CA2851997C - Chimeric non-human animal carrying human hepatocyte - Google Patents

Chimeric non-human animal carrying human hepatocyte Download PDF

Info

Publication number
CA2851997C
CA2851997C CA2851997A CA2851997A CA2851997C CA 2851997 C CA2851997 C CA 2851997C CA 2851997 A CA2851997 A CA 2851997A CA 2851997 A CA2851997 A CA 2851997A CA 2851997 C CA2851997 C CA 2851997C
Authority
CA
Canada
Prior art keywords
human
mouse
mice
cyp3a
scid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2851997A
Other languages
English (en)
French (fr)
Other versions
CA2851997A1 (en
Inventor
Mitsuo Oshimura
Yasuhiro Kazuki
Chise Mukaidani
Takashi Shimada
Masakazu KAKUNI
Satoko HAMAMURA
Hidetaka KAMIMURA
Akio Kawamura
Naoyuki Nakada
Masato OHBUCHI
Kota Kato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tottori University
Phoenixbio Co Ltd
Original Assignee
Tottori University
Phoenixbio Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tottori University, Phoenixbio Co Ltd filed Critical Tottori University
Publication of CA2851997A1 publication Critical patent/CA2851997A1/en
Application granted granted Critical
Publication of CA2851997C publication Critical patent/CA2851997C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/12—Animals modified by administration of exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Organic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2851997A 2011-10-13 2012-10-12 Chimeric non-human animal carrying human hepatocyte Expired - Fee Related CA2851997C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2011226233A JP5883265B2 (ja) 2011-10-13 2011-10-13 ヒト肝細胞を担持するキメラ非ヒト動物
JP2011-226233 2011-10-13
PCT/JP2012/077019 WO2013054949A1 (ja) 2011-10-13 2012-10-12 ヒト肝細胞を担持するキメラ非ヒト動物

Publications (2)

Publication Number Publication Date
CA2851997A1 CA2851997A1 (en) 2013-04-18
CA2851997C true CA2851997C (en) 2018-07-10

Family

ID=48081988

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2851997A Expired - Fee Related CA2851997C (en) 2011-10-13 2012-10-12 Chimeric non-human animal carrying human hepatocyte

Country Status (5)

Country Link
US (1) US9420769B2 (cg-RX-API-DMAC7.html)
EP (1) EP2767160B1 (cg-RX-API-DMAC7.html)
JP (1) JP5883265B2 (cg-RX-API-DMAC7.html)
CA (1) CA2851997C (cg-RX-API-DMAC7.html)
WO (1) WO2013054949A1 (cg-RX-API-DMAC7.html)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6496107B2 (ja) * 2014-05-08 2019-04-03 株式会社フェニックスバイオ 高尿酸血症モデル
WO2018005471A1 (en) 2016-06-27 2018-01-04 Baylor College Of Medicine Human liver chimeric non-human animal with deficient p450 oxidoreductase and methods of using same
KR20220035926A (ko) * 2019-07-26 2022-03-22 앰비스 메디신즈 인코포레이티드 간세포 생성을 위한 방법 및 조성물
JP7037789B2 (ja) * 2019-12-23 2022-03-17 積水メディカル株式会社 ヒト肝細胞キメラ動物におけるヒト肝細胞置換率の測定方法
EP4408169A4 (en) * 2021-09-30 2025-10-29 Univ Southern California Human hepatocyte culture medium and conditioned medium of in vitro cultured human hepatocyte cells and their uses
CN118265448A (zh) * 2021-09-30 2024-06-28 南加州大学 人肝细胞培养基和体外培养的人肝细胞的条件化培养基及其用途

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3266766B2 (ja) 1994-08-23 2002-03-18 科学技術振興事業団 ローン性増殖能を有する肝実質細胞とその取得方法、並びにその継代培養方法
JP3211941B2 (ja) 1996-12-26 2001-09-25 科学技術振興事業団 ヒト小型肝細胞の取得方法と、この細胞の初代培養および継代培養方法
WO2001011951A1 (fr) * 1999-08-13 2001-02-22 Kirin Beer Kabushiki Kaisha Souris renfermant un cytochrome p450 humain
US6995299B2 (en) * 1999-11-02 2006-02-07 University Of Connecticut Propagation of human hepatocytes in non-human animals
US6509514B1 (en) 2000-03-17 2003-01-21 Kmt Hepatech, Inc. Chimeric animal model susceptible to human hepatitis C virus infection
JP2002045087A (ja) 2000-05-19 2002-02-12 Japan Science & Technology Corp キメラ動物
EP1496066A4 (en) * 2002-03-25 2006-09-13 Japan Science & Tech Agency ANTIBODY RECOGNIZING PROLIFERATIVE HUMAN LIVER CELLS, PROLIFERATIVE HUMAN LIVER CELLS, AND FUNCTIONAL HUMAN LIVER CELLS
WO2008001614A1 (fr) 2006-06-29 2008-01-03 Hiroshima Industrial Promotion Organization Modèle animalier de stéatohépatite non alcoolique et modèle animalier de stéatose hépatique
GB0720552D0 (en) * 2007-10-19 2007-11-28 Iti Scotland Ltd Multiple cluster knockout
JP4997544B2 (ja) * 2007-11-14 2012-08-08 国立大学法人鳥取大学 ヒトチトクロームｐ４５０遺伝子（クラスター）を含む哺乳動物人工染色体ベクター及びそれを保持する非ヒト哺乳動物
JP5557217B2 (ja) * 2010-01-06 2014-07-23 国立大学法人鳥取大学 マウス人工染色体ベクター
KR20110116817A (ko) 2010-04-20 2011-10-26 삼성전기주식회사 열전 모듈을 이용한 건축용 단열재

Also Published As

Publication number Publication date
CA2851997A1 (en) 2013-04-18
EP2767160A1 (en) 2014-08-20
WO2013054949A1 (ja) 2013-04-18
US9420769B2 (en) 2016-08-23
JP2013085485A (ja) 2013-05-13
US20140241991A1 (en) 2014-08-28
JP5883265B2 (ja) 2016-03-09
EP2767160A4 (en) 2015-07-01
EP2767160B1 (en) 2017-01-18

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20140423

MKLA Lapsed

Effective date: 20201013