) 2016-03-02 2019-02-26 Frequency Therapeutics, Inc. Thermoreversible compositions for administration of therapeutic agents
US11260130B2 (en) 2016-03-02 2022-03-01 Frequency Therapeutics, Inc. Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using a GSK3 inhibitor: IV
AU2017227844A1 (en) * 2016-03-02 2018-08-30 Frequency Therapeutics, Inc. Methods for controlled proliferation of stem cells / generating inner ear hair cells using GSK-3-alpha inhibitors
US9913835B2 (en) 2016-03-02 2018-03-13 Frequency Therapeutics, Inc. Methods for controlled proliferation of stem cells / generating inner ear hair cells using N-(alkylcarbamoyl)-1H-pyrazol-4-yl)-nicotinamide based compounds
US11479754B2 (en) 2016-04-20 2022-10-25 Kyoto Prefectural Public University Corporation Method for producing cultivated epithelial cell sheet
CN116790476A (zh) * 2016-05-05 2023-09-22 儿童医院医疗中心 用于体外制造胃底组织的方法和与其相关的组合物
CN109415680B (zh) * 2016-05-18 2022-08-16 学校法人庆应义塾 类器官培养用细胞培养基、培养方法及类器官
CA3066107A1 (en) 2016-06-03 2017-12-07 Hough Ear Institute Combination therapies for inner ear sensory hair cell regeneration/replacement
GB201610748D0 (en) * 2016-06-20 2016-08-03 Koninklijke Nederlandse Akademie Van Wetenschappen Improved diffrentation method
KR102546194B1 (ko) 2016-11-04 2023-06-21 칠드런즈 호스피탈 메디칼 센터 간 유사 장기 조성물 및 이를 제조 및 사용하는 방법
KR102026313B1 (ko) 2016-11-10 2019-09-30 울산대학교 산학협력단 삼차원 채널 구조물
EP3323882A1 (en) * 2016-11-18 2018-05-23 Ecole Polytechnique Fédérale de Lausanne Organoid tissue engineering
KR102558606B1 (ko) 2016-12-05 2023-07-26 칠드런즈 호스피탈 메디칼 센터 결장 유사장기 및 이를 제조 및 사용하는 방법
US10383881B2 (en) 2016-12-30 2019-08-20 Frequency Therapeutics, Inc. 1,5-dihydro-2H-pyrrol-2-one compounds and methods of using same
US11066419B2 (en) 2016-12-30 2021-07-20 Frequency Therapeutics, Inc. 1H-pyrrole-2,5-dione compounds and methods of using same
US11913022B2 (en) 2017-01-25 2024-02-27 Cedars-Sinai Medical Center In vitro induction of mammary-like differentiation from human pluripotent stem cells
KR102323928B1 (ko) 2017-02-20 2021-11-08 고리츠다이가쿠호징 나고야시리츠다이가쿠 인공 다능성 줄기 세포 유래 장관 줄기 세포의 유지 배양
US11767513B2 (en) 2017-03-14 2023-09-26 Cedars-Sinai Medical Center Neuromuscular junction
US11414648B2 (en) 2017-03-24 2022-08-16 Cedars-Sinai Medical Center Methods and compositions for production of fallopian tube epithelium
WO2018191673A1 (en) 2017-04-14 2018-10-18 Children's Hospital Medical Center Multi donor stem cell compositions and methods of making same
US20200199660A1 (en) 2017-05-10 2020-06-25 The Broad Institute, Inc. Methods and compositions for determination of mutations in single replication events
KR101966523B1 (ko) * 2017-05-29 2019-04-05 차의과학대학교 산학협력단 오가노이드 배양을 위한 조성물 및 방법
EP3630949A4 (en) * 2017-05-29 2021-03-10 Stemcell Technologies Canada Inc. COMPOSITIONS AND PROCESSES FOR OBTAINING ORGANOIDS
CN107189981B (zh) * 2017-06-07 2020-01-21 广东医科大学 Hdac6抑制剂在骨髓间充质干细胞移植中的应用
US12105089B2 (en) 2017-07-17 2024-10-01 The Broad Institute, Inc. Cell atlas of the healthy and ulcerative colitis human colon
EP3694603A4 (en) 2017-10-10 2021-07-14 Children's Hospital Medical Center ESOPHAGUS TISSUE AND / OR ORGANOID COMPOSITIONS AND METHOD FOR MANUFACTURING THEREOF
EP3727394A4 (en) 2017-12-21 2021-09-08 Children's Hospital Medical Center DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF
