CA2892533A1 - Methods and compositions for producing and selecting transgenic plants - Google Patents

Methods and compositions for producing and selecting transgenic plants Download PDF

Info

Publication number
CA2892533A1
CA2892533A1 CA2892533A CA2892533A CA2892533A1 CA 2892533 A1 CA2892533 A1 CA 2892533A1 CA 2892533 A CA2892533 A CA 2892533A CA 2892533 A CA2892533 A CA 2892533A CA 2892533 A1 CA2892533 A1 CA 2892533A1
Authority
CA
Canada
Prior art keywords
plant
promoter
polynucleotide
polypeptide
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2892533A
Other languages
English (en)
French (fr)
Inventor
Myeong-Je Cho
Samuel R. ELLIS
William J. Gordon-Kamm
Zuo-Yu Zhao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Pioneer Hi Bred International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred International Inc filed Critical Pioneer Hi Bred International Inc
Publication of CA2892533A1 publication Critical patent/CA2892533A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/30—Phosphinic acids [R2P(=O)(OH)]; Thiophosphinic acids ; [R2P(=X1)(X2H) (X1, X2 are each independently O, S or Se)]
    • C07F9/301—Acyclic saturated acids which can have further substituents on alkyl
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
    • C07F9/3804—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)] not used, see subgroups
    • C07F9/3808—Acyclic saturated acids which can have further substituents on alkyl
    • C07F9/3813—N-Phosphonomethylglycine; Salts or complexes thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Catalysts (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)
CA2892533A 2012-12-13 2013-12-11 Methods and compositions for producing and selecting transgenic plants Abandoned CA2892533A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261736947P 2012-12-13 2012-12-13
US61/736,947 2012-12-13
US13/800,447 2013-03-13
US13/800,447 US20140173775A1 (en) 2012-12-13 2013-03-13 Methods and compositions for producing and selecting transgenic plants
PCT/US2013/074379 WO2014093485A1 (en) 2012-12-13 2013-12-11 Methods and compositions for producing and selecting transgenic plants

Publications (1)

Publication Number Publication Date
CA2892533A1 true CA2892533A1 (en) 2014-06-19

Family

ID=50932653

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2892533A Abandoned CA2892533A1 (en) 2012-12-13 2013-12-11 Methods and compositions for producing and selecting transgenic plants

Country Status (10)

Country Link
US (2) US20140173781A1 (enExample)
EP (1) EP2931900A1 (enExample)
JP (1) JP6383778B2 (enExample)
CN (1) CN104968790A (enExample)
AU (1) AU2013359340A1 (enExample)
CA (1) CA2892533A1 (enExample)
IL (2) IL238608A0 (enExample)
RU (1) RU2015127989A (enExample)
SA (1) SA515361041B1 (enExample)
WO (3) WO2014093485A1 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2742380T3 (es) * 2012-06-26 2020-02-14 Uop Llc Proceso de alquilación usando líquidos iónicos a base de fosfonio
BR112018008705B1 (pt) * 2015-10-30 2023-12-26 Pioneer Hi-Bred International, Inc Método para produzir uma planta transgênica
EP3550958A4 (en) * 2016-12-08 2020-10-07 Syngenta Participations AG PROCESSES FOR IMPROVING THE FREQUENCY OF TRANSFORMATION
US11180770B2 (en) 2017-03-07 2021-11-23 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
US11371056B2 (en) 2017-03-07 2022-06-28 BASF Agricultural Solutions Seed US LLC HPPD variants and methods of use
EP3601578A1 (en) * 2017-03-23 2020-02-05 Basf Se Anther-specific promoter and uses thereof
AU2018243654B2 (en) 2017-03-31 2024-07-25 Pioneer Hi-Bred International, Inc. Expression modulating elements and use thereof
CA3092073A1 (en) 2018-03-12 2019-09-19 Pioneer Hi-Bred International, Inc. Use of morphogenic factors for the improvement of gene editing
WO2020005588A1 (en) * 2018-06-29 2020-01-02 BASF Agricultural Solutions Seed US LLC Herbicide tolerant plants expressing a cyanobacterial plastoquinone biosynthetic pathway
CN109212053B (zh) * 2018-07-07 2021-05-14 丁立平 一种测定饮用水中痕量草铵膦、草甘膦及氨甲基膦酸的气相色谱法
CN109666757B (zh) * 2019-01-31 2022-04-19 宁夏农林科学院农作物研究所（宁夏回族自治区农作物育种中心） 用于鉴定小麦春化基因vrn-d4的试剂盒及其专用成套引物对
JP7743308B2 (ja) * 2019-03-27 2025-09-24 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド 植物外稙片の形質転換
CN110106200B (zh) * 2019-05-17 2021-02-02 中国农业科学院作物科学研究所 玉米bbm1基因在提高植物遗传转化效率中的应用
US20220356481A1 (en) * 2019-08-12 2022-11-10 Syngenta Crop Protection Ag Wox genes
CN110950943B (zh) * 2019-12-30 2021-09-17 上海交通大学 紫花苜蓿‘WL525’胚胎发育晚期蛋白MsLEA-D34及其编码基因
EP4268583A4 (en) * 2020-12-28 2025-01-08 Kaneka Corporation Method for producing transformed cells of corn or corn plant, nucleic acid construct, and method for introducing nucleic acid to cells or corn plant
US20250154522A1 (en) * 2021-11-02 2025-05-15 University Of Florida Research Foundation, Incorporated Improved genetic transformation by improving de-novo shoot regeneration in adult plants and during in-vitro tissue culture
CN118109503B (zh) * 2024-04-16 2025-04-25 福建农林大学 大豆生长素酰胺合成酶gh3基因家族及其应用

