CA2759465C - Method of producing polymers of spider silk proteins - Google Patents

Method of producing polymers of spider silk proteins Download PDF

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Publication number
CA2759465C
CA2759465C CA2759465A CA2759465A CA2759465C CA 2759465 C CA2759465 C CA 2759465C CA 2759465 A CA2759465 A CA 2759465A CA 2759465 A CA2759465 A CA 2759465A CA 2759465 C CA2759465 C CA 2759465C
Authority
CA
Canada
Prior art keywords
amino acid
acid residues
spider silk
liquid medium
silk protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2759465A
Other languages
English (en)
French (fr)
Other versions
CA2759465A1 (en
Inventor
Jan Johansson
My Hedhammar
Anna Rising
Kerstin Nordling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spiber Technologies AB
Original Assignee
Spiber Technologies AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP09158445A external-priority patent/EP2243792A1/en
Application filed by Spiber Technologies AB filed Critical Spiber Technologies AB
Publication of CA2759465A1 publication Critical patent/CA2759465A1/en
Application granted granted Critical
Publication of CA2759465C publication Critical patent/CA2759465C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2759465A 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins Expired - Fee Related CA2759465C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP09158445A EP2243792A1 (en) 2009-04-22 2009-04-22 Methods of producing polymers of spider silk proteins
EP09158445.8 2009-04-22
EP10156923 2010-03-18
EP10156927.5 2010-03-18
EP10156923.4 2010-03-18
EP10156927 2010-03-18
PCT/SE2010/050439 WO2010123450A1 (en) 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins

Publications (2)

Publication Number Publication Date
CA2759465A1 CA2759465A1 (en) 2010-10-28
CA2759465C true CA2759465C (en) 2017-12-12

Family

ID=43011348

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2759465A Expired - Fee Related CA2759465C (en) 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins

Country Status (9)

