CA2820094C - Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides - Google Patents

Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides Download PDF

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Publication number
CA2820094C
CA2820094C CA2820094A CA2820094A CA2820094C CA 2820094 C CA2820094 C CA 2820094C CA 2820094 A CA2820094 A CA 2820094A CA 2820094 A CA2820094 A CA 2820094A CA 2820094 C CA2820094 C CA 2820094C
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nucleic acid
fluorophore
inhibitor
target nucleic
oligonucleotide
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CA2820094A
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English (en)
French (fr)
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CA2820094A1 (en
Inventor
Tal Raz
Pascaline Mary
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Optics & Photonics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Dispersion Chemistry (AREA)
CA2820094A 2010-12-07 2011-12-07 Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides Active CA2820094C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42074710P 2010-12-07 2010-12-07
US61/420,747 2010-12-07
PCT/US2011/063654 WO2012078710A1 (en) 2010-12-07 2011-12-07 Nucleic acid target detection using a detector, a probe and an inhibitor

Publications (2)

Publication Number Publication Date
CA2820094A1 CA2820094A1 (en) 2012-06-14
CA2820094C true CA2820094C (en) 2019-02-26

Family

ID=46207491

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2820094A Active CA2820094C (en) 2010-12-07 2011-12-07 Nucleic acid target detection using fluorophore- and quencher-conjugated oligonucleotides

Country Status (8)

Country Link
US (2) US9581549B2 (cg-RX-API-DMAC7.html)
EP (1) EP2649202B1 (cg-RX-API-DMAC7.html)
JP (1) JP6096672B2 (cg-RX-API-DMAC7.html)
CN (2) CN103348017B (cg-RX-API-DMAC7.html)
AU (1) AU2011338502B2 (cg-RX-API-DMAC7.html)
CA (1) CA2820094C (cg-RX-API-DMAC7.html)
SG (1) SG191725A1 (cg-RX-API-DMAC7.html)
WO (1) WO2012078710A1 (cg-RX-API-DMAC7.html)

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EP2649202B1 (en) 2010-12-07 2019-02-20 Bio-Rad Laboratories, Inc. Nucleic acid target detection using a detector, a probe and an inhibitor
CN103765068B (zh) 2011-03-30 2016-09-07 努拜欧有限公司 将多个体积注入或注出液滴
US9816931B2 (en) 2011-03-31 2017-11-14 Bio-Rad Laboratories, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
US9228898B2 (en) 2011-03-31 2016-01-05 Gnubio, Inc. Scalable spectroscopic detection and measurement
EP2895591A4 (en) 2012-09-12 2016-10-12 Gnubio Inc INTEGRATED MICROFLUIDIC SYSTEM, METHOD AND KIT FOR PERFORMING TESTS
US9592503B2 (en) 2013-01-25 2017-03-14 Gnubio, Inc. System and method for performing droplet inflation
EP2989213B1 (en) 2013-04-26 2019-11-06 Bio-Rad Laboratories, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
US9766261B2 (en) 2013-05-29 2017-09-19 Bio-Rad Laboratories, Inc. Low cost optical high speed discrete measurement system
EP3004391B1 (en) * 2013-05-29 2019-03-27 Bio-Rad Laboratories, Inc. Methods for sequencing in emulsion based microfluidics
WO2015031528A1 (en) 2013-08-27 2015-03-05 Gnubio, Inc. Microfluidic devices and methods of their use
CN105636697B (zh) 2013-09-30 2018-06-12 基纽拜奥股份有限公司 微流体盒装置和使用方法以及组件
EP3074122A4 (en) 2013-11-27 2017-11-29 Bio-Rad Laboratories, Inc. Microfluidic droplet packing
CN106573245B (zh) 2014-06-30 2019-06-18 生物辐射实验室股份有限公司 实现pcr的浮动热接触
US11118217B2 (en) 2017-01-30 2021-09-14 Bio-Rad Laboratories, Inc. Emulsion compositions and methods of their use
WO2018175399A1 (en) 2017-03-24 2018-09-27 Bio-Rad Laboratories, Inc. Universal hairpin primers

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EP2989213B1 (en) 2013-04-26 2019-11-06 Bio-Rad Laboratories, Inc. A method for blocking polymerase extension of 3 prime dna ends by stem-loop structure
EP3004391B1 (en) 2013-05-29 2019-03-27 Bio-Rad Laboratories, Inc. Methods for sequencing in emulsion based microfluidics
US9766261B2 (en) 2013-05-29 2017-09-19 Bio-Rad Laboratories, Inc. Low cost optical high speed discrete measurement system
WO2015031528A1 (en) 2013-08-27 2015-03-05 Gnubio, Inc. Microfluidic devices and methods of their use
CN105636697B (zh) 2013-09-30 2018-06-12 基纽拜奥股份有限公司 微流体盒装置和使用方法以及组件
CN106573245B (zh) 2014-06-30 2019-06-18 生物辐射实验室股份有限公司 实现pcr的浮动热接触

Also Published As

Publication number Publication date
CN105603071A (zh) 2016-05-25
CA2820094A1 (en) 2012-06-14
SG191725A1 (en) 2013-08-30
WO2012078710A1 (en) 2012-06-14
CN105603071B (zh) 2019-06-21
EP2649202B1 (en) 2019-02-20
US20170226568A1 (en) 2017-08-10
US10533212B2 (en) 2020-01-14
US9581549B2 (en) 2017-02-28
US20130344485A1 (en) 2013-12-26
EP2649202A4 (en) 2015-05-27
AU2011338502A1 (en) 2013-06-13
JP2014500729A (ja) 2014-01-16
CN103348017B (zh) 2016-01-20
EP2649202A1 (en) 2013-10-16
CN103348017A (zh) 2013-10-09
JP6096672B2 (ja) 2017-03-15
AU2011338502B2 (en) 2016-08-11

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