CA2738024A1 - Methods for purification of viruses - Google Patents
Methods for purification of viruses Download PDFInfo
- Publication number
- CA2738024A1 CA2738024A1 CA2738024A CA2738024A CA2738024A1 CA 2738024 A1 CA2738024 A1 CA 2738024A1 CA 2738024 A CA2738024 A CA 2738024A CA 2738024 A CA2738024 A CA 2738024A CA 2738024 A1 CA2738024 A1 CA 2738024A1
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- Prior art keywords
- virus
- cells
- cell
- viruses
- specific embodiment
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Classifications
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- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- A61K2039/525—Virus
- A61K2039/5254—Virus avirulent or attenuated
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16051—Methods of production or purification of viral material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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| CN103361319A (zh) | 2006-09-15 | 2013-10-23 | 米迪缪尼有限公司 | 支持病毒生长到高效价的mdck细胞系和采用该细胞系的生物反应器方法 |
| KR20110074563A (ko) | 2008-09-24 | 2011-06-30 | 메디뮨 엘엘씨 | 바이러스의 정제 방법 |
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| WO2011151723A2 (en) * | 2010-06-01 | 2011-12-08 | Novartis Ag | Concentration of vaccine antigens without lyophilization |
| CN108404453B (zh) | 2010-07-30 | 2021-01-05 | Emd密理博公司 | 层析介质和方法 |
| EP2625263B1 (en) | 2010-10-08 | 2020-03-11 | Terumo BCT, Inc. | Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system |
| US9611458B2 (en) * | 2010-12-27 | 2017-04-04 | Kyowa Hakko Kirin Co., Ltd | Method for preparing aqueous solution containing culture medium and chelating agent |
| CN102199198A (zh) * | 2011-03-28 | 2011-09-28 | 北京交通大学 | 人呼吸道合胞病毒f蛋白的纯化方法 |
| ES2653197T3 (es) * | 2011-07-20 | 2018-02-06 | Bgp Products B.V. | Procedimiento para producir antígeno ortomixoviral y vacunas |
| US10633625B2 (en) | 2013-11-16 | 2020-04-28 | Terumo Bct, Inc. | Expanding cells in a bioreactor |
| CN106232800B (zh) | 2014-03-25 | 2020-07-03 | 泰尔茂比司特公司 | 介质的被动替代 |
| WO2015175679A2 (en) * | 2014-05-13 | 2015-11-19 | Amgen Inc. | Process control systems and methods for use with filters and filtration processes |
| US10449517B2 (en) | 2014-09-02 | 2019-10-22 | Emd Millipore Corporation | High surface area fiber media with nano-fibrillated surface features |
| WO2016049421A1 (en) | 2014-09-26 | 2016-03-31 | Terumo Bct, Inc. | Scheduled feed |
| CA2966515C (en) | 2014-12-08 | 2021-04-27 | Emd Millipore Corporation | Mixed bed ion exchange adsorber |
| WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
| WO2017053239A1 (en) * | 2015-09-22 | 2017-03-30 | Sanofi Pasteur Biologics, Llc | Purification of respiratory syncytial virus |
| CN106337045A (zh) * | 2016-05-24 | 2017-01-18 | 微基生物科技（上海）有限公司 | 一种防止pcr污染的方法及其应用 |
| WO2017205667A1 (en) | 2016-05-25 | 2017-11-30 | Terumo Bct, Inc. | Cell expansion |
| US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
| US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
| WO2018183426A1 (en) * | 2017-03-30 | 2018-10-04 | Merck Sharp & Dohme Corp. | Addition of nucleases directly to cell culture to facilitate digestion and clearance of host cell nucleic acids |