WO2019133810A1 (en) * 2017-12-28 2019-07-04 Tract Pharmaceuticals, Inc. Stem cell culture systems for columnar epithelial stem cells, and uses related thereto
US11994512B2 (en) 2018-01-04 2024-05-28 Massachusetts Institute Of Technology Single-cell genomic methods to generate ex vivo cell systems that recapitulate in vivo biology with improved fidelity
CN108085296B (zh) * 2018-01-29 2021-04-13 清华大学 肠上皮类器官无生长因子培养方法
US12161676B2 (en) 2018-03-23 2024-12-10 Cedars-Sinai Medical Center Methods of use of islet cells
WO2019195798A1 (en) 2018-04-06 2019-10-10 Cedars-Sinai Medical Center Human pluripotent stem cell derived neurodegenerative disease models on a microfluidic chip
WO2019195800A1 (en) 2018-04-06 2019-10-10 Cedars-Sinai Medical Center Novel differentiation technique to generate dopaminergic neurons from induced pluripotent stem cells
CN110205283B (zh) * 2018-04-18 2021-04-23 浙江大学 一种诱导人羊膜上皮细胞向视网膜色素上皮细胞分化的方法及其应用
KR102099402B1 (ko) * 2018-06-28 2020-04-09 오가노이드사이언스 주식회사 타액선 줄기세포의 기능 강화용 배양 조성물 및 이를 이용한 타액선 줄기세포 기능 강화 방법
JP7506657B2 (ja) 2018-07-26 2024-06-26 チルドレンズ ホスピタル メディカル センター 肝胆膵組織およびその作製方法
EP3837351A1 (en) 2018-08-17 2021-06-23 Frequency Therapeutics, Inc. Compositions and methods for generating hair cells by downregulating foxo
CN113195707A (zh) 2018-08-17 2021-07-30 频率治疗公司 用于通过上调jag-1来生成毛细胞的组合物和方法
US12428622B2 (en) 2018-09-12 2025-09-30 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
US20210395679A1 (en) * 2018-11-09 2021-12-23 Children's Hospital Medical Center In vitro cell culture system for producing hepatocyte-like cells and uses thereof
GB201819224D0 (en) 2018-11-26 2019-01-09 Koninklijke Nederlandse Akademie Van Wetenschappen Hepatocyte expansion methods
WO2020112883A1 (en) * 2018-11-30 2020-06-04 Massachusetts Eye And Ear Infirmary Inhibition of lysine demethylase 1 (lsd1) induces differentiation of hair cells
CN109576225A (zh) * 2018-12-23 2019-04-05 山西医科大学 利用Rspo1抑制骨髓间充质干细胞凋亡的方法
CN109679893A (zh) * 2019-01-17 2019-04-26 浙江工商大学 基于小鼠肠道干细胞的肠上皮单层培养及表征方法
WO2020159609A1 (en) * 2019-01-30 2020-08-06 MEAD, Benjamin Elliott Small molecule enhancers of paneth cell function and differentiation
KR102050223B1 (ko) * 2019-04-04 2019-11-29 포항공과대학교 산학협력단 배아줄기세포로부터 소장 오가노이드의 제조방법
KR102334245B1 (ko) * 2019-05-29 2021-12-03 연세대학교 산학협력단 인간 미뢰 오가노이드의 배양용 조성물
JP7610533B2 (ja) 2019-05-31 2025-01-08 チルドレンズ ホスピタル メディカル センター 造血幹細胞の生成および拡大方法
WO2020247836A1 (en) * 2019-06-07 2020-12-10 Massachusetts Institute Of Technology Methods and compositions for differentiating stem cells
EP3789049A1 (en) * 2019-09-06 2021-03-10 QGel SA Method for obtaining healthy intestinal organoids
WO2021081253A1 (en) 2019-10-22 2021-04-29 Tempus Labs, Inc. Systems and methods for predicting therapeutic sensitivity
US11629385B2 (en) 2019-11-22 2023-04-18 Tempus Labs, Inc. Tumor organoid culture compositions, systems, and methods
EP4070098B1 (en) 2019-12-05 2025-12-24 Tempus AI, Inc. Large scale organoid analysis
CA3165424A1 (en) 2019-12-20 2021-06-24 Tenaya Therapeutics, Inc. Fluoroalkyl-oxadiazoles and uses thereof
KR102315866B1 (ko) * 2020-03-24 2021-10-21 한국원자력의학원 섬유화 질환 복합 치료제 개발
CN111471643B (zh) * 2020-04-09 2020-12-29 创芯国际生物科技(广州)有限公司 一种通用型上呼吸道粘膜类器官的培养基及培养方法