Family Cites Families (132)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US762264A (en) 1903-11-06 1904-06-07 Pierre C Waring Process of making felt cloth.
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5304732A (en) 1984-03-06 1994-04-19 Mgi Pharma, Inc. Herbicide resistance in plants
US5331107A (en) 1984-03-06 1994-07-19 Mgi Pharma, Inc. Herbicide resistance in plants
US4761373A (en) 1984-03-06 1988-08-02 Molecular Genetics, Inc. Herbicide resistance in plants
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
ATE57390T1 (de) 1986-03-11 1990-10-15 Plant Genetic Systems Nv Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen.
US5273894A (en) 1986-08-23 1993-12-28 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5605011A (en) 1986-08-26 1997-02-25 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5378824A (en) 1986-08-26 1995-01-03 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5013659A (en) 1987-07-27 1991-05-07 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US4764440A (en) 1987-05-05 1988-08-16 Eveready Battery Company Low temperature molten compositions
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
JPH01258408A (ja) * 1988-04-08 1989-10-16 Asahi Glass Co Ltd 非水電解液エネルギー貯蔵装置
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
DE69033628T2 (de) 1989-10-31 2001-04-19 Monsanto Co., St. Louis Promotor für transgene Pflanzen
FR2659871B1 (fr) 1990-03-20 1992-06-05 Inst Francais Du Petrole Composition liquide non aqueuse a caractere ionique et son utilisation comme solvant. invention de mm. yves chauvin, dominique commereuc, isabelle guibard, andre hirschauer, helene olivier, lucien saussine.
DE69133128T2 (de) 1990-04-12 2003-06-18 Syngenta Participations Ag, Basel Gewebe-spezifische Promotoren
EP0528857B1 (en) 1990-04-26 2002-01-30 Aventis CropScience N.V. New bacillus thuringiensis strain and its gene encoding insecticidal toxin
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
DE69132939T2 (de) 1990-06-25 2002-11-14 Monsanto Technology Llc Glyphosattolerante pflanzen
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5277905A (en) 1991-01-16 1994-01-11 Mycogen Corporation Coleopteran-active bacillus thuringiensis isolate
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
US5731180A (en) 1991-07-31 1998-03-24 American Cyanamid Company Imidazolinone resistant AHAS mutants
ES2124738T3 (es) 1991-08-02 1999-02-16 Mycogen Corp Nuevo microorganismo e insecticida.
HU219057B (hu) 1991-08-27 2001-02-28 Novartis Ag. Inszekticid hatású proteinek Homoptera rovarok ellen és alkalmazásuk a növényvédelemben
EP0612208B1 (en) 1991-10-04 2004-09-15 North Carolina State University Pathogen-resistant transgenic plants
TW261517B (enExample) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5428148A (en) 1992-04-24 1995-06-27 Beckman Instruments, Inc. N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
CA2140910C (en) 1992-07-27 1999-03-23 Jeffrey A. Townsend An improved method of agrobacterium-mediated transformation of cultured soybean cells
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
ATE205533T1 (de) 1993-01-13 2001-09-15 Pioneer Hi Bred Int Derivate von alpha-hordothionin mit höherem behalt an lysin
US5583210A (en) 1993-03-18 1996-12-10 Pioneer Hi-Bred International, Inc. Methods and compositions for controlling plant development
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5593881A (en) 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5767373A (en) 1994-06-16 1998-06-16 Novartis Finance Corporation Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
CZ331797A3 (cs) 1995-04-20 1998-06-17 American Cyanamid Company Produkty rezistentní na herbicidy vyvíjené na struktuře založeným způsobem
US5853973A (en) 1995-04-20 1998-12-29 American Cyanamid Company Structure based designed herbicide resistant products
PL323635A1 (en) 1995-06-02 1998-04-14 Pioneer Hi Bred Int Derivatives of alpha-hordothionine of high methionine content
AU705933B2 (en) 1995-06-02 1999-06-03 Pioneer Hi-Bred International, Inc. High threonine derivatives of alpha-hordothionin
FR2734842B1 (fr) 1995-06-02 1998-02-27 Rhone Poulenc Agrochimie Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides
