CA2842248C - Glucoamylase variants and polynucleotides encoding same - Google Patents
Glucoamylase variants and polynucleotides encoding same Download PDFInfo
- Publication number
- CA2842248C CA2842248C CA2842248A CA2842248A CA2842248C CA 2842248 C CA2842248 C CA 2842248C CA 2842248 A CA2842248 A CA 2842248A CA 2842248 A CA2842248 A CA 2842248A CA 2842248 C CA2842248 C CA 2842248C
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- CA
- Canada
- Prior art keywords
- glucoamylase
- seq
- alpha
- amylase
- variant
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- 235000005074 zinc chloride Nutrition 0.000 description 1
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/004—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Emergency Medicine (AREA)
- Food Science & Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161531189P | 2011-09-06 | 2011-09-06 | |
| US61/531,189 | 2011-09-06 | ||
| US201161566046P | 2011-12-02 | 2011-12-02 | |
| US61/566,046 | 2011-12-02 | ||
| PCT/US2012/053779 WO2013036526A1 (en) | 2011-09-06 | 2012-09-05 | Glucoamylase variants and polynucleotides encoding same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2842248A1 CA2842248A1 (en) | 2013-03-14 |
| CA2842248C true CA2842248C (en) | 2021-02-16 |
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|---|---|---|---|
| CA2842248A Active CA2842248C (en) | 2011-09-06 | 2012-09-05 | Glucoamylase variants and polynucleotides encoding same |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9534239B2 (enExample) |
| EP (1) | EP2748315B1 (enExample) |
| CN (1) | CN103930543B (enExample) |
| CA (1) | CA2842248C (enExample) |
| DK (1) | DK2748315T3 (enExample) |
| EA (1) | EA201490568A1 (enExample) |
| ES (1) | ES2656556T3 (enExample) |
| IN (1) | IN2014CN02469A (enExample) |
| MX (1) | MX351154B (enExample) |
| WO (1) | WO2013036526A1 (enExample) |
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| CA2915336C (en) | 2013-07-17 | 2024-03-12 | Novozymes A/S | Pullulanase chimeras and polynucleotides encoding same |
| EP3712274A1 (en) | 2013-09-11 | 2020-09-23 | Novozymes A/S | Processes for producing fermentation products |
| DK3415624T3 (da) | 2014-01-22 | 2021-11-08 | Novozymes As | Pullulanasevarianter og polynukleotider, som koder for dem |
| BR112016018075B1 (pt) | 2014-02-07 | 2022-01-18 | Novozymes A/S | Composição compreendendo uma alfa-amilase, uma pululanase e uma enzima glicoamilase e método de fabricação de xarope de glicose a partir de amido liquefeito |
| ES2712402T3 (es) | 2014-06-25 | 2019-05-13 | Novozymes As | Variantes de xilanasa y polinucleótidos que las codifican |
| CN106795505A (zh) * | 2014-10-23 | 2017-05-31 | 诺维信公司 | 葡糖淀粉酶变体和编码它们的多核苷酸 |
| CA2967710A1 (en) | 2014-12-01 | 2016-06-09 | Novozymes A/S | Improved production of glucose syrups |
| WO2016205127A1 (en) | 2015-06-18 | 2016-12-22 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
| CN108699572A (zh) | 2015-12-22 | 2018-10-23 | 诺维信公司 | 使用磷脂酶c增加发酵产物得率的方法 |
| WO2018098381A1 (en) | 2016-11-23 | 2018-05-31 | Novozymes A/S | Improved yeast for ethanol production |
| BR112019018506A2 (pt) * | 2017-03-07 | 2020-04-28 | Danisco Us Inc | glucoamilase termoestável e métodos de uso da mesma |
| CA3064042A1 (en) | 2017-06-02 | 2018-12-06 | Novozymes A/S | Improved yeast for ethanol production |
| BR112019027861A2 (pt) | 2017-06-28 | 2020-09-15 | Novozymes A/S | polipeptídeo, composição, formulação de caldo integral ou composição de cultura celular, polinucleotídeo, construção de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação. |
| EP3665275A1 (en) | 2017-08-08 | 2020-06-17 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
| WO2019055455A1 (en) | 2017-09-15 | 2019-03-21 | Novozymes A/S | MIXTURES OF ENZYMES AND METHODS FOR IMPROVING THE NUTRITIONAL QUALITY OF ANIMAL FEED |
