CA2767929A1 - Procede d'obtention de myofibroblastes - Google Patents

Procede d'obtention de myofibroblastes Download PDF

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Publication number
CA2767929A1
CA2767929A1 CA2767929A CA2767929A CA2767929A1 CA 2767929 A1 CA2767929 A1 CA 2767929A1 CA 2767929 A CA2767929 A CA 2767929A CA 2767929 A CA2767929 A CA 2767929A CA 2767929 A1 CA2767929 A1 CA 2767929A1
Authority
CA
Canada
Prior art keywords
cells
myofibroblasts
serum
culture
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2767929A
Other languages
English (en)
French (fr)
Inventor
Nicolas Jacques Rouyer
Robert Barthel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ONCOBIOTEK
Original Assignee
ONCOBIOTEK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ONCOBIOTEK filed Critical ONCOBIOTEK
Publication of CA2767929A1 publication Critical patent/CA2767929A1/fr
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0656Adult fibroblasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/11Epidermal growth factor [EGF]

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2767929A 2009-07-24 2010-07-02 Procede d'obtention de myofibroblastes Abandoned CA2767929A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0955216A FR2948381B1 (fr) 2009-07-24 2009-07-24 Procede d'obtention de myofibroblastes.
FR0955216 2009-07-24
PCT/EP2010/059478 WO2011009706A1 (fr) 2009-07-24 2010-07-02 Procede d'obtention de myofibroblastes

Publications (1)

Publication Number Publication Date
CA2767929A1 true CA2767929A1 (fr) 2011-01-27

Family

ID=41460947

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2767929A Abandoned CA2767929A1 (fr) 2009-07-24 2010-07-02 Procede d'obtention de myofibroblastes

Country Status (12)

Country Link
US (1) US9243228B2 (enrdf_load_stackoverflow)
EP (1) EP2456854B1 (enrdf_load_stackoverflow)
JP (1) JP5758891B2 (enrdf_load_stackoverflow)
CN (1) CN102471761A (enrdf_load_stackoverflow)
AU (1) AU2010275678B2 (enrdf_load_stackoverflow)
CA (1) CA2767929A1 (enrdf_load_stackoverflow)
ES (1) ES2650664T3 (enrdf_load_stackoverflow)
FR (1) FR2948381B1 (enrdf_load_stackoverflow)
IL (1) IL217352A (enrdf_load_stackoverflow)
MX (1) MX2012001004A (enrdf_load_stackoverflow)
NZ (1) NZ597728A (enrdf_load_stackoverflow)
WO (1) WO2011009706A1 (enrdf_load_stackoverflow)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104096265B (zh) * 2014-07-01 2016-03-23 温州医科大学 一种用于构建人工血管模型的三维收缩模型的制备方法
CN104630134A (zh) * 2015-02-15 2015-05-20 叶中华 阴道上皮细胞培养基及阴道上皮细胞培养方法
CN111500527A (zh) * 2020-06-30 2020-08-07 北京昱龙盛世生物科技有限公司 一种表皮干细胞的分离培养方法
WO2023039371A1 (en) * 2021-09-07 2023-03-16 Wisconsin Alumni Research Foundation Cardiac fibroblast derived extracellular matrix
CN115197892A (zh) * 2022-06-13 2022-10-18 浙江省人民医院 一种人甲状腺乳头状癌组织中成纤维细胞的分离培养方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573937A (en) * 1989-12-07 1996-11-12 Snow Brand Milk Products Co., Ltd. Serum free culture medium
WO2005078070A1 (ja) * 2004-02-13 2005-08-25 Reprocell, Inc. 胚性幹細胞用フィーダー細胞作成培地およびフィーダー細胞
JP5523674B2 (ja) * 2005-02-11 2014-06-18 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 植物タンパク質加水分解産物を含有する血清フリーの細胞培養液におけるポリペプチドの生産

Also Published As

Publication number Publication date
EP2456854A1 (fr) 2012-05-30
US20120190109A1 (en) 2012-07-26
AU2010275678A1 (en) 2012-02-09
MX2012001004A (es) 2012-05-29
WO2011009706A1 (fr) 2011-01-27
IL217352A (en) 2016-03-31
IL217352A0 (en) 2012-02-29
FR2948381A1 (fr) 2011-01-28
EP2456854B1 (fr) 2017-08-30
FR2948381B1 (fr) 2013-07-12
JP2013500003A (ja) 2013-01-07
CN102471761A (zh) 2012-05-23
US9243228B2 (en) 2016-01-26
NZ597728A (en) 2013-11-29
ES2650664T3 (es) 2018-01-19
JP5758891B2 (ja) 2015-08-05
AU2010275678B2 (en) 2016-06-16

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20150630

FZDE Discontinued

Effective date: 20190703