DE69623057T2 (de) 1995-06-07 2003-03-27 Invitrogen Corp., Carlsbad Rekombinatorische klonierung in vitro unter verwendung genmanipulierter rekombinationsorte
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US5737514A (en) 1995-11-29 1998-04-07 Texas Micro, Inc. Remote checkpoint memory system and protocol for fault-tolerant computer system
US5703049A (en) 1996-02-29 1997-12-30 Pioneer Hi-Bred Int'l, Inc. High methionine derivatives of α-hordothionin for pathogen-control
AUPN903196A0 (en) * 1996-03-29 1996-04-26 Australian National University, The Single-step excision means
US6175056B1 (en) 1996-04-29 2001-01-16 The Procter & Gamble Company Disposable absorbent article having an expanding topsheet and being capable of self-shaping in use
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
US5824832A (en) 1996-07-22 1998-10-20 Akzo Nobel Nv Linear alxylbenzene formation using low temperature ionic liquid
JP3441899B2 (ja) 1996-11-01 2003-09-02 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
DE19652284A1 (de) 1996-12-16 1998-06-18 Hoechst Schering Agrevo Gmbh Neue Gene codierend für Aminosäure-Deacetylasen mit Spezifität für N-Acetyl-L-Phosphinothricin, ihre Isolierung und Verwendung
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
AU7968098A (en) 1997-06-11 1998-12-30 United States Of America, As Represented By The Secretary Of Agriculture, The Transformation of wheat with the cyanamide hydratase gene
CA2302471A1 (en) * 1997-09-05 1999-03-11 Purdue Research Foundation Selective expression of genes in plants
US6245968B1 (en) 1997-11-07 2001-06-12 Aventis Cropscience S.A. Mutated hydroxyphenylpyruvate dioxygenase, DNA sequence and isolation of plants which contain such a gene and which are tolerant to herbicides
US6069115A (en) 1997-11-12 2000-05-30 Rhone-Poulenc Agrochimie Method of controlling weeds in transgenic crops
JP4206154B2 (ja) 1997-11-13 2009-01-07 大日本住友製薬株式会社 変異型ｌｏｘＰ配列とその応用
PT1032680E (pt) 1997-11-18 2006-05-31 Pioneer Hi Bred Int Um novo metodo para a integracao de adn estranho em genomas eucarioticos
EP1034262B1 (en) 1997-11-18 2005-08-17 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
NZ504510A (en) 1997-11-18 2002-10-25 Pioneer Hi Bred Int Methods and compositions for increasing efficiency of excision of a viral replicon from T-DNA that is transferred to a plant by agroinfection
EP1032692A1 (en) 1997-11-18 2000-09-06 Pioneer Hi-Bred International, Inc. Targeted manipulation of herbicide-resistance genes in plants
US5929301A (en) 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase
EP1056862A1 (en) 1998-02-26 2000-12-06 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
EP1056864B1 (en) 1998-02-26 2004-10-06 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
US6070283A (en) 1998-07-10 2000-06-06 Rite-Hite Holding Corporation Dock leveler with integral bumpers
ES2252964T3 (es) 1998-08-20 2006-05-16 Pioneer Hi-Bred International, Inc. Promotores preferidos de semillas.
AU5788299A (en) 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from (end) genes
IL142736A0 (en) 1998-11-09 2002-03-10 Pioneer Hi Bred Int Transcriptional activator lec 1 nucleic acids, polypeptides and their uses
US6890726B1 (en) 1999-04-06 2005-05-10 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
JP4808880B2 (ja) 1999-07-23 2011-11-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア バクテリオファージｐｈｉｃ３１組み換え系による真核細胞におけるｄｎａ組み換え
WO2001011058A1 (en) 1999-08-09 2001-02-15 Monsanto Technology Llc Novel cloning methods and vectors
WO2001012825A1 (en) 1999-08-13 2001-02-22 Syngenta Participations Ag Herbicide-tolerant protoporphyrinogen oxidase
US7060499B1 (en) 1999-09-30 2006-06-13 Izumu Saito DNA containing variant FRT sequences
US7256322B2 (en) 1999-10-01 2007-08-14 Pioneer Hi-Bred International, Inc. Wuschel (WUS) Gene Homologs
DE60034929T2 (de) 1999-10-01 2008-01-24 E.I. Du Pont De Nemours And Co., Wilmington Wuschel (WUS)-Proteine
ATE412758T1 (de) 2000-06-28 2008-11-15 Sungene Gmbh & Co Kgaa Binärvektoren zur verbesserten transformation von pflanzlichen systemen
US6512165B1 (en) 2000-07-10 2003-01-28 Pioneer Hi-Bred International, Inc. Methods for enhancing plant transformation frequencies
US7462481B2 (en) 2000-10-30 2008-12-09 Verdia, Inc. Glyphosate N-acetyltransferase (GAT) genes
CZ20031120A3 (cs) 2000-10-30 2003-11-12 Maxygen, Inc. Nové geny glyfosát-N-acetyltransferázy (GAT)
US7294759B2 (en) 2001-06-29 2007-11-13 E. I. Du Pont De Nemours And Company Alteration of oil traits in plants
AT5356U1 (de) 2001-07-16 2002-06-25 Uv & P Umweltman Verfahrenstec Verfahren zur immobilisierung von verbrennungsrückständen
AU2002360986A1 (en) 2001-12-20 2003-07-09 Sungene Gmbh And Co. Kgaa Methods for the transformation of vegetal plastids
CA2422480A1 (en) 2002-03-15 2003-09-15 National Research Council Of Canada Promoters for molecular farming and/or early detection of transgenic plants
US7238854B2 (en) 2002-04-11 2007-07-03 E. I. Du Pont De Nemours And Company Method of controlling site-specific recombination
AR039501A1 (es) 2002-04-30 2005-02-23 Verdia Inc Genes de glifosato n-acetil transferasa (gat)
US20040203141A1 (en) 2003-04-11 2004-10-14 Jorge Dubcovsky Use of the AP1 gene promoter to control the vernalization response and flowering time in grasses
CN1863914B (zh) 2003-04-29 2011-03-09 先锋高级育种国际公司 新的草甘膦-n-乙酰转移酶(gat)基因
US8329603B2 (en) * 2003-09-16 2012-12-11 Uop Llc Isoparaffin-olefin alkylation
US7579529B2 (en) 2004-02-02 2009-08-25 Pioneer Hi-Bred International, Inc. AP2 domain transcription factor ODP2 (ovule development protein 2) and methods of use
AR048710A1 (es) 2004-04-26 2006-05-17 Pioneer Hi Bred Int Activadores de la transcripcion que participan en la tolerancia al estres abiotico
US7405074B2 (en) 2004-04-29 2008-07-29 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GAT) genes
US7473822B1 (en) 2005-06-27 2009-01-06 Iowa State University Research Foundation, Inc. Soybean transformation and regeneration using half-seed explant
WO2007011733A2 (en) 2005-07-18 2007-01-25 Pioneer Hi-Bred International, Inc. Modified frt recombination sites and methods of use
BRPI0615087A2 (pt) 2005-08-24 2011-05-03 Pioneer Hi Bred Int métodos e composições para a expressão de um polinucleotìdeo de interesse
US7491813B2 (en) 2005-12-07 2009-02-17 Monsanto Technology Llc Promoter polynucleotides identified from Zea mays for use in plants
US7390928B2 (en) * 2005-12-14 2008-06-24 Chemtura Corporation Alkylation of N′-phenyl-N-alkylphenylenediamines in ionic liquid, N′-phenyl-N-alkyl (alkylphenylene) diamines produced thereby, and lubricant compositions containing same
US7569740B2 (en) * 2005-12-20 2009-08-04 Chevron U.S.A. Inc. Alkylation of olefins with isoparaffins in ionic liquid to make lubricant or fuel blendstock
US7928296B2 (en) 2006-10-30 2011-04-19 Pioneer Hi-Bred International, Inc. Maize event DP-098140-6 and compositions and methods for the identification and/or detection thereof
GB2447416B (en) * 2006-12-15 2010-08-25 Uni I Stavanger Methods and vectors for transformation of plastids and marker excision using site-specific recombination
US8710206B2 (en) 2007-06-15 2014-04-29 E. I. Du Pont De Nemours And Company Soybean EF1A promoter and its use in constitutive expression of transgenic genes in plants
CN101284822A (zh) * 2008-06-06 2008-10-15 福建师范大学 一种制备环氧脂肪酸二元醇酯的方法
GB0816880D0 (en) * 2008-09-15 2008-10-22 Syngenta Ltd Improvements in or relating to organic compounds
MX2011003766A (es) 2008-10-28 2011-04-27 Pioneer Hi Bred Int Proteinas represoras responsivas a sulfonilurea.
AU2010239155B2 (en) * 2009-04-24 2016-03-03 Australian Centre For Plant Functional Genomics Pty Ltd Drought responsive expression of genes from the Zea mays Rab17 promoter
US8581046B2 (en) 2010-11-24 2013-11-12 Pioneer Hi-Bred International, Inc. Brassica gat event DP-073496-4 and compositions and methods for the identification and/or detection thereof
WO2011082318A2 (en) * 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants
US20120136189A1 (en) * 2010-03-09 2012-05-31 Conocophillips Company - Ip Services Group Buffered ionic liquids for olefin dimerization
CN102905517A (zh) * 2010-04-23 2013-01-30 先锋国际良种公司 基因开关组合物及使用方法
CN101979143B (zh) * 2010-09-15 2012-07-04 湖南科茂林化有限公司 一种松香或其衍生物的酯化催化剂及其应用
DK3336962T3 (en) * 2011-06-17 2019-04-01 Nantenergy Inc ELECTROCHEMICAL CELL COMPREHENSIVE ELECTROLYT WITH A HETERO-IONIC COMPLEX
BR112014004812A2 (pt) * 2011-08-31 2018-10-23 Du Pont métodos para regenerar uma planta e para produzir uma planta transformada
ES2742380T3 (es) * 2012-06-26 2020-02-14 Uop Llc Proceso de alquilación usando líquidos iónicos a base de fosfonio

