CA2842248C - Glucoamylase variants and polynucleotides encoding same - Google Patents
Glucoamylase variants and polynucleotides encoding same Download PDFInfo
- Publication number
- CA2842248C CA2842248C CA2842248A CA2842248A CA2842248C CA 2842248 C CA2842248 C CA 2842248C CA 2842248 A CA2842248 A CA 2842248A CA 2842248 A CA2842248 A CA 2842248A CA 2842248 C CA2842248 C CA 2842248C
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- CA
- Canada
- Prior art keywords
- glucoamylase
- seq
- alpha
- amylase
- variant
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- 235000005074 zinc chloride Nutrition 0.000 description 1
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12C—BEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
- C12C5/00—Other raw materials for the preparation of beer
- C12C5/004—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C13—SUGAR INDUSTRY
- C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
- C13K1/00—Glucose; Glucose-containing syrups
- C13K1/06—Glucose; Glucose-containing syrups obtained by saccharification of starch or raw materials containing starch
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Emergency Medicine (AREA)
- Food Science & Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161531189P | 2011-09-06 | 2011-09-06 | |
| US61/531,189 | 2011-09-06 | ||
| US201161566046P | 2011-12-02 | 2011-12-02 | |
| US61/566,046 | 2011-12-02 | ||
| PCT/US2012/053779 WO2013036526A1 (en) | 2011-09-06 | 2012-09-05 | Glucoamylase variants and polynucleotides encoding same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2842248A1 CA2842248A1 (en) | 2013-03-14 |
| CA2842248C true CA2842248C (en) | 2021-02-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2842248A Active CA2842248C (en) | 2011-09-06 | 2012-09-05 | Glucoamylase variants and polynucleotides encoding same |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9534239B2 (OSRAM) |
| EP (1) | EP2748315B1 (OSRAM) |
| CN (1) | CN103930543B (OSRAM) |
| CA (1) | CA2842248C (OSRAM) |
| DK (1) | DK2748315T3 (OSRAM) |
| EA (1) | EA201490568A1 (OSRAM) |
| ES (1) | ES2656556T3 (OSRAM) |
| IN (1) | IN2014CN02469A (OSRAM) |
| MX (1) | MX351154B (OSRAM) |
| WO (1) | WO2013036526A1 (OSRAM) |
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| CN105339494B (zh) | 2013-07-17 | 2020-04-17 | 诺维信公司 | 普鲁兰酶嵌合体以及编码它们的多核苷酸 |
| ES2800477T3 (es) | 2013-09-11 | 2020-12-30 | Novozymes As | Procesos para producir productos de fermentación |
| WO2015110473A2 (en) | 2014-01-22 | 2015-07-30 | Novozymes A/S | Pullulanase variants and polynucleotides encoding same |
| RU2019134734A (ru) | 2014-02-07 | 2019-11-18 | Новозимс А/С | Композиции для получения глюкозных сиропов |
| DK3161133T3 (en) | 2014-06-25 | 2019-03-25 | Novozymes As | Xylanase variants and polynucleotides encoding them |
| CN106795505A (zh) * | 2014-10-23 | 2017-05-31 | 诺维信公司 | 葡糖淀粉酶变体和编码它们的多核苷酸 |
| WO2016087445A1 (en) | 2014-12-01 | 2016-06-09 | Novozymes A/S | Improved production of glucose syrups |
| WO2016205127A1 (en) | 2015-06-18 | 2016-12-22 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
| CA3006992A1 (en) | 2015-12-22 | 2017-06-29 | Novozymes A/S | Processes for improving fermentation product yield using phospholipase c |
| WO2018098381A1 (en) | 2016-11-23 | 2018-05-31 | Novozymes A/S | Improved yeast for ethanol production |
| CA3055530A1 (en) * | 2017-03-07 | 2018-09-13 | Danisco Us Inc. | Thermostable glucoamylase and methods of use, thereof |
| MX2019014142A (es) | 2017-06-02 | 2020-02-07 | Novozymes As | Levadura mejorada para produccion de etanol. |
| CN110997701A (zh) | 2017-06-28 | 2020-04-10 | 诺维信公司 | 具有海藻糖酶活性的多肽以及编码其的多核苷酸 |
| EP3665275A1 (en) | 2017-08-08 | 2020-06-17 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
| WO2019055455A1 (en) | 2017-09-15 | 2019-03-21 | Novozymes A/S | MIXTURES OF ENZYMES AND METHODS FOR IMPROVING THE NUTRITIONAL QUALITY OF ANIMAL FEED |
