CA2805247C - Nanopore-facilitated single molecule detection of nucleic acids - Google Patents

Nanopore-facilitated single molecule detection of nucleic acids Download PDF

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Publication number
CA2805247C
CA2805247C CA2805247A CA2805247A CA2805247C CA 2805247 C CA2805247 C CA 2805247C CA 2805247 A CA2805247 A CA 2805247A CA 2805247 A CA2805247 A CA 2805247A CA 2805247 C CA2805247 C CA 2805247C
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probe
nanopore
oligonucleotide
target
mir
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English (en)
French (fr)
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CA2805247A1 (en
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Li-qun GU
Yong Wang
Kai TIAN
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University of Missouri St Louis
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University of Missouri St Louis
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CA2805247A 2010-07-14 2011-07-14 Nanopore-facilitated single molecule detection of nucleic acids Active CA2805247C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US39957810P 2010-07-14 2010-07-14
US61/399,578 2010-07-14
PCT/US2011/044082 WO2012009578A2 (en) 2010-07-14 2011-07-14 Nanopore-facilitated single molecule detection of nucleic acids

Publications (2)

Publication Number Publication Date
CA2805247A1 CA2805247A1 (en) 2012-01-19
CA2805247C true CA2805247C (en) 2021-08-10

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CA2805247A Active CA2805247C (en) 2010-07-14 2011-07-14 Nanopore-facilitated single molecule detection of nucleic acids

Country Status (6)

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US (3) US9395353B2 (enExample)
EP (2) EP3190193A1 (enExample)
JP (2) JP6016792B2 (enExample)
AU (1) AU2011279083B2 (enExample)
CA (1) CA2805247C (enExample)
WO (1) WO2012009578A2 (enExample)

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Publication number Publication date
EP2593568A4 (en) 2014-03-05
JP2013540423A (ja) 2013-11-07
EP2593568B1 (en) 2017-04-19
AU2011279083A1 (en) 2013-01-31
US20170002403A1 (en) 2017-01-05
US20130220809A1 (en) 2013-08-29
WO2012009578A2 (en) 2012-01-19
JP6016792B2 (ja) 2016-10-26
US10273527B2 (en) 2019-04-30
CA2805247A1 (en) 2012-01-19
JP2017006135A (ja) 2017-01-12
US9395353B2 (en) 2016-07-19
EP3190193A1 (en) 2017-07-12
WO2012009578A3 (en) 2012-04-05
EP2593568A2 (en) 2013-05-22
US9574228B2 (en) 2017-02-21
AU2011279083B2 (en) 2015-03-26
US20160319333A1 (en) 2016-11-03
JP6521916B2 (ja) 2019-05-29

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