CA2744804C - Synaptic vesicle cycling assays and systems - Google Patents

Synaptic vesicle cycling assays and systems Download PDF

Info

Publication number
CA2744804C
CA2744804C CA2744804A CA2744804A CA2744804C CA 2744804 C CA2744804 C CA 2744804C CA 2744804 A CA2744804 A CA 2744804A CA 2744804 A CA2744804 A CA 2744804A CA 2744804 C CA2744804 C CA 2744804C
Authority
CA
Canada
Prior art keywords
synaptic vesicle
electrode
wells
platform
vesicle cycling
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2744804A
Other languages
English (en)
French (fr)
Other versions
CA2744804A1 (en
Inventor
David J. Gerber
Jeffrey R. Cottrell
Timothy A. Ryan
Jonathan M. Levenson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Galenea Corp
Original Assignee
Galenea Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Galenea Corp filed Critical Galenea Corp
Publication of CA2744804A1 publication Critical patent/CA2744804A1/en
Application granted granted Critical
Publication of CA2744804C publication Critical patent/CA2744804C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058Neurological cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value
    • G01N2500/10Screening for compounds of potential therapeutic value involving cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Biophysics (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
CA2744804A 2008-09-04 2009-08-31 Synaptic vesicle cycling assays and systems Expired - Fee Related CA2744804C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9436108P 2008-09-04 2008-09-04
US61/094,361 2008-09-04
PCT/US2009/004932 WO2010027446A2 (en) 2008-09-04 2009-08-31 Synaptic vesicle cycling assays and systems

Publications (2)

Publication Number Publication Date
CA2744804A1 CA2744804A1 (en) 2010-03-11
CA2744804C true CA2744804C (en) 2019-02-05

Family

ID=41479300

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2744804A Expired - Fee Related CA2744804C (en) 2008-09-04 2009-08-31 Synaptic vesicle cycling assays and systems

Country Status (8)