| US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
| US12234441B2 (en) | 2017-03-31 | 2025-02-25 | Terumo Bct, Inc. | Cell expansion |
| JP7393945B2 (ja) | 2017-03-31 | 2023-12-07 | テルモ ビーシーティー、インコーポレーテッド | 細胞増殖 |
| CN111655844B (zh) * | 2017-10-16 | 2024-05-24 | 印度血清研究所私人有限公司 | 包含特别是减毒活重组黄病毒的稳定的疫苗组合物及其制备方法 |
| US11339377B2 (en) * | 2017-11-08 | 2022-05-24 | Bluesky Immunotherapies Gmbh | SO3 chromatography for use in a method for virus purification |
| JP7042341B2 (ja) | 2017-12-07 | 2022-03-25 | メルク・シャープ・アンド・ドーム・コーポレーション | デングウイルスワクチン組成物の製剤 |
| US10988726B2 (en) | 2018-08-19 | 2021-04-27 | Cytera Cellworks Limited | Systems and methods for automated cell culturing |
| DE102018215551A1 (de) * | 2018-09-12 | 2020-03-12 | Virolutions Biotech Gmbh | Verfahren zur Herstellung eines antitumoralen Arenavirus sowie Arenavirusmutanten |
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| CN111763661B (zh) * | 2019-04-02 | 2022-07-29 | 普莱柯生物工程股份有限公司 | 一种病毒纯化的方法、制备的疫苗组合物及其应用 |
| AU2021233167A1 (en) * | 2020-03-12 | 2022-09-22 | Bavarian Nordic A/S | Compositions improving poxvirus stability |
| CA3194520A1 (en) * | 2020-06-11 | 2021-12-16 | 2Seventy Bio, Inc. | Methods for manufacturing viral vectors |
| TW202208399A (zh) * | 2020-06-17 | 2022-03-01 | 美商梅薩疫苗公司 | 嵌合呼吸道合胞病毒(rsv)及冠狀病毒蛋白、免疫源性組合物及使用方法 |
| JP7705950B2 (ja) | 2021-03-23 | 2025-07-10 | テルモ ビーシーティー、インコーポレーテッド | 細胞を増殖する方法 |
| JP7716233B2 (ja) * | 2021-05-28 | 2025-07-31 | ダイセン・メンブレン・システムズ株式会社 | 微小有用物質の分離精製方法と分離精製装置 |
| CN113930353B (zh) * | 2021-08-31 | 2023-07-07 | 广西大学 | 一种耐亚硒酸盐的粘质沙雷氏菌及其还原特性鉴定方法 |
| JP7764758B2 (ja) | 2021-12-27 | 2025-11-06 | 株式会社島津製作所 | 濃縮装置、それを備えた分析システムおよび濃縮方法 |
| US12209689B2 (en) | 2022-02-28 | 2025-01-28 | Terumo Kabushiki Kaisha | Multiple-tube pinch valve assembly |
| USD1099116S1 (en) | 2022-09-01 | 2025-10-21 | Terumo Bct, Inc. | Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device |
| CN115505571B (zh) * | 2022-11-24 | 2023-03-10 | 上海倍谙基生物科技有限公司 | 适用于悬浮培养lmh细胞生产禽腺病毒的无血清添加剂 |
Family Cites Families (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO162160C (no) | 1987-01-09 | 1989-11-15 | Medi Cult As | Serumfritt vekstmedium, samt anvendelse derav. |
| US5118513A (en) | 1987-07-02 | 1992-06-02 | The Procter & Gamble Company | Method for enhancing bioavailability of iron-calcium mineral supplements |
| US20060019350A1 (en) | 1989-08-28 | 2006-01-26 | Peter Palese | Recombinant negative strand RNA virus expression system and vacccines |
| US5840520A (en) | 1989-08-28 | 1998-11-24 | Aviron | Recombinant negative strand RNA virus expression systems |
| US5854037A (en) | 1989-08-28 | 1998-12-29 | The Mount Sinai School Of Medicine Of The City University Of New York | Recombinant negative strand RNA virus expression systems and vaccines |
| US5786199A (en) | 1989-08-28 | 1998-07-28 | The Mount Sinai School Of Medicine Of The City University Of New York | Recombinant negative strand RNA virus expression systems and vaccines |
| US5166057A (en) | 1989-08-28 | 1992-11-24 | The Mount Sinai School Of Medicine Of The City University Of New York | Recombinant negative strand rna virus expression-systems |
| US6001634A (en) | 1989-08-28 | 1999-12-14 | Palese; Peter | Recombinant negative strand RNA viruses |
