CA2759465C - Method of producing polymers of spider silk proteins - Google Patents

Method of producing polymers of spider silk proteins Download PDF

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Publication number
CA2759465C
CA2759465C CA2759465A CA2759465A CA2759465C CA 2759465 C CA2759465 C CA 2759465C CA 2759465 A CA2759465 A CA 2759465A CA 2759465 A CA2759465 A CA 2759465A CA 2759465 C CA2759465 C CA 2759465C
Authority
CA
Canada
Prior art keywords
amino acid
acid residues
spider silk
liquid medium
silk protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2759465A
Other languages
English (en)
French (fr)
Other versions
CA2759465A1 (en
Inventor
Jan Johansson
My Hedhammar
Anna Rising
Kerstin Nordling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spiber Technologies AB
Original Assignee
Spiber Technologies AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP09158445A external-priority patent/EP2243792A1/en
Application filed by Spiber Technologies AB filed Critical Spiber Technologies AB
Publication of CA2759465A1 publication Critical patent/CA2759465A1/en
Application granted granted Critical
Publication of CA2759465C publication Critical patent/CA2759465C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2759465A 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins Expired - Fee Related CA2759465C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP09158445A EP2243792A1 (en) 2009-04-22 2009-04-22 Methods of producing polymers of spider silk proteins
EP09158445.8 2009-04-22
EP10156923 2010-03-18
EP10156927.5 2010-03-18
EP10156923.4 2010-03-18
EP10156927 2010-03-18
PCT/SE2010/050439 WO2010123450A1 (en) 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins

Publications (2)

Publication Number Publication Date
CA2759465A1 CA2759465A1 (en) 2010-10-28
CA2759465C true CA2759465C (en) 2017-12-12

Family

ID=43011348

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2759465A Expired - Fee Related CA2759465C (en) 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins

Country Status (9)

