CA2807688A1 - Food additive comprising amidase for detoxifying ochratoxin - Google Patents
Food additive comprising amidase for detoxifying ochratoxin Download PDFInfo
- Publication number
- CA2807688A1 CA2807688A1 CA2807688A CA2807688A CA2807688A1 CA 2807688 A1 CA2807688 A1 CA 2807688A1 CA 2807688 A CA2807688 A CA 2807688A CA 2807688 A CA2807688 A CA 2807688A CA 2807688 A1 CA2807688 A1 CA 2807688A1
- Authority
- CA
- Canada
- Prior art keywords
- seq
- amidase
- sequence
- ochratoxin
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- 102000005922 amidase Human genes 0.000 title claims abstract description 165
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- 239000002778 food additive Substances 0.000 title claims abstract description 59
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- RWQKHEORZBHNRI-BMIGLBTASA-N ochratoxin A Chemical compound C([C@H](NC(=O)C1=CC(Cl)=C2C[C@H](OC(=O)C2=C1O)C)C(O)=O)C1=CC=CC=C1 RWQKHEORZBHNRI-BMIGLBTASA-N 0.000 claims description 234
- VYLQGYLYRQKMFU-UHFFFAOYSA-N Ochratoxin A Natural products CC1Cc2c(Cl)cc(CNC(Cc3ccccc3)C(=O)O)cc2C(=O)O1 VYLQGYLYRQKMFU-UHFFFAOYSA-N 0.000 claims description 229
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- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 241000228158 x Triticosecale Species 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 229910052727 yttrium Inorganic materials 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
- C12Y305/01004—Amidase (3.5.1.4)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Nutrition Science (AREA)
- Animal Husbandry (AREA)
- Medicinal Chemistry (AREA)
- Physiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Fodder In General (AREA)
- General Preparation And Processing Of Foods (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38028010P | 2010-09-06 | 2010-09-06 | |
US61/380,280 | 2010-09-06 | ||
EP10177410 | 2010-09-17 | ||
EP10177410.7 | 2010-09-17 | ||
PCT/IB2011/053901 WO2012032472A2 (en) | 2010-09-06 | 2011-09-06 | Additive |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2807688A1 true CA2807688A1 (en) | 2012-03-15 |
Family
ID=43501087
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2807688A Abandoned CA2807688A1 (en) | 2010-09-06 | 2011-09-06 | Food additive comprising amidase for detoxifying ochratoxin |
Country Status (12)
Country | Link |
---|---|
US (1) | US9113649B2 (es) |
EP (1) | EP2613647B1 (es) |
JP (1) | JP2013540430A (es) |
CN (1) | CN103209597B (es) |
AR (1) | AR087928A1 (es) |
AU (1) | AU2011300392A1 (es) |
BR (1) | BR112013005287B1 (es) |
CA (1) | CA2807688A1 (es) |
DK (1) | DK2613647T3 (es) |
ES (1) | ES2664178T3 (es) |
PL (1) | PL2613647T3 (es) |
WO (1) | WO2012032472A2 (es) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113693189A (zh) * | 2021-09-28 | 2021-11-26 | 中国农业科学院北京畜牧兽医研究所 | 锰过氧化物酶用于降解棒曲霉素的应用及方法 |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2016104064A (ru) | 2013-07-10 | 2017-08-15 | Новартис Аг | Клетки мицелиальных грибов с множественной недостаточностью протеаз и способы их использования |
