CA2841824C - Bacterial host strain expressing recombinant dsbc - Google Patents

Bacterial host strain expressing recombinant dsbc Download PDF

Info

Publication number
CA2841824C
CA2841824C CA2841824A CA2841824A CA2841824C CA 2841824 C CA2841824 C CA 2841824C CA 2841824 A CA2841824 A CA 2841824A CA 2841824 A CA2841824 A CA 2841824A CA 2841824 C CA2841824 C CA 2841824C
Authority
CA
Canada
Prior art keywords
seq
antibody
protein
gene
gram
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2841824A
Other languages
English (en)
French (fr)
Other versions
CA2841824A1 (en
Inventor
Mark Ellis
David Paul Humphreys
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UCB SA
Original Assignee
UCB SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UCB SA filed Critical UCB SA
Publication of CA2841824A1 publication Critical patent/CA2841824A1/en
Application granted granted Critical
Publication of CA2841824C publication Critical patent/CA2841824C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2875Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the NGF/TNF superfamily, e.g. CD70, CD95L, CD153, CD154
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90Isomerases (5.)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
CA2841824A 2011-07-13 2012-07-13 Bacterial host strain expressing recombinant dsbc Active CA2841824C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11173880.3 2011-07-13
EP11173880 2011-07-13
PCT/EP2012/002945 WO2013007388A1 (en) 2011-07-13 2012-07-13 Bacterial host strain expressing recombinant dsbc

Publications (2)

Publication Number Publication Date
CA2841824A1 CA2841824A1 (en) 2013-01-17
CA2841824C true CA2841824C (en) 2021-07-13

Family

ID=46545736

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2841824A Active CA2841824C (en) 2011-07-13 2012-07-13 Bacterial host strain expressing recombinant dsbc

Country Status (27)

Country Link
US (2) US9725516B2 (OSRAM)
EP (2) EP3339325A1 (OSRAM)
JP (3) JP6431370B2 (OSRAM)
KR (1) KR102023786B1 (OSRAM)
CN (1) CN103649124B (OSRAM)
AU (1) AU2012283388B2 (OSRAM)
BR (1) BR112013032871B1 (OSRAM)
CA (1) CA2841824C (OSRAM)
CY (1) CY1119985T1 (OSRAM)
DK (1) DK2731973T3 (OSRAM)
EA (1) EA031449B1 (OSRAM)
ES (1) ES2659155T3 (OSRAM)
HR (1) HRP20180226T1 (OSRAM)
HU (1) HUE035674T2 (OSRAM)
IL (1) IL229947B (OSRAM)
IN (1) IN2014DN00202A (OSRAM)
LT (1) LT2731973T (OSRAM)
ME (1) ME02957B (OSRAM)
MX (1) MX348738B (OSRAM)
NO (1) NO2731973T3 (OSRAM)
PL (1) PL2731973T3 (OSRAM)
PT (1) PT2731973T (OSRAM)
RS (1) RS56926B1 (OSRAM)
SG (1) SG10201807560XA (OSRAM)
SI (1) SI2731973T1 (OSRAM)
SM (1) SMT201800068T1 (OSRAM)
WO (1) WO2013007388A1 (OSRAM)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUE041818T2 (hu) * 2007-03-22 2019-05-28 Biogen Ma Inc Kötõ proteinek, beleértve az antitesteket, CD154-et specifikusan kötõ antitest származékokat és antitest fragmenseket is, és alkalmazásaik
LT2993231T (lt) 2009-09-24 2018-11-12 Ucb Biopharma Sprl Rekombinantinio baltymo raiškai skirtas bakterijų kamienas, turintis proteazės negaminantį degp su išlaikytų šaperoniniu aktyvumu ir išveiklintus tsp ir ptr genus
GB201000591D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000587D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000590D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
US9725516B2 (en) * 2011-07-13 2017-08-08 Ucb Pharma, S.A. Bacterial host strain expressing recombinant DSBC
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
GB201223276D0 (en) * 2012-12-21 2013-02-06 Ucb Pharma Sa Antibodies and methods of producing same
WO2016102383A1 (en) 2014-12-22 2016-06-30 Ucb Biopharma Sprl Protein manufacture
KR101810778B1 (ko) * 2015-06-23 2017-12-20 서울대학교산학협력단 Cd154 결합 폴리펩타이드 및 그 용도
CN108495653A (zh) 2016-01-27 2018-09-04 免疫医疗有限责任公司 用于制备具有定义的糖基化模式抗体的方法
US11453883B2 (en) 2018-04-05 2022-09-27 Bio-Rad Abd Serotec Gmbh Display systems for proteins of interest
CA3117051A1 (en) * 2018-11-05 2020-05-14 Genentech, Inc. Methods of producing two chain proteins in prokaryotic host cells
CN109371049A (zh) * 2018-11-14 2019-02-22 天津大学 分子伴侣在促进单克隆抗体的形成和/或提高单克隆抗体的表达量中的应用
CN113874518B (zh) 2019-03-18 2025-04-25 生物辐射Abd瑟罗泰克有限公司 保护含SpyTag的周质融合蛋白免于蛋白酶Tsp和OmpT降解
DK4010500T3 (da) * 2019-08-05 2025-05-05 Wacker Chemie Ag Bakteriestamme til frigivelse af et rekombinant protein i en fermenteringsfremgangsmåde

