AU2010239773B2 - Method of producing polymers of spider silk proteins - Google Patents

Method of producing polymers of spider silk proteins Download PDF

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Publication number
AU2010239773B2
AU2010239773B2 AU2010239773A AU2010239773A AU2010239773B2 AU 2010239773 B2 AU2010239773 B2 AU 2010239773B2 AU 2010239773 A AU2010239773 A AU 2010239773A AU 2010239773 A AU2010239773 A AU 2010239773A AU 2010239773 B2 AU2010239773 B2 AU 2010239773B2
Authority
AU
Australia
Prior art keywords
amino acid
spider silk
fragment
silk protein
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2010239773A
Other languages
English (en)
Other versions
AU2010239773A1 (en
Inventor
My Hedhammar
Jan Johansson
Kerstin Nordling
Anna Rising
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spiber Technologies AB
Original Assignee
Spiber Technologies AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP09158445A external-priority patent/EP2243792A1/en
Application filed by Spiber Technologies AB filed Critical Spiber Technologies AB
Publication of AU2010239773A1 publication Critical patent/AU2010239773A1/en
Application granted granted Critical
Publication of AU2010239773B2 publication Critical patent/AU2010239773B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2010239773A 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins Ceased AU2010239773B2 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
EP09158445A EP2243792A1 (en) 2009-04-22 2009-04-22 Methods of producing polymers of spider silk proteins
EP09158445.8 2009-04-22
EP10156923 2010-03-18
EP10156927 2010-03-18
EP10156927.5 2010-03-18
EP10156923.4 2010-03-18
PCT/SE2010/050439 WO2010123450A1 (en) 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins

Publications (2)

Publication Number Publication Date
AU2010239773A1 AU2010239773A1 (en) 2011-11-03
AU2010239773B2 true AU2010239773B2 (en) 2014-11-06

Family

ID=43011348

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2010239773A Ceased AU2010239773B2 (en) 2009-04-22 2010-04-21 Method of producing polymers of spider silk proteins

Country Status (9)

Country Link
US (1) US8642734B2 (enExample)
EP (1) EP2421890B1 (enExample)
JP (1) JP5912077B2 (enExample)
CN (1) CN102395601A (enExample)
AU (1) AU2010239773B2 (enExample)
CA (1) CA2759465C (enExample)
DK (1) DK2421890T3 (enExample)
RU (1) RU2560437C2 (enExample)
WO (1) WO2010123450A1 (enExample)

