CA2876178C - Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel - Google Patents

Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel Download PDF

Info

Publication number
CA2876178C
CA2876178C CA2876178A CA2876178A CA2876178C CA 2876178 C CA2876178 C CA 2876178C CA 2876178 A CA2876178 A CA 2876178A CA 2876178 A CA2876178 A CA 2876178A CA 2876178 C CA2876178 C CA 2876178C
Authority
CA
Canada
Prior art keywords
microorganism
biodiesel
clostridium
nucleic acid
substrate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2876178A
Other languages
English (en)
French (fr)
Other versions
CA2876178A1 (en
Inventor
Fungmin Liew
Michael Koepke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lanzatech NZ Inc
Original Assignee
Lanzatech New Zealand Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49769067&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2876178(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Lanzatech New Zealand Ltd filed Critical Lanzatech New Zealand Ltd
Publication of CA2876178A1 publication Critical patent/CA2876178A1/en
Application granted granted Critical
Publication of CA2876178C publication Critical patent/CA2876178C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C10L1/026—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01075—Long-chain-alcohol O-fatty-acyltransferase (2.3.1.75)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145—Clostridium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2876178A 2012-06-21 2013-06-21 Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel Active CA2876178C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261662467P 2012-06-21 2012-06-21
US61/662,467 2012-06-21
PCT/NZ2013/000108 WO2013191567A1 (en) 2012-06-21 2013-06-21 Recombinant microorganisms make biodiesel

Publications (2)

Publication Number Publication Date
CA2876178A1 CA2876178A1 (en) 2013-12-27
CA2876178C true CA2876178C (en) 2017-02-28

Family

ID=49769067

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2876178A Active CA2876178C (en) 2012-06-21 2013-06-21 Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel

Country Status (13)