US11561178B2 (en) 2020-04-20 2023-01-24 Tempus Labs, Inc. Artificial fluorescent image systems and methods
CN111411083B (zh) * 2020-04-22 2020-11-03 创芯国际生物科技(广州)有限公司 一种胃癌类器官的培养基及培养方法
CN111849860B (zh) * 2020-06-01 2021-12-21 浙江大学 一种利用铁元素调控肠道干细胞分化的方法及应用
CN112592896B (zh) * 2020-11-22 2024-06-25 深圳市第二人民医院(深圳市转化医学研究院) 一种肺腺癌类器官的培养液及其培养方法
JP7602932B2 (ja) * 2021-02-26 2024-12-19 株式会社マンダム 汗腺筋上皮細胞の幹細胞性維持剤、汗腺筋上皮細胞の幹細胞性を維持する方法および皮膚外用剤
EP4326263A1 (en) 2021-04-23 2024-02-28 Tenaya Therapeutics, Inc. Hdac6 inhibitors for use in the treatment of dilated cardiomyopathy
TW202308619A (zh) 2021-05-04 2023-03-01 美商特納亞治療股份有限公司 用於治療代謝疾病和hfpef的hdac6抑制劑
WO2022261293A1 (en) * 2021-06-09 2022-12-15 The Regents Of The University Of California Lung organoid model and method of use
KR20230073997A (ko) 2021-11-19 2023-05-26 연세대학교 산학협력단 인간 타액선 상피성 줄기세포 또는 전구세포 유래 단일클론세포 분리, 유지, 증식 및 분화 방법 및 타액선 질환 치료용 세포외소포체 생산 방법
US20250034523A1 (en) * 2021-12-09 2025-01-30 The Trustees Of Indiana University Methods of generating human cochlear hair cells
CN114480274A (zh) * 2021-12-30 2022-05-13 中国人民解放军海军军医大学第一附属医院 肠道类器官定向分化潘氏细胞的方法
CN115637251A (zh) * 2022-11-04 2023-01-24 中国人民解放军海军军医大学第一附属医院 一种诱导肠道类器官定向分化为肠吸收细胞的方法及其应用
KR102693565B1 (ko) * 2022-11-30 2024-08-08 오가노이드사이언스 주식회사 암 오가노이드 제조용 배지 조성물
CN116478912A (zh) 2023-06-07 2023-07-25 合肥工业大学 一种诱导小鼠肠道类器官定向分化为肠内分泌细胞的培养基及其应用
WO2025152110A1 (zh) * 2024-01-18 2025-07-24 五邑大学 用于阿尔兹海默症治疗的联合用药及其药物组合物
WO2025215092A1 (en) 2024-04-10 2025-10-16 Institut National de la Santé et de la Recherche Médicale Selective hdac6 inhibitors for use in the treatment of myotonic dystrophy type 1

Family Cites Families (117)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5059591B1 (en) 1983-05-26 2000-04-25 Liposome Co Inc Drug preparations of reduced toxicity
US5571714A (en) 1988-12-22 1996-11-05 Celtrix Pharmaceuticals, Inc. Monoclonal antibodies which bind both transforming growth factors β1 and β2 and methods of use
US5731144A (en) 1990-06-11 1998-03-24 Nexstar Pharmaceuticals, Inc. High affinity TGFβ nucleic acid ligands
US5731424A (en) 1990-06-11 1998-03-24 Nexstar Pharmaceuticals, Inc. High affinity TGFβ nucleic acid ligands and inhibitors
US6124449A (en) 1990-06-11 2000-09-26 Nexstar Pharmaceuticals, Inc. High affinity TGFβ nucleic acid ligands and inhibitors
US5561163A (en) 1990-08-31 1996-10-01 Deprenyl Animal Health, Inc. Treating hearing loss with deprenyl
US5421818A (en) 1993-10-18 1995-06-06 Inner Ear Medical Delivery Systems, Inc. Multi-functional inner ear treatment and diagnostic system
GB9601081D0 (en) 1995-10-06 1996-03-20 Cambridge Antibody Tech Specific binding members for human transforming growth factor beta;materials and methods
US5837681A (en) 1996-02-23 1998-11-17 Amgen Inc. Method for treating sensorineural hearing loss using glial cell line-derived neurotrophic factor (GDNF) protein product
US6593290B1 (en) 1996-11-01 2003-07-15 Genentech, Inc. Treatment of inner ear hair cells
US6045528A (en) 1997-06-13 2000-04-04 Intraear, Inc. Inner ear fluid transfer and diagnostic system
US6177434B1 (en) 1997-12-16 2001-01-23 The United States Of America As Represented By The Secretary Of The Navy Prevention or reversal of sensorineural hearing loss (SNHL) through biologic mechanisms
