CA2861270C - Perfusion bioreactor systems comprising a cell aggregate trap and methods of operating the same - Google Patents

Perfusion bioreactor systems comprising a cell aggregate trap and methods of operating the same Download PDF

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Publication number
CA2861270C
CA2861270C CA2861270A CA2861270A CA2861270C CA 2861270 C CA2861270 C CA 2861270C CA 2861270 A CA2861270 A CA 2861270A CA 2861270 A CA2861270 A CA 2861270A CA 2861270 C CA2861270 C CA 2861270C
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CA
Canada
Prior art keywords
cells
cell
trap
tissue culture
culture fluid
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Active
Application number
CA2861270A
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English (en)
French (fr)
Other versions
CA2861270A1 (en
Inventor
Medhi Saghafi
Ricaredo Matanguihan
Chetan Goudar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Healthcare LLC
Original Assignee
Bayer Healthcare LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Publication of CA2861270A1 publication Critical patent/CA2861270A1/en
Application granted granted Critical
Publication of CA2861270C publication Critical patent/CA2861270C/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22—Settling tanks; Sedimentation by gravity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2861270A 2012-01-18 2013-01-15 Perfusion bioreactor systems comprising a cell aggregate trap and methods of operating the same Active CA2861270C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261587940P 2012-01-18 2012-01-18
US61/587,940 2012-01-18
PCT/US2013/021533 WO2013109520A1 (en) 2012-01-18 2013-01-15 Perfusion bioreactor systems and methods of operating the same

Publications (2)

Publication Number Publication Date
CA2861270A1 CA2861270A1 (en) 2013-07-25
CA2861270C true CA2861270C (en) 2021-08-03

Family

ID=47605793

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2861270A Active CA2861270C (en) 2012-01-18 2013-01-15 Perfusion bioreactor systems comprising a cell aggregate trap and methods of operating the same

Country Status (6)

Country Link
EP (1) EP2804943A1 (zh)
JP (1) JP6227562B2 (zh)
CN (1) CN104160013B (zh)
CA (1) CA2861270C (zh)
HK (1) HK1199056A1 (zh)
WO (1) WO2013109520A1 (zh)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3047013B1 (en) * 2013-09-16 2021-08-18 Genzyme Corporation Methods and systems for processing a cell culture
EP3089800A4 (en) * 2013-12-30 2018-09-12 GE Healthcare Bio-Sciences Corp. Apparatus for cell cultivation
US20170292104A1 (en) * 2014-09-17 2017-10-12 Massachusetts Institute Of Technology Microfluidic system and method for perfusion bioreactor cell retention
US9944894B2 (en) * 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
JP6943449B2 (ja) * 2016-03-21 2021-09-29 グローバル・ライフ・サイエンシズ・ソリューションズ・ユーエスエー・エルエルシー 撹拌タンクバイオリアクタを用いた多能性幹細胞の増殖および継代
CN107541464B (zh) * 2017-10-31 2021-04-30 山东亦度生物技术有限公司 增强型的细胞微载体灌流培养的截留方法
WO2019226618A1 (en) * 2018-05-22 2019-11-28 Nantkwest, Inc. Methods and systems for cell bed formation during bioprocessing
JP7087704B2 (ja) * 2018-06-13 2022-06-21 株式会社Ihi 細胞培養システム及び細胞培養方法
US11028359B2 (en) * 2018-09-11 2021-06-08 Global Life Sciences Solutions Usa Llc Separation devices, associated methods, and systems
GB201904083D0 (en) * 2019-03-25 2019-05-08 Ge Healthcare Bio Sciences Ab A bioreactor system
EP3835403A1 (en) 2019-12-11 2021-06-16 Sartorius Stedim Biotech GmbH Automated medium exchange strategy for suspension cells
JP7406205B2 (ja) * 2020-03-24 2023-12-27 横河電機株式会社 メタン生成装置
WO2024092004A1 (en) * 2022-10-26 2024-05-02 National Resilience, Inc. Bioreactor systems and methods of operating same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05153973A (ja) * 1991-12-03 1993-06-22 Hitachi Ltd 細胞培養方法及び装置
US6338964B1 (en) 1999-05-07 2002-01-15 Bayer Corporation Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration
CN1112442C (zh) * 2000-06-14 2003-06-25 华东理工大学 细胞灌注培养过程中的细胞截留分离方法及其装置
WO2003020919A2 (en) * 2001-08-31 2003-03-13 Bayer Healthcare Ag A unit and a process for carrying out high cell density fermentation
UA99249C2 (ru) 2004-09-30 2012-08-10 БАЙЕР ХЕЛСКЕА ЭлЭлСи Устройство и способ для интегрированного непрерывного производства биологических молекул
US20090280565A1 (en) * 2005-12-22 2009-11-12 Corporation De L'ecole Polytechique Montr'eal High-rate perfusion bioreactor
US8512566B2 (en) * 2009-12-11 2013-08-20 General Electric Company Disposable fluid path systems and methods for processing complex biological materials

Also Published As

Publication number Publication date
HK1199056A1 (zh) 2015-06-19
JP2015503929A (ja) 2015-02-05
WO2013109520A1 (en) 2013-07-25
EP2804943A1 (en) 2014-11-26
CN104160013A (zh) 2014-11-19
CA2861270A1 (en) 2013-07-25
JP6227562B2 (ja) 2017-11-08
CN104160013B (zh) 2017-07-28

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Effective date: 20180111