| BR112020007814A2 (pt) | 2017-10-23 | 2020-10-20 | Novozymes A/S | processos para reduzir e/ou prevenir um aumento nos níveis de ácido lático em um sistema de fermentação de biocombustível e para produção de um produto de fermentação a partir de um material contendo amido, e, uso de um polipeptídeo, ou uma composição enzimática |
| CA3089135A1 (en) | 2018-01-29 | 2019-08-01 | Novozymes A/S | Microorganisms with improved nitrogen utilization for ethanol production |
| MX2020008309A (es) | 2018-02-15 | 2020-10-14 | Novozymes As | Levadura mejorada para produccion de etanol. |
| MX2020012754A (es) | 2018-05-31 | 2021-02-26 | Novozymes As | Procesos para mejorar el crecimiento y la productividad de la levadura. |
| MX2021000112A (es) | 2018-07-11 | 2021-03-09 | Novozymes As | Procesos para producir productos de fermentacion. |
| CA3107110A1 (en) | 2018-07-25 | 2020-01-30 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
| CA3114783A1 (en) | 2018-10-08 | 2020-04-16 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
| WO2020160126A1 (en) | 2019-01-31 | 2020-08-06 | Novozymes A/S | Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed |
| MX2021015821A (es) | 2019-07-26 | 2022-02-03 | Novozymes As | Microorganismos con transporte de nitrogeno mejorado para la produccion de etanol. |
| AR119596A1 (es) | 2019-08-05 | 2021-12-29 | Novozymes As | Mezclas de enzimas y procesos para producir un ingrediente alimenticio de alto contenido proteico a partir de un subproducto de la vinaza entera |
| CN114127124A (zh) | 2019-08-06 | 2022-03-01 | 诺维信公司 | 用于提高酶表达的融合蛋白 |
| WO2021119304A1 (en) | 2019-12-10 | 2021-06-17 | Novozymes A/S | Microorganism for improved pentose fermentation |
| US20230023446A1 (en) | 2019-12-16 | 2023-01-26 | Novozymes A/S | Processes for producing fermentation products |
| MX2023014545A (es) | 2021-06-07 | 2024-03-01 | Novozymes As | Microorganismo diseñado para mejorar la fermentación del etanol. |
| CN120693401A (zh) | 2022-12-19 | 2025-09-23 | 诺维信公司 | 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途 |
| WO2024137250A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
| CN120380140A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸 |
| WO2024137704A2 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast |
| WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
| MX2025015096A (es) | 2023-06-13 | 2026-02-03 | Novozymes As | Procesos para producir productos de fermentacion usando levaduras modificadas que expresan una beta-xilosidasa |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
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| DK135983D0 (da) | 1983-03-25 | 1983-03-25 | Novo Industri As | Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| WO1989001969A1 (en) | 1987-09-04 | 1989-03-09 | Novo-Nordisk A/S | Process for the production of protein products in aspergillus and promoters for use in aspergillus |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| DK81293D0 (da) | 1993-07-06 | 1993-07-06 | Novo Nordisk As | Enzym |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| KR970703426A (ko) | 1994-06-03 | 1997-07-03 | 제임스 쉐한 | 정제된 Myceliophthora 락카제 및 그것을 암호화 하는 핵산(PURIFIED MYCELIOPHTHORA LACCASES AND NUCLEIC ACIDS ENCODING SAME) |
| EP0777737B1 (en) | 1994-06-30 | 2005-05-04 | Novozymes Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
| US6093562A (en) | 1996-02-05 | 2000-07-25 | Novo Nordisk A/S | Amylase variants |
| AR000862A1 (es) | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
| DK2199378T3 (da) | 1995-02-03 | 2012-10-29 | Novozymes As | alfa-amylase mutanter |
| EP0904360B1 (en) | 1996-04-30 | 2013-07-31 | Novozymes A/S | alpha-AMYLASE MUTANTS |