US6806358B1 (en) 1997-12-16 2004-10-19 University Of California San Diego Peptide inhibitor of TGF-β growth factors
US6943191B1 (en) 1998-02-27 2005-09-13 The United States Of America As Represented By The Department Of Health And Human Services Disubstituted lavendustin A analogs and pharmaceutical composition comprising the analogs
GB9809869D0 (en) 1998-05-09 1998-07-08 Medical Res Council Inhibition of protein kinases
US6184226B1 (en) 1998-08-28 2001-02-06 Scios Inc. Quinazoline derivatives as inhibitors of P-38 α
ES2146552B1 (es) 1998-11-24 2001-04-16 Inst Cientifico Tecnol Navarra Peptidos inhibidores de tgf/31
AU4206200A (en) 1999-04-05 2000-10-23 Adherex Technologies Inc. Compounds and methods for stimulating beta-catenin mediated gene expression and differentiation
US6492497B1 (en) 1999-04-30 2002-12-10 Cambridge Antibody Technology Limited Specific binding members for TGFbeta1
CZ296087B6 (cs) 2000-05-11 2006-01-11 Consejo Superior Investigaciones Cientificas Farmaceutický prostredek pro lécbu nemoci, na nízse podílí heterocyklické inhibitory glykogensyntázakinázy GSK-3
US6509318B1 (en) 2000-09-29 2003-01-21 The Regents Of The University Of California TGF-B inhibitors and methods
US20050227936A1 (en) 2001-05-18 2005-10-13 Sirna Therapeutics, Inc. RNA interference mediated inhibition of TGF-beta and TGF-beta receptor gene expression using short interfering nucleic acid (siNA)
US20050287128A1 (en) 2001-05-18 2005-12-29 Sirna Therapeutics, Inc. RNA interference mediated inhibition of TGF-beta and TGF-beta receptor gene expression using short interfering nucleic acid (siNA)
KR100861630B1 (ko) 2001-05-24 2008-10-07 일라이 릴리 앤드 캄파니 제약 제제로서의 신규 피롤 유도체
US20030028905A1 (en) 2001-07-31 2003-02-06 Petra Knaus Mutant forms of the TGF-beta type II receptor which bind all TGF-beta isoforms
MXPA04004178A (es) 2001-11-01 2004-09-06 Janssen Pharmaceutica Nv Heteroarilaminas como inhibidores de glucogeno cintasa cinasa 3beta.
SE0200979D0 (sv) 2002-03-28 2002-03-28 Astrazeneca Ab New compounds
US20040006030A1 (en) 2002-07-02 2004-01-08 Isis Pharmaceuticals Inc. Antisense modulation of TGF-beta 2 expression
EP1506191A1 (en) 2002-05-15 2005-02-16 Smithkline Beecham Corporation Benzoxazine and benzoxazinone substituted triazoles
AU2003229305A1 (en) 2002-05-17 2003-12-02 Scios, Inc. TREATMENT OF FIBROPROLIFERATIVE DISORDERS USING TGF-Beta INHIBITORS
US7190468B2 (en) 2002-07-17 2007-03-13 Hewlett-Packard Development Company, L.P. Background document rendering system and method
AU2003260345A1 (en) 2002-07-31 2004-02-23 Smithkline Beecham Corporation 2-phenylpyridin-4-yl derivatives as alk5 inhibitors
ATE339419T1 (de) 2002-08-02 2006-10-15 Vertex Pharma Pyrazolenthaltende zusammensetzungen und ihre verwendung als gsk-3 inhibitoren
EP1739087A1 (en) 2002-08-02 2007-01-03 Vertex Pharmaceuticals Incorporated Pyrazole compositions useful as inhibitors of gsk-3
UA80296C2 (en) 2002-09-06 2007-09-10 Biogen Inc Imidazolopyridines and methods of making and using the same
DE60315677T2 (de) 2002-09-17 2008-06-05 Eli Lilly And Co., Indianapolis Pyrazolopyridin derivate als tgf beta hemmstoffe zur behandlung von krebs
OA12925A (en) 2002-09-18 2006-10-13 Pfizer Prod Inc Novel isothiazole and isoxazole compounds as transforming growth factor (TGF) inhibitors.
