CA2842248C - Glucoamylase variants and polynucleotides encoding same - Google Patents
Glucoamylase variants and polynucleotides encoding same Download PDFInfo
- Publication number
- CA2842248C CA2842248C CA2842248A CA2842248A CA2842248C CA 2842248 C CA2842248 C CA 2842248C CA 2842248 A CA2842248 A CA 2842248A CA 2842248 A CA2842248 A CA 2842248A CA 2842248 C CA2842248 C CA 2842248C
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- CA
- Canada
- Prior art keywords
- glucoamylase
- seq
- alpha
- amylase
- variant
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- 235000005074 zinc chloride Nutrition 0.000 description 1
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/004—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Emergency Medicine (AREA)
- Food Science & Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161531189P | 2011-09-06 | 2011-09-06 | |
| US61/531,189 | 2011-09-06 | ||
| US201161566046P | 2011-12-02 | 2011-12-02 | |
| US61/566,046 | 2011-12-02 | ||
| PCT/US2012/053779 WO2013036526A1 (en) | 2011-09-06 | 2012-09-05 | Glucoamylase variants and polynucleotides encoding same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2842248A1 CA2842248A1 (en) | 2013-03-14 |
| CA2842248C true CA2842248C (en) | 2021-02-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2842248A Active CA2842248C (en) | 2011-09-06 | 2012-09-05 | Glucoamylase variants and polynucleotides encoding same |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9534239B2 (en:Method) |
| EP (1) | EP2748315B1 (en:Method) |
| CN (1) | CN103930543B (en:Method) |
| CA (1) | CA2842248C (en:Method) |
| DK (1) | DK2748315T3 (en:Method) |
| EA (1) | EA201490568A1 (en:Method) |
| ES (1) | ES2656556T3 (en:Method) |
| IN (1) | IN2014CN02469A (en:Method) |
| MX (1) | MX351154B (en:Method) |
| WO (1) | WO2013036526A1 (en:Method) |
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| DK3022300T3 (en) | 2013-07-17 | 2018-10-22 | Novozymes As | : Pullulan chimeras and polynucleotides encoding them |
| CA2923934C (en) | 2013-09-11 | 2023-09-26 | Novozymes A/S | Processes for producing fermentation products |
| CA2932239C (en) | 2014-01-22 | 2023-03-14 | Novozymes A/S | Pullulanase variants and polynucleotides encoding same |
| RU2706297C2 (ru) | 2014-02-07 | 2019-11-15 | Новозимс А/С | Композиции для получения глюкозных сиропов |
| US10370682B2 (en) | 2014-06-25 | 2019-08-06 | Novozymes A/S | Xylanase variants and polynucleotides encoding same |
| US10584324B2 (en) | 2014-10-23 | 2020-03-10 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
| BR112017011275A2 (pt) | 2014-12-01 | 2018-04-03 | Novozymes A/S | composição, e, processo para a produção de xarope de glicose. |
| CA2981342A1 (en) | 2015-06-18 | 2016-12-22 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
| EP3394275A1 (en) | 2015-12-22 | 2018-10-31 | Novozymes A/S | Process of extracting oil from thin stillage |
| US10889836B2 (en) | 2016-11-23 | 2021-01-12 | Novozymes A/S | Yeast for ethanol production |
| BR112019018506A2 (pt) * | 2017-03-07 | 2020-04-28 | Danisco Us Inc | glucoamilase termoestável e métodos de uso da mesma |
| BR112019025391A2 (pt) | 2017-06-02 | 2020-07-07 | Novozymes A/S | levedura melhorada para a produção de etanol |
| ES2908190T3 (es) | 2017-06-28 | 2022-04-28 | Novozymes As | Polipéptidos con actividad de trehalasa y polinucleótidos que los codifican |
| CN111094562A (zh) | 2017-08-08 | 2020-05-01 | 诺维信公司 | 具有海藻糖酶活性的多肽及其在产生发酵产物的方法中的用途 |
| WO2019055455A1 (en) | 2017-09-15 | 2019-03-21 | Novozymes A/S | MIXTURES OF ENZYMES AND METHODS FOR IMPROVING THE NUTRITIONAL QUALITY OF ANIMAL FEED |
| BR112020007814A2 (pt) | 2017-10-23 | 2020-10-20 | Novozymes A/S | processos para reduzir e/ou prevenir um aumento nos níveis de ácido lático em um sistema de fermentação de biocombustível e para produção de um produto de fermentação a partir de um material contendo amido, e, uso de um polipeptídeo, ou uma composição enzimática |
