CA2872149C - Viability staining method - Google Patents

Viability staining method Download PDF

Info

Publication number
CA2872149C
CA2872149C CA2872149A CA2872149A CA2872149C CA 2872149 C CA2872149 C CA 2872149C CA 2872149 A CA2872149 A CA 2872149A CA 2872149 A CA2872149 A CA 2872149A CA 2872149 C CA2872149 C CA 2872149C
Authority
CA
Canada
Prior art keywords
cells
fluorescent
membrane
viable
viable cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2872149A
Other languages
English (en)
French (fr)
Other versions
CA2872149A1 (en
Inventor
Eric STIMPSON
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Charles River Laboratories Inc
Original Assignee
Charles River Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Charles River Laboratories Inc filed Critical Charles River Laboratories Inc
Publication of CA2872149A1 publication Critical patent/CA2872149A1/en
Application granted granted Critical
Publication of CA2872149C publication Critical patent/CA2872149C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06Quantitative determination
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2872149A 2012-05-02 2013-05-02 Viability staining method Active CA2872149C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261641805P 2012-05-02 2012-05-02
US61/641,805 2012-05-02
US201361784759P 2013-03-14 2013-03-14
US61/784,759 2013-03-14
PCT/US2013/039347 WO2013166336A1 (en) 2012-05-02 2013-05-02 Viability staining method

Publications (2)

Publication Number Publication Date
CA2872149A1 CA2872149A1 (en) 2013-11-07
CA2872149C true CA2872149C (en) 2019-03-19

Family

ID=48692634

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2872149A Active CA2872149C (en) 2012-05-02 2013-05-02 Viability staining method

Country Status (7)