AU2003260810A1 (en) 2002-09-18 2004-04-08 Pfizer Products Inc. Triazole derivatives as transforming growth factor (tgf) inhibitors
AU2003291149A1 (en) 2002-11-22 2004-06-18 Scios, Inc. USE OF TFG-Beta INHIBITORSTO COUNTERACT PATHOLOGIC CHANGES IN THE LEVEL OR FUNCTION OF STEROID/THYROID RECEPTORS
SE0203752D0 (sv) 2002-12-17 2002-12-17 Astrazeneca Ab New compounds
JP2006516603A (ja) 2003-01-27 2006-07-06 ファイザー・プロダクツ・インク イソチアゾール誘導体
ES2305744T3 (es) 2003-03-12 2008-11-01 Millennium Pharmaceuticals, Inc. Derivados de quinazolina como inhibidores e tgf-beta.
US7223766B2 (en) 2003-03-28 2007-05-29 Scios, Inc. Bi-cyclic pyrimidine inhibitors of TGFβ
WO2004112710A2 (en) 2003-06-17 2004-12-29 Millennium Pharmaceuticals, Inc. COMPOSITIONS AND METHODS FOR INHIBITING TGF-ß
GB0316756D0 (en) 2003-07-17 2003-08-20 Bp Chem Int Ltd Process
WO2005009287A2 (en) 2003-07-21 2005-02-03 University Of Maryland, Baltimore Drug delivery to the inner ear and methods of using same
SG145713A1 (en) 2003-08-13 2008-09-29 Univ Illinois SILENCING OF TGFss TYPE II RECEPTOR EXPRESSION BY SIRNA
WO2005039570A1 (ja) 2003-10-28 2005-05-06 Riken ＴＧＦ－β情報伝達経路阻害剤
US8673634B2 (en) 2003-11-13 2014-03-18 Massachusetts Eye & Ear Infirmary Method for the treatment of hearing loss
GB0327908D0 (en) 2003-12-02 2004-01-07 Neuropharma Sa GSK-3 inhibitors isolated from marine organisms
US20050245508A1 (en) 2003-12-24 2005-11-03 Scios, Inc. Treatment of malignant gliomas with TGF-beta inhibitors
EP1723146A1 (en) 2004-03-01 2006-11-22 Eli Lilly And Company Fused pyrazole derivatives as tgf-beta signal transduction inhibitors for the treatment of fibrosis and neoplasms
AR048335A1 (es) 2004-03-24 2006-04-19 Chugai Pharmaceutical Co Ltd Agentes terapeuticos para trastornos del oido interno que contienen un antagonista de il- 6 como un ingrediente activo
WO2005115527A2 (en) 2004-05-24 2005-12-08 Auris Medical, Llc. Combined otic aspirator and medication dispenser
GB0418328D0 (en) 2004-08-17 2004-09-22 Imp College Innovations Ltd Cancer methods and medicaments
JP2007176795A (ja) 2004-08-18 2007-07-12 Univ Nihon ＴＧＦ−β遺伝子発現抑制剤
WO2006034207A2 (en) 2004-09-17 2006-03-30 Vanderbilt University Use of gsk3 inhibitors in combination with radiation therapies
MX2007009545A (es) 2005-02-08 2008-03-11 Genzyme Corp Anticuerpos para tgfbeta.