| CA3075907A1 (en) | 2017-10-23 | 2019-05-02 | Novozymes A/S | Processes for reducing lactic acid in a biofuel fermentation system |
| AU2019213033A1 (en) | 2018-01-29 | 2020-09-17 | Microbiogen Pty. Ltd. | Microorganisms with improved nitrogen utilization for ethanol production |
| CA3107124A1 (en) | 2018-02-15 | 2019-08-22 | Novozymes A/S | Improved yeast for ethanol production |
| US20210198618A1 (en) | 2018-05-31 | 2021-07-01 | Novozymes A/S | Processes for enhancing yeast growth and productivity |
| US20210269833A1 (en) | 2018-07-11 | 2021-09-02 | Novozymes A/S | Processes for producing fermentation products |
| WO2020023411A1 (en) | 2018-07-25 | 2020-01-30 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
| WO2020076697A1 (en) | 2018-10-08 | 2020-04-16 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
| CN113302294A (zh) | 2019-01-31 | 2021-08-24 | 诺维信公司 | 具有木聚糖酶活性的多肽及其用于改善动物饲料营养质量的用途 |
| EP4004029A1 (en) | 2019-07-26 | 2022-06-01 | Novozymes A/S | Microorganisms with improved nitrogen transport for ethanol production |
| WO2021026201A1 (en) | 2019-08-05 | 2021-02-11 | Novozymes A/S | Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct |
| AU2020326511A1 (en) | 2019-08-06 | 2022-01-06 | Novozymes A/S | Fusion proteins for improved enzyme expression |
| AU2020402990A1 (en) | 2019-12-10 | 2022-06-09 | Novozymes A/S | Microorganism for improved pentose fermentation |
| WO2021126966A1 (en) | 2019-12-16 | 2021-06-24 | Novozymes A/S | Processes for producing fermentation products |
| WO2022261003A1 (en) | 2021-06-07 | 2022-12-15 | Novozymes A/S | Engineered microorganism for improved ethanol fermentation |
| WO2024137248A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization |
| EP4638725A1 (en) | 2022-12-19 | 2025-10-29 | Novozymes A/S | Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same |
| WO2024137252A1 (en) | 2022-12-19 | 2024-06-27 | Novozymes A/S | Process for reducing syrup viscosity in the backend of a process for producing a fermentation product |
| CN120380140A (zh) | 2022-12-19 | 2025-07-25 | 诺维信公司 | 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸 |
| CN120283060A (zh) | 2022-12-19 | 2025-07-08 | 诺维信公司 | 用于使用纤维降解酶和工程化酵母生产发酵产物的工艺 |
| AU2024303961A1 (en) | 2023-06-13 | 2025-12-11 | Novozymes A/S | Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase |
| WO2025128568A1 (en) | 2023-12-11 | 2025-06-19 | Novozymes A/S | Composition and use thereof for increasing hemicellulosic fiber solubilization |
| WO2026008449A2 (en) | 2024-07-04 | 2026-01-08 | Novozymes A/S | A process for producing a fermentation product and a concentrated protein co-product |
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| DK135983D0 (da) | 1983-03-25 | 1983-03-25 | Novo Industri As | Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse |
| DK122686D0 (da) | 1986-03-17 | 1986-03-17 | Novo Industri As | Fremstilling af proteiner |
| JP2703598B2 (ja) | 1987-09-04 | 1998-01-26 | ノボ―ノルディスク アクティーゼルスカブ | アスペルギルス菌中でのタンパク質生産物の生産方法およびアスペルギルス菌中で使用するためのプロモーター |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| IL99552A0 (en) | 1990-09-28 | 1992-08-18 | Ixsys Inc | Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| DK81293D0 (da) | 1993-07-06 | 1993-07-06 | Novo Nordisk As | Enzym |
| DE4343591A1 (de) | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| DK0765394T3 (da) | 1994-06-03 | 2001-12-10 | Novozymes As | Oprensede Myceliopthora-laccaser og nukleinsyrer der koder for disse |
| DE69534185T2 (de) | 1994-06-30 | 2006-02-23 | Novozymes Biotech, Inc., Davis | Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren |
| US6093562A (en) | 1996-02-05 | 2000-07-25 | Novo Nordisk A/S | Amylase variants |
| AU4483496A (en) | 1995-02-03 | 1996-08-21 | Novo Nordisk A/S | A method of designing alpha-amylase mutants with predetermined properties |