Country Link
US (4) US8993259B2 (https=)
EP (1) EP2769218B1 (https=)
JP (1) JP6285915B2 (https=)
CN (1) CN104662425B (https=)
AU (1) AU2013256174B2 (https=)
CA (1) CA2872149C (https=)
WO (1) WO2013166336A1 (https=)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPQ899700A0 (en) 2000-07-25 2000-08-17 Borody, Thomas Julius Probiotic recolonisation therapy
PH12013500224A1 (en) 2010-08-04 2013-04-08 Borody Thomas J Compositions for fecal floral transplantation and methods for making and using them and devices for delivering them
MX2013010343A (es) 2011-03-09 2014-04-30 Univ Minnesota Composicion y metodos para el transplante de microbiota de colon.
EP2780707B1 (en) 2012-05-02 2016-02-24 Charles River Laboratories, Inc. Method of detecting viable cells in a cell sample
EP2769204B1 (en) 2012-05-02 2016-02-17 Charles River Laboratories, Inc. Cell capture system and use thereof
AU2013256174B2 (en) 2012-05-02 2017-03-02 Charles River Laboratories, Inc. Viability staining method
WO2013176774A1 (en) 2012-05-25 2013-11-28 Arizona Board Of Regents Microbiome markers and therapies for autism spectrum disorders
CN105874052B (zh) * 2013-11-04 2019-05-21 查尔斯河实验室公司 过滤系统和其使用
WO2015066727A1 (en) * 2013-11-04 2015-05-07 Charles River Laboratories, Inc. Method for detecting viable cells in a cell sample
US20150218612A1 (en) * 2014-02-06 2015-08-06 Tacount Exact Ltd. Apparatus, system and method for live bacteria microscopy
WO2016053931A1 (en) * 2014-09-30 2016-04-07 Corning Incorporated Membrane with improved fluorescent spectral blocking efficiency
EP3250907A4 (en) 2015-01-26 2018-08-29 Bacterioscan Ltd. Laser-scatter measurement instrument having carousel-based fluid sample arrangement
US10060850B2 (en) 2015-04-03 2018-08-28 Captl Llc Particle detection using reflective surface
CA2985827A1 (en) * 2015-05-14 2016-11-17 Crestovo Holdings Llc Compositions for fecal floral transplantation and methods for making and using them and devices for delivering them
AU2016268158B2 (en) 2015-05-22 2022-03-17 Arizona Board Of Regents On Behalf Of Arizona State University Methods for treating autism spectrum disorder and associated symptoms
WO2016196567A1 (en) * 2015-06-01 2016-12-08 Nexcelom Bioscience Llc Methods for cell count and viability measurements
US10613096B2 (en) 2015-08-28 2020-04-07 Captl Llc Multi-spectral microparticle-fluorescence photon cytometry
JP6660712B2 (ja) 2015-11-10 2020-03-11 株式会社Screenホールディングス 分類器構成方法および細胞の生死判定方法
US20170360848A1 (en) 2016-06-15 2017-12-21 Arizona Board Of Regents On Behalf Of Arizona State University Methods for treating autism spectrum disorder and associated symptoms
US10849936B2 (en) 2016-07-01 2020-12-01 Regents Of The University Of Minnesota Compositions and methods for C. difficile treatment
US20180036352A1 (en) 2016-08-03 2018-02-08 Crestovo Holdings Llc Methods for treating ulcerative colitis
WO2018071537A1 (en) 2016-10-11 2018-04-19 Crestovo Holdings Llc Compositions and methods for treating multiple sclerosis and related disorders
US10092601B2 (en) 2016-10-11 2018-10-09 Crestovo Holdings Llc Compositions and methods for treating multiple sclerosis and related disorders
US11213549B2 (en) 2016-10-11 2022-01-04 Finch Therapeutics Holdings Llc Compositions and method for treating primary sclerosing cholangitis and related disorders
WO2018187464A1 (en) 2017-04-05 2018-10-11 Crestovo Holdings Llc Compositions and methods for treating diverticulitis and related disorders
CN110831606A (zh) 2017-04-05 2020-02-21 克雷斯顿沃控股公司 治疗帕金森氏病(pd)和相关疾病的组合物和方法
WO2018191497A2 (en) 2017-04-13 2018-10-18 Captl Llc Photon counting and spectroscopy
USD829338S1 (en) * 2017-05-15 2018-09-25 Charles River Laboratories, Inc. Membrane holder
JP2020521760A (ja) 2017-05-26 2020-07-27 クレストヴォ・ホールディングス・エルエルシー 糞便微生物ベースの治療剤を含む凍結乾燥組成物ならびにそれを製造および使用する方法
WO2019032573A1 (en) 2017-08-07 2019-02-14 Finch Therapeutics, Inc. COMPOSITIONS AND METHODS FOR MAINTAINING AND RESTORING A HEALTHY INTESTINAL BARRIER
GB201716883D0 (en) 2017-10-13 2017-11-29 Q-Linea Ab Method for determining the concentration of intact microorganisms in a sample
US20210181085A1 (en) * 2017-10-26 2021-06-17 Essenlix Corporation Rapid measurement of platelets
EP3805401B1 (en) 2018-06-01 2023-09-27 Frontier Pharma, Inc. Image processing method, medicine sensitivity test method and image processing device
JP6627069B2 (ja) 2018-06-01 2020-01-08 株式会社フロンティアファーマ 画像処理方法、薬剤感受性試験方法および画像処理装置
US11166990B2 (en) 2018-07-13 2021-11-09 Finch Therapeutics Holdings Llc Methods and compositions for treating ulcerative colitis
CN112673315B (zh) * 2018-07-20 2025-02-25 伊鲁米那股份有限公司 树脂组合物和并入树脂组合物的流动池
US11911419B2 (en) 2018-09-27 2024-02-27 Finch Therapeutics Holdings Llc Compositions and methods for treating epilepsy and related disorders
US10564100B1 (en) 2018-10-12 2020-02-18 SageMedic Corporation Analysis of viable and nonviable cells
US12290538B2 (en) 2019-07-19 2025-05-06 Finch Therapeutics Holdings Llc Methods and products for treatment of gastrointestinal disorders
US20230131415A1 (en) * 2020-04-15 2023-04-27 Rapid Micro Biosystems, Inc. Attenuated-background microbiological nutrient media and methods of using the same
WO2025101448A1 (en) * 2023-11-06 2025-05-15 Rapid Micro Biosystems, Inc. Rinse solution for microbial detection systems

