CA2767929A1 - Procede d'obtention de myofibroblastes - Google Patents
Procede d'obtention de myofibroblastes Download PDFInfo
- Publication number
- CA2767929A1 CA2767929A1 CA2767929A CA2767929A CA2767929A1 CA 2767929 A1 CA2767929 A1 CA 2767929A1 CA 2767929 A CA2767929 A CA 2767929A CA 2767929 A CA2767929 A CA 2767929A CA 2767929 A1 CA2767929 A1 CA 2767929A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- myofibroblasts
- serum
- culture
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0656—Adult fibroblasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
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- Chemical & Material Sciences (AREA)
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- Rheumatology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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FR0955216A FR2948381B1 (fr) | 2009-07-24 | 2009-07-24 | Procede d'obtention de myofibroblastes. |
FR0955216 | 2009-07-24 | ||
PCT/EP2010/059478 WO2011009706A1 (fr) | 2009-07-24 | 2010-07-02 | Procede d'obtention de myofibroblastes |
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CA2767929A1 true CA2767929A1 (fr) | 2011-01-27 |
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CA2767929A Abandoned CA2767929A1 (fr) | 2009-07-24 | 2010-07-02 | Procede d'obtention de myofibroblastes |
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CN104096265B (zh) * | 2014-07-01 | 2016-03-23 | 温州医科大学 | 一种用于构建人工血管模型的三维收缩模型的制备方法 |
CN104630134A (zh) * | 2015-02-15 | 2015-05-20 | 叶中华 | 阴道上皮细胞培养基及阴道上皮细胞培养方法 |
CN111500527A (zh) * | 2020-06-30 | 2020-08-07 | 北京昱龙盛世生物科技有限公司 | 一种表皮干细胞的分离培养方法 |
WO2023039371A1 (en) * | 2021-09-07 | 2023-03-16 | Wisconsin Alumni Research Foundation | Cardiac fibroblast derived extracellular matrix |
CN115197892A (zh) * | 2022-06-13 | 2022-10-18 | 浙江省人民医院 | 一种人甲状腺乳头状癌组织中成纤维细胞的分离培养方法 |
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US5573937A (en) * | 1989-12-07 | 1996-11-12 | Snow Brand Milk Products Co., Ltd. | Serum free culture medium |
WO2005078070A1 (ja) * | 2004-02-13 | 2005-08-25 | Reprocell, Inc. | 胚性幹細胞用フィーダー細胞作成培地およびフィーダー細胞 |
JP5523674B2 (ja) * | 2005-02-11 | 2014-06-18 | ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト | 植物タンパク質加水分解産物を含有する血清フリーの細胞培養液におけるポリペプチドの生産 |
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- 2010-07-02 EP EP10726999.5A patent/EP2456854B1/fr not_active Not-in-force
- 2010-07-02 NZ NZ597728A patent/NZ597728A/xx not_active IP Right Cessation
- 2010-07-02 CA CA2767929A patent/CA2767929A1/fr not_active Abandoned
- 2010-07-02 MX MX2012001004A patent/MX2012001004A/es active IP Right Grant
- 2010-07-02 JP JP2012520985A patent/JP5758891B2/ja not_active Expired - Fee Related
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- 2010-07-02 WO PCT/EP2010/059478 patent/WO2011009706A1/fr active Application Filing
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Also Published As
Publication number | Publication date |
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EP2456854A1 (fr) | 2012-05-30 |
US20120190109A1 (en) | 2012-07-26 |
AU2010275678A1 (en) | 2012-02-09 |
MX2012001004A (es) | 2012-05-29 |
WO2011009706A1 (fr) | 2011-01-27 |
IL217352A (en) | 2016-03-31 |
IL217352A0 (en) | 2012-02-29 |
FR2948381A1 (fr) | 2011-01-28 |
EP2456854B1 (fr) | 2017-08-30 |
FR2948381B1 (fr) | 2013-07-12 |
JP2013500003A (ja) | 2013-01-07 |
CN102471761A (zh) | 2012-05-23 |
US9243228B2 (en) | 2016-01-26 |
NZ597728A (en) | 2013-11-29 |
ES2650664T3 (es) | 2018-01-19 |
JP5758891B2 (ja) | 2015-08-05 |
AU2010275678B2 (en) | 2016-06-16 |
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