JP6496665B2 - ハイスループットｐｃｒシーケンシングのための方法及びプライマーのセット - Google Patents
ハイスループットｐｃｒシーケンシングのための方法及びプライマーのセット Download PDFInfo
- Publication number
- JP6496665B2 JP6496665B2 JP2015548650A JP2015548650A JP6496665B2 JP 6496665 B2 JP6496665 B2 JP 6496665B2 JP 2015548650 A JP2015548650 A JP 2015548650A JP 2015548650 A JP2015548650 A JP 2015548650A JP 6496665 B2 JP6496665 B2 JP 6496665B2
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- primer
- stage
- amplification
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12199315 | 2012-12-23 | ||
| EP12199315.8 | 2012-12-23 | ||
| EP13175199.2 | 2013-07-04 | ||
| EP13175199.2A EP2746405B1 (en) | 2012-12-23 | 2013-07-04 | Methods and primer sets for high throughput PCR sequencing |
| PCT/EP2013/077763 WO2014096394A1 (en) | 2012-12-23 | 2013-12-20 | Methods and primer sets for high throughput pcr sequencing |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016500275A JP2016500275A (ja) | 2016-01-12 |
| JP2016500275A5 JP2016500275A5 (enExample) | 2017-01-26 |
| JP6496665B2 true JP6496665B2 (ja) | 2019-04-03 |
Family
ID=47504728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015548650A Active JP6496665B2 (ja) | 2012-12-23 | 2013-12-20 | ハイスループットｐｃｒシーケンシングのための方法及びプライマーのセット |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10894983B2 (enExample) |
| EP (2) | EP2746405B1 (enExample) |
| JP (1) | JP6496665B2 (enExample) |
| CA (1) | CA2894635C (enExample) |
| DK (1) | DK2935618T3 (enExample) |
| WO (1) | WO2014096394A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104562214A (zh) * | 2014-12-26 | 2015-04-29 | 上海派森诺生物科技有限公司 | 一种基于ⅱb型限制性内切酶酶切的简化基因组建库方法 |
| EP3289105B1 (en) | 2015-04-29 | 2021-01-20 | The Regents of The University of California | Compositions and methods for constructing strand specific cdna libraries |
| WO2016195382A1 (ko) * | 2015-06-01 | 2016-12-08 | 연세대학교 산학협력단 | 바코드 서열을 포함하는 어댑터를 이용한 차세대 염기서열 분석 방법 |
| ES2679798T3 (es) | 2015-08-10 | 2018-08-31 | Hs Diagnomics Gmbh | Método para proporcionar linfocitos T específicos de tumor |
| CN105256379A (zh) * | 2015-11-23 | 2016-01-20 | 武汉大学 | 一种新的基因组简化甲基化测序文库的制备方法 |
| US11390913B2 (en) | 2016-09-22 | 2022-07-19 | Sigma-Aldrich Co. Llc | Single primer to dual primer amplicon switching |
| US10633651B2 (en) * | 2017-07-10 | 2020-04-28 | Agilent Technologies, Inc. | Assay methods and compositions for detecting contamination of nucleic acid identifiers |
| SG11202006110UA (en) * | 2018-01-05 | 2020-07-29 | Billiontoone Inc | Quality control templates for ensuring validity of sequencing-based assays |
| EP3923977A1 (en) | 2019-02-12 | 2021-12-22 | TheryCell GmbH | Reverse immunosuppression |
| IT201900024159A1 (it) * | 2019-12-16 | 2021-06-16 | Menarini Silicon Biosystems Spa | Metodo e kit per amplificazione totale del genoma e analisi di molecole bersaglio in un campione biologico |
| JPWO2022202728A1 (enExample) * | 2021-03-26 | 2022-09-29 | ||
| AU2022319819A1 (en) | 2021-07-15 | 2024-01-25 | Boehringer Ingelheim International Gmbh | Identification of common tumor-specific t cell receptors and antigens |
| EP4532536A1 (en) | 2022-05-30 | 2025-04-09 | HS Diagnomics GmbH | Common tumor-specific t cell receptors |
| CN115961002B (zh) * | 2022-11-08 | 2025-11-25 | 浙江绍兴鼎晶生物医药科技股份有限公司 | 一种基于循环肿瘤dna的突变基因检测方法及其应用 |
| CN120548357A (zh) * | 2022-11-21 | 2025-08-26 | 百奥赛科生物科技有限公司 | 目标核酸序列的高通量扩增 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| AU7653691A (en) * | 1990-04-05 | 1991-10-30 | United States of America, as represented by the Secretary, U.S. Department of Commerce, The | Modified rna template-specific polymerase chain reaction |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| EP2327797B1 (en) | 1997-04-01 | 2015-11-25 | Illumina Cambridge Limited | Method of nucleic acid sequencing |
| JP2001517948A (ja) | 1997-04-01 | 2001-10-09 | グラクソ、グループ、リミテッド | 核酸配列決定法 |
| CA2307177C (en) * | 1997-10-23 | 2004-06-29 | Exact Laboratories, Inc. | Methods for detecting contamination in molecular diagnostics using pcr |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US8361712B2 (en) * | 2007-12-17 | 2013-01-29 | General Electric Company | Contamination-free reagents for nucleic acid amplification |
