CA2888810C - Pens for biological micro-objects - Google Patents
Pens for biological micro-objects Download PDFInfo
- Publication number
- CA2888810C CA2888810C CA2888810A CA2888810A CA2888810C CA 2888810 C CA2888810 C CA 2888810C CA 2888810 A CA2888810 A CA 2888810A CA 2888810 A CA2888810 A CA 2888810A CA 2888810 C CA2888810 C CA 2888810C
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- micro
- pens
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- pen
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- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 description 1
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- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/005—Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C5/00—Separating dispersed particles from liquids by electrostatic effect
- B03C5/02—Separators
- B03C5/022—Non-uniform field separators
- B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0424—Dielectrophoretic forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/084—Passive control of flow resistance
- B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Fluid Mechanics (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Food Science & Technology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261720956P | 2012-10-31 | 2012-10-31 | |
| US61/720,956 | 2012-10-31 | ||
| US14/060,117 US9857333B2 (en) | 2012-10-31 | 2013-10-22 | Pens for biological micro-objects |
| US14/060,117 | 2013-10-22 | ||
| PCT/US2013/067479 WO2014070873A1 (en) | 2012-10-31 | 2013-10-30 | Pens for biological micro-objects |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2888810A1 CA2888810A1 (en) | 2014-05-08 |
| CA2888810C true CA2888810C (en) | 2023-04-04 |
Family
ID=50545993
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2888810A Active CA2888810C (en) | 2012-10-31 | 2013-10-30 | Pens for biological micro-objects |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US9857333B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP2914965B1 (cg-RX-API-DMAC7.html) |
| JP (3) | JP6529908B2 (cg-RX-API-DMAC7.html) |
| KR (3) | KR101998918B1 (cg-RX-API-DMAC7.html) |
| CN (2) | CN108070582B (cg-RX-API-DMAC7.html) |
| AU (3) | AU2013337937B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2888810C (cg-RX-API-DMAC7.html) |
| DK (1) | DK2914965T3 (cg-RX-API-DMAC7.html) |
| IL (2) | IL272535B2 (cg-RX-API-DMAC7.html) |
| SG (2) | SG10201606540RA (cg-RX-API-DMAC7.html) |
| WO (1) | WO2014070873A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (78)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2014340089B2 (en) * | 2013-10-22 | 2019-09-26 | Berkeley Lights, Inc. | Microfluidic devices having isolation pens and methods of testing biological micro-objects with same |
| KR102113990B1 (ko) | 2013-10-22 | 2020-05-25 | 버클리 라잇츠, 인크. | 생물학적 활성물 분석용 미세 유체 소자 |
| US9889445B2 (en) * | 2013-10-22 | 2018-02-13 | Berkeley Lights, Inc. | Micro-fluidic devices for assaying biological activity |
| CN110437975B (zh) * | 2013-10-22 | 2023-06-09 | 伯克利之光生命科技公司 | 具有隔离围栏的微流体装置及用它测试生物微目标的方法 |
| US20150166326A1 (en) | 2013-12-18 | 2015-06-18 | Berkeley Lights, Inc. | Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device |
| US20150306599A1 (en) | 2014-04-25 | 2015-10-29 | Berkeley Lights, Inc. | Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus |
| US20150306598A1 (en) * | 2014-04-25 | 2015-10-29 | Berkeley Lights, Inc. | DEP Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus |
| US11192107B2 (en) | 2014-04-25 | 2021-12-07 | Berkeley Lights, Inc. | DEP force control and electrowetting control in different sections of the same microfluidic apparatus |
| US20150352547A1 (en) | 2014-06-06 | 2015-12-10 | Berkeley Lights, Inc. | Isolating Microfluidic Structures and Trapping Bubbles |
| EP3227025B1 (en) | 2014-12-05 | 2019-03-27 | The Regents of The University of California | Single-sided light-actuated microfluidic device with integrated mesh ground |
| US10058865B2 (en) | 2014-12-08 | 2018-08-28 | Berkeley Lights, Inc. | Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof |
| KR102369770B1 (ko) | 2014-12-08 | 2022-03-02 | 버클리 라잇츠, 인크. | 측방향/수직 트랜지스터 구조들을 포함하는 미세유체 디바이스 및 그 제조 및 사용 프로세스 |
| CN107223208B (zh) | 2014-12-09 | 2021-04-09 | 伯克利之光生命科技公司 | 微流体装置中微物体的自动检测和重新定位 |
| EP3230718B1 (en) * | 2014-12-09 | 2022-03-02 | Berkeley Lights, Inc. | Automated detection of assay-positive areas or of analyte quantities in microfluidic devices |
| TWI700125B (zh) * | 2014-12-10 | 2020-08-01 | 美商柏克萊燈光有限公司 | 用於操作電氣動力裝置之系統 |
| WO2016094715A2 (en) | 2014-12-10 | 2016-06-16 | Berkeley Lights, Inc. | Movement and selection of micro-objects in a microfluidic apparatus |
| CN107427315A (zh) * | 2015-03-04 | 2017-12-01 | 伯克利照明有限公司 | 体外胚胎的生殖和选择 |
| AU2016226008A1 (en) * | 2015-03-04 | 2017-10-26 | Berkeley Lights, Inc. | Generation and selection of embryos in vitro |
| US10751715B1 (en) | 2015-04-22 | 2020-08-25 | Berkeley Lights, Inc. | Microfluidic reporter cell assay methods and kits thereof |
| CN107849505B (zh) * | 2015-04-22 | 2021-09-07 | 伯克利之光生命科技公司 | 微流体细胞培养 |
| WO2016172623A1 (en) * | 2015-04-22 | 2016-10-27 | Berkeley Lights, Inc. | Manipulation of cell nuclei in a micro-fluidic device |
| JP2018512875A (ja) * | 2015-04-22 | 2018-05-24 | バークレー ライツ，インコーポレイテッド | マイクロ流体デバイスにおける細胞の凍結及び保管 |
| CA3176018A1 (en) | 2015-04-22 | 2016-10-27 | Berkeley Lights, Inc. | Microfluidic device for culturing biological cells and methods of use thereof |
| WO2017075295A1 (en) * | 2015-10-27 | 2017-05-04 | Berkeley Lights, Inc. | Microfluidic electrowetting device apparatus having a covalently bound hydrophobic surface |
| US10799865B2 (en) | 2015-10-27 | 2020-10-13 | Berkeley Lights, Inc. | Microfluidic apparatus having an optimized electrowetting surface and related systems and methods |
| IL299366A (en) * | 2015-11-23 | 2023-02-01 | Berkeley Lights Inc | In situ-generated microfluidic isolation structures, kits and methods of use thereof |
| US9988624B2 (en) | 2015-12-07 | 2018-06-05 | Zymergen Inc. | Microbial strain improvement by a HTP genomic engineering platform |
| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
| CN108603878A (zh) | 2015-12-08 | 2018-09-28 | 伯克利之光生命科技公司 | 微流体设备和试剂盒及其使用方法 |
| IL299835A (en) | 2015-12-30 | 2023-03-01 | Berkeley Lights Inc | Microfluidic devices for optically activated convection and displacement, and their kits and methods |
| TWI808934B (zh) | 2015-12-31 | 2023-07-21 | 美商伯克利之光生命科技公司 | 經工程化以表現促發炎多肽之腫瘤浸潤細胞 |
| TWI821913B (zh) | 2016-01-15 | 2023-11-11 | 美商伯克利之光生命科技公司 | 製造患者專一性抗癌治療劑之方法及使用該治療劑之治療方法 |
| WO2017160991A1 (en) | 2016-03-16 | 2017-09-21 | Lavieu Gregory G | Methods, systems and devices for selection and generation of genome edited clones |
| AU2017234787B2 (en) * | 2016-03-17 | 2022-12-22 | Berkeley Lights, Inc. | Selection and cloning of T lymphocytes in a microfluidic device |
| CN109311930B (zh) | 2016-03-31 | 2023-02-24 | 伯克利之光生命科技公司 | 核酸稳定试剂、试剂盒及其使用方法 |
| CN115184315A (zh) | 2016-04-15 | 2022-10-14 | 伯克利之光生命科技公司 | 用于坞内测定的方法、系统和试剂盒 |
| US10675625B2 (en) | 2016-04-15 | 2020-06-09 | Berkeley Lights, Inc | Light sequencing and patterns for dielectrophoretic transport |
| WO2017205830A1 (en) | 2016-05-26 | 2017-11-30 | Berkeley Lights, Inc. | Covalently modified surfaces, kits, and methods of preparation and use |
| ES2899107T3 (es) * | 2016-06-21 | 2022-03-10 | Quantumdx Group Ltd | Concentración de células diana mejorada mediante dielectroforesis (DEP) |
| KR102456488B1 (ko) | 2016-07-21 | 2022-10-19 | 버클리 라잇츠, 인크. | 미세유체 디바이스에서의 t 림프구들의 분류 |
| JP7280181B2 (ja) | 2016-10-01 | 2023-05-23 | バークレー ライツ，インコーポレイテッド | Ｄｎａバーコード組成物及びマイクロ流体デバイスによるインサイチュ同定法 |
| CN110248733B (zh) | 2016-12-01 | 2022-01-11 | 伯克利之光生命科技公司 | 用于对微物体成像的设备、系统和方法 |
| CA3045333A1 (en) | 2016-12-01 | 2018-06-07 | Berkeley Lights, Inc. | Automated detection and repositioning of micro-objects in microfluidic devices |
| EP3551745A4 (en) | 2016-12-12 | 2020-07-15 | Xcella Biosciences, Inc. | SCREENING METHODS AND SYSTEMS USING MICROCAPILLARY NETWORKS |
| CA3048645A1 (en) | 2016-12-30 | 2018-07-05 | The Regents Of The University Of California | Methods for selection and generation of genome edited t cells |
| US11156626B2 (en) | 2016-12-30 | 2021-10-26 | xCella Biosciences, Inc. | Multi-stage sample recovery system |
| WO2018207454A1 (ja) * | 2017-05-12 | 2018-11-15 | 株式会社ニコン | 対象物操作装置、及び対象物操作方法 |
| WO2018226900A2 (en) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | A htp genomic engineering platform for improving fungal strains |
| TWI832820B (zh) | 2017-07-21 | 2024-02-21 | 美商伯克利之光生命科技公司 | 抗原呈現合成表面、共價功能化表面、經活化之t細胞及其用途 |
| US11684917B2 (en) | 2017-09-29 | 2023-06-27 | Schlumberger Technology Corporation | Microfluidic technique for detection of multi-contact miscibility |
| EP3721209B1 (en) | 2017-10-15 | 2024-02-07 | Berkeley Lights, Inc. | Methods for in-pen assays |
| CN108300654B (zh) * | 2018-02-09 | 2021-08-17 | 华中科技大学同济医学院附属协和医院 | 评估可移动生物活体药代动力学的芯片及实时检测系统 |
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