Country Link
US (1) US8642734B2 (enExample)
EP (1) EP2421890B1 (enExample)
JP (1) JP5912077B2 (enExample)
CN (1) CN102395601A (enExample)
AU (1) AU2010239773B2 (enExample)
CA (1) CA2759465C (enExample)
DK (1) DK2421890T3 (enExample)
RU (1) RU2560437C2 (enExample)
WO (1) WO2010123450A1 (enExample)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5726282B2 (ja) 2010-03-18 2015-05-27 スパイバー テクノロジーズ アーベーSpiber Technologies Ab タンパク質及びポリペプチドの産生
CN103270049B (zh) 2010-10-27 2017-10-13 思百博技术股份公司 用于结合有机靶的蜘蛛丝融合蛋白结构
CN103502516B (zh) * 2011-06-01 2015-04-22 丝芭博株式会社 人造多肽纤维及其制造方法
AU2013255815B2 (en) * 2012-05-02 2018-03-29 Spiber Technologies Ab Spider silk fusion protein structures without repetitive fragment for binding to an organic target
WO2015042164A2 (en) 2013-09-17 2015-03-26 Refactored Materials, Inc. Methods and compositions for synthesizing improved silk fibers
PE20171791A1 (es) 2014-12-02 2017-12-28 Silk Therapeutics Inc Prendas de vestir de seda y productos de alto rendimiento y metodos para elaborarla
CA2979740A1 (en) * 2015-03-16 2016-09-22 Bolt Threads, Inc. Improved silk fibers
EP3281948B1 (en) 2015-04-09 2020-06-10 Spiber Inc. Polar solvent solution and production method thereof
EP3281949A4 (en) 2015-04-09 2018-08-22 Spiber Inc. Polar solvent solution and production method thereof
WO2016201369A1 (en) 2015-06-11 2016-12-15 Bolt Threads, Inc. Recombinant protein fiber yarns with improved properties
CN105031723B (zh) * 2015-06-23 2017-05-24 上海交通大学 基于蜘蛛丝蛋白的温敏性水凝胶
HK1255640A1 (zh) 2015-07-14 2019-08-23 Evolved By Nature, Inc. 丝性能服装和产品及其制备方法
JP2018159137A (ja) 2015-08-20 2018-10-11 国立研究開発法人理化学研究所 クモ糸様構造を有するポリペプチド繊維の製造方法
KR20180070709A (ko) 2015-11-13 2018-06-26 스파이버 테크놀러지스 에이비 전하 역전 n-말단 거미줄 단백질 도메인 및 이의 용도
GB201522610D0 (en) * 2015-12-22 2016-02-03 Univ Southampton Protein
CN109562200B (zh) * 2016-05-16 2022-09-09 思百博技术股份公司 固体表面的蜘蛛丝涂层
JP2019518882A (ja) * 2016-06-10 2019-07-04 ボルト スレッズ インコーポレイテッド 改善された特性を有する組換えタンパク質繊維糸
EP3263593A1 (en) * 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
US11447532B2 (en) 2016-09-14 2022-09-20 Bolt Threads, Inc. Long uniform recombinant protein fibers
WO2018087239A1 (en) * 2016-11-11 2018-05-17 Amsilk Gmbh Use of a shrinkable biopolymer fiber as sensor
CN107475807A (zh) * 2017-08-30 2017-12-15 常州豫春化工有限公司 一种改性尼龙6纤维的制备方法
WO2019063590A1 (en) * 2017-09-26 2019-04-04 Spiber Technologies Ab STRUCTURING SURFACEABLE MACROMOLECULES
CN111712514A (zh) 2017-09-27 2020-09-25 自然进化公司 丝涂布织物和产品及其制备方法
JP2021035330A (ja) 2017-09-29 2021-03-04 Spiber株式会社 発現カセット
AU2020299495A1 (en) * 2019-07-04 2022-01-20 Seevix Material Sciences Ltd. Prokaryotic expression system and methods of using the same
CN110551194B (zh) * 2019-09-19 2023-03-21 天津大学 重组蜘蛛卵鞘丝蛋白复合物及其生成的人造卵鞘丝
JP2023522994A (ja) 2020-04-23 2023-06-01 シービックス マテリアル サイエンシーズ リミテッド 修飾されたスパイダーシルク繊維およびその使用
WO2024091831A2 (en) * 2022-10-28 2024-05-02 National Taiwan University A recombinant spider silk protein and method for tag-free and time-saving purification thereof
CN218413490U (zh) 2022-10-31 2023-01-31 徐州龙安电子科技有限公司 一种新型蜘蛛电子标签
DE102023105437B3 (de) 2023-03-06 2024-06-13 Dr. Ing. H.C. F. Porsche Aktiengesellschaft Fahrzeugkomponente mit wenigstens einer Fahrzeuginnenraumkomponente

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003057727A1 (en) 2002-01-11 2003-07-17 Nexia Biotechnologies, Inc. Methods of producing silk polypeptides and products thereof
EP1773875B1 (en) * 2004-07-22 2014-09-10 Amsilk GmbH Recombinant spider silk proteins
CA2635660C (en) * 2005-12-30 2016-02-23 Spiber Technologies Ab Spider silk proteins and methods for producing spider silk proteins
BRPI0701826B1 (pt) 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária proteínas da teia de aranha nephilengys cruentata, avicularia juruensis e parawixia bistriata isoladas da biodiversidade brasileira
US9051383B2 (en) * 2007-06-11 2015-06-09 The Regents Of The University Of California Spider silk dragline polynucleotides, polypeptides and methods of use thereof

Also Published As

Publication number Publication date
EP2421890A4 (en) 2012-10-24
RU2560437C2 (ru) 2015-08-20
EP2421890B1 (en) 2015-08-26
AU2010239773B2 (en) 2014-11-06
AU2010239773A1 (en) 2011-11-03
US20120041177A1 (en) 2012-02-16
CA2759465A1 (en) 2010-10-28
WO2010123450A1 (en) 2010-10-28
US8642734B2 (en) 2014-02-04
CN102395601A (zh) 2012-03-28
EP2421890A1 (en) 2012-02-29
JP2012531889A (ja) 2012-12-13
RU2011145429A (ru) 2013-05-27
DK2421890T3 (en) 2015-11-23
JP5912077B2 (ja) 2016-04-27

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CN111094326B (zh) 用于产生丝融合蛋白的浓缩粘合相的方法
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EEER Examination request

Effective date: 20150420

MKLA Lapsed

Effective date: 20210421