CA2894635C - Methods and primer sets for high throughput pcr sequencing - Google Patents

Methods and primer sets for high throughput pcr sequencing Download PDF

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Publication number
CA2894635C
CA2894635C CA2894635A CA2894635A CA2894635C CA 2894635 C CA2894635 C CA 2894635C CA 2894635 A CA2894635 A CA 2894635A CA 2894635 A CA2894635 A CA 2894635A CA 2894635 C CA2894635 C CA 2894635C
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sequence
seq
primers
primer
amplification
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French (fr)
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CA2894635A1 (en
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Steffen Hennig
Volkhard Seitz
Julia-Marie Ritter
Michael Hummel
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HS DIAGNOMICS GmbH
Therycell GmbH
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HS DIAGNOMICS GmbH
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2894635A 2012-12-23 2013-12-20 Methods and primer sets for high throughput pcr sequencing Active CA2894635C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP12199315 2012-12-23
EP12199315.8 2012-12-23
EP13175199.2A EP2746405B1 (en) 2012-12-23 2013-07-04 Methods and primer sets for high throughput PCR sequencing
EP13175199.2 2013-07-04
PCT/EP2013/077763 WO2014096394A1 (en) 2012-12-23 2013-12-20 Methods and primer sets for high throughput pcr sequencing

Publications (2)

Publication Number Publication Date
CA2894635A1 CA2894635A1 (en) 2014-06-26
CA2894635C true CA2894635C (en) 2021-03-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2894635A Active CA2894635C (en) 2012-12-23 2013-12-20 Methods and primer sets for high throughput pcr sequencing

Country Status (6)

Country Link
US (1) US10894983B2 (enExample)
EP (2) EP2746405B1 (enExample)
JP (1) JP6496665B2 (enExample)
CA (1) CA2894635C (enExample)
DK (1) DK2935618T3 (enExample)
WO (1) WO2014096394A1 (enExample)

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CN104562214A (zh) * 2014-12-26 2015-04-29 上海派森诺生物科技有限公司 一种基于ⅱb型限制性内切酶酶切的简化基因组建库方法
EP3289105B1 (en) 2015-04-29 2021-01-20 The Regents of The University of California Compositions and methods for constructing strand specific cdna libraries
KR101858344B1 (ko) * 2015-06-01 2018-05-16 연세대학교 산학협력단 바코드 서열을 포함하는 어댑터를 이용한 차세대 염기서열 분석 방법
EP3372683B1 (en) 2015-08-10 2020-05-27 HS Diagnomics GmbH Method for providing tumor-specific t cells and use thereof
CN105256379A (zh) * 2015-11-23 2016-01-20 武汉大学 一种新的基因组简化甲基化测序文库的制备方法
CN109804083B (zh) * 2016-09-22 2023-11-03 西格马-奥尔德里奇有限责任公司 单引物至双引物扩增子转换
US10633651B2 (en) * 2017-07-10 2020-04-28 Agilent Technologies, Inc. Assay methods and compositions for detecting contamination of nucleic acid identifiers
CA3127279A1 (en) 2019-02-12 2020-08-20 Therycell Gmbh Reverse immunosuppression
IT201900024159A1 (it) * 2019-12-16 2021-06-16 Menarini Silicon Biosystems Spa Metodo e kit per amplificazione totale del genoma e analisi di molecole bersaglio in un campione biologico
WO2022202728A1 (ja) * 2021-03-26 2022-09-29 富士フイルム株式会社 プライマー対、塩基配列変異の判定方法及び塩基配列の変異判定用キット
MX2024000731A (es) 2021-07-15 2024-02-09 Boehringer Ingelheim Int Identificacion de receptores de celula t y antigenos especificos de tumor comunes.
JP2025518223A (ja) 2022-05-30 2025-06-12 ハーエス ダイアグノミクス ゲーエムベーハー 共通の腫瘍特異的t細胞受容体
CN115961002B (zh) * 2022-11-08 2025-11-25 浙江绍兴鼎晶生物医药科技股份有限公司 一种基于循环肿瘤dna的突变基因检测方法及其应用
WO2024112758A1 (en) * 2022-11-21 2024-05-30 Biosearch Technologies, Inc. High-throughput amplification of targeted nucleic acid sequences

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
AU7653691A (en) * 1990-04-05 1991-10-30 United States of America, as represented by the Secretary, U.S. Department of Commerce, The Modified rna template-specific polymerase chain reaction
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
ATE364718T1 (de) 1997-04-01 2007-07-15 Solexa Ltd Verfahren zur vervielfältigung von nukleinsäure
EP1498494A3 (en) 1997-04-01 2007-06-20 Solexa Ltd. Method of nucleic acid sequencing
EP1025265A1 (en) * 1997-10-23 2000-08-09 Exact Laboratories, Inc. Methods for detecting contamination in molecular diagnostics using pcr
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US8361712B2 (en) * 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CA2513535C (en) 2003-01-29 2012-06-12 454 Corporation Bead emulsion nucleic acid amplification
US8748103B2 (en) * 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
GB2483810B (en) * 2008-11-07 2012-09-05 Sequenta Inc Methods for correlating clonotypes with diseases in a population
FI3425062T3 (fi) * 2010-06-09 2023-09-01 Keygene Nv Kombinatorisia sekvenssiviivakoodeja suuren suorituskyvyn seulontaa varten

Also Published As

Publication number Publication date
EP2746405A1 (en) 2014-06-25
JP2016500275A (ja) 2016-01-12
EP2935618A1 (en) 2015-10-28
US20150337368A1 (en) 2015-11-26
EP2935618B1 (en) 2018-08-08
EP2746405B1 (en) 2015-11-04
JP6496665B2 (ja) 2019-04-03
US10894983B2 (en) 2021-01-19
DK2935618T3 (en) 2018-12-03
CA2894635A1 (en) 2014-06-26
WO2014096394A1 (en) 2014-06-26

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