| DK2206768T3 (en) | 1997-10-13 | 2015-06-29 | Novozymes As | Alfa-amylasemutanter |
| DE69838483T2 (de) | 1997-10-14 | 2008-07-03 | Darwin Molecular Corp. | Thymidinkinase mutanten und fusionproteine mit thymidinkinase und guanylatekinase aktivitäten |
| EP1032654B1 (en) | 1997-11-26 | 2009-01-21 | Novozymes A/S | Thermostable glucoamylase |
| US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| EP1058724B1 (en) | 1998-02-27 | 2010-12-01 | Novozymes A/S | Maltogenic alpha-amylase variants |
| JP5043254B2 (ja) | 1998-10-26 | 2012-10-10 | ノボザイムス アクティーゼルスカブ | 糸状面細胞内の問題のｄｎａライブラリーの作製及びスクリーニング |
| AU4025700A (en) | 1999-03-22 | 2000-10-09 | Novo Nordisk Biotech, Inc. | Promoters for expressing genes in a fungal cell |
| CN1252256C (zh) | 1999-03-30 | 2006-04-19 | 诺维信公司 | α－淀粉酶变体 |
| US7884066B2 (en) * | 1999-10-05 | 2011-02-08 | The Regents Of The University Of California | NELL-1 enhanced bone mineralization |
| EP2180035A1 (en) | 2000-08-01 | 2010-04-28 | Novozymes A/S | Alpha-amylase mutants with altered properties |
| ATE375388T1 (de) | 2001-07-27 | 2007-10-15 | Us Gov Health & Human Serv | Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden |
| WO2003048353A1 (en) | 2001-12-07 | 2003-06-12 | Novozymes A/S | Polypeptides having protease activity and nucleic acids encoding same |
| US7785601B2 (en) * | 2002-12-31 | 2010-08-31 | Sygnis Bioscience Gmbh & Co. Kg | Methods of treating neurological conditions with hematopoietic growth factors |
| EP2166091A3 (en) | 2003-03-10 | 2015-06-10 | Novozymes A/S | Alcohol product processes |
| ES2517245T3 (es) | 2003-06-25 | 2014-11-03 | Novozymes A/S | Enzimas para el tratamiento de almidón |
| WO2005003311A2 (en) | 2003-06-25 | 2005-01-13 | Novozymes A/S | Enzymes for starch processing |
| ATE441705T1 (de) | 2003-10-30 | 2009-09-15 | Novozymes As | Kohlenhydratbindende module |
| DK1831385T3 (en) | 2004-12-22 | 2015-07-13 | Novozymes North America Inc | Enzymes for starch processing |
| EP2338905B1 (en) * | 2005-02-23 | 2017-11-29 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
| US8119384B2 (en) | 2006-06-15 | 2012-02-21 | Novozymes A/S | Process for producing a starch hydrolyzate |
| ES2668202T3 (es) | 2009-12-11 | 2018-05-17 | Novozymes A/S | Variantes de proteasa |
| MX2012007709A (es) | 2010-01-04 | 2012-08-15 | Novozymes As | Variantes de alfa-amilasa con estabilidad mejorada. |
| CA2795806C (en) | 2010-04-14 | 2019-01-08 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| CA2822637C (en) * | 2010-12-22 | 2020-06-30 | Novozymes A/S | Processes for producing fermentation products |
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- 2012-09-05 IN IN2469CHN2014 patent/IN2014CN02469A/en unknown
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- 2012-09-05 EA EA201490568A patent/EA201490568A1/ru unknown
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- 2012-09-05 DK DK12761850.2T patent/DK2748315T3/en active
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Also Published As
| Publication number | Publication date |
|---|---|
| CN103930543B (zh) | 2020-11-27 |
| EA201490568A1 (ru) | 2014-06-30 |
| US20140342422A1 (en) | 2014-11-20 |
| EP2748315B1 (en) | 2017-11-15 |
| US9534239B2 (en) | 2017-01-03 |
| MX2014002415A (es) | 2014-05-28 |
| WO2013036526A1 (en) | 2013-03-14 |
| CA2842248A1 (en) | 2013-03-14 |
| CN103930543A (zh) | 2014-07-16 |
| DK2748315T3 (en) | 2018-02-05 |
| EP2748315A1 (en) | 2014-07-02 |
| ES2656556T3 (es) | 2018-02-27 |
| MX351154B (es) | 2017-10-04 |
| IN2014CN02469A (enExample) | 2015-06-19 |
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| Date | Code | Title | Description |
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| EEER | Examination request |
Effective date: 20170804 |