CA2790153C - Thd primer target detection - Google Patents

Thd primer target detection Download PDF

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Publication number
CA2790153C
CA2790153C CA2790153A CA2790153A CA2790153C CA 2790153 C CA2790153 C CA 2790153C CA 2790153 A CA2790153 A CA 2790153A CA 2790153 A CA2790153 A CA 2790153A CA 2790153 C CA2790153 C CA 2790153C
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CA
Canada
Prior art keywords
nucleic acid
primer
target nucleic
acid sequence
thd
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2790153A
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English (en)
French (fr)
Other versions
CA2790153A1 (en
Inventor
Jong Yoon Chun
In Taek Hwang
Young Jo Lee
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Seegene Inc
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Seegene Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/101—Taqman
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels
    • C12Q2565/1015—Interaction between at least two labels labels being on the same oligonucleotide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2790153A 2009-11-07 2009-11-28 Thd primer target detection Active CA2790153C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR10-2009-0107262 2009-11-07
KR1020090107262A KR20110050327A (ko) 2009-11-07 2009-11-07 Ｔｈｄ 프라이머 타겟 검출
PCT/KR2009/007064 WO2011055875A1 (en) 2009-11-07 2009-11-28 Thd primer target detection

Publications (2)

Publication Number Publication Date
CA2790153A1 CA2790153A1 (en) 2011-05-12
CA2790153C true CA2790153C (en) 2017-07-11

Family

ID=43970103

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2790153A Active CA2790153C (en) 2009-11-07 2009-11-28 Thd primer target detection

Country Status (9)

Country Link
US (1) US9328377B2 (enExample)
EP (1) EP2496709B1 (enExample)
JP (1) JP5770738B2 (enExample)
KR (2) KR20110050327A (enExample)
CN (1) CN102782150B (enExample)
AU (1) AU2009355009B8 (enExample)
CA (1) CA2790153C (enExample)
ES (1) ES2533793T3 (enExample)
WO (1) WO2011055875A1 (enExample)

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WO2011027966A2 (en) * 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
KR101590175B1 (ko) * 2009-09-24 2016-01-29 주식회사 씨젠 반복적 엑소핵산 절단 반응에 의한 타겟 핵산서열의 검출
US9845492B2 (en) * 2009-12-21 2017-12-19 Seegene, Inc. TSG primer target detection
KR101552669B1 (ko) * 2009-12-24 2015-09-11 주식회사 씨젠 오류 시그널이 배제되는 타겟 핵산서열의 실시간 멀티플렉싱 검출
US9074249B2 (en) 2012-06-04 2015-07-07 New England Biolabs, Inc. Detection of amplification products
US9074243B2 (en) 2012-07-27 2015-07-07 New England Biolabs, Inc. Detection of amplification products
JP6544861B2 (ja) * 2013-02-07 2019-07-17 ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー 高い選択性の核酸増幅プライマー
US20180073082A1 (en) * 2015-02-25 2018-03-15 Diogene Co., Ltd Dumbbell-structure oligonucleotide, nucleic acid amplification primer comprising same, and nucleic acid amplification method using same
CN105368943B (zh) * 2015-11-21 2018-10-16 中国人民解放军第三〇九医院 一种用于鉴定分枝杆菌菌种的试剂盒及方法
EP3397765B1 (en) * 2015-12-30 2023-02-01 Bio-Rad Laboratories, Inc. Method for quantitating the frequency of wild-type and mutant fragments in a nucleic acid sample
CN107937607B (zh) * 2017-11-30 2020-12-01 东北农业大学 用于猪传染性胃肠炎病毒检测的dpo引物组、含有该引物组的试剂盒及其应用
CN108060269B (zh) * 2018-01-19 2020-12-01 东北农业大学 用于猪流行性腹泻病毒、猪传染性胃肠炎病毒以及猪轮状病毒检测的dpo引物组及其应用
WO2022061593A1 (zh) * 2020-09-23 2022-03-31 迈克生物股份有限公司 检测核酸的组合、方法及试剂盒
WO2024054924A1 (en) * 2022-09-08 2024-03-14 Gen-Probe Incorporated Method of detecting nucleic acid analytes using dual-specificity primers
KR20260035366A (ko) * 2024-09-05 2026-03-13 에스디바이오센서 주식회사 실시간 pcr용 조성물

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
IL86724A (en) 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
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Also Published As

Publication number Publication date
KR101404130B1 (ko) 2014-06-10
AU2009355009B8 (en) 2014-05-22
KR20110050327A (ko) 2011-05-13
CN102782150B (zh) 2015-04-01
AU2009355009B2 (en) 2014-05-08
CA2790153A1 (en) 2011-05-12
KR20120084320A (ko) 2012-07-27
JP2013509871A (ja) 2013-03-21
ES2533793T3 (es) 2015-04-14
EP2496709A1 (en) 2012-09-12
JP5770738B2 (ja) 2015-08-26
US20120219955A1 (en) 2012-08-30
CN102782150A (zh) 2012-11-14
US9328377B2 (en) 2016-05-03
EP2496709B1 (en) 2015-01-07
WO2011055875A1 (en) 2011-05-12
AU2009355009A8 (en) 2014-05-22
AU2009355009A1 (en) 2012-05-31
EP2496709A4 (en) 2013-03-27

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