CA2894635C - Methods and primer sets for high throughput pcr sequencing - Google Patents
Methods and primer sets for high throughput pcr sequencing Download PDFInfo
- Publication number
- CA2894635C CA2894635C CA2894635A CA2894635A CA2894635C CA 2894635 C CA2894635 C CA 2894635C CA 2894635 A CA2894635 A CA 2894635A CA 2894635 A CA2894635 A CA 2894635A CA 2894635 C CA2894635 C CA 2894635C
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- CA
- Canada
- Prior art keywords
- sequence
- seq
- primers
- primer
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12199315 | 2012-12-23 | ||
| EP12199315.8 | 2012-12-23 | ||
| EP13175199.2 | 2013-07-04 | ||
| EP13175199.2A EP2746405B1 (en) | 2012-12-23 | 2013-07-04 | Methods and primer sets for high throughput PCR sequencing |
| PCT/EP2013/077763 WO2014096394A1 (en) | 2012-12-23 | 2013-12-20 | Methods and primer sets for high throughput pcr sequencing |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2894635A1 CA2894635A1 (en) | 2014-06-26 |
| CA2894635C true CA2894635C (en) | 2021-03-02 |
Family
ID=47504728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2894635A Active CA2894635C (en) | 2012-12-23 | 2013-12-20 | Methods and primer sets for high throughput pcr sequencing |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10894983B2 (enExample) |
| EP (2) | EP2746405B1 (enExample) |
| JP (1) | JP6496665B2 (enExample) |
| CA (1) | CA2894635C (enExample) |
| DK (1) | DK2935618T3 (enExample) |
| WO (1) | WO2014096394A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104562214A (zh) * | 2014-12-26 | 2015-04-29 | 上海派森诺生物科技有限公司 | 一种基于ⅱb型限制性内切酶酶切的简化基因组建库方法 |
| BR112017023257A2 (pt) * | 2015-04-29 | 2018-07-17 | Univ California | composições e métodos para construir bibliotecas de cdna de cadeias específicas |
| KR101858344B1 (ko) * | 2015-06-01 | 2018-05-16 | 연세대학교 산학협력단 | 바코드 서열을 포함하는 어댑터를 이용한 차세대 염기서열 분석 방법 |
| WO2017025564A1 (en) | 2015-08-10 | 2017-02-16 | Hs Diagnomics Gmbh | Method for providing tumour-specific t cells |
| CN105256379A (zh) * | 2015-11-23 | 2016-01-20 | 武汉大学 | 一种新的基因组简化甲基化测序文库的制备方法 |
| CN117512078A (zh) * | 2016-09-22 | 2024-02-06 | 西格马-奥尔德里奇有限责任公司 | 单引物至双引物扩增子转换 |
| US10633651B2 (en) * | 2017-07-10 | 2020-04-28 | Agilent Technologies, Inc. | Assay methods and compositions for detecting contamination of nucleic acid identifiers |
| JP7448974B2 (ja) | 2019-02-12 | 2024-03-13 | セリーセル ゲーエムベーハー | 逆免疫抑制 |
| IT201900024159A1 (it) * | 2019-12-16 | 2021-06-16 | Menarini Silicon Biosystems Spa | Metodo e kit per amplificazione totale del genoma e analisi di molecole bersaglio in un campione biologico |
| WO2022202728A1 (ja) * | 2021-03-26 | 2022-09-29 | 富士フイルム株式会社 | プライマー対、塩基配列変異の判定方法及び塩基配列の変異判定用キット |
| MX2024000731A (es) | 2021-07-15 | 2024-02-09 | Boehringer Ingelheim Int | Identificacion de receptores de celula t y antigenos especificos de tumor comunes. |
| JP2025518223A (ja) | 2022-05-30 | 2025-06-12 | ハーエス ダイアグノミクス ゲーエムベーハー | 共通の腫瘍特異的ｔ細胞受容体 |
| CN115961002B (zh) * | 2022-11-08 | 2025-11-25 | 浙江绍兴鼎晶生物医药科技股份有限公司 | 一种基于循环肿瘤dna的突变基因检测方法及其应用 |
| CN120548357A (zh) * | 2022-11-21 | 2025-08-26 | 百奥赛科生物科技有限公司 | 目标核酸序列的高通量扩增 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| WO1991015601A1 (en) * | 1990-04-05 | 1991-10-17 | The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce | Modified rna template-specific polymerase chain reaction |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| EP1498494A3 (en) | 1997-04-01 | 2007-06-20 | Solexa Ltd. | Method of nucleic acid sequencing |
| EP1591541B1 (en) | 1997-04-01 | 2012-02-15 | Illumina Cambridge Limited | Method of nucleic acid sequencing |
| WO1999020798A1 (en) * | 1997-10-23 | 1999-04-29 | Exact Laboratories, Inc. | Methods for detecting contamination in molecular diagnostics using pcr |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US8361712B2 (en) * | 2007-12-17 | 2013-01-29 | General Electric Company | Contamination-free reagents for nucleic acid amplification |
| US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| CA2513889A1 (en) | 2003-01-29 | 2004-08-19 | 454 Corporation | Double ended sequencing |
| US8748103B2 (en) * | 2008-11-07 | 2014-06-10 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
| EP2364368B1 (en) * | 2008-11-07 | 2014-01-15 | Sequenta, Inc. | Methods of monitoring conditions by sequence analysis |
| ES2960184T3 (es) * | 2010-06-09 | 2024-03-01 | Keygene Nv | Códigos de barras de secuencias combinatorias para el cribado de alto rendimiento |
-
2013
- 2013-07-04 EP EP13175199.2A patent/EP2746405B1/en active Active
- 2013-12-20 US US14/654,537 patent/US10894983B2/en active Active
- 2013-12-20 DK DK13814143.7T patent/DK2935618T3/en active
- 2013-12-20 CA CA2894635A patent/CA2894635C/en active Active
- 2013-12-20 JP JP2015548650A patent/JP6496665B2/ja active Active
- 2013-12-20 WO PCT/EP2013/077763 patent/WO2014096394A1/en not_active Ceased
- 2013-12-20 EP EP13814143.7A patent/EP2935618B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20150337368A1 (en) | 2015-11-26 |
| EP2746405A1 (en) | 2014-06-25 |
| JP2016500275A (ja) | 2016-01-12 |
| EP2935618B1 (en) | 2018-08-08 |
| WO2014096394A1 (en) | 2014-06-26 |
| JP6496665B2 (ja) | 2019-04-03 |
| CA2894635A1 (en) | 2014-06-26 |
| US10894983B2 (en) | 2021-01-19 |
| EP2746405B1 (en) | 2015-11-04 |
| DK2935618T3 (en) | 2018-12-03 |
| EP2935618A1 (en) | 2015-10-28 |
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