Family Cites Families (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8422238D0 (en) 1984-09-03 1984-10-10 Neuberger M S Chimeric proteins
GB8907617D0 (en) 1989-04-05 1989-05-17 Celltech Ltd Drug delivery system
US5264365A (en) 1990-11-09 1993-11-23 Board Of Regents, The University Of Texas System Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides
US5508192A (en) 1990-11-09 1996-04-16 Board Of Regents, The University Of Texas System Bacterial host strains for producing proteolytically sensitive polypeptides
GB9112536D0 (en) 1991-06-11 1991-07-31 Celltech Ltd Chemical compounds
US5474771A (en) 1991-11-15 1995-12-12 The Trustees Of Columbia University In The City Of New York Murine monoclonal antibody (5c8) recognizes a human glycoprotein on the surface of T-lymphocytes, compositions containing same
EP0640093A4 (en) 1992-04-28 1996-06-05 Univ Michigan HUMAN CRABP-I AND CRABP-II.
GB9215540D0 (en) 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5304472A (en) 1992-11-20 1994-04-19 Genentech, Inc. Method of controlling polypeptide production in bacterial cells
US5639635A (en) 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
US6027888A (en) 1996-04-05 2000-02-22 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells
GB9625640D0 (en) 1996-12-10 1997-01-29 Celltech Therapeutics Ltd Biological products
US6083715A (en) 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
JP2000083670A (ja) 1998-09-09 2000-03-28 Hsp Kenkyusho:Kk DsbA/DsbB/DsbC/DsbD発現プラスミド
AU767670B2 (en) 1999-06-29 2003-11-20 Siga Technologies, Inc. DEGP periplasmic protease a new anti-infective target and an invitro assay for DEGP protease function
GB0006398D0 (en) 2000-03-16 2000-05-03 Novartis Ag Organic compounds
GB0013810D0 (en) 2000-06-06 2000-07-26 Celltech Chiroscience Ltd Biological products
EP1314037A2 (en) 2000-09-01 2003-05-28 Biogen, Inc. Methods of designing and producing compounds having improved binding affinity for cd154 or other trimeric proteins
AU8867501A (en) 2000-09-01 2002-03-13 Biogen Inc Co-crystal structure of monoclonal antibody 5c8 and cd154, and use thereof in drug design
US7041479B2 (en) 2000-09-06 2006-05-09 The Board Of Trustess Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
CA2430182C (en) * 2000-12-14 2011-01-25 Genentech, Inc. Prokaryotically produced antibodies and uses thereof
DK1341899T3 (da) 2000-12-14 2006-04-10 Genentech Inc Bakterieværtsstammer
TWI334439B (en) 2001-08-01 2010-12-11 Centocor Inc Anti-tnf antibodies, compositions, methods and uses
KR100968664B1 (ko) 2001-08-27 2010-07-06 제넨테크, 인크. 항체 발현 및 조립을 위한 시스템
GB0124317D0 (en) 2001-10-10 2001-11-28 Celltech R&D Ltd Biological products
EP2022799A2 (en) 2001-11-16 2009-02-11 Biogen Idec Inc. Polycistronic expression of antibodies
GB0129105D0 (en) 2001-12-05 2002-01-23 Celltech R&D Ltd Expression control using variable intergenic sequences
EP2135879A3 (en) 2002-06-28 2010-06-23 Domantis Limited Ligand
US7608429B2 (en) 2002-10-31 2009-10-27 Genentech, Inc. Methods and compositions for increasing antibody production
JP4603894B2 (ja) 2002-12-03 2010-12-22 ユセベ ファルマ ソシエテ アノニム 抗体産生細胞を同定するためのアッセイ
GB0303337D0 (en) 2003-02-13 2003-03-19 Celltech R&D Ltd Biological products
NZ544486A (en) 2003-06-13 2009-04-30 Biogen Idec Inc Aglycosyl anti-cd154 (cd40 ligand) antibodies and uses thereof
DK1639011T3 (da) 2003-06-30 2009-02-16 Domantis Ltd Pegylerede enkelt-domæne antistoffer (dAb)
GB0315457D0 (en) 2003-07-01 2003-08-06 Celltech R&D Ltd Biological products
GB0315450D0 (en) 2003-07-01 2003-08-06 Celltech R&D Ltd Biological products
CN1894695A (zh) 2003-07-26 2007-01-10 比奥根艾迪克Ma公司 具有改良的抗原结合亲和力的改变的抗体
KR20070047327A (ko) 2004-07-26 2007-05-04 비오겐 아이덱 엠에이 아이엔씨. 항-cd154 항체
JP2008511337A (ja) * 2004-09-02 2008-04-17 ジェネンテック・インコーポレーテッド ヘテロ多量体分子
US7563443B2 (en) 2004-09-17 2009-07-21 Domantis Limited Monovalent anti-CD40L antibody polypeptides and compositions thereof
GB0425534D0 (en) 2004-11-19 2004-12-22 Celltech R&D Ltd Process for obtaining antibodies
GB0520169D0 (en) 2005-10-04 2005-11-09 Celltech R&D Ltd Biological products
HUE041818T2 (hu) 2007-03-22 2019-05-28 Biogen Ma Inc Kötõ proteinek, beleértve az antitesteket, CD154-et specifikusan kötõ antitest származékokat és antitest fragmenseket is, és alkalmazásaik
CA2700714C (en) 2007-09-26 2018-09-11 Ucb Pharma S.A. Dual specificity antibody fusions
US7662587B1 (en) 2009-03-05 2010-02-16 E. I. Du Pont De Nemours And Company Gene knockout mutations that increase peptide production
LT2993231T (lt) 2009-09-24 2018-11-12 Ucb Biopharma Sprl Rekombinantinio baltymo raiškai skirtas bakterijų kamienas, turintis proteazės negaminantį degp su išlaikytų šaperoniniu aktyvumu ir išveiklintus tsp ir ptr genus
US8470552B2 (en) 2009-10-12 2013-06-25 Keck Graduate Institute Strategy to reduce lactic acid production and control PH in animal cell culture
KR101968766B1 (ko) * 2009-11-05 2019-04-12 제넨테크, 인크. 이종 폴리펩티드의 분비를 위한 방법 및 조성물
GB201000587D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000588D0 (en) * 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201000590D0 (en) * 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201000591D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201001791D0 (en) 2010-02-03 2010-03-24 Ucb Pharma Sa Process for obtaining antibodies
GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
EP2546267A1 (en) 2011-07-13 2013-01-16 UCB Pharma S.A. Bacterial host strain expressing recombinant DsbC
US9725516B2 (en) * 2011-07-13 2017-08-08 Ucb Pharma, S.A. Bacterial host strain expressing recombinant DSBC
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product