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EP3168229B1 (en) 2010-03-18 2019-06-19 Spiber Technologies AB Production of proteins and polypeptides
CN103270049B (zh) 2010-10-27 2017-10-13 思百博技术股份公司 用于结合有机靶的蜘蛛丝融合蛋白结构
JP5540154B2 (ja) * 2011-06-01 2014-07-02 スパイバー株式会社 人造ポリペプチド繊維
CN104394953B (zh) * 2012-05-02 2018-01-23 思百博技术股份公司 用于结合有机靶的没有重复片段的蜘蛛丝融合蛋白结构
CA2924343C (en) 2013-09-17 2025-09-16 Bolt Threads, Inc. PROCESSES AND COMPOSITIONS FOR SYNTHESIZING IMPROVED SILK FIBERS
SG11201704494SA (en) 2014-12-02 2017-06-29 Silk Therapeutics Inc Silk performance apparel and products and methods of preparing the same
CN107709571B (zh) * 2015-03-16 2021-11-02 保尔特纺织品公司 改善的丝纤维
JP6856828B2 (ja) 2015-04-09 2021-04-14 Ｓｐｉｂｅｒ株式会社 極性溶媒溶液及びその製造方法
CN107849100B (zh) 2015-04-09 2022-02-08 丝芭博株式会社 极性溶剂溶液及其制造方法
US20180216260A1 (en) 2015-06-11 2018-08-02 Bolt Threads, Inc. Recombinant protein fiber yarns with improved properties
CN105031723B (zh) * 2015-06-23 2017-05-24 上海交通大学 基于蜘蛛丝蛋白的温敏性水凝胶
CN108135975A (zh) 2015-07-14 2018-06-08 丝绸医疗公司 丝性能服装和产品及其制备方法
JP2018159137A (ja) 2015-08-20 2018-10-11 国立研究開発法人理化学研究所 クモ糸様構造を有するポリペプチド繊維の製造方法
KR20180070709A (ko) 2015-11-13 2018-06-26 스파이버 테크놀러지스 에이비 전하 역전 n-말단 거미줄 단백질 도메인 및 이의 용도
GB201522610D0 (en) * 2015-12-22 2016-02-03 Univ Southampton Protein
WO2017198655A1 (en) * 2016-05-16 2017-11-23 Spiber Technologies Ab Spider silk coating of solid surfaces
US20200032424A1 (en) * 2016-06-10 2020-01-30 Bolt Threads, Inc. Recombinant protein fiber yarns with improved properties
EP3263593A1 (en) * 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
US11447532B2 (en) 2016-09-14 2022-09-20 Bolt Threads, Inc. Long uniform recombinant protein fibers
WO2018087239A1 (en) * 2016-11-11 2018-05-17 Amsilk Gmbh Use of a shrinkable biopolymer fiber as sensor
CN107475807A (zh) * 2017-08-30 2017-12-15 常州豫春化工有限公司 一种改性尼龙6纤维的制备方法
EP3688017A1 (en) * 2017-09-26 2020-08-05 Spiber Technologies AB Structuring of surface-active macromolecules
US11390988B2 (en) 2017-09-27 2022-07-19 Evolved By Nature, Inc. Silk coated fabrics and products and methods of preparing the same
JP2021035330A (ja) * 2017-09-29 2021-03-04 Ｓｐｉｂｅｒ株式会社 発現カセット
WO2020162627A1 (ja) * 2019-02-07 2020-08-13 Ｓｐｉｂｅｒ株式会社 人造構造タンパク質繊維の製造方法
US12668828B2 (en) 2019-07-04 2026-06-30 Seevix Material Sciences Ltd. Prokaryotic expression system and methods of using the same
CN110551194B (zh) * 2019-09-19 2023-03-21 天津大学 重组蜘蛛卵鞘丝蛋白复合物及其生成的人造卵鞘丝
KR20230005173A (ko) 2020-04-23 2023-01-09 시비스 머테리얼 사이언스 리미티드 드래그라인 스파이더 실크를 포함하는 화장료 조성물
KR20230112616A (ko) * 2020-10-13 2023-07-27 아비타이드 엘엘씨 3중-나선 번들 단백질들의 친화성 리간드 라이브러리 및 이의 용도
WO2022218499A1 (en) * 2021-04-12 2022-10-20 Alphabeta Ab Facilitated passage over the blood-brain barrier by co-administration of bri2 brichos domain and lipid microbubbles and/or nanodroplets
WO2024091831A2 (en) * 2022-10-28 2024-05-02 National Taiwan University A recombinant spider silk protein and method for tag-free and time-saving purification thereof
CN218413490U (zh) 2022-10-31 2023-01-31 徐州龙安电子科技有限公司 一种新型蜘蛛电子标签
DE102023105437B3 (de) 2023-03-06 2024-06-13 Dr. Ing. H.C. F. Porsche Aktiengesellschaft Fahrzeugkomponente mit wenigstens einer Fahrzeuginnenraumkomponente

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008154547A2 (en) * 2007-06-11 2008-12-18 The Regents Of The University Of California Spider silk dragline polynucleotides, polypeptides and methods of use thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003057727A1 (en) * 2002-01-11 2003-07-17 Nexia Biotechnologies, Inc. Methods of producing silk polypeptides and products thereof
AU2005263622B2 (en) * 2004-07-22 2012-04-26 Amsilk Gmbh Recombinant spider silk proteins
RU2444527C2 (ru) * 2005-12-30 2012-03-10 Спибер Текнолоджиз Аб Рекомбинантный спидроиновый белок главной ампулярной железы, способный к полимеризации, рекомбинантный слитый белок, способный к управляемой полимеризации, полимер спидроинового белка главной ампулярной железы, композиция для управляемой полимеризации, рекомбинантная молекула нуклеиновой кислоты (варианты), способ получения растворимого слитого белка, способ получения полимера спидроинового белка главной ампулярной железы и способ культивирования эукариотических клеток
BRPI0701826B1 (pt) 2007-03-16 2021-02-17 Embrapa - Empresa Brasileira De Pesquisa Agropecuária proteínas da teia de aranha nephilengys cruentata, avicularia juruensis e parawixia bistriata isoladas da biodiversidade brasileira

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008154547A2 (en) * 2007-06-11 2008-12-18 The Regents Of The University Of California Spider silk dragline polynucleotides, polypeptides and methods of use thereof

Also Published As

Publication number Publication date
US20120041177A1 (en) 2012-02-16
CA2759465A1 (en) 2010-10-28
WO2010123450A1 (en) 2010-10-28
RU2560437C2 (ru) 2015-08-20
EP2421890B1 (en) 2015-08-26
JP2012531889A (ja) 2012-12-13
EP2421890A1 (en) 2012-02-29
CN102395601A (zh) 2012-03-28
AU2010239773A1 (en) 2011-11-03
DK2421890T3 (en) 2015-11-23
EP2421890A4 (en) 2012-10-24
CA2759465C (en) 2017-12-12
US8642734B2 (en) 2014-02-04
RU2011145429A (ru) 2013-05-27
JP5912077B2 (ja) 2016-04-27

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Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired