JP5883265B2 - ヒト肝細胞を担持するキメラ非ヒト動物 - Google Patents

ヒト肝細胞を担持するキメラ非ヒト動物 Download PDF

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Publication number
JP5883265B2
JP5883265B2 JP2011226233A JP2011226233A JP5883265B2 JP 5883265 B2 JP5883265 B2 JP 5883265B2 JP 2011226233 A JP2011226233 A JP 2011226233A JP 2011226233 A JP2011226233 A JP 2011226233A JP 5883265 B2 JP5883265 B2 JP 5883265B2
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human
human animal
cyp3a
mice
scid
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Expired - Fee Related
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JP2011226233A
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English (en)
Japanese (ja)
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JP2013085485A5 (cg-RX-API-DMAC7.html
JP2013085485A (ja
Inventor
光雄 押村
光雄 押村
康宏 香月
康宏 香月
知世 向谷
知世 向谷
島田　卓
卓 島田
雅和 加国
雅和 加国
理子 浜村
理子 浜村
秀隆 神村
秀隆 神村
章生 河村
章生 河村
直之 中田
直之 中田
雅人 大渕
雅人 大渕
弘太 加藤
弘太 加藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tottori University
Phoenixbio Co Ltd
Original Assignee
Tottori University
Phoenixbio Co Ltd
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Filing date
Publication date
Application filed by Tottori University, Phoenixbio Co Ltd filed Critical Tottori University
Priority to JP2011226233A priority Critical patent/JP5883265B2/ja
Priority to EP12840420.9A priority patent/EP2767160B1/en
Priority to CA2851997A priority patent/CA2851997C/en
Priority to US14/351,481 priority patent/US9420769B2/en
Priority to PCT/JP2012/077019 priority patent/WO2013054949A1/ja
Publication of JP2013085485A publication Critical patent/JP2013085485A/ja
Publication of JP2013085485A5 publication Critical patent/JP2013085485A5/ja
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • G01N33/5088—Supracellular entities, e.g. tissue, organisms of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/12—Animals modified by administration of exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Organic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2011226233A 2011-10-13 2011-10-13 ヒト肝細胞を担持するキメラ非ヒト動物 Expired - Fee Related JP5883265B2 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2011226233A JP5883265B2 (ja) 2011-10-13 2011-10-13 ヒト肝細胞を担持するキメラ非ヒト動物
EP12840420.9A EP2767160B1 (en) 2011-10-13 2012-10-12 Chimeric non-human animal carrying human hepatocyte
CA2851997A CA2851997C (en) 2011-10-13 2012-10-12 Chimeric non-human animal carrying human hepatocyte
US14/351,481 US9420769B2 (en) 2011-10-13 2012-10-12 Chimeric non-human animal carrying human hepatocyte
PCT/JP2012/077019 WO2013054949A1 (ja) 2011-10-13 2012-10-12 ヒト肝細胞を担持するキメラ非ヒト動物

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2011226233A JP5883265B2 (ja) 2011-10-13 2011-10-13 ヒト肝細胞を担持するキメラ非ヒト動物

Publications (3)

Publication Number Publication Date
JP2013085485A JP2013085485A (ja) 2013-05-13
JP2013085485A5 JP2013085485A5 (cg-RX-API-DMAC7.html) 2014-10-30
JP5883265B2 true JP5883265B2 (ja) 2016-03-09

Family

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Family Applications (1)

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JP2011226233A Expired - Fee Related JP5883265B2 (ja) 2011-10-13 2011-10-13 ヒト肝細胞を担持するキメラ非ヒト動物

Country Status (5)

Country Link
US (1) US9420769B2 (cg-RX-API-DMAC7.html)
EP (1) EP2767160B1 (cg-RX-API-DMAC7.html)
JP (1) JP5883265B2 (cg-RX-API-DMAC7.html)
CA (1) CA2851997C (cg-RX-API-DMAC7.html)
WO (1) WO2013054949A1 (cg-RX-API-DMAC7.html)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6496107B2 (ja) * 2014-05-08 2019-04-03 株式会社フェニックスバイオ 高尿酸血症モデル
WO2018005471A1 (en) 2016-06-27 2018-01-04 Baylor College Of Medicine Human liver chimeric non-human animal with deficient p450 oxidoreductase and methods of using same
KR20220035926A (ko) * 2019-07-26 2022-03-22 앰비스 메디신즈 인코포레이티드 간세포 생성을 위한 방법 및 조성물
JP7037789B2 (ja) * 2019-12-23 2022-03-17 積水メディカル株式会社 ヒト肝細胞キメラ動物におけるヒト肝細胞置換率の測定方法
EP4408169A4 (en) * 2021-09-30 2025-10-29 Univ Southern California Human hepatocyte culture medium and conditioned medium of in vitro cultured human hepatocyte cells and their uses
CN118265448A (zh) * 2021-09-30 2024-06-28 南加州大学 人肝细胞培养基和体外培养的人肝细胞的条件化培养基及其用途

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3266766B2 (ja) 1994-08-23 2002-03-18 科学技術振興事業団 ローン性増殖能を有する肝実質細胞とその取得方法、並びにその継代培養方法
JP3211941B2 (ja) 1996-12-26 2001-09-25 科学技術振興事業団 ヒト小型肝細胞の取得方法と、この細胞の初代培養および継代培養方法
WO2001011951A1 (fr) * 1999-08-13 2001-02-22 Kirin Beer Kabushiki Kaisha Souris renfermant un cytochrome p450 humain
US6995299B2 (en) * 1999-11-02 2006-02-07 University Of Connecticut Propagation of human hepatocytes in non-human animals
US6509514B1 (en) 2000-03-17 2003-01-21 Kmt Hepatech, Inc. Chimeric animal model susceptible to human hepatitis C virus infection
JP2002045087A (ja) 2000-05-19 2002-02-12 Japan Science & Technology Corp キメラ動物
EP1496066A4 (en) * 2002-03-25 2006-09-13 Japan Science & Tech Agency ANTIBODY RECOGNIZING PROLIFERATIVE HUMAN LIVER CELLS, PROLIFERATIVE HUMAN LIVER CELLS, AND FUNCTIONAL HUMAN LIVER CELLS
WO2008001614A1 (fr) 2006-06-29 2008-01-03 Hiroshima Industrial Promotion Organization Modèle animalier de stéatohépatite non alcoolique et modèle animalier de stéatose hépatique
GB0720552D0 (en) * 2007-10-19 2007-11-28 Iti Scotland Ltd Multiple cluster knockout
JP4997544B2 (ja) * 2007-11-14 2012-08-08 国立大学法人鳥取大学 ヒトチトクロームｐ４５０遺伝子（クラスター）を含む哺乳動物人工染色体ベクター及びそれを保持する非ヒト哺乳動物
JP5557217B2 (ja) * 2010-01-06 2014-07-23 国立大学法人鳥取大学 マウス人工染色体ベクター
KR20110116817A (ko) 2010-04-20 2011-10-26 삼성전기주식회사 열전 모듈을 이용한 건축용 단열재

Also Published As

Publication number Publication date
CA2851997A1 (en) 2013-04-18
EP2767160A1 (en) 2014-08-20
WO2013054949A1 (ja) 2013-04-18
US9420769B2 (en) 2016-08-23
JP2013085485A (ja) 2013-05-13
US20140241991A1 (en) 2014-08-28
EP2767160A4 (en) 2015-07-01
CA2851997C (en) 2018-07-10
EP2767160B1 (en) 2017-01-18

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