| WO2019148192A1 (en) | 2018-01-29 | 2019-08-01 | Novozymes A/S | Microorganisms with improved nitrogen utilization for ethanol production |
| MX2020008309A (es) | 2018-02-15 | 2020-10-14 | Novozymes As | Levadura mejorada para produccion de etanol. |
| BR112020024347A2 (pt) | 2018-05-31 | 2021-02-23 | Novozymes A/S | processos para aumentar o crescimento e a produtividade de levedura |
| WO2020014407A1 (en) | 2018-07-11 | 2020-01-16 | Novozymes A/S | Processes for producing fermentation products |
| BR112021001282A2 (pt) | 2018-07-25 | 2021-04-27 | Novozymes A/S | levedura expressando enzimas para produção de etanol |
| BR112021006692A2 (pt) | 2018-10-08 | 2021-08-10 | Novozymes A/S | levedura expressando enzimas para produção de etanol |
| BR112021014873A2 (pt) | 2019-01-31 | 2021-10-05 | Novozymes A/S | Polipeptídeo, combinação de enzimas, polinucleotídeo, construto de ácido nucleico ou vetor de expressão recombinante, célula hospedeira recombinante, método de produção de um polipeptídeo, e, processo de produção de um produto de fermentação |
| EP4004029A1 (en) | 2019-07-26 | 2022-06-01 | Novozymes A/S | Microorganisms with improved nitrogen transport for ethanol production |
| MX2022000831A (es) | 2019-08-05 | 2022-02-10 | Novozymes As | Mezclas de enzimas y procesos para producir un ingrediente alimenticio de alto contenido proteico a partir de un subproducto de la vinaza entera. |
| WO2021025872A1 (en) | 2019-08-06 | 2021-02-11 | Novozymes A/S | Fusion proteins for improved enzyme expression |
| WO2021119304A1 (en) | 2019-12-10 | 2021-06-17 | Novozymes A/S | Microorganism for improved pentose fermentation |
| CN114867860A (zh) | 2019-12-16 | 2022-08-05 | 诺维信公司 | 用于生产发酵产物的方法 |
| AU2022288057A1 (en) | 2021-06-07 | 2023-12-21 | Novozymes A/S | Engineered microorganism for improved ethanol fermentation |
| WO2024137250A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
| CN120380140A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸 |
| WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
| CN120283060A (zh) | 2022-12-19 | 2025-07-08 | 诺维信公司 | 用于使用纤维降解酶和工程化酵母生产发酵产物的工艺 |
| CN120693401A (zh) | 2022-12-19 | 2025-09-23 | 诺维信公司 | 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途 |
| WO2024258820A2 (en) | 2023-06-13 | 2024-12-19 | Novozymes A/S | Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
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| DK135983D0 (da) | 1983-03-25 | 1983-03-25 | Novo Industri As | Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| DE3886221T3 (de) | 1987-09-04 | 2000-12-21 | Novo Nordisk A/S, Bagsvaerd | VERFAHREN ZUR HERSTELLUNG VON PROTEINPRODUKTEN IN -i(ASPERGILLUS) UND PROMOTOREN ZUR VERWENDUNG IN -i(ASPERGILLUS). |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| DK81293D0 (da) | 1993-07-06 | 1993-07-06 | Novo Nordisk As | Enzym |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| DK0765394T3 (da) | 1994-06-03 | 2001-12-10 | Novozymes As | Oprensede Myceliopthora-laccaser og nukleinsyrer der koder for disse |
| WO1996000787A1 (en) | 1994-06-30 | 1996-01-11 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
| KR20050046778A (ko) | 1995-02-03 | 2005-05-18 | 노보자임스 에이/에스 | 소정 특성을 가지는 알파-아밀라제 돌연변이체 |
| US6093562A (en) | 1996-02-05 | 2000-07-25 | Novo Nordisk A/S | Amylase variants |
| AR000862A1 (es) | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
| AU2692897A (en) | 1996-04-30 | 1997-11-19 | Novo Nordisk A/S | Alpha-amylase mutants |
| WO1999019467A1 (en) | 1997-10-13 | 1999-04-22 | Novo Nordisk A/S | α-AMYLASE MUTANTS |
| EP1025212B1 (en) | 1997-10-14 | 2007-09-26 | Darwin Molecular Corporation | Thymidine kinase mutants and fusion proteins having thymidine kinase and guanylate kinase activities |