| US6887699B1 (en) | 1990-05-22 | 2005-05-03 | Medimmune Vaccines, Inc. | Recombinant negative strand RNA virus expression systems and vaccines |
| US6245549B1 (en) | 1990-06-28 | 2001-06-12 | Connaught Laboratories Limited | Production of virus and purification of viral envelope proteins for vaccine use |
| GB9215834D0 (en) | 1992-07-24 | 1992-09-09 | Celltech Ltd | Cell culture |
| US5824536A (en) | 1994-08-23 | 1998-10-20 | St. Jude Children's Research Hospital | Influenza virus replicated in mammalian cell culture and vaccine production |
| US7465574B2 (en) | 1994-09-30 | 2008-12-16 | Medimmune, Llc | Recombinant RSV virus expression systems and vaccines |
| DE19612966B4 (de) | 1996-04-01 | 2009-12-10 | Novartis Vaccines And Diagnostics Gmbh & Co. Kg | MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren |
| KR100503701B1 (ko) | 1996-11-20 | 2005-07-26 | 인트로겐 테라페티스, 인코퍼레이티드 | 아데노바이러스 벡터를 생산하고 정제하는 개선된 방법 |
| US6544785B1 (en) | 1998-09-14 | 2003-04-08 | Mount Sinai School Of Medicine Of New York University | Helper-free rescue of recombinant negative strand RNA viruses |
| US8715940B2 (en) | 1999-04-06 | 2014-05-06 | Wisconsin Alumni Research Foundation | Method of making recombinant influenza virus |
| ATE384120T1 (de) | 1999-08-27 | 2008-02-15 | Invitrogen Corp | Metallbindende verbindungen und deren verwendung in zusammensetzungen für zellkulturmedien |
| US6544424B1 (en) | 1999-12-03 | 2003-04-08 | Refined Technology Company | Fluid filtration system |
| SI1317559T1 (sl) | 2000-04-28 | 2009-04-30 | St Jude Childrens Res Hospital | Sistem transfekcije dna za pripravo infektivne negativne verige virusne rna |
| DE60139319D1 (en) * | 2000-06-23 | 2009-09-03 | Intervet Int Bv | Us |
| DE10144903A1 (de) | 2001-09-12 | 2003-03-27 | Chiron Behring Gmbh & Co | Vermehrung von Viren in Zellkultur |
| DE10144906B4 (de) | 2001-09-12 | 2013-11-28 | Novartis Vaccines And Diagnostics Gmbh | Verfahren zur großtechnischen Herstellung von Impfstoffen |
| US6951752B2 (en) | 2001-12-10 | 2005-10-04 | Bexter Healthcare S.A. | Method for large scale production of virus antigen |
| EP1485488A4 (en) | 2002-02-21 | 2005-03-02 | Sinai School Medicine | RECOMBINANT EXPRESSION SYSTEMS FOR MINUSTRAN VIRUS RNA AND VACCINES |
| WO2003086443A1 (en) | 2002-04-11 | 2003-10-23 | Medimmune Vaccines, Inc. | Spray freeze dry of compositions for intranasal administration |
| US7465456B2 (en) | 2002-04-26 | 2008-12-16 | Medimmune, Llc | Multi plasmid system for the production of influenza virus |
| ES2526191T3 (es) | 2002-04-26 | 2015-01-08 | Medimmune, Llc | Método para producir virus influenza B infeccioso en cultivo celular |
| MXPA04010603A (es) | 2002-04-30 | 2004-12-13 | Oncolytics Biotech Inc | Metodos mejorados de purificacion viral. |
| US6825306B2 (en) | 2002-08-16 | 2004-11-30 | The Goodyear Tire & Rubber Company | Functionalized monomers for synthesis of rubbery polymers |
| JP2004135665A (ja) | 2002-09-25 | 2004-05-13 | Nihon Pharmaceutical Co Ltd | ウイルスの分離法 |
| US20060002862A1 (en) * | 2002-12-17 | 2006-01-05 | Medimmune Vaccines, Inc. | High pressure spray-dry of bioactive materials |
| KR101190932B1 (ko) | 2003-02-25 | 2012-10-16 | 메디뮨 엘엘씨 | 인플루엔자 백신 조성물의 제조 방법 |