Also Published As

Publication number Publication date
JP2018019719A (ja) 2018-02-08
KR102023786B1 (ko) 2019-09-20
US9725516B2 (en) 2017-08-08
EP2731973A1 (en) 2014-05-21
BR112013032871B1 (pt) 2022-09-27
WO2013007388A1 (en) 2013-01-17
US20140141468A1 (en) 2014-05-22
BR112013032871A8 (pt) 2022-08-23
SMT201800068T1 (it) 2018-03-08
CN103649124B (zh) 2016-04-13
HK1194393A1 (zh) 2014-10-17
ME02957B (me) 2018-07-20
CN103649124A (zh) 2014-03-19
JP2020028299A (ja) 2020-02-27
JP2014521313A (ja) 2014-08-28
EA031449B1 (ru) 2019-01-31
JP7062626B2 (ja) 2022-05-17
KR20140039240A (ko) 2014-04-01
US9957328B2 (en) 2018-05-01
EP3339325A1 (en) 2018-06-27
PT2731973T (pt) 2018-02-22
IL229947B (en) 2019-10-31
ES2659155T3 (es) 2018-03-14
LT2731973T (lt) 2018-02-26
CY1119985T1 (el) 2018-12-12
SG10201807560XA (en) 2018-10-30
DK2731973T3 (da) 2018-01-29
SI2731973T1 (en) 2018-04-30
EP2731973B1 (en) 2017-11-15
CA2841824A1 (en) 2013-01-17
NO2731973T3 (OSRAM) 2018-04-14
PL2731973T3 (pl) 2018-04-30
AU2012283388A1 (en) 2014-01-09
AU2012283388B2 (en) 2017-04-20
EA201490274A1 (ru) 2014-05-30
HUE035674T2 (en) 2018-05-28
US20170335003A1 (en) 2017-11-23
RS56926B1 (sr) 2018-05-31
MX2014000302A (es) 2014-02-17
MX348738B (es) 2017-06-27
IN2014DN00202A (OSRAM) 2015-06-05
HRP20180226T1 (hr) 2018-03-09
BR112013032871A2 (pt) 2017-01-24
JP6431370B2 (ja) 2018-11-28

Similar Documents

Publication Publication Date Title
US9957328B2 (en) Bacterial host strain expressing recombinant DSBC
US9493559B2 (en) Bacterial host strain expressing recombinant DsbC and having reduced Tsp activity
US8969039B2 (en) Bacterial host strain comprising a mutant SPR gene and having reduced TSP activity
US9493558B2 (en) Bacterial host strain comprising a mutant SPR gene and a wild-type TSP gene
HK1194393B (en) Bacterial host strain expressing recombinant dsbc
BR112012016808B1 (pt) Cepa hospedeira bacteriana expressando dsbc recombinante e tendo atividade tsp reduzida

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20170516