Family Cites Families (112)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1256360A (en) 1984-02-10 1989-06-27 John W. Bornhoeft Apparatus and method for determining the effectiveness of sterlization
EP0215066A1 (en) 1985-02-27 1987-03-25 University Of Cincinnati Viable microorganism detection by induced fluorescence
US4739416A (en) 1986-09-15 1988-04-19 Matrix Instruments Inc. Digital image reproduction
US4725891A (en) 1986-09-15 1988-02-16 Matrix Instruments Inc. Film image digitizer
US4783401A (en) 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
JPH0813930B2 (ja) 1987-12-28 1996-02-14 三井東圧化学株式会社 近赤外線吸収フィルター用高純度アントラキノン系色素
US4930893A (en) 1988-03-25 1990-06-05 Molecular Dynamics Electrophoresis imaging system
US4838632A (en) 1988-05-06 1989-06-13 Lumisys Inc. Two-dimensional beam scanner
US5073488A (en) 1988-11-29 1991-12-17 Minnesota Mining And Manufacturing Company Rapid method for determining efficacy of a sterilization cycle and rapid read-out biological indicator
WO1992002632A1 (en) 1990-07-30 1992-02-20 Sierra Cytometry Fluorescent dyes for identification and enumeration of viable cells in milk
US5185450A (en) 1991-02-19 1993-02-09 University Of South Florida Tetrazolium compounds for cell viability assays
US5296341A (en) 1991-03-05 1994-03-22 Lumisys, Inc. Green light laser imaging method
US5172419A (en) 1991-03-05 1992-12-15 Lumisys, Inc. Medical image processing system
US5314805A (en) 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
US5137609A (en) 1992-01-31 1992-08-11 Biometric Imaging Inc. Differential separation assay
US5843680A (en) 1992-01-31 1998-12-01 Biometric Imaging, Inc. Differential separation assay methods and test kits
US5221454A (en) 1992-01-31 1993-06-22 Biometric Imaging Inc. Differential separation assay
DE69311944T2 (de) 1992-04-01 1997-11-20 Nihon Millipore Kogyo K K Verfahren zur Bestimmung der Anzahl lebender Mikroorganismen
JP3228812B2 (ja) 1993-02-10 2001-11-12 日本マイクロリス株式会社 生菌数を測定する方法
US5547849A (en) 1993-02-17 1996-08-20 Biometric Imaging, Inc. Apparatus and method for volumetric capillary cytometry
US5585246A (en) 1993-02-17 1996-12-17 Biometric Imaging, Inc. Method for preparing a sample in a scan capillary for immunofluorescent interrogation
US5445946A (en) 1993-04-13 1995-08-29 Molecular Probes, Inc. Intravacuolar stains for yeast and other fungi
US5534416A (en) 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
US5545535A (en) 1993-04-13 1996-08-13 Molecular Probes, Inc. Fluorescent assay for bacterial gram reaction
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5437980A (en) 1993-05-17 1995-08-01 Molecular Probes, Inc. Phenanthridium dye staining of nucleic acids in living cells
FR2707665A1 (fr) 1993-06-28 1995-01-20 Chemunex Procédé de mesure et d'évaluation de la vitalité des microorganismes et ses applications.
IES940182A2 (en) 1994-03-01 1995-11-29 Teagasc Agric Food Dev Authori "Rapid detection of bacteria in liquid cultures"
US5550032A (en) 1994-05-27 1996-08-27 George Mason University Biological assay for microbial contamination
EP0713087B1 (en) 1994-11-17 1999-04-14 Chemunex Apparatus and process for rapid and ultrasensitive detection and counting of microorganisms by fluorescence
US5603900A (en) 1995-05-19 1997-02-18 Millipore Investment Holdings Limited Vacuum filter device
US5565678A (en) 1995-06-06 1996-10-15 Lumisys, Inc. Radiographic image quality assessment utilizing a stepped calibration target
WO1997012226A1 (en) 1995-09-25 1997-04-03 Tencor Instruments Improved system for surface inspection
HUP9902028A2 (hu) 1995-11-08 1999-11-29 Pioneer Hi-Bred International, Inc. Eljárások mikrobatenyészetek életképességének becslésére
EP0881489A1 (en) 1995-12-29 1998-12-02 Wako Pure Chemical Industries Ltd Method for determining viable cell count
US6459805B1 (en) * 1996-03-26 2002-10-01 Childrens Hospital Los Angeles Fluorescence digital imaging microscopy system