| AU1434299A (en) | 1997-11-26 | 1999-06-16 | Novo Nordisk A/S | Thermostable glucoamylase |
| US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| CN1292028B (zh) | 1998-02-27 | 2013-08-14 | 诺维信公司 | 麦芽α淀粉酶变体 |
| DK1124949T3 (da) | 1998-10-26 | 2006-11-06 | Novozymes As | Konstruktion og screening af et DNA-bibliotek af interesse i trådformede svampeceller |
| EP1194572A2 (en) | 1999-03-22 | 2002-04-10 | Novozymes Biotech, Inc. | Promoter sequences derived from fusarium venenatum and uses thereof |
| DK2290060T3 (en) | 1999-03-30 | 2017-03-06 | Novozymes As | The alpha-amylase variants |
| US7884066B2 (en) * | 1999-10-05 | 2011-02-08 | The Regents Of The University Of California | NELL-1 enhanced bone mineralization |
| CA2702204C (en) | 2000-08-01 | 2011-09-06 | Novozymes A/S | Alpha-amylase mutants with altered properties |
| DE60222914T2 (de) | 2001-07-27 | 2008-07-24 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Systeme zur stellengerichteten in-vivo-mutagenese mit oligonukleotiden |
| RU2318018C2 (ru) | 2001-12-07 | 2008-02-27 | Новозимс А/С | Полипептиды, обладающие протеазной активностью, и нуклеиновые кислоты, кодирующие указанные полипептиды |
| US7785601B2 (en) * | 2002-12-31 | 2010-08-31 | Sygnis Bioscience Gmbh & Co. Kg | Methods of treating neurological conditions with hematopoietic growth factors |
| WO2004080923A2 (en) | 2003-03-10 | 2004-09-23 | Novozymes A/S | Alcohol product processes |
| JP2007526748A (ja) | 2003-06-25 | 2007-09-20 | ノボザイムス アクティーゼルスカブ | 澱粉加工用酵素 |
| ES2383366T3 (es) | 2003-06-25 | 2012-06-20 | Novozymes A/S | Enzimas para el tratamiento de almidón |
| DE602004022967D1 (de) | 2003-10-30 | 2009-10-15 | Novozymes As | Kohlenhydratbindende module |
| AU2005319073B2 (en) | 2004-12-22 | 2011-03-17 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| EP2338905B1 (en) * | 2005-02-23 | 2017-11-29 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
| PL2032713T3 (pl) | 2006-06-15 | 2010-04-30 | Novozymes As | Sposoby wytwarzania hydrolizatu skrobi |
| CN104774823B (zh) | 2009-12-11 | 2020-04-10 | 诺维信公司 | 蛋白酶变体 |
| MX2012007710A (es) | 2010-01-04 | 2012-08-15 | Novozymes As | Alfa-amilasas. |
| DK2558484T3 (en) * | 2010-04-14 | 2016-03-29 | Novozymes As | Polypeptides having glucoamylase activity and polynucleotides encoding them |
| WO2012088303A2 (en) * | 2010-12-22 | 2012-06-28 | Novozymes North America, Inc. | Processes for producing fermentation products |
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2012
- 2012-09-05 DK DK12761850.2T patent/DK2748315T3/en active
- 2012-09-05 WO PCT/US2012/053779 patent/WO2013036526A1/en active Application Filing
- 2012-09-05 EA EA201490568A patent/EA201490568A1/ru unknown
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- 2012-09-05 ES ES12761850.2T patent/ES2656556T3/es active Active
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- 2012-09-05 MX MX2014002415A patent/MX351154B/es active IP Right Grant
- 2012-09-05 CN CN201280043431.5A patent/CN103930543B/zh active Active
- 2012-09-05 IN IN2469CHN2014 patent/IN2014CN02469A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DK2748315T3 (en) | 2018-02-05 |
| EP2748315A1 (en) | 2014-07-02 |
| CN103930543A (zh) | 2014-07-16 |
| ES2656556T3 (es) | 2018-02-27 |
| EA201490568A1 (ru) | 2014-06-30 |
| WO2013036526A1 (en) | 2013-03-14 |
| IN2014CN02469A (en:Method) | 2015-06-19 |
| CN103930543B (zh) | 2020-11-27 |
| US9534239B2 (en) | 2017-01-03 |
| US20140342422A1 (en) | 2014-11-20 |
| MX2014002415A (es) | 2014-05-28 |
| CA2842248A1 (en) | 2013-03-14 |
| EP2748315B1 (en) | 2017-11-15 |
| MX351154B (es) | 2017-10-04 |
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| EEER | Examination request |
Effective date: 20170804 |