CN103937681B (zh) * | 2014-04-11 | 2016-06-29 | 江南大学 | 一株食品级黑曲霉及其在玉米赤霉烯酮降解的应用 |
US10513724B2 (en) | 2014-07-21 | 2019-12-24 | Glykos Finland Oy | Production of glycoproteins with mammalian-like N-glycans in filamentous fungi |
CN104783021B (zh) * | 2015-04-15 | 2018-05-25 | 甘肃农业大学 | 以环糊精为吸附剂脱除t-2毒素的方法 |
CN105833459B (zh) * | 2016-04-26 | 2019-01-11 | 中国农业科学院农产品加工研究所 | 铜绿假单胞菌在降解赭曲霉毒素中的应用 |
CN108251399B (zh) * | 2016-12-29 | 2020-08-21 | 中粮营养健康研究院有限公司 | 伏马毒素降解酶、编码基因、重组载体、细胞、添加剂及其应用 |
CN108244348B (zh) * | 2016-12-29 | 2021-07-06 | 中粮营养健康研究院有限公司 | 酶复合制剂、饲料或添加剂以及脱除呕吐毒素的方法 |
CN108251405B (zh) * | 2016-12-29 | 2020-08-21 | 中粮营养健康研究院有限公司 | 复合酶和添加剂及它们的应用以及脱除真菌毒素的方法 |
CN108251385B (zh) * | 2016-12-29 | 2020-11-17 | 中粮营养健康研究院有限公司 | 赭曲霉毒素降解酶、编码基因、重组载体、细胞、添加剂及其应用 |
KR102005434B1 (ko) * | 2018-02-22 | 2019-07-30 | 강원대학교산학협력단 | 아플라톡신 생성 아스퍼질러스 플라버스 균주를 억제하는 바실러스 서틸리스 af11 |
CN112218953A (zh) * | 2018-03-09 | 2021-01-12 | 丹尼斯科美国公司 | 葡糖淀粉酶及其使用方法 |
CN108385439A (zh) * | 2018-04-20 | 2018-08-10 | 张剑 | 一种造纸制浆专用树脂控制剂 |
CN109439640B (zh) * | 2018-10-09 | 2021-07-20 | 天津科技大学 | 伏马毒素降解酶FumDSB及其基因和应用 |
CN109439639B (zh) * | 2018-10-09 | 2021-07-20 | 天津科技大学 | 伏马毒素降解酶FumDPS及其基因和应用 |
CN110172425B (zh) * | 2019-05-30 | 2020-12-04 | 华中农业大学 | 玉米赤霉烯酮脱毒型益生菌的筛选及应用 |
CN110495549B (zh) * | 2019-07-05 | 2022-04-22 | 中国农业大学 | 一种β-葡聚糖酶及其在抑制赭曲霉中的应用 |
CN112430611A (zh) * | 2020-11-30 | 2021-03-02 | 华南理工大学 | 一种经优化的玉米赤霉烯酮降解酶zhd-p编码基因、重组菌体、表面展示体系与应用 |
WO2022129517A2 (en) | 2020-12-18 | 2022-06-23 | Erber Aktiengesellschaft | Means and methods for detoxifying ochratoxin a |
WO2022236165A1 (en) * | 2021-05-07 | 2022-11-10 | The Better Meat Co. | Enhanced aerobic fermentation methods for producing edible fungal mycelium blended meats and meat analogue compositions |
KR20240040123A (ko) | 2021-08-12 | 2024-03-27 | 안카 안게반테 카페테크놀로기 게엠베하 | 식료품 및 고급식품에서 아크릴아미드를 제거하는 방법 |
AR126884A1 (es) | 2021-08-27 | 2023-11-22 | DSM Austria GmbH | Medios y métodos para detoxificar ocratoxina a |
WO2023148301A1 (en) | 2022-02-02 | 2023-08-10 | Biotalys NV | Modified microbial cells and uses thereof |
CN116286384A (zh) * | 2022-12-15 | 2023-06-23 | 中国农业科学院农产品加工研究所 | 褐色绿僵菌ifst-ot3及其产生的脱毒酶与应用 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6426410B1 (en) | 1998-12-22 | 2002-07-30 | Genencor International, Inc. | Phenol oxidizing enzymes |
JP2000226335A (ja) * | 1998-12-04 | 2000-08-15 | Amano Pharmaceut Co Ltd | 経口用酵素製剤、酵素含有食材及び酵素製剤の服用方法 |
US20020081352A1 (en) * | 2000-12-22 | 2002-06-27 | Rhode Rodger R. | Enzyme food supplement composition comprising lipase and lactase |
AT413540B (de) * | 2001-12-20 | 2006-03-15 | Erber Ag | Mikroorganismus, welcher ochratoxine sowie ochratoxine und zearalenone entgiftet, sowie verfahren und verwendung hiefür |
CA2771875A1 (en) | 2003-04-01 | 2005-01-06 | Danisco Us Inc. | Variant hypocrea jecorina cbh1 |
CN101040046A (zh) * | 2004-10-15 | 2007-09-19 | 帝斯曼知识产权资产管理有限公司 | 新颖的食物制造方法 |
EA014853B1 (ru) * | 2004-10-15 | 2011-02-28 | ДСМ АйПи АССЕТС Б.В. | Амидаза из aspergillus niger и применение амидазы для получения пищевого продукта с пониженным содержанием акриламида |
MY148594A (en) | 2005-10-12 | 2013-05-15 | Genencor Int | Stable, durable granules with active agents |
EP2458007A1 (en) * | 2005-11-29 | 2012-05-30 | DSM IP Assets B.V. | DNA binding site of a transcriptional activator useful in gene expression |
US20110294191A1 (en) | 2008-07-29 | 2011-12-01 | Danisco Us Inc. | Increased production of aspartic proteases in filamentous fungal cells |
US20100057531A1 (en) | 2008-09-03 | 2010-03-04 | International Business Machines Corporation | Discovering Rarely-Planned Parts using Order Proposal Data |
JP2012528598A (ja) | 2009-06-03 | 2012-11-15 | ダニスコ・ユーエス・インク | 改善された発現、活性、及び安定性を有するセルラーゼ変異体、並びにこれらの使用方法 |
-
2011
- 2011-09-06 CA CA2807688A patent/CA2807688A1/en not_active Abandoned
- 2011-09-06 CN CN201180042549.1A patent/CN103209597B/zh not_active Expired - Fee Related
- 2011-09-06 ES ES11767471.3T patent/ES2664178T3/es active Active
- 2011-09-06 AU AU2011300392A patent/AU2011300392A1/en not_active Abandoned
- 2011-09-06 EP EP11767471.3A patent/EP2613647B1/en not_active Not-in-force
- 2011-09-06 WO PCT/IB2011/053901 patent/WO2012032472A2/en active Application Filing
- 2011-09-06 PL PL11767471T patent/PL2613647T3/pl unknown
- 2011-09-06 JP JP2013526594A patent/JP2013540430A/ja active Pending
- 2011-09-06 BR BR112013005287A patent/BR112013005287B1/pt not_active IP Right Cessation
- 2011-09-06 US US13/820,971 patent/US9113649B2/en active Active
- 2011-09-06 AR ARP110103252A patent/AR087928A1/es unknown
- 2011-09-06 DK DK11767471.3T patent/DK2613647T3/en active
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113693189A (zh) * | 2021-09-28 | 2021-11-26 | 中国农业科学院北京畜牧兽医研究所 | 锰过氧化物酶用于降解棒曲霉素的应用及方法 |
CN113693189B (zh) * | 2021-09-28 | 2023-11-17 | 中国农业科学院北京畜牧兽医研究所 | 锰过氧化物酶用于降解棒曲霉素的应用及方法 |
Also Published As
Publication number | Publication date |
---|---|
WO2012032472A2 (en) | 2012-03-15 |
PL2613647T3 (pl) | 2018-07-31 |
AR087928A1 (es) | 2014-04-30 |
BR112013005287A2 (pt) | 2017-05-02 |
ES2664178T3 (es) | 2018-04-18 |
BR112013005287B1 (pt) | 2020-05-05 |
AU2011300392A1 (en) | 2013-02-28 |
CN103209597B (zh) | 2016-06-29 |
US9113649B2 (en) | 2015-08-25 |
US20130216654A1 (en) | 2013-08-22 |
WO2012032472A3 (en) | 2012-05-03 |
EP2613647B1 (en) | 2018-02-14 |
DK2613647T3 (en) | 2018-05-22 |
CN103209597A (zh) | 2013-07-17 |
JP2013540430A (ja) | 2013-11-07 |
EP2613647A2 (en) | 2013-07-17 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |
Effective date: 20160830 |
|
FZDE | Discontinued |
Effective date: 20230220 |
|
FZDE | Discontinued |
Effective date: 20230220 |