Country Link
US (1) US9347076B2 (index.php)
EP (1) EP2864470B2 (index.php)
JP (1) JP6466836B2 (index.php)
KR (1) KR102097844B1 (index.php)
CN (1) CN104640977B (index.php)
BR (1) BR112014031876B1 (index.php)
CA (1) CA2876178C (index.php)
ES (1) ES2655214T5 (index.php)
IN (1) IN2014DN10507A (index.php)
MY (1) MY164882A (index.php)
NO (1) NO2953671T3 (index.php)
PL (1) PL2864470T5 (index.php)
WO (1) WO2013191567A1 (index.php)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201112231D0 (en) 2011-07-15 2011-08-31 Verdant Bioproducts Ltd Micro-organism
US20130323820A1 (en) 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel
SG11201605775UA (en) 2014-01-28 2016-08-30 Lanzatech New Zealand Ltd Method of producing a recombinant microorganism
JP6309304B2 (ja) * 2014-02-24 2018-04-11 積水化学工業株式会社 組換え細胞、並びに、１，４−ブタンジオールの生産方法
KR102531893B1 (ko) 2014-10-22 2023-05-11 란자테크 엔지, 인크. 멀티-스테이지 생물반응기 공정
US10113194B2 (en) 2014-10-22 2018-10-30 Lanzatech New Zealand Limited Gas testing unit and method
US10131884B2 (en) 2015-02-23 2018-11-20 Lanzatech New Zealand Limited Recombinant acetogenic bacterium for the conversion of methane to products
BR122023023155A2 (pt) * 2015-05-27 2024-03-05 Lanzatech Nz, Inc. Bactéria geneticamente modificada fixadora de c1 capaz de produzir pelo menos um produto derivado de corismato, e, método de fabricação de um produto de fermentação
MX2023003058A (es) 2015-10-13 2023-04-05 Lanzatech Nz Inc Bacteria diseñada por ingeniería genética que comprende una trayectoria de fermentación que genera energía.
EA201891335A1 (ru) 2015-12-03 2018-11-30 Ланцатек Нью Зилэнд Лимитед Добавление аргинина с целью повышения эффективности ацетогенов, ферментирующих газ
US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
US10358662B2 (en) 2016-02-01 2019-07-23 Lanzatech New Zealand Limited Integrated fermentation and electrolysis process
AU2017222688B2 (en) 2016-02-26 2023-05-25 Lanzatech Nz, Inc. CRISPR/Cas systems for C-1 fixing bacteria
CA3024114C (en) 2016-05-14 2020-02-04 Lanzatech, Inc. Microorganism with modified aldehyde:ferredoxin oxidoreductase activity and related methods
CN107217017B (zh) * 2017-05-27 2020-12-08 青岛农业大学 一株不动杆菌及其在石油降解中的应用
PH12020550073A1 (en) 2017-09-08 2022-07-18 Lanzatech Inc Processes and systems for metabolite production using hydrogen rich c1- containing substrates
MY196897A (en) 2017-12-19 2023-05-09 Lanzatech Inc Microorganisms and methods for the biological production of ethylene glycol
CN111683731B (zh) 2018-02-12 2022-09-02 朗泽科技有限公司 用于提高碳转化效率的工艺
CN111918957B (zh) 2018-04-20 2024-11-08 朗泽科技有限公司 间歇电解流
CN112955558A (zh) 2018-11-19 2021-06-11 朗泽科技有限公司 发酵和气化的集成
KR20240135053A (ko) 2019-02-08 2024-09-10 란자테크, 인크. 유사한 비등점 생성물의 회수 방법
MY197373A (en) 2019-07-11 2023-06-14 Lanzatech Inc Methods for optimizing gas utilization
CN111088193B (zh) * 2020-01-10 2022-09-09 南京工业大学 一种提高食甲基丁酸杆菌电转频率的方法
US11932818B2 (en) 2020-03-16 2024-03-19 Lanzatech, Inc. Tail gas of gas fermentation to dry gasification feedstock
JP2023518045A (ja) 2020-03-18 2023-04-27 ランザテク，インコーポレイテッド ガス基質からの２－フェニルエタノールの発酵産生
MX2022013570A (es) 2020-04-29 2022-11-30 Lanzatech Inc Produccion fermentativa de b-cetoadipato a partir de sustratos gaseosos.
EP4162056A4 (en) 2020-06-06 2024-05-29 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
JP7596523B2 (ja) 2021-02-08 2024-12-09 ランザテク，インコーポレイテッド 組換え微生物及びその使用
BR112023020761A2 (pt) 2021-04-09 2023-12-12 Lanzatech Inc Processo para fornecer uma matéria-prima, e, aparelho para fornecer uma matéria-prima de fermentação gasosa
WO2023004293A1 (en) 2021-07-20 2023-01-26 Lanzatech, Inc. Recombinant microorganisms and uses therefor
TW202307202A (zh) 2021-08-06 2023-02-16 美商朗澤科技有限公司 用於改良乙二醇之生物產生的微生物及方法
US12091648B2 (en) 2021-11-03 2024-09-17 Lanzatech, Inc. System and method for generating bubbles in a vessel
US12280331B2 (en) 2022-04-29 2025-04-22 Lanzatech, Inc. Low residence time gas separator
US12077800B2 (en) 2022-06-16 2024-09-03 Lanzatech, Inc. Liquid distributor system and process of liquid distribution
AU2023289395A1 (en) 2022-06-21 2025-01-09 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from c1-substrates
WO2023250382A1 (en) 2022-06-21 2023-12-28 Lanzatech, Inc. Microorganisms and methods for the continuous co-production of tandem repeat proteins and chemical products from c1-substrates
WO2024036187A1 (en) 2022-08-10 2024-02-15 Lanzatech, Inc. Carbon sequestration in soils with production of chemical products
US12281344B2 (en) 2023-06-05 2025-04-22 Lanzatech, Inc. Integrated gas fermentation
WO2024253883A1 (en) 2023-06-05 2024-12-12 Lanzatech, Inc. Integrated gas fermentation and carbon black processes

