AU2011279083B2 - Nanopore-facilitated single molecule detection of nucleic acids - Google Patents

Nanopore-facilitated single molecule detection of nucleic acids Download PDF

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Publication number
AU2011279083B2
AU2011279083B2 AU2011279083A AU2011279083A AU2011279083B2 AU 2011279083 B2 AU2011279083 B2 AU 2011279083B2 AU 2011279083 A AU2011279083 A AU 2011279083A AU 2011279083 A AU2011279083 A AU 2011279083A AU 2011279083 B2 AU2011279083 B2 AU 2011279083B2
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probe
nanopore
mir
target
oligonucleotide
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AU2011279083A1 (en
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Li-qun GU
Kai TIAN
Yong Wang
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University of Missouri St Louis
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University of Missouri Columbia
University of Missouri St Louis
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
AU2011279083A 2010-07-14 2011-07-14 Nanopore-facilitated single molecule detection of nucleic acids Active AU2011279083B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US39957810P 2010-07-14 2010-07-14
US61/399,578 2010-07-14
PCT/US2011/044082 WO2012009578A2 (en) 2010-07-14 2011-07-14 Nanopore-facilitated single molecule detection of nucleic acids

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AU2011279083A1 AU2011279083A1 (en) 2013-01-31
AU2011279083B2 true AU2011279083B2 (en) 2015-03-26

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US (3) US9395353B2 (enExample)
EP (2) EP3190193A1 (enExample)
JP (2) JP6016792B2 (enExample)
AU (1) AU2011279083B2 (enExample)
CA (1) CA2805247C (enExample)
WO (1) WO2012009578A2 (enExample)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101495656B (zh) 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 采用带修饰的核苷酸通过纳米通道进行dna序列测定
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9194838B2 (en) 2010-03-03 2015-11-24 Osaka University Method and device for identifying nucleotide, and method and device for determining nucleotide sequence of polynucleotide
WO2012009578A2 (en) * 2010-07-14 2012-01-19 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
ES2641871T3 (es) 2010-12-17 2017-11-14 The Trustees Of Columbia University In The City Of New York Secuenciación de ADN mediante síntesis usando nucleótidos modificados y detección con nanoporos
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
US9581563B2 (en) 2011-01-24 2017-02-28 Genia Technologies, Inc. System for communicating information from an array of sensors
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
WO2013109970A1 (en) * 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
US10739341B2 (en) 2012-02-15 2020-08-11 Oxford Nanopore Technologies Limited Aptamer method
ES2732256T3 (es) * 2012-02-16 2019-11-21 Genia Tech Inc Procedimientos para crear bicapas para su uso con sensores con nanoporos
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
EP2836604B1 (en) 2012-04-09 2021-09-15 The Trustees of Columbia University in the City of New York Method of preparation of nanopore and uses thereof
WO2013188841A1 (en) 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
CA2880274A1 (en) 2012-08-03 2014-02-06 University Of Washington Through Its Center For Commercialization Compositions and methods for improving nanopore sequencing
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US10732183B2 (en) 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
JP2016517275A (ja) * 2013-03-15 2016-06-16 ザ キュレイターズ オブ ザ ユニバーシティ オブ ミズーリ 多重核酸検出のためのコード化ナノポアセンサー
EP3014264A4 (en) 2013-06-25 2016-11-16 Two Pore Guys Inc QUANTIFICATION OF MULTIPLEXED BIOMARKERS BY NANOPORENT ANALYSIS OF BIOMARKER POLYMER COMPLEXES
EP3047282B1 (en) 2013-09-18 2019-05-15 Quantum Biosystems Inc. Biomolecule sequencing devices, systems and methods
JP6334118B2 (ja) * 2013-09-24 2018-05-30 クオンタムバイオシステムズ株式会社 単分子識別方法、装置、及びプログラム
JP2015077652A (ja) 2013-10-16 2015-04-23 クオンタムバイオシステムズ株式会社 ナノギャップ電極およびその製造方法
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
JP6461943B2 (ja) 2013-10-23 2019-01-30 ジェニア・テクノロジーズ・インコーポレイテッド ナノポアを備えた高速分子検知
CN106715453B (zh) 2014-03-24 2021-04-30 哥伦比亚大学董事会 用于生产带标签的核苷酸的化学方法
US10438811B1 (en) 2014-04-15 2019-10-08 Quantum Biosystems Inc. Methods for forming nano-gap electrodes for use in nanosensors
WO2015170782A1 (en) 2014-05-08 2015-11-12 Osaka University Devices, systems and methods for linearization of polymers
CN106796214B (zh) * 2014-07-31 2020-01-14 伊鲁米那股份有限公司 混合纳米孔传感器
EP4397972A3 (en) * 2014-10-16 2024-10-09 Oxford Nanopore Technologies PLC Alignment mapping estimation
KR102457147B1 (ko) 2014-10-17 2022-10-19 옥스포드 나노포어 테크놀로지즈 피엘씨 나노 세공 rna 특징 규명을 위한 방법
CN105842453B (zh) * 2015-01-12 2018-12-25 南京理工大学 一种基于末端延伸、金纳米粒子和酶三级放大电化学传感器检测dna的方法
CA2986921A1 (en) 2015-06-02 2016-12-08 Nanopore Diagnostics, Llc Nucleic acid detection
JP6889701B2 (ja) * 2015-09-24 2021-06-18 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ アルファ溶血素バリアント
WO2017167811A1 (en) * 2016-03-31 2017-10-05 Genia Technologies, Inc. Nanopore protein conjugates and uses thereof
ES2877193T3 (es) 2016-04-27 2021-11-16 Quantum Biosystems Inc Sistemas y métodos para la medición y secuenciación de biomoléculas
US10317392B2 (en) 2016-06-23 2019-06-11 Roche Sequencing Solutions, Inc. Formation and calibration of nanopore sequencing cells
US11124827B2 (en) 2016-06-23 2021-09-21 Roche Sequencing Solutions, Inc. Period-to-period analysis of AC signals from nanopore sequencing
EP3828280A1 (en) * 2016-07-12 2021-06-02 Rijksuniversiteit Groningen Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin
WO2018093976A1 (en) * 2016-11-16 2018-05-24 Nanopore Diagnostics, Llc Analysis of nucleic acids using probe with non-linear tag
CN116087307B (zh) * 2017-07-17 2025-10-17 哈佛大学校董委员会 用于分子表征的纳米孔匹配的蛋白质穿梭物及其数据分析方法
CN111512155B (zh) 2017-12-28 2022-07-05 豪夫迈·罗氏有限公司 测量和去除来自交流信号驱动的纳米孔dna测序系统的随机信号中的噪声
US11034996B2 (en) 2018-03-02 2021-06-15 The Curators Of The University Of Missouri Nucleic acid sequence and capture by formation of an abasic site-derived cross-link
WO2019173799A1 (en) * 2018-03-08 2019-09-12 Caris Science, Inc. Oligonucleotide probes and uses thereof
JP7239965B2 (ja) * 2018-06-26 2023-03-15 国立大学法人東京農工大学 ２種以上の特定オリゴヌクレオチドの有無を判定する判定方法、並びに、前記判定方法を用いて検査対象が小細胞肺がん、胆管がんに罹患している又はそのリスクを有することを判定する方法
US20220162692A1 (en) * 2018-07-30 2022-05-26 Oxford University Innovation Limited Assemblies
US11162935B2 (en) 2018-08-24 2021-11-02 University Of Notre Dame Du Lac Systems and methods for separating, detecting, and quantifying a target polynucleotide
CA3111488A1 (en) 2018-09-11 2020-03-19 Rijksuniversiteit Groningen Biological nanopores having tunable pore diameters and uses thereof as analytical tools
CN112280875B (zh) * 2019-07-22 2023-07-18 四川大学华西医院 一种利用纳米孔进行细菌耐药性的快速检测方法、装置和系统
KR102390925B1 (ko) * 2019-11-01 2022-04-27 한국생명공학연구원 나노포어를 이용한 병원성 미생물의 검출 방법 및 검출키트
WO2021231920A1 (en) * 2020-05-15 2021-11-18 The Curators Of The University Of Missouri Nanopore unzipping-sequencing for dna data storage
JP7679055B2 (ja) * 2020-08-24 2025-05-19 国立大学法人東京農工大学 極低濃度のオリゴヌクレオチドの存在の有無を判定する方法、及び検査対象が特定疾患に罹患しているかどうか又は特定疾患罹患のリスクを有するかどうかを判定する方法
CN112795628B (zh) * 2021-02-05 2024-02-23 南京邮电大学 基于固态纳米孔传感器的miRNA检测方法、检测探针及试剂盒
CN112961906B (zh) * 2021-02-05 2024-01-23 南京邮电大学 金纳米棒检测探针、制备方法、检测方法及其应用
CN113189070B (zh) * 2021-04-28 2024-01-16 香港理工大学 一种纳米探针及其制备方法和应用、循环肿瘤细胞的miRNAs的检测系统
IL320502A (en) 2022-10-28 2025-06-01 Univ Groningen Nanopore-based protein efflux

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
AU8586298A (en) 1997-07-25 1999-02-16 University Of Massachusetts Designed protein pores as components for biosensors
CA2366289A1 (en) 1999-03-25 2000-09-28 Radoje T. Drmanac Solution-based methods and materials for sequence analysis by hybridization
CA2384100C (en) 1999-09-07 2011-03-29 The Regents Of The University Of California Methods of determining the presence of double stranded nucleic acids in a sample
US6916665B2 (en) 2000-02-11 2005-07-12 The Texas A&M University System Biosensor compositions and methods of use
WO2003000920A2 (en) 2001-06-21 2003-01-03 President And Fellows Of Harvard College Methods for characterization of nucleic acid molecules
AU2002365421A1 (en) 2001-07-10 2003-09-02 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for detecting the activation state of the multiple proteins in single cells
US20050019784A1 (en) 2002-05-20 2005-01-27 Xing Su Method and apparatus for nucleic acid sequencing and identification
WO2005013901A2 (en) 2003-07-31 2005-02-17 Isis Pharmaceuticals, Inc. Oligomeric compounds and compositions for use in modulation of small non-coding rnas
US7846738B2 (en) 2003-08-15 2010-12-07 President And Fellows Of Harvard College Study of polymer molecules and conformations with a nanopore
US20050136408A1 (en) 2003-12-19 2005-06-23 May Tom-Moy Methods and systems for characterizing a polymer
CN102925549A (zh) 2004-08-13 2013-02-13 哈佛学院院长等 超高处理量光学-纳米孔dna读出平台
KR101159072B1 (ko) 2005-01-20 2012-06-25 삼성전자주식회사 나노포어를 이용한 생분자의 분리방법
KR100707198B1 (ko) 2005-06-27 2007-04-13 삼성전자주식회사 나노포어 및 핵산에 비특이적으로 결합하는 물질을 이용한고감도 핵산 검출 방법
US20070190542A1 (en) 2005-10-03 2007-08-16 Ling Xinsheng S Hybridization assisted nanopore sequencing
KR100730350B1 (ko) 2005-10-17 2007-06-19 삼성전자주식회사 표면처리된 나노포어를 이용한 ｄｎａ 검출방법 및검출장치
WO2007120265A2 (en) 2005-11-14 2007-10-25 Applera Corporation Coded molecules for detecting target analytes
WO2007117832A2 (en) 2006-03-12 2007-10-18 Applera Corporation Methods of detecting target nucleic acids
US8314220B2 (en) 2007-01-26 2012-11-20 Agilent Technologies, Inc. Methods compositions, and kits for detection of microRNA
WO2008097190A1 (en) 2007-02-05 2008-08-14 Agency For Science, Technology And Research Methods of electrically detecting a nucleic acid by means of an electrode pair
US8500982B2 (en) 2007-04-04 2013-08-06 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
GB0713143D0 (en) 2007-07-06 2007-08-15 Ucl Business Plc Nucleic acid detection method
JP5309145B2 (ja) 2007-10-02 2013-10-09 プレジデント アンド フェロウズ オブ ハーバード カレッジ ナノポアによる分子の捕捉、再捕捉およびトラッピング
US20090181390A1 (en) 2008-01-11 2009-07-16 Signosis, Inc. A California Corporation High throughput detection of micrornas and use for disease diagnosis
WO2009092035A2 (en) * 2008-01-17 2009-07-23 Sequenom, Inc. Methods and compositions for the analysis of biological molecules
ATE530497T1 (de) 2008-03-31 2011-11-15 Sony Deutschland Gmbh Verfahren zur herstellung einer membran mit konischer pore
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
US20100099198A1 (en) 2008-07-11 2010-04-22 Board Of Regents, The University Of Texas System Apparatus and system for pattern recognition sensing for biomolecules
WO2010062903A2 (en) 2008-11-26 2010-06-03 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
US20110003703A1 (en) 2009-07-01 2011-01-06 Charles Ma Nucleic Acid Hybridization and Detection Using Enzymatic Reactions on a Microarray
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
WO2011028494A2 (en) 2009-08-24 2011-03-10 Trustees Of Boston University Label-free sensing of pna-dna complexes using nanopores
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
EP2537026B1 (en) * 2010-02-19 2018-06-27 The Trustees Of The University Of Pennsylvania High-resolution analysis devices and related methods
US20130203610A1 (en) 2010-03-30 2013-08-08 Trustees Of Boston University Tools and Method for Nanopores Unzipping-Dependent Nucleic Acid Sequencing
WO2012009578A2 (en) 2010-07-14 2012-01-19 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
ES2641871T3 (es) 2010-12-17 2017-11-14 The Trustees Of Columbia University In The City Of New York Secuenciación de ADN mediante síntesis usando nucleótidos modificados y detección con nanoporos
US10739341B2 (en) 2012-02-15 2020-08-11 Oxford Nanopore Technologies Limited Aptamer method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Nakane, J. et al., Biophysical Journal. 2004 vol. 87 pages 615-621 *
Wang, Y. et al., Biophysical Journal. January 2010 vol. 98 no. 3 supp. 1 pages 599A-600A. Abstract 3108-Pos *

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EP2593568A4 (en) 2014-03-05
JP2013540423A (ja) 2013-11-07
EP2593568B1 (en) 2017-04-19
AU2011279083A1 (en) 2013-01-31
US20170002403A1 (en) 2017-01-05
US20130220809A1 (en) 2013-08-29
WO2012009578A2 (en) 2012-01-19
JP6016792B2 (ja) 2016-10-26
CA2805247C (en) 2021-08-10
US10273527B2 (en) 2019-04-30
CA2805247A1 (en) 2012-01-19
JP2017006135A (ja) 2017-01-12
US9395353B2 (en) 2016-07-19
EP3190193A1 (en) 2017-07-12
WO2012009578A3 (en) 2012-04-05
EP2593568A2 (en) 2013-05-22
US9574228B2 (en) 2017-02-21
US20160319333A1 (en) 2016-11-03
JP6521916B2 (ja) 2019-05-29

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