Country Link
US (1) US8642734B2 (enExample)
EP (1) EP2421890B1 (enExample)
JP (1) JP5912077B2 (enExample)
CN (1) CN102395601A (enExample)
AU (1) AU2010239773B2 (enExample)
CA (1) CA2759465C (enExample)
DK (1) DK2421890T3 (enExample)
RU (1) RU2560437C2 (enExample)
WO (1) WO2010123450A1 (enExample)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102844437B (zh) 2010-03-18 2015-06-03 思百博技术股份公司 蛋白质和多肽的制备
CN103270049B (zh) 2010-10-27 2017-10-13 思百博技术股份公司 用于结合有机靶的蜘蛛丝融合蛋白结构
US9617315B2 (en) 2011-06-01 2017-04-11 Spiber Inc. Artificial polypeptide fiber and method for producing the same
WO2013164405A1 (en) * 2012-05-02 2013-11-07 Spiber Technologies Ab Spider silk fusion protein structures without repetitive fragment for binding to an organic target
EP3973992A3 (en) 2013-09-17 2022-08-31 Bolt Threads, Inc. Methods and compositions for synthesizing improved silk fibers
AU2015358537B2 (en) 2014-12-02 2021-08-19 Evolved By Nature, Inc. Silk performance apparel and products and methods of preparing the same
CN107709571B (zh) * 2015-03-16 2021-11-02 保尔特纺织品公司 改善的丝纤维
CN107735406A (zh) 2015-04-09 2018-02-23 丝芭博株式会社 极性溶剂溶液及其制造方法
EP3281948B1 (en) 2015-04-09 2020-06-10 Spiber Inc. Polar solvent solution and production method thereof
JP2018521239A (ja) 2015-06-11 2018-08-02 ボルト スレッズ インコーポレイテッド 改善された特性を有する組換えタンパク質繊維糸
CN105031723B (zh) * 2015-06-23 2017-05-24 上海交通大学 基于蜘蛛丝蛋白的温敏性水凝胶
BR112018000699B1 (pt) 2015-07-14 2023-10-17 Silk Therapeutics, Inc Artigo e método para revestir um material
JP2018159137A (ja) 2015-08-20 2018-10-11 国立研究開発法人理化学研究所 クモ糸様構造を有するポリペプチド繊維の製造方法
EP3374378B1 (en) 2015-11-13 2021-02-17 Spiber Technologies AB Charge-reversed n-terminal spider silk protein domain and uses thereof
GB201522610D0 (en) * 2015-12-22 2016-02-03 Univ Southampton Protein
RU2018144307A (ru) * 2016-05-16 2020-06-17 Спайбер Текнолоджиз Аб Покрытие из шелка паука для твердых поверхностей
EP3469121A4 (en) * 2016-06-10 2020-01-08 Bolt Threads, Inc. RECOMBINANT PROTEIN FIBER YARNS WITH IMPROVED PROPERTIES
EP3263593A1 (en) 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
CA3035839A1 (en) 2016-09-14 2018-03-22 Bolt Threads, Inc. Long uniform recombinant protein fibers
EP4202394A1 (en) * 2016-11-11 2023-06-28 AMSilk GmbH Use of a shrinkable biopolymer fiber as sensor
CN107475807A (zh) * 2017-08-30 2017-12-15 常州豫春化工有限公司 一种改性尼龙6纤维的制备方法
EP3688017A1 (en) * 2017-09-26 2020-08-05 Spiber Technologies AB Structuring of surface-active macromolecules
EP3688018A4 (en) 2017-09-27 2021-07-07 Evolved by Nature, Inc. SILK COVERED FABRICS, RELATED PRODUCTS AND PREPARATION PROCESSES
JP2021035330A (ja) 2017-09-29 2021-03-04 Spiber株式会社 発現カセット
KR20220029660A (ko) * 2019-07-04 2022-03-08 시비스 머테리얼 사이언스 리미티드 원핵생물 발현 시스템 및 이의 사용 방법
CN110551194B (zh) * 2019-09-19 2023-03-21 天津大学 重组蜘蛛卵鞘丝蛋白复合物及其生成的人造卵鞘丝
AU2021259692A1 (en) 2020-04-23 2022-10-27 Seevix Material Sciences Ltd. Cosmetic compositions comprising dragline spider silk
TW202432573A (zh) * 2022-10-28 2024-08-16 國立臺灣大學 重組蜘蛛絲蛋白及其無標籤省時純化方法
CN218413490U (zh) 2022-10-31 2023-01-31 徐州龙安电子科技有限公司 一种新型蜘蛛电子标签
DE102023105437B3 (de) 2023-03-06 2024-06-13 Dr. Ing. H.C. F. Porsche Aktiengesellschaft Fahrzeugkomponente mit wenigstens einer Fahrzeuginnenraumkomponente

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003201513A1 (en) * 2002-01-11 2003-07-24 Nexia Biotechnologies, Inc. Methods of producing silk polypeptides and products thereof
PL1773875T3 (pl) * 2004-07-22 2015-03-31 Amsilk Gmbh Rekombinowane białka jedwabiu pająków
EP2899204B1 (en) * 2005-12-30 2017-06-28 Spiber Technologies AB Support for cell culture comprising spider silk proteins and methods for producing the same
BRPI0701826B1 (pt) * 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária proteínas da teia de aranha nephilengys cruentata, avicularia juruensis e parawixia bistriata isoladas da biodiversidade brasileira
US9051383B2 (en) * 2007-06-11 2015-06-09 The Regents Of The University Of California Spider silk dragline polynucleotides, polypeptides and methods of use thereof

Also Published As

Publication number Publication date
EP2421890B1 (en) 2015-08-26
AU2010239773B2 (en) 2014-11-06
RU2560437C2 (ru) 2015-08-20
CN102395601A (zh) 2012-03-28
EP2421890A1 (en) 2012-02-29
JP2012531889A (ja) 2012-12-13
RU2011145429A (ru) 2013-05-27
DK2421890T3 (en) 2015-11-23
JP5912077B2 (ja) 2016-04-27
US8642734B2 (en) 2014-02-04
US20120041177A1 (en) 2012-02-16
WO2010123450A1 (en) 2010-10-28
EP2421890A4 (en) 2012-10-24
CA2759465A1 (en) 2010-10-28
AU2010239773A1 (en) 2011-11-03

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CN111094326B (zh) 用于产生丝融合蛋白的浓缩粘合相的方法
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Effective date: 20150420

MKLA Lapsed

Effective date: 20210421