| US7687240B2 (en) * | 2003-03-18 | 2010-03-30 | Wyeth Holdings Corporation | Process for increasing RSV surface glycoprotein yields using a mutant strain of RSV |
| EP2494986A1 (en) | 2003-04-25 | 2012-09-05 | MedImmune Vaccines, Inc. | Metapneumovirus strains and their use in vaccine formulations and as vectors for expression of antigenic sequences and methods for propagating virus |
| JP2006525815A (ja) | 2003-05-28 | 2006-11-16 | ウィスコンシン・アラムナイ・リサーチ・ファウンデイション | Ｐｏｌｉｉプロモーターおよびリボザイムを有する組換えインフルエンザベクター |
| JP4771959B2 (ja) | 2003-12-23 | 2011-09-14 | メディミューン，エルエルシー | インフルエンザウイルス作製のための多重プラスミド系 |
| ES2329607T3 (es) * | 2004-02-23 | 2009-11-27 | Crucell Holland B.V. | Metodos de purificacion de virus. |
| AU2005245943A1 (en) | 2004-05-20 | 2005-12-01 | Id Biomedical Corporation | Process for the production of an influenza vaccine |
| CN102302773B (zh) | 2004-10-06 | 2014-05-14 | 米迪缪尼有限公司 | 冷藏温度稳定的流感疫苗组合物 |
| EP2368975B1 (en) * | 2004-12-23 | 2014-09-17 | MedImmune, LLC | Non-tumorigenic MDCK cell line for propagating viruses |
| US7790434B2 (en) | 2005-06-21 | 2010-09-07 | Medimmune, Llc | Methods and compositions for expressing negative-sense viral RNA in canine cells |
| EP1917361A4 (en) | 2005-06-21 | 2009-07-29 | Medimmune Vaccines Inc | METHODS AND COMPOSITIONS FOR EXPRESSING NEGATIVE SENSE RNA VIRUS IN CANINE CELLS |
| AU2007240448B8 (en) | 2006-04-19 | 2014-03-06 | Medimmune Llc. | Methods and compositions for expressing negative-sense viral RNA in canine cells |
| DK2007883T3 (da) * | 2006-04-20 | 2012-02-06 | Wyeth Llc | Rensningsfremgangsmåder til isolering af renset vesikulær stomatitis-virus fra cellekultur |
| CN103361319A (zh) | 2006-09-15 | 2013-10-23 | 米迪缪尼有限公司 | 支持病毒生长到高效价的mdck细胞系和采用该细胞系的生物反应器方法 |
| US8202726B2 (en) | 2008-09-24 | 2012-06-19 | Medimmune, Llc | Methods for cultivating cells, propagating and purifying viruses |
| KR20110074563A (ko) | 2008-09-24 | 2011-06-30 | 메디뮨 엘엘씨 | 바이러스의 정제 방법 |
-
2009
- 2009-09-24 KR KR1020117009305A patent/KR20110074563A/ko not_active Abandoned
- 2009-09-24 WO PCT/US2009/058174 patent/WO2010036774A1/en not_active Ceased
- 2009-09-24 AU AU2009296628A patent/AU2009296628B2/en not_active Ceased
- 2009-09-24 CN CN2009801468459A patent/CN102215865B/zh not_active Expired - Fee Related
- 2009-09-24 BR BRPI0919250A patent/BRPI0919250A2/pt not_active IP Right Cessation
- 2009-09-24 JP JP2011529214A patent/JP5548207B2/ja active Active
- 2009-09-24 CA CA2738024A patent/CA2738024A1/en not_active Abandoned
- 2009-09-24 US US13/120,638 patent/US8785173B2/en active Active
- 2009-09-24 EP EP09816835A patent/EP2334328A4/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| AU2009296628B2 (en) | 2015-01-15 |
| US8785173B2 (en) | 2014-07-22 |
| US20120219588A1 (en) | 2012-08-30 |
| EP2334328A1 (en) | 2011-06-22 |
| JP5548207B2 (ja) | 2014-07-16 |
| KR20110074563A (ko) | 2011-06-30 |
| BRPI0919250A2 (pt) | 2015-12-15 |
| CN102215865A (zh) | 2011-10-12 |
| EP2334328A4 (en) | 2012-08-29 |
| CN102215865B (zh) | 2013-10-30 |
| WO2010036774A1 (en) | 2010-04-01 |
| AU2009296628A1 (en) | 2010-04-01 |
| JP2012503489A (ja) | 2012-02-09 |
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