| AR000862A1 (es) | 1995-02-03 | 1997-08-06 | Novozymes As | Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del |
| CN1246455C (zh) | 1996-04-30 | 2006-03-22 | 诺沃奇梅兹有限公司 | α－淀粉酶变体 |
| EP2206768B1 (en) | 1997-10-13 | 2015-04-01 | Novozymes A/S | Alpha-amylase mutants |
| JP2002516061A (ja) | 1997-10-14 | 2002-06-04 | ダーウィン モレキュラー コーポレイション | チミジンキナーゼ変異体ならびにチミジンキナーゼ活性およびグアニル酸キナーゼ活性を有する融合タンパク質 |
| ES2321043T3 (es) | 1997-11-26 | 2009-06-01 | Novozymes A/S | Glucoamilasa termoestable. |
| US5955310A (en) | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| DE69942995D1 (de) | 1998-02-27 | 2011-01-13 | Novozymes As | Maltogene alpha-amylase varianten |
| EP1124949B1 (en) | 1998-10-26 | 2006-07-12 | Novozymes A/S | Constructing and screening a dna library of interest in filamentous fungal cells |
| CN1940067A (zh) | 1999-03-22 | 2007-04-04 | 诺沃奇梅兹有限公司 | 用于在真菌细胞中表达基因的启动子 |
| DK1165762T3 (da) | 1999-03-30 | 2007-09-03 | Novozymes As | Alpha-amylase varianter |
| US7884066B2 (en) * | 1999-10-05 | 2011-02-08 | The Regents Of The University Of California | NELL-1 enhanced bone mineralization |
| JP4855632B2 (ja) | 2000-08-01 | 2012-01-18 | ノボザイムス アクティーゼルスカブ | 変更された性質を有するα−アミラーゼ突然変異体 |
| EP1421187B1 (en) | 2001-07-27 | 2007-10-10 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES | Systems for in vivo site-directed mutagenesis using oligonucleotides |
| DK1456369T3 (da) | 2001-12-07 | 2010-03-22 | Novozymes As | Polypeptider med proteaseaktivitet og nukleinsyrer kodende derfor |
| US7785601B2 (en) * | 2002-12-31 | 2010-08-31 | Sygnis Bioscience Gmbh & Co. Kg | Methods of treating neurological conditions with hematopoietic growth factors |
| WO2004080923A2 (en) | 2003-03-10 | 2004-09-23 | Novozymes A/S | Alcohol product processes |
| ES2517245T3 (es) | 2003-06-25 | 2014-11-03 | Novozymes A/S | Enzimas para el tratamiento de almidón |
| WO2005003311A2 (en) | 2003-06-25 | 2005-01-13 | Novozymes A/S | Enzymes for starch processing |
| DE602004022967D1 (de) | 2003-10-30 | 2009-10-15 | Novozymes As | Kohlenhydratbindende module |
| ES2621921T3 (es) | 2004-12-22 | 2017-07-05 | Novozymes A/S | Enzimas para tratamiento de almidón |
| WO2006091194A1 (en) * | 2005-02-23 | 2006-08-31 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
| ES2336846T3 (es) | 2006-06-15 | 2010-04-16 | Novozymes A/S | Procesos para la produccion de un hidrolizado de almidon. |
| MX355362B (es) | 2009-12-11 | 2018-04-17 | Novozymes As | Variantes de proteasa. |
| EP3101127B1 (en) | 2010-01-04 | 2019-03-20 | Novozymes A/S | Alpha-amylase variants with improved stability |
| WO2011127802A1 (en) | 2010-04-14 | 2011-10-20 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
| DK2654567T3 (en) * | 2010-12-22 | 2018-06-25 | Novozymes North America Inc | Process for making fermentation products from starch-containing materials |
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2012
- 2012-09-05 CN CN201280043431.5A patent/CN103930543B/zh active Active
- 2012-09-05 EA EA201490568A patent/EA201490568A1/ru unknown
- 2012-09-05 ES ES12761850.2T patent/ES2656556T3/es active Active
- 2012-09-05 DK DK12761850.2T patent/DK2748315T3/en active
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Also Published As
| Publication number | Publication date |
|---|---|
| CN103930543B (zh) | 2020-11-27 |
| IN2014CN02469A (OSRAM) | 2015-06-19 |
| MX2014002415A (es) | 2014-05-28 |
| WO2013036526A1 (en) | 2013-03-14 |
| US9534239B2 (en) | 2017-01-03 |
| EA201490568A1 (ru) | 2014-06-30 |
| CN103930543A (zh) | 2014-07-16 |
| MX351154B (es) | 2017-10-04 |
| DK2748315T3 (en) | 2018-02-05 |
| EP2748315A1 (en) | 2014-07-02 |
| EP2748315B1 (en) | 2017-11-15 |
| CA2842248A1 (en) | 2013-03-14 |
| ES2656556T3 (es) | 2018-02-27 |
| US20140342422A1 (en) | 2014-11-20 |
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| EEER | Examination request |
Effective date: 20170804 |