DE19621312A1 (de) 1996-05-28 1997-12-04 Bayer Ag Maskierung der Hintergrundfluoreszenz und Signalverstärkung bei der optischen Analyse biologisch medizinischer Assays
US5956146A (en) 1997-01-29 1999-09-21 Victor Company Of Japan, Ltd. Birefringence measuring apparatus for optical disc substrate
US6221621B1 (en) 1997-03-06 2001-04-24 Bard Diagnostic Sciences, Inc. Methods of screening for colorectal cancers in which a complement Factor I or related protein is associated
FR2764305B1 (fr) 1997-06-04 2000-10-06 Chemunex Procede de detection et de numeration de cellules viables dans un echantillon biologique et kit pour sa mise en oeuvre
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6043506A (en) 1997-08-13 2000-03-28 Bio-Rad Laboratories, Inc. Multi parameter scanner
US5922617A (en) 1997-11-12 1999-07-13 Functional Genetics, Inc. Rapid screening assay methods and devices
CN1230682C (zh) 1997-12-30 2005-12-07 埃佩多夫阵列技术股份有限公司 探测和量化目标分子的方法及探测和读取装置
US6080868A (en) 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
EP1101106A4 (en) 1998-07-28 2006-05-03 Bd Biosciences Systems And Rea ARRANGEMENT AND METHOD FOR THE TEST FOR CELL MOBILITY
US6130745A (en) 1999-01-07 2000-10-10 Biometric Imaging, Inc. Optical autofocus for use with microtiter plates
DE60002562T2 (de) 1999-02-26 2004-04-15 Cellomics, Inc. Ein system für zellbasierte reihenuntersuchungen
JP2000304688A (ja) 1999-04-16 2000-11-02 Canon Inc 基板測定方法および装置
US6181413B1 (en) 1999-09-03 2001-01-30 Biometric Imaging, Inc. Displacing volume in field of view
AU4134801A (en) 1999-10-25 2001-05-30 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
JP2001228088A (ja) 2000-02-18 2001-08-24 Nippon Laser & Electronics Lab 生体試料光学的走査装置
JP2001242082A (ja) 2000-02-29 2001-09-07 Nippon Laser & Electronics Lab 生体試料光学的走査装置
GB0008787D0 (en) 2000-04-10 2000-05-31 Norsk Naeringsmiddelforskning A method of cell detection
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US7118878B1 (en) 2000-06-09 2006-10-10 Promega Corporation Method for increasing luminescence assay sensitivity
IL139593A (en) 2000-11-09 2010-12-30 Biogem Optical Ltd Method for the detection of viable microorganisms
US6633368B2 (en) 2001-01-02 2003-10-14 Becton, Dickinson And Company Method for determining the volume of single red blood cells
US6714287B2 (en) 2001-01-02 2004-03-30 Becton, Dickinson And Company Apparatus for determining the volume of single red blood cells
EP1364207A4 (en) 2001-01-25 2005-02-09 Thaco Res Ltd FAST PROCEDURES FOR MICROBIAL TYPING AND COUNTING
US7016087B2 (en) 2001-08-08 2006-03-21 Becton Dickinson And Company Photon efficient scanner
US6750457B2 (en) 2001-08-29 2004-06-15 Becton Dickinson And Company System for high throughput analysis
EP2311934B1 (en) 2001-09-06 2013-06-05 Rapid Micro Biosystems, Inc. Rapid detection of replicating cells
US7205100B2 (en) 2002-10-10 2007-04-17 Advanced Analytical Technologies, Inc. Methods for reducing background fluorescence
ES2239886B1 (es) 2003-11-05 2006-12-16 Universidad De Barcelona Metodo y aparato para la determinacion de la viabilidad celular.
EP1699351B1 (en) 2003-12-22 2011-05-04 Lightouch Medical, Inc. Process for determination of cell viability
WO2005088287A1 (en) 2004-03-17 2005-09-22 National Research Council Of Canada Method and apparatus for the detection of microorganisms
US20060129327A1 (en) 2004-07-29 2006-06-15 Kim Myung L Ultrasensitive sensor and rapid detection of analytes
EP1624071B1 (en) * 2004-08-06 2008-10-22 Fuji Electric Holdings Co., Ltd. Method of detecting viable cells
US7018804B1 (en) 2004-12-03 2006-03-28 Orion Biosolutions, Inc. Determination of cell viability and phenotype
US20060121443A1 (en) 2004-12-03 2006-06-08 Orion Biosolutions, Inc. Determination of cell viability
EP1853387A4 (en) 2005-02-22 2009-11-11 Univ Cincinnati DETERMINATION OF LIVABLE MICROORGANISMS BY COATED PARAMAGNETIC BEADS
CA2623597A1 (en) 2005-09-16 2007-03-16 The Charles Stark Draper Laboratory, Inc. Reaction sensing in living cells