Country Link
US (2) US20120053084A1 (https=)
EP (1) EP2329275A2 (https=)
JP (2) JP5789513B2 (https=)
KR (1) KR20110073494A (https=)
CN (1) CN102203622A (https=)
AU (2) AU2009288657A1 (https=)
CA (1) CA2744804C (https=)
WO (1) WO2010027446A2 (https=)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009100082A1 (en) * 2008-02-04 2009-08-13 Bayer Healthcare Llc Semiconductor based analyte sensors and methods
US9217155B2 (en) 2008-05-28 2015-12-22 University Of Massachusetts Isolation of novel AAV'S and uses thereof
US8734809B2 (en) 2009-05-28 2014-05-27 University Of Massachusetts AAV's and uses thereof
US9207237B2 (en) 2010-08-23 2015-12-08 President And Fellows Of Harvard College Systems, methods, and workflows for optogenetics analysis
CA2838330C (en) 2010-08-23 2021-01-26 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential
US9488637B2 (en) * 2011-01-26 2016-11-08 The Curators Of The University Of Missouri Combination of single-cell electroporation and electrical recording using the same electrode
EP3318635A1 (en) 2011-04-21 2018-05-09 University of Massachusetts Raav-based compositions and methods for treating alpha-1 anti-trypsin deficiencies
EP2798391A2 (en) * 2011-11-23 2014-11-05 President and Fellows of Harvard College Systems and methods for imaging at high spatial and/or temporal precision
US9696275B2 (en) 2012-10-12 2017-07-04 The Curators Of The University Of Missouri Addressable electrode arrays in multiple fluidic compartments and uses thereof
WO2014065329A1 (ja) * 2012-10-25 2014-05-01 浜松ホトニクス株式会社 細胞観察装置及び細胞観察方法
WO2014065330A1 (ja) 2012-10-25 2014-05-01 浜松ホトニクス株式会社 細胞観察装置、電気刺激装置、及び細胞観察方法
JP6370544B2 (ja) * 2013-11-11 2018-08-08 浜松ホトニクス株式会社 細胞観察装置及び細胞観察方法
WO2015107761A1 (ja) * 2014-01-16 2015-07-23 浜松ホトニクス株式会社 細胞観察装置、電気刺激装置、及び細胞観察方法
US10072251B2 (en) 2014-02-19 2018-09-11 University Of Massachusetts Recombinant AAVS having useful transcytosis properties
CA2942515C (en) 2014-03-18 2025-12-09 University Of Massachusetts Raav-based compositions and methods for treating amyotrophic lateral sclerosis
EP3134729A1 (en) 2014-04-22 2017-03-01 Q-State Biosciences, Inc. Analysis of compounds for pain and sensory disorders
WO2015164786A1 (en) 2014-04-25 2015-10-29 University Of Massachusetts Recombinant aav vectors useful for reducing immunity against transgene products
WO2015195769A2 (en) 2014-06-18 2015-12-23 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential
US10625234B2 (en) 2014-08-28 2020-04-21 StemoniX Inc. Method of fabricating cell arrays and uses thereof
US10370432B2 (en) 2014-10-03 2019-08-06 University Of Massachusetts Heterologous targeting peptide grafted AAVS
EP3200830B1 (en) 2014-10-03 2020-09-09 University of Massachusetts High efficiency library-identified aav vectors
CN107073051B (zh) 2014-10-21 2021-08-24 马萨诸塞大学 重组aav变体及其用途
WO2016115333A1 (en) 2015-01-15 2016-07-21 President And Fellows Of Harvard College Optical selection of cells
EP3256170B1 (en) 2015-02-13 2020-09-23 University of Massachusetts Compositions and methods for transient delivery of nucleases
US10048275B2 (en) 2015-03-13 2018-08-14 Q-State Biosciences, Inc. Cardiotoxicity screening methods
EP3285788B1 (en) 2015-04-23 2024-12-18 University of Massachusetts Modulation of aav vector transgene expression
US11046955B2 (en) 2015-04-24 2021-06-29 University Of Massachusetts Modified AAV constructs and uses thereof
US11248212B2 (en) 2015-06-30 2022-02-15 StemoniX Inc. Surface energy directed cell self assembly
US10760053B2 (en) 2015-10-15 2020-09-01 StemoniX Inc. Method of manufacturing or differentiating mammalian pluripotent stem cells or progenitor cells using a hollow fiber bioreactor
EP3364997B1 (en) 2015-10-22 2024-01-17 University of Massachusetts Aspartoacylase gene therapy in the treatment of canavan disease
WO2017070516A1 (en) 2015-10-22 2017-04-27 University Of Massachusetts Prostate-targeting adeno-associated virus serotype vectors
CA3011939A1 (en) 2016-02-02 2017-08-10 University Of Massachusetts Method to enhance the efficiency of systemic aav gene delivery to the central nervous system
CA3012344A1 (en) 2016-02-12 2017-08-17 University Of Massachusetts Anti-angiogenic mirna therapeutics for inhibiting corneal neovascularization
EP3225279B1 (en) * 2016-03-31 2023-12-20 Université de Rennes Brain tissue stimulation apparatus and computer program
US11207426B2 (en) 2016-04-05 2021-12-28 University Of Massachusetts Compositions and methods for selective inhibition of grainyhead-like protein expression
US11413356B2 (en) 2016-04-15 2022-08-16 University Of Massachusetts Methods and compositions for treating metabolic imbalance
US11882815B2 (en) 2016-06-15 2024-01-30 University Of Massachusetts Recombinant adeno-associated viruses for delivering gene editing molecules to embryonic cells
US10457940B2 (en) 2016-09-22 2019-10-29 University Of Massachusetts AAV treatment of Huntington's disease
AU2017341849B2 (en) 2016-10-13 2024-03-21 University Of Massachusetts AAV capsid designs
US11859179B2 (en) 2017-05-09 2024-01-02 University Of Massachusetts Methods of treating amyotrophic lateral sclerosis (ALS)
CN111448321A (zh) 2017-09-22 2020-07-24 马萨诸塞大学 Sod1双表达载体及其用途
CN112203697A (zh) 2018-04-13 2021-01-08 马萨诸塞大学 编码氨基己糖苷酶alpha和beta亚基的双顺反子AAV载体及其用途
JP7065433B2 (ja) * 2018-08-14 2022-05-12 日本電信電話株式会社 電極及びその製造方法、並びに積層体
US12448629B2 (en) 2018-10-05 2025-10-21 University Of Massachusetts rAAV vectors for the treatment of GM1 and GM2 gangliosidosis
IL325928A (en) * 2019-07-16 2026-03-01 Meso Scale Technologies Llc Testing instruments, methods and reagents
EP4271982A4 (en) * 2020-12-31 2024-11-27 Q-State Biosciences, Inc. Optical plate controller and reader
EP4271978B1 (en) 2020-12-31 2026-05-06 Quiver Holdings Inc. Microscope with spatial imaging and beam homogenizer
JP2024501848A (ja) 2020-12-31 2024-01-16 キュー-ステート バイオサイエンシーズ, インコーポレイテッド 光遺伝学のためのイメージャの較正および正規化
EP4271789A4 (en) * 2020-12-31 2024-11-27 Q-State Biosciences, Inc. PLATE IMAGE PRODUCER
US20220357313A1 (en) * 2021-05-04 2022-11-10 Q-State Biosciences, Inc. Drug fingerprinting
EP4363832A4 (en) 2021-06-30 2025-05-07 Q-State Biosciences, Inc. MULTIPLEX OPTICAL STIMULUS AND READOUT PROCESS
WO2023210585A1 (ja) * 2022-04-25 2023-11-02 株式会社Jiksak Bioengineering 標的化剤
WO2025084395A1 (ja) * 2023-10-19 2025-04-24 株式会社Jiksak Bioengineering 抗シナプトタグミン2抗体
WO2025089346A1 (ja) * 2023-10-25 2025-05-01 株式会社Jiksak Bioengineering 抗シナプトフィジン抗体