| US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| DE602004026033D1 (de) | 2003-01-29 | 2010-04-29 | 454 Corp | Zweiendige sequenzierung |
| US8748103B2 (en) * | 2008-11-07 | 2014-06-10 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
| EP2364368B1 (en) * | 2008-11-07 | 2014-01-15 | Sequenta, Inc. | Methods of monitoring conditions by sequence analysis |
| EP2580351B1 (en) * | 2010-06-09 | 2018-08-29 | Keygene N.V. | Combinatorial sequence barcodes for high throughput screening |
-
2013
- 2013-07-04 EP EP13175199.2A patent/EP2746405B1/en active Active
- 2013-12-20 EP EP13814143.7A patent/EP2935618B1/en active Active
- 2013-12-20 DK DK13814143.7T patent/DK2935618T3/en active
- 2013-12-20 JP JP2015548650A patent/JP6496665B2/ja active Active
- 2013-12-20 US US14/654,537 patent/US10894983B2/en active Active
- 2013-12-20 CA CA2894635A patent/CA2894635C/en active Active
- 2013-12-20 WO PCT/EP2013/077763 patent/WO2014096394A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2014096394A1 (en) | 2014-06-26 |
| EP2935618A1 (en) | 2015-10-28 |
| US20150337368A1 (en) | 2015-11-26 |
| EP2935618B1 (en) | 2018-08-08 |
| US10894983B2 (en) | 2021-01-19 |
| EP2746405A1 (en) | 2014-06-25 |
| CA2894635A1 (en) | 2014-06-26 |
| JP2016500275A (ja) | 2016-01-12 |
| DK2935618T3 (en) | 2018-12-03 |
| CA2894635C (en) | 2021-03-02 |
| EP2746405B1 (en) | 2015-11-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2016500275A (ja) | ハイスループットｐｃｒシーケンシングのための方法及びプライマーのセット | |
| CN110191961B (zh) | 制备经不对称标签化的测序文库的方法 | |
| JP6327652B2 (ja) | 分子計数による対立遺伝子呼び出しの信頼度の増加 | |
| US20200139335A1 (en) | Enrichment of DNA Sequencing Libraries from Samples Containing Small Amounts of Target DNA | |
| JP6525473B2 (ja) | 複製物配列決定リードを同定するための組成物および方法 | |
| JP2021168659A (ja) | 次世代ゲノムウォーキングのための方法ならびに関連する組成物およびキット | |
| JP6664575B2 (ja) | 核酸分子数計測法 | |
| CN105026577A (zh) | 通过序列捕获检测基因组重排 | |
| WO2012042374A2 (en) | Method of determining number or concentration of molecules | |
| EP4105341B1 (en) | A primer for next generation sequencer and a method for producing the same, a dna library obtained through the use of a primer for next generation sequencer and a method for producing the same, and a dna analyzing method using a dna library | |
| CN113710815B (zh) | 用于多重拷贝数变异检测和等位基因比率定量的定量扩增子测序 | |
| JP2018527928A (ja) | 次世代シークエンシングのための高分子量ｄｎａサンプル追跡タグ | |
| JP2016500275A5 (enExample) | ||
| JP6908615B2 (ja) | ヌクレアーゼ保護を使用する直接標的シーケンシングの方法 | |
| JP2021514205A5 (enExample) | ||
| EP3625371B1 (en) | Set of random primers and method for preparing dna library using the same | |
| JP7152599B2 (ja) | 塩基配列決定のためのモジュール式およびコンビナトリアル核酸試料調製のためのシステムおよび方法 | |
| US20210130815A1 (en) | Evaluating Genomic Variation Using Repetitive Nucleic Acid Sequences | |
| KR101484259B1 (ko) | 남조류의 유전자 증폭용 프라이머 및 이를 이용한 남조류의 탐지방법 | |
| IT202300016836A1 (it) | Procedimento e kit per diagnosi, prognosi, monitoraggio di terapia e valutazione della previsione di esito di una malattia | |
| HK40018808B (en) | Probe set for analyzing a dna sample and method for using the same | |
| Mukherjee et al. | Advances in PCR based molecular markers and its application in biodiversity conservation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20161209 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20161209 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20171226 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20180322 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20180525 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180621 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20181106 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190123 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20190305 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20190311 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6496665 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| S111 | Request for change of ownership or part of ownership |
Free format text: JAPANESE INTERMEDIATE CODE: R313114 |
|
| S531 | Written request for registration of change of domicile |
Free format text: JAPANESE INTERMEDIATE CODE: R313531 |
|
| R350 | Written notification of registration of transfer |
Free format text: JAPANESE INTERMEDIATE CODE: R350 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |