CA2780767A1 - Beta-glucosidase i variants with improved properties - Google Patents
Beta-glucosidase i variants with improved properties Download PDFInfo
- Publication number
- CA2780767A1 CA2780767A1 CA2780767A CA2780767A CA2780767A1 CA 2780767 A1 CA2780767 A1 CA 2780767A1 CA 2780767 A CA2780767 A CA 2780767A CA 2780767 A CA2780767 A CA 2780767A CA 2780767 A1 CA2780767 A1 CA 2780767A1
- Authority
- CA
- Canada
- Prior art keywords
- bgl1
- iota
- wild type
- over wild
- variant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108010047754 beta-Glucosidase Proteins 0.000 title claims abstract description 64
- 102000006995 beta-Glucosidase Human genes 0.000 title claims abstract description 63
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- 241001304302 Kuraishia capsulata Species 0.000 description 1
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- 102220465530 Lymphocyte activation gene 3 protein_R125A_mutation Human genes 0.000 description 1
- 239000004145 Methyl glucoside-coconut oil ester Substances 0.000 description 1
- 101100179449 Nicotiana tabacum A622 gene Proteins 0.000 description 1
- 241000787361 Parastagonospora avenae Species 0.000 description 1
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- 102220603072 Transcription factor SOX-4_W37A_mutation Human genes 0.000 description 1
- 102220474750 Tryptophan-tRNA ligase, cytoplasmic_R363A_mutation Human genes 0.000 description 1
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- 108010091371 endoglucanase 1 Proteins 0.000 description 1
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- 108010092450 endoglucanase Z Proteins 0.000 description 1
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
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- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
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- 108010083879 xyloglucan endo(1-4)-beta-D-glucanase Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US26324009P | 2009-11-20 | 2009-11-20 | |
| US61/263,240 | 2009-11-20 | ||
| PCT/US2010/057531 WO2011063308A2 (en) | 2009-11-20 | 2010-11-19 | Beta-glucosidase i variants with improved properties |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2780767A1 true CA2780767A1 (en) | 2011-05-26 |
Family
ID=43608030
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2780767A Abandoned CA2780767A1 (en) | 2009-11-20 | 2010-11-19 | Beta-glucosidase i variants with improved properties |
Country Status (10)
Families Citing this family (54)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102791853B (zh) | 2009-09-23 | 2015-08-12 | 丹尼斯科美国公司 | 新型糖基水解酶及其用途 |
| WO2011066457A2 (en) | 2009-11-25 | 2011-06-03 | Codexis, Inc. | Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass |
| RU2012131405A (ru) | 2009-12-23 | 2014-01-27 | ДАНИСКО ЮЭс ИНК. | Способы улучшения эффективности одновременно протекающих реакций осахаривания и ферментации |
| US10130697B2 (en) | 2010-03-23 | 2018-11-20 | Wisconsin Alumni Research Foundation (Warf) | Vaccines comprising mutant attenuated influenza viruses |
| EP3023492B1 (en) | 2010-10-01 | 2018-01-24 | Novozymes, Inc. | Beta-glucosidase variants and polynucleotides encoding same |
| WO2012060389A1 (ja) * | 2010-11-05 | 2012-05-10 | 旭硝子株式会社 | シゾサッカロミセス属酵母の形質転換体およびその製造方法 |
| KR20140017612A (ko) | 2011-03-17 | 2014-02-11 | 다니스코 유에스 인크. | 당화 공정에서 점도를 감소시키는 방법 |
| CN102816748B (zh) * | 2011-06-09 | 2014-07-16 | 中国科学院上海生命科学研究院 | 一种新型β-葡萄糖苷酶及其编码基因和应用 |
| ES2655051T3 (es) | 2011-08-26 | 2018-02-16 | Wisconsin Alumni Research Foundation | Virus de la gripe con segmento génico PB2 mutante como vacunas vivas atenuadas |
| EP2929021A1 (en) | 2012-12-07 | 2015-10-14 | Danisco US Inc. | Compositions and methods of use |
| ES2521940B1 (es) | 2013-05-10 | 2015-09-09 | Abengoa Bioenergía Nuevas Tecnologías, S.A. | Variantes de beta-glucosidasa con actividad de transglicosilación reducida |
| US10081802B2 (en) | 2013-07-29 | 2018-09-25 | Danisco Us Inc. | Variant Enzymes |
| WO2015042064A1 (en) * | 2013-09-17 | 2015-03-26 | Edeniq, Inc. | Improved beta-glucosidase enzymes for increased biomass saccharification |
| WO2015128417A1 (en) * | 2014-02-26 | 2015-09-03 | Chr. Hansen A/S | Variants of chymosin with improved milk-clotting properties |
| WO2015196150A2 (en) | 2014-06-20 | 2015-12-23 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
| JP2017524363A (ja) * | 2014-08-08 | 2017-08-31 | ザイレコ，インコーポレイテッド | アグリコシル化された酵素およびその使用 |
| US20170362621A1 (en) | 2014-12-18 | 2017-12-21 | Danisco Us Inc. | Engineered multifunctional enzymes and methods of use |
| EP3234118A1 (en) | 2014-12-18 | 2017-10-25 | Danisco US Inc. | Engineered multifunctional enzymes and methods of use |
| WO2016100871A1 (en) * | 2014-12-19 | 2016-06-23 | Danisco Us Inc | Glucoamylase blends |
| WO2017007907A1 (en) | 2015-07-07 | 2017-01-12 | Danisco Us Inc. | Induction of gene expression using a high concentration sugar mixture |
| EP3702469A1 (en) * | 2015-12-10 | 2020-09-02 | DSM IP Assets B.V. | Beta-glucosidase and uses thereof |
| EP3387140A1 (en) * | 2015-12-10 | 2018-10-17 | DSM IP Assets B.V. | Beta-glucosidase and uses thereof |
| WO2017097897A1 (en) * | 2015-12-10 | 2017-06-15 | Dsm Ip Assets B.V. | Beta-glucosidase and uses thereof |
| EP3417056A1 (en) | 2016-02-19 | 2018-12-26 | Wisconsin Alumni Research Foundation (WARF) | Improved influenza b virus replication for vaccine development |
| EP3336180A1 (en) * | 2016-07-04 | 2018-06-20 | Evonik Degussa GmbH | Mutant alkb gene |
| WO2018053058A1 (en) | 2016-09-14 | 2018-03-22 | Danisco Us Inc. | Lignocellulosic biomass fermentation-based processes |
| CN109790510B (zh) | 2016-10-04 | 2024-04-12 | 丹尼斯科美国公司 | 在不存在诱导底物下丝状真菌细胞中的蛋白产生 |
| WO2018106656A1 (en) | 2016-12-06 | 2018-06-14 | Danisco Us Inc | Truncated lpmo enzymes and use thereof |
| RU2771261C2 (ru) | 2016-12-21 | 2022-04-29 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Способы применения термостабильных сериновых протеаз |
| WO2018164876A1 (en) | 2017-03-06 | 2018-09-13 | Dupont Nutrition Biosciences Aps | Novel fungal fucosidases and their use in preventing and/or treating a pathogenic infection in an animal |
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- 2010-11-19 CN CN201080061975.5A patent/CN102844429B/zh not_active Expired - Fee Related
- 2010-11-19 IN IN3825DEN2012 patent/IN2012DN03825A/en unknown
- 2010-11-19 EP EP12199327.3A patent/EP2599861B1/en not_active Not-in-force
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- 2010-11-19 JP JP2012540120A patent/JP2013511280A/ja active Pending
- 2010-11-19 DK DK12199328.1T patent/DK2602317T3/da active
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