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173429A (en) 1990-11-09 1992-12-22 The Board Of Trustees Of The University Of Arkansas Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism
US5593886A (en) 1992-10-30 1997-01-14 Gaddy; James L. Clostridium stain which produces acetic acid from waste gases
UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
WO2002008438A2 (en) * 2000-07-25 2002-01-31 Bioengineering Resources, Inc. Methods for increasing the production of ethanol from microbial fermentation
DE102004052115A1 (de) * 2004-10-26 2006-04-27 Westfälische Wilhelms-Universität Münster Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung
ES2528482T3 (es) * 2006-05-19 2015-02-10 Ls9, Inc. Producción de ácidos grasos y derivados de los mismos
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
BRPI0809345A2 (pt) * 2007-03-28 2014-10-07 Ls9 Inc Células recombinantes, composições produzidas por estas, métodos para produzir derivados de ácidos graxos em uma célula recombinante, para aumentar a produção de derivados de ácidos graxos em uma célula recombinante, construto recombinante, vetores, métodos para aumentar a produção de derivados de ácidos graxos em uma célula hospedeira de produção, para produzir derivados de ácidos graxos, derivados de ácidos graxos, composições de biocombustível.
WO2009009391A2 (en) * 2007-07-06 2009-01-15 Ls9, Inc. Systems and methods for the production of fatty esters
CA2703622C (en) 2007-11-13 2014-12-16 Lanzatech New Zealand Limited Clostridium autoethanogenum strain and methods of use thereof to produce ethanol and acetate
GB0801787D0 (en) * 2008-01-31 2008-03-05 Reclaim Resources Ltd Apparatus and method for treating waste
US8048654B2 (en) * 2010-06-09 2011-11-01 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of fatty acids and esters
WO2010022090A1 (en) * 2008-08-18 2010-02-25 Ls9, Inc. Systems and methods for the production of mixed fatty esters
BRPI1014472A8 (pt) * 2009-04-10 2015-12-08 Ls9 Inc Método para produzir um derivado de ácido graxo, microrganismo engenheirado geneticamente, derivado de ácido graxo e composição de biocombustível
BRPI1009980A2 (pt) 2009-05-01 2015-08-25 Univ California Produtos de ésteres de ácidos graxos a partir de polímeros de biomassa
US8143037B2 (en) 2010-03-19 2012-03-27 Coskata, Inc. Ethanologenic Clostridium species, Clostridium coskatii
TW201224151A (en) * 2010-08-26 2012-06-16 Lanzatech New Zealand Ltd A process
US20110236941A1 (en) 2010-10-22 2011-09-29 Lanzatech New Zealand Limited Recombinant microorganism and methods of production thereof
AU2012221176B2 (en) 2011-02-25 2015-03-19 Lanzatech Nz, Inc. Recombinant microorganisms and uses therefor
CN102492672A (zh) * 2011-11-26 2012-06-13 宁波大学 一种催化二酰甘油酰基化的酶
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel

Also Published As

Publication number Publication date
CA2876178A1 (en) 2013-12-27
PL2864470T5 (pl) 2021-05-17
NO2953671T3 (index.php) 2018-03-17
ES2655214T5 (es) 2021-10-11
WO2013191567A1 (en) 2013-12-27
US20130344547A1 (en) 2013-12-26
CN104640977B (zh) 2019-04-12
EP2864470A4 (en) 2015-12-16
CN104640977A (zh) 2015-05-20
IN2014DN10507A (index.php) 2015-08-21
EP2864470B2 (en) 2020-12-23
EP2864470B1 (en) 2017-10-04
PL2864470T3 (pl) 2018-05-30
ES2655214T3 (es) 2018-02-19
BR112014031876A2 (pt) 2017-08-01
KR102097844B1 (ko) 2020-04-06
BR112014031876B1 (pt) 2022-05-10
JP2015519918A (ja) 2015-07-16
US9347076B2 (en) 2016-05-24
EP2864470A1 (en) 2015-04-29
MY164882A (en) 2018-01-30
KR20150023651A (ko) 2015-03-05
JP6466836B2 (ja) 2019-02-06

Similar Documents

Publication Publication Date Title
CA2876178C (en) Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel
CA2813431C (en) Production of butanol from carbon monoxide by a recombinant microorganism
CA3051235C (en) Genetically engineered bacterium comprising energy-generating fermentation pathway
CA2995872C (en) Recombinant clostridium bacterium and uses thereof in acetone production
CA2874832C (en) Recombinant microorganisms and uses therefor
CA2882276E (en) Recombinant microorganisms expressing a stereospecific diol dehydratase enzyme
EP4162056A1 (en) Microorganism with knock-in at acetolactate decarboxylase gene locus
JP2014161252A (ja) 組換え細胞

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20141209