GB0505970D0 (en) 2005-03-23 2005-04-27 Univ Edinburgh Culture medium containing kinase inhibitor, and uses thereof
US20070066632A1 (en) 2005-03-25 2007-03-22 Scios, Inc. Fused bicyclic inhibitors of TGFbeta
ES2387402T3 (es) 2005-03-31 2012-09-21 Stemnion, Inc. Composiciones celulares derivadas del amnios, procedimientos de fabricación y usos de las mismas
DE602006008474D1 (de) 2005-06-30 2009-09-24 Janssen Pharmaceutica Nv Toren
DK1910370T3 (en) 2005-07-22 2015-06-08 Lilly Co Eli PYRIDINQUINOLINE SUBSTITUTED PYRROLO [1,2-B] PYRAZOLE MONOHYDRATE AS TGF-BETA INHIBITOR
EP1749523A1 (en) 2005-07-29 2007-02-07 Neuropharma, S.A. GSK-3 inhibitors
EP1974740A4 (en) 2005-10-24 2010-09-01 Proyecto Biomedicina Cima Sl USE OF TGF-BETA1 INHIBITORY PEPTIDES IN THE PREPARATION OF AN IMMUNE RESPONSE MODULATING AGENT
US7951845B2 (en) 2006-01-19 2011-05-31 The Regents Of The University Of Michigan Composition and method of treating hearing loss
ITMI20060181A1 (it) 2006-02-03 2007-08-04 Univ Padova Modulatori del tgf-b e loro uso
TW200800203A (en) 2006-03-08 2008-01-01 Astrazeneca Ab New use
US7846908B2 (en) 2006-03-16 2010-12-07 Alnylam Pharmaceuticals, Inc. RNAi modulation of TGF-beta and therapeutic uses thereof
ATE530540T1 (de) 2006-08-23 2011-11-15 Pfizer Prod Inc Pyrimidonverbindungen als gsk-3-inhibitoren
CA2669693C (en) 2006-11-15 2018-06-12 Massachusetts Eye & Ear Infirmary Generation of inner ear cells
ES2446269T3 (es) 2006-12-19 2014-03-06 The Board Of Trustees Of The University Of Illinois 3-Benzofuranil-4-indolil-maleimidas como potentes inhibidores de GSK-3 para trastornos neurodegenerativos
WO2009017453A1 (en) 2007-07-30 2009-02-05 Astrazeneca Ab New therapeutic combination of an antipsychotic and a gsk3 inhibitor 958
WO2009017455A1 (en) 2007-07-30 2009-02-05 Astrazeneca Ab A new combination of (a) an alpha-4-beta-2 -neuronal nicotinic agonist and (b) a gsk3 inhibitor
US20110190280A1 (en) 2007-08-29 2011-08-04 George Adjabeng Thiazole And Oxazole Kinase Inhibitors
WO2009035522A1 (en) 2007-09-14 2009-03-19 Albert Einstein College Of Medicine Of Yeshiva University Use of gamma secretase inhibitors and notch pathway inhibitors for treatment and prevention of renal disease
US9534205B2 (en) 2008-03-17 2017-01-03 The Scripps Research Institute Combined chemical and genetic approaches for generation of induced pluripotent stem cells
EP2278999B1 (en) 2008-04-21 2025-01-29 Dompé farmaceutici S.p.A. Auris formulations for treating otic diseases and conditions
US20090270497A1 (en) * 2008-04-24 2009-10-29 Pharmacyclics, Inc. Treatment of Non-Localized Inflammation with pan-HDAC Inhibitors
WO2009142719A2 (en) 2008-05-19 2009-11-26 Massachusetts Institute Of Technology Tympanic membrane permeating ear drops and uses thereof
US8496957B2 (en) 2008-07-21 2013-07-30 Otonomy, Inc Controlled release auris sensory cell modulator compositions and methods for the treatment of otic disorders
US8298825B1 (en) 2008-08-25 2012-10-30 The General Hospital Corporation TGF-beta receptor inhibitors to enhance direct reprogramming
US20120059021A1 (en) 2008-11-11 2012-03-08 University Of Washington Compositions and methods for treating cancer and methods for predicting a response to such treatments
CA2743436C (en) 2008-11-24 2017-10-31 Massachusetts Eye & Ear Infirmary Pathways to generate hair cells
EP2376626A4 (en) 2008-12-13 2012-10-17 Dna Microarray MICRO-ENVIRONMENTAL NICHE ASSAY FOR SCREENING OF INDUCED PLURIPOTENT STEM CELLS (CIPS)
US20130039998A1 (en) 2008-12-24 2013-02-14 University Of Washington Compositions of modulators of the wnt/beta-catenin pathway and benzamide and/or hydroxamic acid derivatives to treat bipolar disorder
EP3061808B1 (en) * 2009-02-03 2020-08-12 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells
US9752124B2 (en) * 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
GB201111244D0 (en) * 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
CN102421784B (zh) 2009-03-11 2015-09-30 杏林制药株式会社 作为gsk-3抑制剂的7-环烷基氨基喹诺酮
WO2011019957A1 (en) 2009-08-12 2011-02-17 University Of Southern California Method for formation of induced pluripotent stem cells
MX390753B (es) * 2009-10-31 2025-03-21 Genesis Tech Limited Metodos para reprogramar celulas y usos de los mismos.