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000004366A1 (en) * 1998-07-17 2000-01-27 Aurora Biosciences Corporation Detector and screening device for ion channels
US7399599B2 (en) * 2000-07-10 2008-07-15 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
US7615356B2 (en) * 2000-07-10 2009-11-10 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
EP1425065A4 (en) * 2001-07-12 2004-12-15 Merck & Co Inc ELECTRIC FIELD STIMULATION WITH EUKARYONTIC CELLS
US7221981B2 (en) * 2002-03-28 2007-05-22 Northstar Neuroscience, Inc. Electrode geometries for efficient neural stimulation
US20070274923A1 (en) * 2005-01-11 2007-11-29 Philippe Brulet Non-invasive real-time in vivo bioluminescence imaging of local Ca2+ dynamics in living organisms

Also Published As

Publication number Publication date
WO2010027446A4 (en) 2010-08-05
WO2010027446A3 (en) 2010-05-20
JP2016019736A (ja) 2016-02-04
JP6106225B2 (ja) 2017-03-29
US20180136198A1 (en) 2018-05-17
WO2010027446A2 (en) 2010-03-11
AU2016202387A1 (en) 2016-05-05
CA2744804A1 (en) 2010-03-11
EP2329275A2 (en) 2011-06-08
KR20110073494A (ko) 2011-06-29
JP2012501646A (ja) 2012-01-26
US20120053084A1 (en) 2012-03-01
AU2009288657A1 (en) 2010-03-11
JP5789513B2 (ja) 2015-10-07
CN102203622A (zh) 2011-09-28

Similar Documents

Publication Publication Date Title
CA2744804C (en) Synaptic vesicle cycling assays and systems
Liu et al. Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy
Dong et al. Unlocking opioid neuropeptide dynamics with genetically encoded biosensors
Koizumi et al. Structural-functional properties of identified excitatory and inhibitory interneurons within pre-Bötzinger complex respiratory microcircuits
Lalo et al. Exocytosis of ATP from astrocytes modulates phasic and tonic inhibition in the neocortex
Jouhanneau et al. Cortical fosGFP expression reveals broad receptive field excitatory neurons targeted by POm
Szabo et al. Ripple-selective GABAergic projection cells in the hippocampus
Park et al. Function and circuitry of VIP+ interneurons in the mouse retina
Gore et al. Visualization of plasticity in fear-evoked calcium signals in midbrain dopamine neurons
Hisano Vesicular glutamate transporters in the brain
JP5953293B2 (ja) 発光タンパクによる長期モニタリング方法および解析方法
Hacker et al. Evidence for two populations of bitter responsive taste cells in mice
JP6462684B2 (ja) カルシウム指示遺伝子
US20060063202A1 (en) High throughput method and system for screening candidate compounds for activity against epilepsy and other neurological diseases
Shannonhouse et al. Lessons from the use of in vivo cellular calcium imaging in primary sensory neurons and spinal cord
DE10108263A1 (de) Analyse von Modifizierungen und Demodifizierungen von Proteinen mit Ubiquitinverwandten Proteinen mittels FRET (Fluorescence Resonance Energy Transfer)
EP3042194B1 (en) Human cellular models with biosensors
US20210161383A1 (en) Chromatic bioluminescence as a cellular level readout system of neural activity
Tian et al. Unlocking opioid neuropeptide dynamics with genetically-encoded biosensors
WO2000020859A1 (en) METHOD AND APPARATUS FOR DETECTING BINDING INTERACTIONS $i(IN VIVO)
MX2012003773A (es) Genes. metodos y composiciones relacionadas con la neurogenesis y su modulacion.
HK1162671A (en) Synaptic vesicle cycling assays and systems
WO2003093442A2 (en) Cell cultures from animal models of alzheimer's disease for screening and testing drug efficacy
Gu Investigation of Exocytosis for a New Paradigm of Plasticity in Biological Systems Using Electrochemistry and Mass Spectrometry Imaging
Liu Multimodal High-Content Optimization of Neural Activity Biosensors

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20140827

MKLA Lapsed

Effective date: 20220301

MKLA Lapsed

Effective date: 20200831