US8364409B2 (en) 2006-01-12 2013-01-29 Biosense Technologies, Inc. Method and composition for rapid viability testing of cells
DE102006022877B4 (de) 2006-05-15 2009-01-29 Sartorius Stedim Biotech Gmbh Verfahren zur Bestimmung der Viabilität von Zellen in Zellkulturen
GB0615302D0 (en) 2006-08-01 2006-09-13 Biotrace Internat Plc Assay Method For The Detection Of Viable Microbial Cells In A Sample
US20080153125A1 (en) 2006-11-10 2008-06-26 Advanced Analytical Technologies, Inc. Rapid detection of microorganisms in fluids
CN101201312A (zh) * 2006-12-15 2008-06-18 北京林业大学 一种中性红染色法检测藻细胞活性的方法
EP2142665B1 (en) 2007-04-13 2013-11-27 Promega Corporation Luminescent live and dead cell assay
US20080305514A1 (en) 2007-06-06 2008-12-11 Alcon Research, Ltd. Method for detecting microbes
WO2009029039A1 (en) 2007-08-28 2009-03-05 Ridgeview Instruments Ab Method for measurement of cell viability
MX2010005079A (es) 2007-11-09 2010-05-20 Genzyme Corp Metodos para medir la viabilidad celular sin usar celulas de control.
WO2009082218A1 (en) 2007-12-20 2009-07-02 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Real-time method for the detection of viable micro-organisms
US20110110861A1 (en) 2008-05-13 2011-05-12 Mireille Hanna Lahoud Methods of Detecting Cells with a Disrupted Cell Membrane, Cells Infected with A Pathogen, Dying Cells or Dead Cells
EP2312943B1 (en) 2008-07-14 2019-11-06 Chemometec A/S Method for assessing viable cells and the use of n-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (dacm) or n-(9-acridinyl)maleimide (nam) for assessment of apoptosis.
CN101344476B (zh) * 2008-08-20 2011-03-16 吉林农业大学 细菌活的非可培养状态荧光显微镜观察与计数方法
ES2540544T3 (es) 2009-05-04 2015-07-10 Novartis Ag Microensayo rápido de esterilidad
US8148515B1 (en) 2009-06-02 2012-04-03 Biotium, Inc. Detection using a dye and a dye modifier
WO2010151131A2 (en) 2009-06-26 2010-12-29 Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek Tno Apparatus for detecting viable microorganisms or spores in a sample and use thereof.
SG177738A1 (en) 2009-07-24 2012-02-28 Morinaga Milk Industry Co Ltd Method and kit for detecting microorganisms
CA2781516C (en) 2010-03-26 2016-01-26 Morinaga Milk Industry Co., Ltd. Culture medium for specific growth, detection, and enumeration of bifidobacterium breve
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
US20110294206A1 (en) 2010-05-28 2011-12-01 California Institute Of Technology Methods and design of membrane filters
JP5717846B2 (ja) 2010-06-03 2015-05-13 ビーエーエスエフ ソシエタス・ヨーロピアBasf Se 微生物の検出及び定量化
WO2012051437A1 (en) 2010-10-13 2012-04-19 Life Technologies Corporation Compositions and assays for determining cell viability
CA2815951A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Measurement system for fluorescent detection, and method therefor
CA2816014A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Method and device for fluorescent measurement of samples
WO2012059785A1 (en) 2010-11-03 2012-05-10 Reametrix Inc. Methods for calibrating a raman spectrometer, and spectrometer
EP2637787A1 (en) 2010-11-08 2013-09-18 Reactrix Systems, Inc. Sample assembly for a measurement device
US9446411B2 (en) 2011-02-24 2016-09-20 Reametrix, Inc. Sample assembly for a measurement device
EP2780707B1 (en) 2012-05-02 2016-02-24 Charles River Laboratories, Inc. Method of detecting viable cells in a cell sample
EP2769204B1 (en) 2012-05-02 2016-02-17 Charles River Laboratories, Inc. Cell capture system and use thereof
AU2013256174B2 (en) 2012-05-02 2017-03-02 Charles River Laboratories, Inc. Viability staining method
USD717352S1 (en) 2013-03-14 2014-11-11 Reametrix, Inc. Chuck
USD705438S1 (en) 2013-03-14 2014-05-20 Charles River Laboratories, Inc. Base
USD698939S1 (en) 2013-03-14 2014-02-04 Charles River Laboratories, Inc. Lid
USD705439S1 (en) 2013-03-14 2014-05-20 Charles River Laboratories Base with membrane and support
USD704854S1 (en) 2013-03-14 2014-05-13 Charles River Laboratories, Inc. Stage
USD717460S1 (en) 2013-03-14 2014-11-11 Reametrix, Inc. Cell detection system

Also Published As

Publication number Publication date
CA2872149A1 (en) 2013-11-07
AU2013256174A1 (en) 2014-11-20
US20150368689A1 (en) 2015-12-24
US20130316394A1 (en) 2013-11-28
US20130309686A1 (en) 2013-11-21
EP2769218B1 (en) 2016-08-10
JP2015521037A (ja) 2015-07-27
US8993259B2 (en) 2015-03-31
AU2013256174B2 (en) 2017-03-02
EP2769218A1 (en) 2014-08-27
CN104662425B (zh) 2017-10-10
CN104662425A (zh) 2015-05-27
US20150368690A1 (en) 2015-12-24
US8993260B2 (en) 2015-03-31
JP6285915B2 (ja) 2018-02-28
WO2013166336A1 (en) 2013-11-07

Similar Documents

Publication Publication Date Title
CA2872149C (en) Viability staining method
EP2780707B1 (en) Method of detecting viable cells in a cell sample
US10976258B2 (en) Porous planar cell capture system and method of use
AU2007207450B2 (en) Method for rapid detection and evaluation of cultured cell growth
JP2017514473A (ja) サンプルの膜またはアガロース媒体上で増殖する微小コロニーを検出する方法および無菌テスト装置
Huang et al. Light-addressable measurements of cellular oxygen consumption rates in microwell arrays based on phase-based phosphorescence lifetime detection
JP4830103B2 (ja) 生理活性判定方法、及び生理活性判定用キット
US20160298162A1 (en) Method for detecting viable cells in a cell sample

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20180430

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 12TH ANNIV.) - STANDARD

Year of fee payment: 12

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250408

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250408

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 13TH ANNIV.) - STANDARD

Year of fee payment: 13

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20260407

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20260407