US20110135756A1 (en) 2009-12-08 2011-06-09 University Of Washington Compositions and methods for protecting sensory hair cells
RU2012132429A (ru) 2009-12-28 2014-02-10 Аудиокьюр Фарма Гмбх Бета-карболины для лечения нарушения слуха и головокружения
RU2012135093A (ru) 2010-01-19 2014-03-10 Астразенека Аб Производные пиразина
WO2011116930A1 (en) * 2010-03-22 2011-09-29 Cellartis Ab Directed differentiation and maturation of pluripotent cells into hepatocyte like cells by modulation of wnt-signalling pathway
WO2011143511A2 (en) 2010-05-12 2011-11-17 Columbia University Methods for producing enteroendocrine cells that make and secrete insulin
CN103459591B (zh) 2010-11-02 2016-05-04 国立大学法人熊本大学 肠细胞的制造方法
WO2012103012A1 (en) 2011-01-24 2012-08-02 The Board Of Trustees Of The Leland Stanford Junior University Methods for generating inner ear cells in vitro
KR101807704B1 (ko) * 2011-04-12 2017-12-13 차의과학대학교 산학협력단 역분화-증진제를 이용한 역분화 만능 줄기세포의 제조방법
ES2711777T3 (es) 2011-09-14 2019-05-07 Samumed Llc Indazol-3-carboxamidas y su uso como inhibidores de la ruta de señalización de Wnt/b-catenina
JP6041270B2 (ja) 2011-10-06 2016-12-07 学校法人慶應義塾 角膜内皮細胞の製造方法
PL2838888T3 (pl) 2012-02-24 2017-12-29 Asd Therapeutics Partners Llc Tiadiazolidynodiony jako inhibitory gsk-3
US9457009B2 (en) 2012-05-31 2016-10-04 The United States Of America As Represented By The Department Of Veterans Affairs Methods and compositions for preventing and treating auditory dysfunctions
TW201406758A (zh) 2012-06-28 2014-02-16 Daiichi Sankyo Co Ltd 三環性化合物
GB201212960D0 (en) 2012-07-20 2012-09-05 Common Services Agency Erythroid production
AU2013312305B2 (en) 2012-09-07 2018-06-28 Massachusetts Eye And Ear Infirmary Methods and compositions for regenerating hair cells and/or supporting cells
TW201417817A (zh) 2012-09-25 2014-05-16 Daiichi Sankyo Co Ltd Ｇｓｋ３抑制劑與抗ｄｒ５抗體之組合
ES2719776T3 (es) 2012-10-12 2019-07-16 Broad Inst Inc Inhibidores de GSK3 y métodos de uso de los mismos
US10294457B2 (en) 2012-11-29 2019-05-21 Takara Bio Europe Ab Maturation of hepatocyte-like cells derived from human pluripotent stem cells
US20140248696A1 (en) * 2013-03-01 2014-09-04 Wisconsin Alumni Research Foundation Methods of maintaining, expanding, and diffrentiating neuronal subtype specific progenitors
CA2905842C (en) 2013-03-14 2023-02-21 The Brigham And Women's Hospital, Inc. Compositions and methods for epithelial stem cell expansion comprising a wnt agonist and a histone deacetylase inhibitor
WO2015168149A2 (en) 2014-04-28 2015-11-05 Massachusetts Eye & Ear Infirmary Sensorineural hair cell differentiation
WO2015175783A1 (en) 2014-05-15 2015-11-19 International Stem Cell Corporation Chemical differentiation of pluripotentstem cells into retinal epithelial cells
WO2016037016A1 (en) 2014-09-03 2016-03-10 The Brigham And Women's Hospital, Inc. Compositions, systems, and methods for generating inner ear hair cells for treatment of hearing loss