Also Published As

Publication number Publication date
RU2015127989A (ru) 2017-01-19
JP6383778B2 (ja) 2018-08-29
AU2013359340A1 (en) 2015-04-09
US20140173775A1 (en) 2014-06-19
JP2016512496A (ja) 2016-04-28
WO2014093485A1 (en) 2014-06-19
CN104968790A (zh) 2015-10-07
EP2931900A1 (en) 2015-10-21
IL238821A0 (en) 2015-06-30
IL238608A0 (en) 2015-06-30
SA515361041B1 (ar) 2018-07-08
US20140173781A1 (en) 2014-06-19
WO2014143304A1 (en) 2014-09-18
WO2014143324A1 (en) 2014-09-18

Similar Documents

Publication Publication Date Title
US20140173775A1 (en) Methods and compositions for producing and selecting transgenic plants
US12173294B2 (en) Generation of site specific integration sites for complex trait loci in corn and soybean, and methods of use
US11512321B2 (en) Methods and compositions for the introduction and regulated expression of genes in plants
US12146144B2 (en) Methods and compositions for generating complex trait loci
CN102348802B (zh) 突变体羟基苯丙酮酸双加氧酶多肽及其使用方法
US20100154083A1 (en) Methods and Compositions for the Selection of A Transgenic Plant
EP3270685A1 (en) Methods and compositions for accelerated trait introgression
WO2019090017A1 (en) Genes and gene combinations for enhanced crops
US20250311689A1 (en) Seedling germination and growth conditions
WO2023073383A1 (en) Error prone dna polymerase for organelle mutation
AU2013381386A1 (en) Methods and compositions for producing and selecting transgenic plants
EP3947696A1 (en) Modified agrobacterium strains and use thereof for plant transformation
US12497628B2 (en) Methods and compositions for the introduction and regulated expression of genes in plants
US20070220627A1 (en) Methods and Compositions for the Selection of a Transgenic Plant

Legal Events

Date Code Title Description
FZDE Discontinued

Effective date: 20171212