US11021687B2 (en) 2016-01-08 2021-06-01 The Brigham And Women's Hospital, Inc. Production of differentiated enteroendocrine cells and insulin producing cells

Also Published As

Publication number Publication date
JP6469643B2 (ja) 2019-02-13
EP4056677A1 (en) 2022-09-14
AU2014240603A1 (en) 2015-11-05
CN108865972B (zh) 2022-07-29
CN108865972A (zh) 2018-11-23
EP3702443B1 (en) 2022-01-19
BR112015023261A2 (pt) 2017-07-18
JP6919032B2 (ja) 2021-08-11
EP2970890A4 (en) 2016-11-02
US20160194604A1 (en) 2016-07-07
ES2908026T3 (es) 2022-04-27
EP2970890A1 (en) 2016-01-20
HUE057521T2 (hu) 2022-05-28
WO2014159356A1 (en) 2014-10-02
AU2014240603B2 (en) 2017-11-09
PL2970890T3 (pl) 2020-11-16
SMT202000223T1 (it) 2020-05-08
EP3702443A1 (en) 2020-09-02
PT3702443T (pt) 2022-02-17
ES2786925T3 (es) 2020-10-14
LT2970890T (lt) 2020-07-10
HK1219754A1 (en) 2017-04-13
CN105358677B (zh) 2018-06-15
HUE049377T2 (hu) 2020-09-28
DK3702443T3 (da) 2022-04-04
EP2970890B1 (en) 2020-02-12
CY1122934T1 (el) 2020-10-14
US10041047B2 (en) 2018-08-07
RS60371B1 (sr) 2020-07-31
CA3184040A1 (en) 2014-10-02
JP2016510999A (ja) 2016-04-14
KR20160006167A (ko) 2016-01-18
CN105358677A (zh) 2016-02-24
JP6764909B2 (ja) 2020-10-07
ZA201904258B (en) 2023-03-29
US20190017015A1 (en) 2019-01-17
CA2905842A1 (en) 2014-10-02
US10954490B2 (en) 2021-03-23
NZ713219A (en) 2017-04-28
JP2018174947A (ja) 2018-11-15
PL3702443T3 (pl) 2022-05-16
US20170226477A1 (en) 2017-08-10
KR102168088B1 (ko) 2020-10-20
SI2970890T1 (sl) 2020-10-30
HRP20200692T1 (hr) 2020-08-07
IL241572B (en) 2020-10-29
PT2970890T (pt) 2020-04-24
US20210301254A1 (en) 2021-09-30
DK2970890T3 (da) 2020-05-04
JP2020168015A (ja) 2020-10-15
US10041046B2 (en) 2018-08-07

Similar Documents

Publication Publication Date Title
US10954490B2 (en) Compositions and methods for epithelial stem cell expansion and culture
US11021687B2 (en) Production of differentiated enteroendocrine cells and insulin producing cells
US11649431B2 (en) Cortical interneurons and other neuronal cells produced by the directed differentiation of pluripotent and multipotent cells
RU2714256C2 (ru) Культуральная среда стволовых клеток
JP2013536689A (ja) Ｅｍｔを調節するための組成物および方法ならびにその使用
HK40034599B (en) Compositions and methods for epithelial stem cell expansion and culture
HK40034599A (en) Compositions and methods for epithelial stem cell expansion and culture
HK1219754B (en) Compositions and methods for epithelial stem cell expansion and culture
HK1260137B (en) A cell culture solution
HK1260137A1 (en) A cell culture solution
KR20240093530A (ko) 뇌하수체 호르몬 생산 세포를 포함하는 세포 응집체 및 그 생산 방법
HK40109173A (en) Cell aggregate including pituitary hormone-producing cells and method for producing same
CN118318033A (zh) 包含垂体激素产生细胞的细胞聚集体及其制备方法

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20190308

EEER Examination request

Effective date: 20190308

EEER Examination request

Effective date: 20190308

EEER Examination request

Effective date: 20190308